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The map label for this gene is priA [H]

Identifier: 114797126

GI number: 114797126

Start: 1962664

End: 1964862

Strand: Reverse

Name: priA [H]

Synonym: HNE_1896

Alternate gene names: 114797126

Gene position: 1964862-1962664 (Counterclockwise)

Preceding gene: 114800225

Following gene: 114798041

Centisome position: 53.03

GC content: 65.26

Gene sequence:

>2199_bases
ATGGCCGTCGCCCGAATCCTCTTCACCCTGCCACTGCCCGAACCGTTCGATTACGCGGTTCCCGAGGGCATGACGCTTCA
GCCCGGAAGCTATGTTGCCGCGCCGGTCGGTCCGCATGACCGGCTCGGTATCGTGGTCGACGTGCTGGGTGATGACGTGG
CCACCGGGCGCAAGCTCAAGACGGTCACGGCCGTCTATGACGCCCCGCCGATGGGCGCTCCGATGCGCGAGTTCCTGGCA
TGGGTCGCGCGTTACACGGTCACCCATCCGGGGCAGGTTCTGGCGATGGCGCTGCGGGCGCGGGAGGGGCTTTTGCCCTC
GCCGGTCGAAACCCGATACCGCGCGACCGGCAAGCTGCCGGATAGGATGACGCCGGCGCGCCAGAAGGTGCTGGAGGTGT
CCCGAACTGTCCCGAACTGTCCCGATTTGTCCAGAAATGACCAGCTCTGGTCGGCTGTTGACCTTGCCAGTGCAGCAAAT
GTCTCGTCCGGCGTAGTCAAAGGACTGGCCGAGGCGGGCGTGCTGGAGGCGGTCGAATTGGAGACCGACCCGTCTTTCGC
TATCCCCGATCCAGATCGCAAAGGCGCCGTTCTGACCGGCGAACAGGCCGTCGCGGCGGGGGAGTTGAAGGCCGCTATCC
ACGCCGGCGGCTTCTCGGCCTTCCTGCTGGACGGCGTGACAGGCTCGGGCAAAACGGAAGTCTATTTCGAGGCGATTGCT
GAAACGCTGAAATCTGACCCCGAGGCGCAGATCCTTGTTCTGTTGCCGGAAATTGCTCTGACGCAGGCGATCCTCGCCCG
CTTCGAAGCCCGCTTTGGCGCGGCGCCTGCGCCATGGCATTCCGGCCTCAGCCCCAAGGATCGCCGCCGCACCTGGCGTG
AAGCTGTCCACGGGAGAGCCCGCATCGTCATCGGTGCCCGCTCAGCCCTTTTCCTGCCCTATCGCAAGCTGCGCCTCATC
ATCGTCGACGAGGAACACGACACCTCCTTCAAGCAGGAAGACGGCGTCACCTACCACGCCCGCGATCTGGCGGTGATGCG
CGCCAAGCTGGAGGGGGGGCAAGTCATCCTCGCCTCCGCAACGCCAGCGCTGGAAACTGCCGTGAATGCTGAGGCCGGGC
GATATGTACACCTCAGGCTCGCCGCGCGCCCCGGTGCCTCGCGCCTGCCGGATATTGAGCTGGTCGATCTGCGAACCGCC
CCCCCGGGGAAAGGCAAGTGGCTATCCCCCGTGCTTGAGCGGGCGTTGATGGAGACCCTCGCCAATGGGGAGCAGTCGCT
CCTCTTCCTCAACCGGCGCGGCTATGCCCCGCTGGTCATCTGCAAGGCCTGCGGGGAAAGGCTGAAGACGCCCGGCACCG
AGAACTGGCTGACCGAGCACCGGTACTCCGGCCGCCTCGTCTGCCACGTTACCGGCTATTCCATCCCCAAGCCCACCAAA
TGCCCCCAATGCGGTGCGCCCGATTCGCTGATGGGCGTCGGGCCGGGCGTCGAGCGGGTGGCCGAAGAGGTTCGGACCTT
GCTGCCCCATGCCCGAATCGAGATTTTCTCTTCAGATACAGCGCAGGGCGCCGATGTTACCCGCAGTCTCGTGGAGCGTA
TGGAGCGCAGCGAGATCGACGTTATGATCGGCACGCAGATCGTGGCCAAAGGGCACAACTTCCCCAATCTGACGCTGGTG
GGCGTCGTGGACGCCGATAGCGGCCTCAAGGGCGGGGATCTGCGCGCGGGCGAGCGCACCTACCAGCTGCTGAGCCAGGT
GGCGGGCCGGGCAGGGCGCGCAGAGCGCCCCGGCCGGGCATTGGTGCAGACTTATGCCCCGGATAACCCCGCCATGGAGG
CGCTCGCCGCCAATGACCGCGACGGCTTCCTGCAGATCGAGCGGGATGTCCGCTCCGAACTTGTCCTGCCCCCTTTCGGC
CGGCTGGCAGCAATGATTCTGTCGGCGCCGTCTGCAGATCTTGTGGATAAAGCGGCTTTGGATATCGCCGCCGCTGCGCC
CAACGGCGATGGCATAGAGGTGTTTGGTCCGGCGCCGGCGCCGATTACGGTGCTGCGGGGCCGGCATCGCCGGCGGTTCC
TGGTAAAGAGCCCGAGAATCATTGATCTTTCAGCCTATATGGCCGCCTGGACCGCCCGCCTGAGACTCCCGTCTCAGGTG
CGGATTTCGATCGATATAGACCCATATTCTTTTCTCTGA

Upstream 100 bases:

>100_bases
CCGCCGAGGCAAAGCGCCTCTTCGAGGCCGCGCAAACCTGATGCCGGCCTGAGTGCTCCGATGGGATGACGACACCCGGT
CAAAGGTCTAAGCCGGGCCC

Downstream 100 bases:

>100_bases
ATGCCCTTTCCCCTCGTAAGGCCTCACTCGCGGCCATTTCGCCGCATGACGCCTGACGGCGGGGCTGTTGTCAGCGCGAA
TTTCTAATGATAAGCGCCGC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 732; Mature: 731

Protein sequence:

>732_residues
MAVARILFTLPLPEPFDYAVPEGMTLQPGSYVAAPVGPHDRLGIVVDVLGDDVATGRKLKTVTAVYDAPPMGAPMREFLA
WVARYTVTHPGQVLAMALRAREGLLPSPVETRYRATGKLPDRMTPARQKVLEVSRTVPNCPDLSRNDQLWSAVDLASAAN
VSSGVVKGLAEAGVLEAVELETDPSFAIPDPDRKGAVLTGEQAVAAGELKAAIHAGGFSAFLLDGVTGSGKTEVYFEAIA
ETLKSDPEAQILVLLPEIALTQAILARFEARFGAAPAPWHSGLSPKDRRRTWREAVHGRARIVIGARSALFLPYRKLRLI
IVDEEHDTSFKQEDGVTYHARDLAVMRAKLEGGQVILASATPALETAVNAEAGRYVHLRLAARPGASRLPDIELVDLRTA
PPGKGKWLSPVLERALMETLANGEQSLLFLNRRGYAPLVICKACGERLKTPGTENWLTEHRYSGRLVCHVTGYSIPKPTK
CPQCGAPDSLMGVGPGVERVAEEVRTLLPHARIEIFSSDTAQGADVTRSLVERMERSEIDVMIGTQIVAKGHNFPNLTLV
GVVDADSGLKGGDLRAGERTYQLLSQVAGRAGRAERPGRALVQTYAPDNPAMEALAANDRDGFLQIERDVRSELVLPPFG
RLAAMILSAPSADLVDKAALDIAAAAPNGDGIEVFGPAPAPITVLRGRHRRRFLVKSPRIIDLSAYMAAWTARLRLPSQV
RISIDIDPYSFL

Sequences:

>Translated_732_residues
MAVARILFTLPLPEPFDYAVPEGMTLQPGSYVAAPVGPHDRLGIVVDVLGDDVATGRKLKTVTAVYDAPPMGAPMREFLA
WVARYTVTHPGQVLAMALRAREGLLPSPVETRYRATGKLPDRMTPARQKVLEVSRTVPNCPDLSRNDQLWSAVDLASAAN
VSSGVVKGLAEAGVLEAVELETDPSFAIPDPDRKGAVLTGEQAVAAGELKAAIHAGGFSAFLLDGVTGSGKTEVYFEAIA
ETLKSDPEAQILVLLPEIALTQAILARFEARFGAAPAPWHSGLSPKDRRRTWREAVHGRARIVIGARSALFLPYRKLRLI
IVDEEHDTSFKQEDGVTYHARDLAVMRAKLEGGQVILASATPALETAVNAEAGRYVHLRLAARPGASRLPDIELVDLRTA
PPGKGKWLSPVLERALMETLANGEQSLLFLNRRGYAPLVICKACGERLKTPGTENWLTEHRYSGRLVCHVTGYSIPKPTK
CPQCGAPDSLMGVGPGVERVAEEVRTLLPHARIEIFSSDTAQGADVTRSLVERMERSEIDVMIGTQIVAKGHNFPNLTLV
GVVDADSGLKGGDLRAGERTYQLLSQVAGRAGRAERPGRALVQTYAPDNPAMEALAANDRDGFLQIERDVRSELVLPPFG
RLAAMILSAPSADLVDKAALDIAAAAPNGDGIEVFGPAPAPITVLRGRHRRRFLVKSPRIIDLSAYMAAWTARLRLPSQV
RISIDIDPYSFL
>Mature_731_residues
AVARILFTLPLPEPFDYAVPEGMTLQPGSYVAAPVGPHDRLGIVVDVLGDDVATGRKLKTVTAVYDAPPMGAPMREFLAW
VARYTVTHPGQVLAMALRAREGLLPSPVETRYRATGKLPDRMTPARQKVLEVSRTVPNCPDLSRNDQLWSAVDLASAANV
SSGVVKGLAEAGVLEAVELETDPSFAIPDPDRKGAVLTGEQAVAAGELKAAIHAGGFSAFLLDGVTGSGKTEVYFEAIAE
TLKSDPEAQILVLLPEIALTQAILARFEARFGAAPAPWHSGLSPKDRRRTWREAVHGRARIVIGARSALFLPYRKLRLII
VDEEHDTSFKQEDGVTYHARDLAVMRAKLEGGQVILASATPALETAVNAEAGRYVHLRLAARPGASRLPDIELVDLRTAP
PGKGKWLSPVLERALMETLANGEQSLLFLNRRGYAPLVICKACGERLKTPGTENWLTEHRYSGRLVCHVTGYSIPKPTKC
PQCGAPDSLMGVGPGVERVAEEVRTLLPHARIEIFSSDTAQGADVTRSLVERMERSEIDVMIGTQIVAKGHNFPNLTLVG
VVDADSGLKGGDLRAGERTYQLLSQVAGRAGRAERPGRALVQTYAPDNPAMEALAANDRDGFLQIERDVRSELVLPPFGR
LAAMILSAPSADLVDKAALDIAAAAPNGDGIEVFGPAPAPITVLRGRHRRRFLVKSPRIIDLSAYMAAWTARLRLPSQVR
ISIDIDPYSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=753, Percent_Identity=35.3253652058433, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 78951; Mature: 78819

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVARILFTLPLPEPFDYAVPEGMTLQPGSYVAAPVGPHDRLGIVVDVLGDDVATGRKLK
CCCEEEEEECCCCCCCCCCCCCCCEECCCCEEEECCCCCCCCCEEEEECCCCCCCCCEEE
TVTAVYDAPPMGAPMREFLAWVARYTVTHPGQVLAMALRAREGLLPSPVETRYRATGKLP
EEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCC
DRMTPARQKVLEVSRTVPNCPDLSRNDQLWSAVDLASAANVSSGVVKGLAEAGVLEAVEL
CCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEE
ETDPSFAIPDPDRKGAVLTGEQAVAAGELKAAIHAGGFSAFLLDGVTGSGKTEVYFEAIA
CCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
ETLKSDPEAQILVLLPEIALTQAILARFEARFGAAPAPWHSGLSPKDRRRTWREAVHGRA
HHHHCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCE
RIVIGARSALFLPYRKLRLIIVDEEHDTSFKQEDGVTYHARDLAVMRAKLEGGQVILASA
EEEEECCCCEECCCEEEEEEEEECCCCCCCHHHCCCEEEHHHHHHHHHCCCCCEEEEEEC
TPALETAVNAEAGRYVHLRLAARPGASRLPDIELVDLRTAPPGKGKWLSPVLERALMETL
CCHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
ANGEQSLLFLNRRGYAPLVICKACGERLKTPGTENWLTEHRYSGRLVCHVTGYSIPKPTK
CCCCCEEEEEECCCCCCCCEEHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECCCCCCCCC
CPQCGAPDSLMGVGPGVERVAEEVRTLLPHARIEIFSSDTAQGADVTRSLVERMERSEID
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCE
VMIGTQIVAKGHNFPNLTLVGVVDADSGLKGGDLRAGERTYQLLSQVAGRAGRAERPGRA
EEECCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHH
LVQTYAPDNPAMEALAANDRDGFLQIERDVRSELVLPPFGRLAAMILSAPSADLVDKAAL
HEEECCCCCHHHHHHHCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHH
DIAAAAPNGDGIEVFGPAPAPITVLRGRHRRRFLVKSPRIIDLSAYMAAWTARLRLPSQV
HEEEECCCCCCEEEECCCCCCCHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHCCCCCEE
RISIDIDPYSFL
EEEEECCCCCCC
>Mature Secondary Structure 
AVARILFTLPLPEPFDYAVPEGMTLQPGSYVAAPVGPHDRLGIVVDVLGDDVATGRKLK
CCEEEEEECCCCCCCCCCCCCCCEECCCCEEEECCCCCCCCCEEEEECCCCCCCCCEEE
TVTAVYDAPPMGAPMREFLAWVARYTVTHPGQVLAMALRAREGLLPSPVETRYRATGKLP
EEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCC
DRMTPARQKVLEVSRTVPNCPDLSRNDQLWSAVDLASAANVSSGVVKGLAEAGVLEAVEL
CCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEE
ETDPSFAIPDPDRKGAVLTGEQAVAAGELKAAIHAGGFSAFLLDGVTGSGKTEVYFEAIA
CCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
ETLKSDPEAQILVLLPEIALTQAILARFEARFGAAPAPWHSGLSPKDRRRTWREAVHGRA
HHHHCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCE
RIVIGARSALFLPYRKLRLIIVDEEHDTSFKQEDGVTYHARDLAVMRAKLEGGQVILASA
EEEEECCCCEECCCEEEEEEEEECCCCCCCHHHCCCEEEHHHHHHHHHCCCCCEEEEEEC
TPALETAVNAEAGRYVHLRLAARPGASRLPDIELVDLRTAPPGKGKWLSPVLERALMETL
CCHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
ANGEQSLLFLNRRGYAPLVICKACGERLKTPGTENWLTEHRYSGRLVCHVTGYSIPKPTK
CCCCCEEEEEECCCCCCCCEEHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECCCCCCCCC
CPQCGAPDSLMGVGPGVERVAEEVRTLLPHARIEIFSSDTAQGADVTRSLVERMERSEID
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCE
VMIGTQIVAKGHNFPNLTLVGVVDADSGLKGGDLRAGERTYQLLSQVAGRAGRAERPGRA
EEECCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHH
LVQTYAPDNPAMEALAANDRDGFLQIERDVRSELVLPPFGRLAAMILSAPSADLVDKAAL
HEEECCCCCHHHHHHHCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHH
DIAAAAPNGDGIEVFGPAPAPITVLRGRHRRRFLVKSPRIIDLSAYMAAWTARLRLPSQV
HEEEECCCCCCEEEECCCCCCCHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHCCCCCEE
RISIDIDPYSFL
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]