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The map label for this gene is rimK1 [H]

Identifier: 114797265

GI number: 114797265

Start: 2721740

End: 2723209

Strand: Direct

Name: rimK1 [H]

Synonym: HNE_2569

Alternate gene names: 114797265

Gene position: 2721740-2723209 (Clockwise)

Preceding gene: 114800022

Following gene: 114797509

Centisome position: 73.46

GC content: 61.56

Gene sequence:

>1470_bases
ATGACAGATTGGGTCGTGCTTGTTGAATCGGCAAGCGACATCTCTCAAGCCGAGACGCCGCACAAGGTTCTGCGTATTTC
AGACTATATCTCAAAGCCTGCGCTGTTCACCGGCCGCAGGCCCTACATCCTCAATCTTGCGCGCTCCTATTCCTACCAGT
CGGAAGGCTATTACGCCTCCCTCTTGGCTGAGGCGCGCGGCCACCGGATCAGCCCCAGCGTCCAGACGATGGTCGAGCTC
TCAAAGAAGACGCTCTACAACCACGCCCTGCCAGATCTCGGCGAAACCCTGCAGGAAGCCCTCGCCAAGGGCGCCCCGAA
GGTCGAGACCCTGTTCGTCGCCTTCTCGCGCACCGAACTCCCAGCCTATGAACGCCTCGCCCGCGAAGCCATGGACTGGT
TCCGCACCCCCGCCCTCGAAATCGAGTTCGACGAGGAAGCCCCCCACGGCATCGGCCGCATCCGCGCCGTCCCGCCCCAC
AAGCTGAAAGACCAGCGCCGCCAATTCTTCCTGGACTCGATGGAGGCCTACACCAAAGGCCGCGTCAAAAACGAGAAGAC
CCGCACCCCCGCCAAATGGGCCCTCGCCGTGCTGGTCGACCCCAATGAGAAGACCGCGCCGTCAAAGCCCGCCTCCCTCA
AACGCCTGGCCGATGTCGCTGAAAAGATGGGCGTCGAGGTCGAGCTGATCGACCCGACCGACCTGCCATCGCTCGCCGAA
TTCGACGCCCTCTTCATTCGCGCCACCACCTCGATCGACAATTTCACCTACCGCTTCGCCCGCCGCGCCGAGCAGGAAGG
CATGCCCGTCATCGACGACACCCATTCGATGATCCGCTGCACCAACAAGGTCTATCTCAAAGAGATTCTAGAGAAGGCCG
GCCTGCCCATTCCGCGTACGGAAATTGTGGACGAGAAGACAGACCTCTCCGGCCTGTTTGACCGCCTCGGCAGCCCGGTC
ATCCTGAAGACCCCCGATGGCAGCTTCGGCATCAATATGGTCAAGGCCCAGTCGCTCGACGAGCTGAAAACCGGCGCCAA
GAAAATGTTCGAGGACACCGCCCTCATTATCGCCCAGGAATTCCGCCCCACGAAATTCGACTGGCGCATCGGCGTCCTGA
ATGGCGAGCCCCTCTACGCCTGCCAATACCGCATGGCCCGCGGCCACTGGCAGACGGTGAAATATGGTGAGGGTGGCAAA
TCCACCGAAGGCGGCTTCACCACCATGCCGGTGGAAGACGCGCCGCCTGAAGTCGTCAACGTCGCCGTCCGCGCCGCCAG
CCTCATCGGCGACGGCCTCTATGGCGTAGACCTCAAAGAAACCGAAGAGGGCGTCATCATCATCGAAATCAACGACAACC
CGTCGATTGACCATGATGTCGAAGGCGCCGTCCTGAAAGATGAAATCTGGAGACGGATCATCTCCTGGTTCTCCACGCGC
CTAGAGCGCCGCATGGGCCGCGCGGGCTGA

Upstream 100 bases:

>100_bases
GCGGTTATCGCGTAATTGCGACCCTTGAGTCCTACTATCCGGACGGAGAAGCGGCGCTGCGGATGGAGAAGTATCTCGAT
GCGCCCTCTAATGGAGCCCC

Downstream 100 bases:

>100_bases
AGAGCCCCCCGGCGAACGCGTTCGCTCTCACCCGCACAACCTGATCCCCTCCCCCAGACATGGGGGAGGGCTAGGGTGGG
GGCGCTCTCAACCCGGCAAC

Product: alpha-L-glutamate ligase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTDWVVLVESASDISQAETPHKVLRISDYISKPALFTGRRPYILNLARSYSYQSEGYYASLLAEARGHRISPSVQTMVEL
SKKTLYNHALPDLGETLQEALAKGAPKVETLFVAFSRTELPAYERLAREAMDWFRTPALEIEFDEEAPHGIGRIRAVPPH
KLKDQRRQFFLDSMEAYTKGRVKNEKTRTPAKWALAVLVDPNEKTAPSKPASLKRLADVAEKMGVEVELIDPTDLPSLAE
FDALFIRATTSIDNFTYRFARRAEQEGMPVIDDTHSMIRCTNKVYLKEILEKAGLPIPRTEIVDEKTDLSGLFDRLGSPV
ILKTPDGSFGINMVKAQSLDELKTGAKKMFEDTALIIAQEFRPTKFDWRIGVLNGEPLYACQYRMARGHWQTVKYGEGGK
STEGGFTTMPVEDAPPEVVNVAVRAASLIGDGLYGVDLKETEEGVIIIEINDNPSIDHDVEGAVLKDEIWRRIISWFSTR
LERRMGRAG

Sequences:

>Translated_489_residues
MTDWVVLVESASDISQAETPHKVLRISDYISKPALFTGRRPYILNLARSYSYQSEGYYASLLAEARGHRISPSVQTMVEL
SKKTLYNHALPDLGETLQEALAKGAPKVETLFVAFSRTELPAYERLAREAMDWFRTPALEIEFDEEAPHGIGRIRAVPPH
KLKDQRRQFFLDSMEAYTKGRVKNEKTRTPAKWALAVLVDPNEKTAPSKPASLKRLADVAEKMGVEVELIDPTDLPSLAE
FDALFIRATTSIDNFTYRFARRAEQEGMPVIDDTHSMIRCTNKVYLKEILEKAGLPIPRTEIVDEKTDLSGLFDRLGSPV
ILKTPDGSFGINMVKAQSLDELKTGAKKMFEDTALIIAQEFRPTKFDWRIGVLNGEPLYACQYRMARGHWQTVKYGEGGK
STEGGFTTMPVEDAPPEVVNVAVRAASLIGDGLYGVDLKETEEGVIIIEINDNPSIDHDVEGAVLKDEIWRRIISWFSTR
LERRMGRAG
>Mature_488_residues
TDWVVLVESASDISQAETPHKVLRISDYISKPALFTGRRPYILNLARSYSYQSEGYYASLLAEARGHRISPSVQTMVELS
KKTLYNHALPDLGETLQEALAKGAPKVETLFVAFSRTELPAYERLAREAMDWFRTPALEIEFDEEAPHGIGRIRAVPPHK
LKDQRRQFFLDSMEAYTKGRVKNEKTRTPAKWALAVLVDPNEKTAPSKPASLKRLADVAEKMGVEVELIDPTDLPSLAEF
DALFIRATTSIDNFTYRFARRAEQEGMPVIDDTHSMIRCTNKVYLKEILEKAGLPIPRTEIVDEKTDLSGLFDRLGSPVI
LKTPDGSFGINMVKAQSLDELKTGAKKMFEDTALIIAQEFRPTKFDWRIGVLNGEPLYACQYRMARGHWQTVKYGEGGKS
TEGGFTTMPVEDAPPEVVNVAVRAASLIGDGLYGVDLKETEEGVIIIEINDNPSIDHDVEGAVLKDEIWRRIISWFSTRL
ERRMGRAG

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=264, Percent_Identity=24.6212121212121, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 54912; Mature: 54781

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDWVVLVESASDISQAETPHKVLRISDYISKPALFTGRRPYILNLARSYSYQSEGYYAS
CCCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCEECCCCCCEEEHHHHCCCCCCCHHHH
LLAEARGHRISPSVQTMVELSKKTLYNHALPDLGETLQEALAKGAPKVETLFVAFSRTEL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCC
PAYERLAREAMDWFRTPALEIEFDEEAPHGIGRIRAVPPHKLKDQRRQFFLDSMEAYTKG
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
RVKNEKTRTPAKWALAVLVDPNEKTAPSKPASLKRLADVAEKMGVEVELIDPTDLPSLAE
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHH
FDALFIRATTSIDNFTYRFARRAEQEGMPVIDDTHSMIRCTNKVYLKEILEKAGLPIPRT
HCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
EIVDEKTDLSGLFDRLGSPVILKTPDGSFGINMVKAQSLDELKTGAKKMFEDTALIIAQE
HHCCCCCHHHHHHHHCCCCEEEECCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHEEECC
FRPTKFDWRIGVLNGEPLYACQYRMARGHWQTVKYGEGGKSTEGGFTTMPVEDAPPEVVN
CCCCCCEEEEEEECCCCEEHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHH
VAVRAASLIGDGLYGVDLKETEEGVIIIEINDNPSIDHDVEGAVLKDEIWRRIISWFSTR
HHHHHHHHHCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LERRMGRAG
HHHHHCCCC
>Mature Secondary Structure 
TDWVVLVESASDISQAETPHKVLRISDYISKPALFTGRRPYILNLARSYSYQSEGYYAS
CCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCEECCCCCCEEEHHHHCCCCCCCHHHH
LLAEARGHRISPSVQTMVELSKKTLYNHALPDLGETLQEALAKGAPKVETLFVAFSRTEL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCC
PAYERLAREAMDWFRTPALEIEFDEEAPHGIGRIRAVPPHKLKDQRRQFFLDSMEAYTKG
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
RVKNEKTRTPAKWALAVLVDPNEKTAPSKPASLKRLADVAEKMGVEVELIDPTDLPSLAE
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHH
FDALFIRATTSIDNFTYRFARRAEQEGMPVIDDTHSMIRCTNKVYLKEILEKAGLPIPRT
HCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
EIVDEKTDLSGLFDRLGSPVILKTPDGSFGINMVKAQSLDELKTGAKKMFEDTALIIAQE
HHCCCCCHHHHHHHHCCCCEEEECCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHEEECC
FRPTKFDWRIGVLNGEPLYACQYRMARGHWQTVKYGEGGKSTEGGFTTMPVEDAPPEVVN
CCCCCCEEEEEEECCCCEEHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHH
VAVRAASLIGDGLYGVDLKETEEGVIIIEINDNPSIDHDVEGAVLKDEIWRRIISWFSTR
HHHHHHHHHCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LERRMGRAG
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA