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The map label for this gene is purH [H]

Identifier: 114797222

GI number: 114797222

Start: 3516969

End: 3518558

Strand: Direct

Name: purH [H]

Synonym: HNE_3372

Alternate gene names: 114797222

Gene position: 3516969-3518558 (Clockwise)

Preceding gene: 114797226

Following gene: 114797976

Centisome position: 94.92

GC content: 66.79

Gene sequence:

>1590_bases
ATGTCCGGCGCCCCCGTCACCCTCCGCCGCGCGCTTATCTCCGTGTCCGACAAGACCGGCCTGATCGAGCAGGCAAAGCG
CCTCGCCGCCCTCGGTGTCGAGCTTGTCTCTACGGGGGGCACCTCCAAGGCGCTGAAAGACGCCGGCCTCGCGGTCAAAG
ACGTGTCAGACCTCACCGGCTTCCCCGAAATGATGGACGGGCGCGTCAAGACGCTCCACCCGGCCGTGCATGGCGGCCTC
CTTTACCTGCGCGACAACCCCTCCCACGTGAAAGACGCGGCAGACCACGGCATCGGCGCCATCGACCTGATCTACGTCAA
CCTCTACCCCTTCGAGGCCACGGTGGCTTCTGGCGCAGACTTTGAAACCTGCATCGAGAATATCGACATCGGTGGCCCCG
CCATGCTGCGCGCCGCCGCCAAGAACGGCGCCTTCGTGGCCGTCTGCACCGATGGCGGCGATGTCGACGCCGTCCTCGCC
CAGGCCGAGGCCAATGCCGGCGCGGTGAGCCACGCCCTCCTCCGCCGCCTCGCCGCGAAAACTTACGCCCGCACCGCCGC
CTATGACGCGGCCATTTCCGGCTGGTACGCCAAGCAGATCGGCGAGGACGCGCCGGACTGGGCCGCCATTGGTGGCAAGC
TCCAGCAATCCCTGCGCTATGGCGAAAACCCCCACCAGAAAGCCGCCTTCTACGTCACCGGCGAAAAGCGCCCGGGCGTG
GCCACCGCGCGCCAGCTTCAGGGTAAGGAGCTTTCCTACAACAACATCAACGACACCGACGCGGCCTATGAGCTGATCGG
GGAGCTGGGCAGCGACAAACCCGCCGTCGCCATCATCAAGCACGCCAACCCCTGCGGCGTGGCGCTCGGCCCGAACATCA
TCGAAGCCTACAAAGCGGCCCTCGCCTGCGACCCCACATCGGCCTTCGGCGGCATCGTCGCCCTCAACCGCCCGCTGGAC
GAGGAAACGGCGAAGGTGATCACAGAGATCTTCACCGAAGTCGTCATCGCGCCGGGCGCGGACGCGGCAGCTGAAGCGAT
CTTTGCCAAGAAGAAAAACCTCCGCCTCCTGATCACTGACGACCTGCCCGACCCGGCCACCACCGGCACGTTCGTGAAAA
CCGTCGCCGGCGGCTTCCTGCTGCAAGACCGCGACGCGGGCCGCATCCACCTTGACGACCTTGAAGTCGTCACCAAACGC
GCGCCCACCGCGCAGGAACTGGAAGACATGCTGTTTGCCTGGCGCGTCGCCAAGCATGTGAAATCCAACACCATCGTCTA
CGCGAAAGACGGCGTCACCGCCGGCATCGGCGCCGGCCAGATGAGCCGCATCGACAGCGCCCGCATCGCCGCCCGCAAGG
CCCAGGACGCCCAGGAAACCGCCGGCTGGTCCGACCCGCGCACCAAAGGCTGCGTCGCCGCCTCCGACGCCTTCTTTCCG
TTTGCGGATGGTTTGTTGCAGGCGGCCTCAGCCGGCGCCACCGCCGTCATCCAACCGGGCGGCTCAATCCGCGACGACGA
AGTGATCGCCGCGGCGGACGAGGCGGGGCTCGCGATGGTGTTCACGGGGATGCGGCACTTCAGGCATTGA

Upstream 100 bases:

>100_bases
CGCCTGAGACGGCCTGATACGGTCCGGCATGTCTTGACGCCGCCTCAGGGGGTGCTAGGCCGCGCGCAGCTTTCCCAACG
CCTTTGAAGGAGCAGCCCAT

Downstream 100 bases:

>100_bases
GGCCGGTGGGCTGATCCCAGCCGATTCAATCCAGTTGCAATCTAGCGCATGTTGCGTCACCCCATGATTGGGGAGGGACC
GGCATGACACGGGGATTACT

Product: bifunctional purine biosynthesis protein PurH

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSGAPVTLRRALISVSDKTGLIEQAKRLAALGVELVSTGGTSKALKDAGLAVKDVSDLTGFPEMMDGRVKTLHPAVHGGL
LYLRDNPSHVKDAADHGIGAIDLIYVNLYPFEATVASGADFETCIENIDIGGPAMLRAAAKNGAFVAVCTDGGDVDAVLA
QAEANAGAVSHALLRRLAAKTYARTAAYDAAISGWYAKQIGEDAPDWAAIGGKLQQSLRYGENPHQKAAFYVTGEKRPGV
ATARQLQGKELSYNNINDTDAAYELIGELGSDKPAVAIIKHANPCGVALGPNIIEAYKAALACDPTSAFGGIVALNRPLD
EETAKVITEIFTEVVIAPGADAAAEAIFAKKKNLRLLITDDLPDPATTGTFVKTVAGGFLLQDRDAGRIHLDDLEVVTKR
APTAQELEDMLFAWRVAKHVKSNTIVYAKDGVTAGIGAGQMSRIDSARIAARKAQDAQETAGWSDPRTKGCVAASDAFFP
FADGLLQAASAGATAVIQPGGSIRDDEVIAAADEAGLAMVFTGMRHFRH

Sequences:

>Translated_529_residues
MSGAPVTLRRALISVSDKTGLIEQAKRLAALGVELVSTGGTSKALKDAGLAVKDVSDLTGFPEMMDGRVKTLHPAVHGGL
LYLRDNPSHVKDAADHGIGAIDLIYVNLYPFEATVASGADFETCIENIDIGGPAMLRAAAKNGAFVAVCTDGGDVDAVLA
QAEANAGAVSHALLRRLAAKTYARTAAYDAAISGWYAKQIGEDAPDWAAIGGKLQQSLRYGENPHQKAAFYVTGEKRPGV
ATARQLQGKELSYNNINDTDAAYELIGELGSDKPAVAIIKHANPCGVALGPNIIEAYKAALACDPTSAFGGIVALNRPLD
EETAKVITEIFTEVVIAPGADAAAEAIFAKKKNLRLLITDDLPDPATTGTFVKTVAGGFLLQDRDAGRIHLDDLEVVTKR
APTAQELEDMLFAWRVAKHVKSNTIVYAKDGVTAGIGAGQMSRIDSARIAARKAQDAQETAGWSDPRTKGCVAASDAFFP
FADGLLQAASAGATAVIQPGGSIRDDEVIAAADEAGLAMVFTGMRHFRH
>Mature_528_residues
SGAPVTLRRALISVSDKTGLIEQAKRLAALGVELVSTGGTSKALKDAGLAVKDVSDLTGFPEMMDGRVKTLHPAVHGGLL
YLRDNPSHVKDAADHGIGAIDLIYVNLYPFEATVASGADFETCIENIDIGGPAMLRAAAKNGAFVAVCTDGGDVDAVLAQ
AEANAGAVSHALLRRLAAKTYARTAAYDAAISGWYAKQIGEDAPDWAAIGGKLQQSLRYGENPHQKAAFYVTGEKRPGVA
TARQLQGKELSYNNINDTDAAYELIGELGSDKPAVAIIKHANPCGVALGPNIIEAYKAALACDPTSAFGGIVALNRPLDE
ETAKVITEIFTEVVIAPGADAAAEAIFAKKKNLRLLITDDLPDPATTGTFVKTVAGGFLLQDRDAGRIHLDDLEVVTKRA
PTAQELEDMLFAWRVAKHVKSNTIVYAKDGVTAGIGAGQMSRIDSARIAARKAQDAQETAGWSDPRTKGCVAASDAFFPF
ADGLLQAASAGATAVIQPGGSIRDDEVIAAADEAGLAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=473, Percent_Identity=40.5919661733615, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI1790439, Length=535, Percent_Identity=52.8971962616822, Blast_Score=513, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71985564, Length=475, Percent_Identity=38.1052631578947, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI71985574, Length=305, Percent_Identity=31.8032786885246, Blast_Score=118, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6323768, Length=476, Percent_Identity=37.8151260504202, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6323056, Length=477, Percent_Identity=37.7358490566038, Blast_Score=260, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24649832, Length=472, Percent_Identity=38.5593220338983, Blast_Score=260, Evalue=1e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55402; Mature: 55271

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAPVTLRRALISVSDKTGLIEQAKRLAALGVELVSTGGTSKALKDAGLAVKDVSDLTG
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHCCCEEECHHHHCC
FPEMMDGRVKTLHPAVHGGLLYLRDNPSHVKDAADHGIGAIDLIYVNLYPFEATVASGAD
CHHHHCCCHHEECHHHHCCEEEEECCCHHHHHHHHCCCCEEEEEEEEEEECHHHHCCCCC
FETCIENIDIGGPAMLRAAAKNGAFVAVCTDGGDVDAVLAQAEANAGAVSHALLRRLAAK
HHHHHHHCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
TYARTAAYDAAISGWYAKQIGEDAPDWAAIGGKLQQSLRYGENPHQKAAFYVTGEKRPGV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
ATARQLQGKELSYNNINDTDAAYELIGELGSDKPAVAIIKHANPCGVALGPNIIEAYKAA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
LACDPTSAFGGIVALNRPLDEETAKVITEIFTEVVIAPGADAAAEAIFAKKKNLRLLITD
HCCCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCEEEEEC
DLPDPATTGTFVKTVAGGFLLQDRDAGRIHLDDLEVVTKRAPTAQELEDMLFAWRVAKHV
CCCCCCCCCHHHHHHHCCEEEECCCCCEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KSNTIVYAKDGVTAGIGAGQMSRIDSARIAARKAQDAQETAGWSDPRTKGCVAASDAFFP
CCCEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCH
FADGLLQAASAGATAVIQPGGSIRDDEVIAAADEAGLAMVFTGMRHFRH
HHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SGAPVTLRRALISVSDKTGLIEQAKRLAALGVELVSTGGTSKALKDAGLAVKDVSDLTG
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHCCCEEECHHHHCC
FPEMMDGRVKTLHPAVHGGLLYLRDNPSHVKDAADHGIGAIDLIYVNLYPFEATVASGAD
CHHHHCCCHHEECHHHHCCEEEEECCCHHHHHHHHCCCCEEEEEEEEEEECHHHHCCCCC
FETCIENIDIGGPAMLRAAAKNGAFVAVCTDGGDVDAVLAQAEANAGAVSHALLRRLAAK
HHHHHHHCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
TYARTAAYDAAISGWYAKQIGEDAPDWAAIGGKLQQSLRYGENPHQKAAFYVTGEKRPGV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
ATARQLQGKELSYNNINDTDAAYELIGELGSDKPAVAIIKHANPCGVALGPNIIEAYKAA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
LACDPTSAFGGIVALNRPLDEETAKVITEIFTEVVIAPGADAAAEAIFAKKKNLRLLITD
HCCCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCEEEEEC
DLPDPATTGTFVKTVAGGFLLQDRDAGRIHLDDLEVVTKRAPTAQELEDMLFAWRVAKHV
CCCCCCCCCHHHHHHHCCEEEECCCCCEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KSNTIVYAKDGVTAGIGAGQMSRIDSARIAARKAQDAQETAGWSDPRTKGCVAASDAFFP
CCCEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCH
FADGLLQAASAGATAVIQPGGSIRDDEVIAAADEAGLAMVFTGMRHFRH
HHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA