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The map label for this gene is pyrG

Identifier: 114332433

GI number: 114332433

Start: 2610982

End: 2612676

Strand: Reverse

Name: pyrG

Synonym: Neut_2483

Alternate gene names: 114332433

Gene position: 2612676-2610982 (Counterclockwise)

Preceding gene: 114332441

Following gene: 114332432

Centisome position: 98.18

GC content: 49.09

Gene sequence:

>1695_bases
ATGACTAAATTTGTCTTTGTGACTGGTGGCGTGGTGTCTTCCCTGGGCAAGGGAATTGCTGCCGCTTCTCTGGCAGCCTT
GCTTGAAACCCGTGGTATCCGGGTAACCATACTCAAGCTGGATCCTTATATCAATGTTGACCCCGGGACTATGAACCCGT
TTCAGCACGGAGAGGTATTTGTAACAGACGATGGTGCTGAAACCGATCTGGATCTGGGACATTACGAACGTTTCATTAGT
ACCAAAATGACCCGGCGCAATAATTTCACTACCGGGCAGATTTATGAAAGTGTAATTCGCAAGGAACGTCGCGGAGATTA
TCTTGGCGGGACTGTGCAGGTGATTCCGCACATTACGGATGAAATCAAACTGTTTATTCGCAATGGTGTTTCCGATGCAC
AAGTTGCAATTGTAGAAATTGGCGGCACAGTTGGCGACATTGAGTCTTTGCCGTTTCTGGAAGCAATCCGGCAAATGTCA
GTCCAGCTCCCGCATCACGATACCTGCTTCATTCACCTGACACTCCTGCCTTACATCAGCTCGGCCGGCGAACTTAAAAC
CAAACCTACTCAGCACTCGGTCAAGGAGCTCCGTGAAATCGGCATTCAGCCGGATGTGTTGCTTTGCCGCTCCGATCGCC
CGCTTCCACTGGACGAGCGGCGAAAAATCGCACTGTTTACCAATGTTCGCGAGGAATCGGTTATTTCCGCCATTGATGTG
GATAATATCTACAAAATTCCGGCACTATTACACGAACAAATGCTGGATGAAATTGTGTGTCACAGACTGGGTATCCTGGC
AAAACCAGCCAATCTGACAACCTGGAAAAAATTGATCCACGCACTGGAACATCCGGAACATGAAGTTTCTATAGCGTTAG
TCGGAAAATATGTTGATCTGACCGAATCGTATAAGTCGCTGTCGGAAGCATTGATCCATGCGGGGATCCATACACGCTGC
AAAATAAATATTCATTACATCGATTCGGAAAATATCGAACGACATGGAACCGGCTGCCTGGCAAACATGGATGCAATCCT
GGTGCCCGGGGGATTTGGTAAACGCGGGGTGGAAGGTAAAATCATGGCAATCAGCCATGCCCGCAATCATCAGATTCCTT
ATCTAGGCATCTGCCTCGGAATGCAGCTTGCAGTCATTGAATTTGCCCGCAATTGCCTGCAGCTTGAAAATGCTCACAGC
ACTGAATTCAACCCGGATACACCCTATCCTGTTCTCGGTTTGATCACTGAATGGCGTGATCGTCGCGGGCAAATCGAGAA
ACGTTCTCCCCAGACGGATCTGGGAGGAACCATGCGCCTGGGAGGGCAGGAATGTCTGCTGAAACCACACACCCTGGCGC
GCAAAATCTATGGAACAGATAAAATTATTGAGCGTCACCGGCATCGCTATGAGGTTAATGCCGAGTTTATTCCGCAGCTG
GAACAAGCCGGCCTGCAGGTAAGCGGCCTATCTGCTGCTGAAAATCTTTGTGAGATGATTGAGTTACCACAATCCAGACA
CCCTTGGTTTGTTGCTTGTCAGTTTCACCCGGAGTTCACCTCTACCCCCAGAAACGGCCACCCTCTATTTAACGACTATG
TCCGGGCTGCCATCAGTTTTGCCGATAAATCCGGCCACGCAAAACTTCGTGACCATAACATGCAGAAAAATGTCGCAACA
GACAGTACACACTGA

Upstream 100 bases:

>100_bases
CATGCCAGCAGACAACTTAACCTGTCAGCTGGAAATTTACCTTTGCCAGAGCTGGTGTGCGCATTGTTTCGATATATCAA
TACTGTTAAAATCACCTCCC

Downstream 100 bases:

>100_bases
TTCATCAAAGAAAATGGCTGCATGAATATTTTATTTTGACAACCCCTTTTATCTTAAATAGATAAGCAGCAATAACCTAT
CAACCTTATTCTCCAGTTGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MTKFVFVTGGVVSSLGKGIAAASLAALLETRGIRVTILKLDPYINVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERFIS
TKMTRRNNFTTGQIYESVIRKERRGDYLGGTVQVIPHITDEIKLFIRNGVSDAQVAIVEIGGTVGDIESLPFLEAIRQMS
VQLPHHDTCFIHLTLLPYISSAGELKTKPTQHSVKELREIGIQPDVLLCRSDRPLPLDERRKIALFTNVREESVISAIDV
DNIYKIPALLHEQMLDEIVCHRLGILAKPANLTTWKKLIHALEHPEHEVSIALVGKYVDLTESYKSLSEALIHAGIHTRC
KINIHYIDSENIERHGTGCLANMDAILVPGGFGKRGVEGKIMAISHARNHQIPYLGICLGMQLAVIEFARNCLQLENAHS
TEFNPDTPYPVLGLITEWRDRRGQIEKRSPQTDLGGTMRLGGQECLLKPHTLARKIYGTDKIIERHRHRYEVNAEFIPQL
EQAGLQVSGLSAAENLCEMIELPQSRHPWFVACQFHPEFTSTPRNGHPLFNDYVRAAISFADKSGHAKLRDHNMQKNVAT
DSTH

Sequences:

>Translated_564_residues
MTKFVFVTGGVVSSLGKGIAAASLAALLETRGIRVTILKLDPYINVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERFIS
TKMTRRNNFTTGQIYESVIRKERRGDYLGGTVQVIPHITDEIKLFIRNGVSDAQVAIVEIGGTVGDIESLPFLEAIRQMS
VQLPHHDTCFIHLTLLPYISSAGELKTKPTQHSVKELREIGIQPDVLLCRSDRPLPLDERRKIALFTNVREESVISAIDV
DNIYKIPALLHEQMLDEIVCHRLGILAKPANLTTWKKLIHALEHPEHEVSIALVGKYVDLTESYKSLSEALIHAGIHTRC
KINIHYIDSENIERHGTGCLANMDAILVPGGFGKRGVEGKIMAISHARNHQIPYLGICLGMQLAVIEFARNCLQLENAHS
TEFNPDTPYPVLGLITEWRDRRGQIEKRSPQTDLGGTMRLGGQECLLKPHTLARKIYGTDKIIERHRHRYEVNAEFIPQL
EQAGLQVSGLSAAENLCEMIELPQSRHPWFVACQFHPEFTSTPRNGHPLFNDYVRAAISFADKSGHAKLRDHNMQKNVAT
DSTH
>Mature_563_residues
TKFVFVTGGVVSSLGKGIAAASLAALLETRGIRVTILKLDPYINVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERFIST
KMTRRNNFTTGQIYESVIRKERRGDYLGGTVQVIPHITDEIKLFIRNGVSDAQVAIVEIGGTVGDIESLPFLEAIRQMSV
QLPHHDTCFIHLTLLPYISSAGELKTKPTQHSVKELREIGIQPDVLLCRSDRPLPLDERRKIALFTNVREESVISAIDVD
NIYKIPALLHEQMLDEIVCHRLGILAKPANLTTWKKLIHALEHPEHEVSIALVGKYVDLTESYKSLSEALIHAGIHTRCK
INIHYIDSENIERHGTGCLANMDAILVPGGFGKRGVEGKIMAISHARNHQIPYLGICLGMQLAVIEFARNCLQLENAHST
EFNPDTPYPVLGLITEWRDRRGQIEKRSPQTDLGGTMRLGGQECLLKPHTLARKIYGTDKIIERHRHRYEVNAEFIPQLE
QAGLQVSGLSAAENLCEMIELPQSRHPWFVACQFHPEFTSTPRNGHPLFNDYVRAAISFADKSGHAKLRDHNMQKNVATD
STH

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI28559085, Length=559, Percent_Identity=45.438282647585, Blast_Score=485, Evalue=1e-137,
Organism=Homo sapiens, GI28559083, Length=559, Percent_Identity=45.438282647585, Blast_Score=485, Evalue=1e-137,
Organism=Homo sapiens, GI221316689, Length=559, Percent_Identity=45.438282647585, Blast_Score=485, Evalue=1e-137,
Organism=Homo sapiens, GI148491070, Length=561, Percent_Identity=45.2762923351159, Blast_Score=476, Evalue=1e-134,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=63.2841328413284, Blast_Score=702, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=613, Percent_Identity=39.6411092985318, Blast_Score=423, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6322563, Length=555, Percent_Identity=41.8018018018018, Blast_Score=428, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319432, Length=555, Percent_Identity=40.5405405405405, Blast_Score=422, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=43.9285714285714, Blast_Score=454, Evalue=1e-127,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=42.970297029703, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_NITEC (Q0AD92)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748655.1
- ProteinModelPortal:   Q0AD92
- SMR:   Q0AD92
- STRING:   Q0AD92
- GeneID:   4273113
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2483
- NMPDR:   fig|335283.3.peg.1235
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- PhylomeDB:   Q0AD92
- ProtClustDB:   PRK05380
- BioCyc:   NEUT335283:NEUT_2483-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 63097; Mature: 62966

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 378-378 ACT_SITE 516-516 ACT_SITE 518-518

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFVFVTGGVVSSLGKGIAAASLAALLETRGIRVTILKLDPYINVDPGTMNPFQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFISTKMTRRNNFTTGQIYESVIRKERRGDYLGGTVQVIPHITD
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHH
EIKLFIRNGVSDAQVAIVEIGGTVGDIESLPFLEAIRQMSVQLPHHDTCFIHLTLLPYIS
HHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEHHHHC
SAGELKTKPTQHSVKELREIGIQPDVLLCRSDRPLPLDERRKIALFTNVREESVISAIDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHCCC
DNIYKIPALLHEQMLDEIVCHRLGILAKPANLTTWKKLIHALEHPEHEVSIALVGKYVDL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEHHHHH
TESYKSLSEALIHAGIHTRCKINIHYIDSENIERHGTGCLANMDAILVPGGFGKRGVEGK
HHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEECCCEEEECCCCCCCCCCCE
IMAISHARNHQIPYLGICLGMQLAVIEFARNCLQLENAHSTEFNPDTPYPVLGLITEWRD
EEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RRGQIEKRSPQTDLGGTMRLGGQECLLKPHTLARKIYGTDKIIERHRHRYEVNAEFIPQL
HCCCCCCCCCCCCCCCEEEECCHHHCCCHHHHHHHHHCCHHHHHHHHHHEECCHHHHHHH
EQAGLQVSGLSAAENLCEMIELPQSRHPWFVACQFHPEFTSTPRNGHPLFNDYVRAAISF
HHCCCEEECCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
ADKSGHAKLRDHNMQKNVATDSTH
HCCCCCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKFVFVTGGVVSSLGKGIAAASLAALLETRGIRVTILKLDPYINVDPGTMNPFQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFISTKMTRRNNFTTGQIYESVIRKERRGDYLGGTVQVIPHITD
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHH
EIKLFIRNGVSDAQVAIVEIGGTVGDIESLPFLEAIRQMSVQLPHHDTCFIHLTLLPYIS
HHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEHHHHC
SAGELKTKPTQHSVKELREIGIQPDVLLCRSDRPLPLDERRKIALFTNVREESVISAIDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHCCC
DNIYKIPALLHEQMLDEIVCHRLGILAKPANLTTWKKLIHALEHPEHEVSIALVGKYVDL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEHHHHH
TESYKSLSEALIHAGIHTRCKINIHYIDSENIERHGTGCLANMDAILVPGGFGKRGVEGK
HHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEECCCEEEECCCCCCCCCCCE
IMAISHARNHQIPYLGICLGMQLAVIEFARNCLQLENAHSTEFNPDTPYPVLGLITEWRD
EEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RRGQIEKRSPQTDLGGTMRLGGQECLLKPHTLARKIYGTDKIIERHRHRYEVNAEFIPQL
HCCCCCCCCCCCCCCCEEEECCHHHCCCHHHHHHHHHCCHHHHHHHHHHEECCHHHHHHH
EQAGLQVSGLSAAENLCEMIELPQSRHPWFVACQFHPEFTSTPRNGHPLFNDYVRAAISF
HHCCCEEECCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
ADKSGHAKLRDHNMQKNVATDSTH
HCCCCCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA