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The map label for this gene is thiE

Identifier: 114797291

GI number: 114797291

Start: 3359940

End: 3360605

Strand: Direct

Name: thiE

Synonym: HNE_3185

Alternate gene names: 114797291

Gene position: 3359940-3360605 (Clockwise)

Preceding gene: 114799812

Following gene: 114797941

Centisome position: 90.69

GC content: 66.97

Gene sequence:

>666_bases
ATGACTGACACCGCCCAACCCCGCCCGCGCACACGCCTGTACCTGATCACACCGCCACAGATCGGCGATGTGTCCGCCTT
CCGGAAATCACTGGAGGAAACCCTCAGCGCCGGGGATGTGGCCTCATTGCAGCTGCGCCTCAAGGGCCAGGACGGCATGA
TAGATATGCAGGCCACGCAAGAGGTCGGCGCCGCCGTCACTGCGATGGCCCAGGAAGCCGGCGTCGCGGTCCTGATCAAC
GACGCCGCAGAGCTCTCCCGGCAGCTGGGCGCAGACGGGGTGCATCTCGGCTGGGACGACATGCCGGTGAAGACCGCGCG
CGCCCTGCTCGGCCCGGACGCGATCATCGGCGCCACCGCCAAGAATTCCTATCACCGCGCCATGCAGGCGGGCGAAGACG
GCGCCGACTATGTCGCCTTCGGCGCCTTCTATCCCACCACCACCAAGGAAGGCACCATACCTGCCGAGCTGGAGCTGCTG
GAAGTCTGGCAGAGCGCGATGATCCTGCCCTGCGTGGCGATCGGGGGCATCACCGTCTCCAATGCGGCCCCGCTCGTGAC
AGCAGGCGCAGACTTCCTGGCCGTCTCCAGCGGGGTCTGGAACCATGAAGACGGGCCAGCCGCCGCCGTCCGGGCCTTCA
ACGCCCTCTTCGAGTCGCTGGCTTAA

Upstream 100 bases:

>100_bases
CCGGCTTGCTTTAAAGTTCCAATATAGAACTTTCATTCCTTTTCGCCAGAGGCATGCCCTCCGTTGACTTCCCCTCGCCC
TCGCCGCAGAGGCTGCAGCC

Downstream 100 bases:

>100_bases
ACTGGCCTCAGGCGCGCGCTCAGAGCGCAGGCGGGGTCTTGGCCACCTTGATGTCGCGCAGGTCGTCCGCCATGCGGAAC
AGCAGCATGTAAAGCTCGCA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTDTAQPRPRTRLYLITPPQIGDVSAFRKSLEETLSAGDVASLQLRLKGQDGMIDMQATQEVGAAVTAMAQEAGVAVLIN
DAAELSRQLGADGVHLGWDDMPVKTARALLGPDAIIGATAKNSYHRAMQAGEDGADYVAFGAFYPTTTKEGTIPAELELL
EVWQSAMILPCVAIGGITVSNAAPLVTAGADFLAVSSGVWNHEDGPAAAVRAFNALFESLA

Sequences:

>Translated_221_residues
MTDTAQPRPRTRLYLITPPQIGDVSAFRKSLEETLSAGDVASLQLRLKGQDGMIDMQATQEVGAAVTAMAQEAGVAVLIN
DAAELSRQLGADGVHLGWDDMPVKTARALLGPDAIIGATAKNSYHRAMQAGEDGADYVAFGAFYPTTTKEGTIPAELELL
EVWQSAMILPCVAIGGITVSNAAPLVTAGADFLAVSSGVWNHEDGPAAAVRAFNALFESLA
>Mature_220_residues
TDTAQPRPRTRLYLITPPQIGDVSAFRKSLEETLSAGDVASLQLRLKGQDGMIDMQATQEVGAAVTAMAQEAGVAVLIND
AAELSRQLGADGVHLGWDDMPVKTARALLGPDAIIGATAKNSYHRAMQAGEDGADYVAFGAFYPTTTKEGTIPAELELLE
VWQSAMILPCVAIGGITVSNAAPLVTAGADFLAVSSGVWNHEDGPAAAVRAFNALFESLA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=148, Percent_Identity=33.1081081081081, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_HYPNA (Q0BXD3)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761860.1
- ProteinModelPortal:   Q0BXD3
- STRING:   Q0BXD3
- GeneID:   4289215
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3185
- NMPDR:   fig|228405.5.peg.3050
- TIGR:   HNE_3185
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   FIVNDSI
- ProtClustDB:   CLSK891382
- BioCyc:   HNEP81032:HNE_3185-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23083; Mature: 22952

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTAQPRPRTRLYLITPPQIGDVSAFRKSLEETLSAGDVASLQLRLKGQDGMIDMQATQ
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEHHHHH
EVGAAVTAMAQEAGVAVLINDAAELSRQLGADGVHLGWDDMPVKTARALLGPDAIIGATA
HHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCEEECCC
KNSYHRAMQAGEDGADYVAFGAFYPTTTKEGTIPAELELLEVWQSAMILPCVAIGGITVS
CHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEC
NAAPLVTAGADFLAVSSGVWNHEDGPAAAVRAFNALFESLA
CCCCEEECCCHHEEECCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDTAQPRPRTRLYLITPPQIGDVSAFRKSLEETLSAGDVASLQLRLKGQDGMIDMQATQ
CCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEHHHHH
EVGAAVTAMAQEAGVAVLINDAAELSRQLGADGVHLGWDDMPVKTARALLGPDAIIGATA
HHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCEEECCC
KNSYHRAMQAGEDGADYVAFGAFYPTTTKEGTIPAELELLEVWQSAMILPCVAIGGITVS
CHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEC
NAAPLVTAGADFLAVSSGVWNHEDGPAAAVRAFNALFESLA
CCCCEEECCCHHEEECCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA