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The map label for this gene is eno

Identifier: 114332432

GI number: 114332432

Start: 2609407

End: 2610693

Strand: Reverse

Name: eno

Synonym: Neut_2482

Alternate gene names: 114332432

Gene position: 2610693-2609407 (Counterclockwise)

Preceding gene: 114332433

Following gene: 114332431

Centisome position: 98.11

GC content: 47.09

Gene sequence:

>1287_bases
ATGAGTGCAATCGTAGATGTAATCGCCCGTGAAATTATCGATTCTCGCGGCAACCCGACCATAGAGGCCGATGTATTGCT
AGAATCGGGTGTGCTCGGGCGTGCTTCAGTGCCATCCGGTGCATCTGTCGGGACACGAGAGGCTGTCGAGCTGCGTGATG
GCGATACACAACGCTATTATGGAAAAGGTGTACTAAAAGCGGTTGAAAACGTCAACGGTGAAATTTCTGAAGAAATCATG
GGGCTGGACGCCACAGAACAATGTTTCATTGATAAAACGTTAATTGAACTTGATGGCAGTGAAAATAAATCCAGACTTGG
TGCCAACGCGATCCTTGCCGTTTCACTCGCAGTAGCAAAGGCAGCAGCAGAAGAATCCAGCCTTCCCCTTTATCGTTATC
TGGGTGGGACTAACTCCAAGTGGTTGCCTGTTCCAATGATGAACCTGATTAATGGTGGTGTACACGCAAATAATCGCCTG
GATATGCAGGAATTTATGATTATACCGCTGGGTTTGCCAAGCCTGCGGGAAGCTGTGCGTTGTGGTGCAGAGGTATTCGG
CAAACTCAGAACATTGCTCCACAAAAAAAACTTGCCAACGACGGTTGGTGATGAAGGTGGATTTGCCCCTGGTTTCGCAC
ACAATGAAGAAGCATTGGGCTTGATTGTACAGGCCATAGACGAAGCGGGTTATCAGCCTGGTTCAGAGGTGGCAATCGGA
GTGGATTGTGCCAGTTCAGAATTTTTCCGGGATGGTAAATATCACCTTGCTGTAGACGGCCTCAGTCTTACTTCGGCTCA
ATTCATAGATTATCTGGCGTCCTGGGTGGAAAAATATCCGATCATCAGCATTGAAGATGGCATAAGCGAACAAGATTGGG
ACGGTTGGAAACTGCTGACTGAAAGACTTGGACAAACGGTTCAGCTAGTCGGTGATGATATATTTGCCACCAATACAAAA
ATTTTGAAGAAAGGTATCAATCGCAATATCGCCAACTCTATCCTGATCAAGATCAATCAGATTGGTACGCTTACTGAAAC
GCTGAATGCCATCGAAATGGCAAAGTGCGCAGGCTATACTGCCGTAGTCTCTCATCGTTCCGGCGAAACTGAGGATACAA
TGATTGCTGATATAGCAGTAGGCACTAACGCCTTACAGATCAAAACAGGCTCACTTTCGCGCTCGGATCGACTGGCCAAG
TACAACCAGCTGTTACGTATTGAGGAAGATTTGGGTGATGTCGCACAATACGCTGGAAGGTCAGCGTTTTACCAGTTAAA
ACCATGA

Upstream 100 bases:

>100_bases
GTACGTCATAATAACAATGCTCCCGGAACATATAGCAGCAAGGTAGTTTGTATATTCTAAAAATTCTCCCATCACTAATG
AATAACACAAGGAAAGCAGC

Downstream 100 bases:

>100_bases
AAATCACAACCGGACTATTGGTTCTTATGATTGCGTTGACGCAATACCCATTATGGTTCGGCAAGGGTGGCTGGTTGGAA
ATTATGGAAATGCATGAACA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSAIVDVIAREIIDSRGNPTIEADVLLESGVLGRASVPSGASVGTREAVELRDGDTQRYYGKGVLKAVENVNGEISEEIM
GLDATEQCFIDKTLIELDGSENKSRLGANAILAVSLAVAKAAAEESSLPLYRYLGGTNSKWLPVPMMNLINGGVHANNRL
DMQEFMIIPLGLPSLREAVRCGAEVFGKLRTLLHKKNLPTTVGDEGGFAPGFAHNEEALGLIVQAIDEAGYQPGSEVAIG
VDCASSEFFRDGKYHLAVDGLSLTSAQFIDYLASWVEKYPIISIEDGISEQDWDGWKLLTERLGQTVQLVGDDIFATNTK
ILKKGINRNIANSILIKINQIGTLTETLNAIEMAKCAGYTAVVSHRSGETEDTMIADIAVGTNALQIKTGSLSRSDRLAK
YNQLLRIEEDLGDVAQYAGRSAFYQLKP

Sequences:

>Translated_428_residues
MSAIVDVIAREIIDSRGNPTIEADVLLESGVLGRASVPSGASVGTREAVELRDGDTQRYYGKGVLKAVENVNGEISEEIM
GLDATEQCFIDKTLIELDGSENKSRLGANAILAVSLAVAKAAAEESSLPLYRYLGGTNSKWLPVPMMNLINGGVHANNRL
DMQEFMIIPLGLPSLREAVRCGAEVFGKLRTLLHKKNLPTTVGDEGGFAPGFAHNEEALGLIVQAIDEAGYQPGSEVAIG
VDCASSEFFRDGKYHLAVDGLSLTSAQFIDYLASWVEKYPIISIEDGISEQDWDGWKLLTERLGQTVQLVGDDIFATNTK
ILKKGINRNIANSILIKINQIGTLTETLNAIEMAKCAGYTAVVSHRSGETEDTMIADIAVGTNALQIKTGSLSRSDRLAK
YNQLLRIEEDLGDVAQYAGRSAFYQLKP
>Mature_427_residues
SAIVDVIAREIIDSRGNPTIEADVLLESGVLGRASVPSGASVGTREAVELRDGDTQRYYGKGVLKAVENVNGEISEEIMG
LDATEQCFIDKTLIELDGSENKSRLGANAILAVSLAVAKAAAEESSLPLYRYLGGTNSKWLPVPMMNLINGGVHANNRLD
MQEFMIIPLGLPSLREAVRCGAEVFGKLRTLLHKKNLPTTVGDEGGFAPGFAHNEEALGLIVQAIDEAGYQPGSEVAIGV
DCASSEFFRDGKYHLAVDGLSLTSAQFIDYLASWVEKYPIISIEDGISEQDWDGWKLLTERLGQTVQLVGDDIFATNTKI
LKKGINRNIANSILIKINQIGTLTETLNAIEMAKCAGYTAVVSHRSGETEDTMIADIAVGTNALQIKTGSLSRSDRLAKY
NQLLRIEEDLGDVAQYAGRSAFYQLKP

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=51.9721577726218, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=51.0440835266821, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=50.4651162790698, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=50.4651162790698, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=45.5607476635514, Blast_Score=349, Evalue=3e-96,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.4880952380952, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.4880952380952, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.4880952380952, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=61.1764705882353, Blast_Score=515, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=51.0440835266821, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=51.0440835266821, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=46.3541666666667, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=48.512585812357, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=48.0369515011547, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=48.0369515011547, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=48.0369515011547, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=47.8260869565217, Blast_Score=358, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580918, Length=429, Percent_Identity=48.951048951049, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580916, Length=429, Percent_Identity=48.951048951049, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580920, Length=429, Percent_Identity=48.951048951049, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580914, Length=429, Percent_Identity=48.951048951049, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=48.951048951049, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=48.951048951049, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_NITEC (Q0AD93)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748654.1
- ProteinModelPortal:   Q0AD93
- SMR:   Q0AD93
- STRING:   Q0AD93
- GeneID:   4273112
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2482
- NMPDR:   fig|335283.3.peg.1236
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q0AD93
- ProtClustDB:   PRK00077
- BioCyc:   NEUT335283:NEUT_2482-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46197; Mature: 46066

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDVIAREIIDSRGNPTIEADVLLESGVLGRASVPSGASVGTREAVELRDGDTQRYY
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVENVNGEISEEIMGLDATEQCFIDKTLIELDGSENKSRLGANAILAVSLAVAK
HHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHEEECCCCCCHHHCCHHHHHHHHHHHH
AAAEESSLPLYRYLGGTNSKWLPVPMMNLINGGVHANNRLDMQEFMIIPLGLPSLREAVR
HHHHCCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
CGAEVFGKLRTLLHKKNLPTTVGDEGGFAPGFAHNEEALGLIVQAIDEAGYQPGSEVAIG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
VDCASSEFFRDGKYHLAVDGLSLTSAQFIDYLASWVEKYPIISIEDGISEQDWDGWKLLT
EECCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHH
ERLGQTVQLVGDDIFATNTKILKKGINRNIANSILIKINQIGTLTETLNAIEMAKCAGYT
HHHCCEEEHHCCCHHHCCHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
AVVSHRSGETEDTMIADIAVGTNALQIKTGSLSRSDRLAKYNQLLRIEEDLGDVAQYAGR
EEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SAFYQLKP
CCEEEECC
>Mature Secondary Structure 
SAIVDVIAREIIDSRGNPTIEADVLLESGVLGRASVPSGASVGTREAVELRDGDTQRYY
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVENVNGEISEEIMGLDATEQCFIDKTLIELDGSENKSRLGANAILAVSLAVAK
HHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHEEECCCCCCHHHCCHHHHHHHHHHHH
AAAEESSLPLYRYLGGTNSKWLPVPMMNLINGGVHANNRLDMQEFMIIPLGLPSLREAVR
HHHHCCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
CGAEVFGKLRTLLHKKNLPTTVGDEGGFAPGFAHNEEALGLIVQAIDEAGYQPGSEVAIG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
VDCASSEFFRDGKYHLAVDGLSLTSAQFIDYLASWVEKYPIISIEDGISEQDWDGWKLLT
EECCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHH
ERLGQTVQLVGDDIFATNTKILKKGINRNIANSILIKINQIGTLTETLNAIEMAKCAGYT
HHHCCEEEHHCCCHHHCCHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
AVVSHRSGETEDTMIADIAVGTNALQIKTGSLSRSDRLAKYNQLLRIEEDLGDVAQYAGR
EEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SAFYQLKP
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA