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The map label for this gene is mltD [H]

Identifier: 114332420

GI number: 114332420

Start: 2592911

End: 2594275

Strand: Direct

Name: mltD [H]

Synonym: Neut_2469

Alternate gene names: 114332420

Gene position: 2592911-2594275 (Clockwise)

Preceding gene: 114332419

Following gene: 114332422

Centisome position: 97.44

GC content: 48.79

Gene sequence:

>1365_bases
ATGAAGACAAAATCGAATACCTTACTGGCATTGATTGCCGCAATAGTATTAACGTTTTCTCCTGTTACGGCGCAAGCCGA
TAGATCTTTAGAAAAAAATAACGGTATTACCGTTCTGGAAACAAAGCCGTATCAGGCACCATTACACAAACAGCAAGATG
ATCTATGGATGCGCGTACGGGCTGGATTCTCTCTGACCAATACACAAAGCCAGGAAGTTCGACTCCACGAAAGTAATTTC
AGTAAAAACCAGCGGTTTATAGATCATGTCGTTGAACGCAGTCAGCGCTATCTGTTTTATATCATCGAAGAAGTGGAGCG
ACGACATATGCCGGCCGAGATTGCGTTACTGCCGATCATTGAAAGCGCTTTTGACCCGGACGCCTACTCCCACAGACATG
CAGCAGGATTGTGGCAATTTATCCCCTCGACAGCAAAAGCATTTGGGCTGGAACAAAACTGGTGGCATGACGAACGACGC
GATATTATCGCCGCCACTCAGGCGGCGCTGGATTACCTGCAGATGCTCCACAAAATGTTTGGTAACTGGAAGTTGGCGCT
GGCCGCATACAATTGGGGCCAGGGGTCTCTGAAAAAAGCGATTGACGAAAATCATGGTGGGAATAAATCAATCAGCTACC
AGCAGATCGACCTTCCCTCCGAAACCAGAAACTATGTCTCCAAGCTGATTGCCATCAGAAATATCATTGCCCATCCTAAC
CGTTACGGCATCAAGCTGAAACCCATCCCGAACCGGCCTTACTTCGAGCAAATTACAATCACCCAGCAGATCGACGTTCA
ACTGGCCGCCAGGCTTGCCGGAATTTCAGAAAATGAATTTAATGCCTTGAATCCGGCGTATCACCGGCCCGTCATCAAGG
CAGAAGGATCTCCACGCGCCTTGCTGTTGCCCATCACCAAGGTCAAAACTTTTGTAGATAATCTTGAAAATTATGATAAA
GCGCTGGTTTCGTGGCGTGTTTATCAGGCTCAGCCAGCAGATACCTTGCAGGATTTATCCAATCGGTATCGCATCCCGGT
TGCGAAACTGGCCGAGACCAATGGTATCTCCGAGCGAGCAGTGCTGAAAAAAGGGCAGACACTGCTGGTGCCACGTGACG
GCAACAGCATCAGGGAAAGAACCTATCTGGCGCATAACCTACTCAAGACATCAGCGCAGTCTCCCCGGCCAGAACACTTC
GTATACACCGTCAGAAAGGGAGATACACTTCAGGCGATTGCCAGACGCTACGGAATTGATATCAACACCGTTCGTTCGTG
GAACAAGGGAAGCGACCGGCTTTCTATTGGACAGAAATTGACGCTCAAGCTGACTGCACCCTTCAATAGCCCTTATTCCT
CCTGA

Upstream 100 bases:

>100_bases
ACGGGCCGGCTGGTTCACTTTAAAATTCAAAAATAGAGGTGACCAGCCTTTTTTATTTTCAGAAAAAAATTATGCGAGTG
AACCGTTCGTTTTATTCCGG

Downstream 100 bases:

>100_bases
ACAATCAGCAACAGCTGACCGGAACATACATAATCTCCCTGCTTGCAGCTGACTTGTTGCACGATCCCGCTGACAGGCGC
ACTCAGGGTGATTTCCATCT

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKTKSNTLLALIAAIVLTFSPVTAQADRSLEKNNGITVLETKPYQAPLHKQQDDLWMRVRAGFSLTNTQSQEVRLHESNF
SKNQRFIDHVVERSQRYLFYIIEEVERRHMPAEIALLPIIESAFDPDAYSHRHAAGLWQFIPSTAKAFGLEQNWWHDERR
DIIAATQAALDYLQMLHKMFGNWKLALAAYNWGQGSLKKAIDENHGGNKSISYQQIDLPSETRNYVSKLIAIRNIIAHPN
RYGIKLKPIPNRPYFEQITITQQIDVQLAARLAGISENEFNALNPAYHRPVIKAEGSPRALLLPITKVKTFVDNLENYDK
ALVSWRVYQAQPADTLQDLSNRYRIPVAKLAETNGISERAVLKKGQTLLVPRDGNSIRERTYLAHNLLKTSAQSPRPEHF
VYTVRKGDTLQAIARRYGIDINTVRSWNKGSDRLSIGQKLTLKLTAPFNSPYSS

Sequences:

>Translated_454_residues
MKTKSNTLLALIAAIVLTFSPVTAQADRSLEKNNGITVLETKPYQAPLHKQQDDLWMRVRAGFSLTNTQSQEVRLHESNF
SKNQRFIDHVVERSQRYLFYIIEEVERRHMPAEIALLPIIESAFDPDAYSHRHAAGLWQFIPSTAKAFGLEQNWWHDERR
DIIAATQAALDYLQMLHKMFGNWKLALAAYNWGQGSLKKAIDENHGGNKSISYQQIDLPSETRNYVSKLIAIRNIIAHPN
RYGIKLKPIPNRPYFEQITITQQIDVQLAARLAGISENEFNALNPAYHRPVIKAEGSPRALLLPITKVKTFVDNLENYDK
ALVSWRVYQAQPADTLQDLSNRYRIPVAKLAETNGISERAVLKKGQTLLVPRDGNSIRERTYLAHNLLKTSAQSPRPEHF
VYTVRKGDTLQAIARRYGIDINTVRSWNKGSDRLSIGQKLTLKLTAPFNSPYSS
>Mature_454_residues
MKTKSNTLLALIAAIVLTFSPVTAQADRSLEKNNGITVLETKPYQAPLHKQQDDLWMRVRAGFSLTNTQSQEVRLHESNF
SKNQRFIDHVVERSQRYLFYIIEEVERRHMPAEIALLPIIESAFDPDAYSHRHAAGLWQFIPSTAKAFGLEQNWWHDERR
DIIAATQAALDYLQMLHKMFGNWKLALAAYNWGQGSLKKAIDENHGGNKSISYQQIDLPSETRNYVSKLIAIRNIIAHPN
RYGIKLKPIPNRPYFEQITITQQIDVQLAARLAGISENEFNALNPAYHRPVIKAEGSPRALLLPITKVKTFVDNLENYDK
ALVSWRVYQAQPADTLQDLSNRYRIPVAKLAETNGISERAVLKKGQTLLVPRDGNSIRERTYLAHNLLKTSAQSPRPEHF
VYTVRKGDTLQAIARRYGIDINTVRSWNKGSDRLSIGQKLTLKLTAPFNSPYSS

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=401, Percent_Identity=32.9177057356608, Blast_Score=234, Evalue=7e-63,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 51736; Mature: 51736

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKSNTLLALIAAIVLTFSPVTAQADRSLEKNNGITVLETKPYQAPLHKQQDDLWMRVR
CCCCCHHHHHHHHHHHHHCCCCHHHHHHCHHHCCCEEEEECCCCCCCCCCCCCHHEEHEE
AGFSLTNTQSQEVRLHESNFSKNQRFIDHVVERSQRYLFYIIEEVERRHMPAEIALLPII
CCCEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHCCCHHHHHHHHH
ESAFDPDAYSHRHAAGLWQFIPSTAKAFGLEQNWWHDERRDIIAATQAALDYLQMLHKMF
HHCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GNWKLALAAYNWGQGSLKKAIDENHGGNKSISYQQIDLPSETRNYVSKLIAIRNIIAHPN
CCEEEEEEEEECCCHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCC
RYGIKLKPIPNRPYFEQITITQQIDVQLAARLAGISENEFNALNPAYHRPVIKAEGSPRA
CCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEECCCCCE
LLLPITKVKTFVDNLENYDKALVSWRVYQAQPADTLQDLSNRYRIPVAKLAETNGISERA
EEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCHHHHHHCCCCCHHH
VLKKGQTLLVPRDGNSIRERTYLAHNLLKTSAQSPRPEHFVYTVRKGDTLQAIARRYGID
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCC
INTVRSWNKGSDRLSIGQKLTLKLTAPFNSPYSS
CCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKTKSNTLLALIAAIVLTFSPVTAQADRSLEKNNGITVLETKPYQAPLHKQQDDLWMRVR
CCCCCHHHHHHHHHHHHHCCCCHHHHHHCHHHCCCEEEEECCCCCCCCCCCCCHHEEHEE
AGFSLTNTQSQEVRLHESNFSKNQRFIDHVVERSQRYLFYIIEEVERRHMPAEIALLPII
CCCEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHCCCHHHHHHHHH
ESAFDPDAYSHRHAAGLWQFIPSTAKAFGLEQNWWHDERRDIIAATQAALDYLQMLHKMF
HHCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GNWKLALAAYNWGQGSLKKAIDENHGGNKSISYQQIDLPSETRNYVSKLIAIRNIIAHPN
CCEEEEEEEEECCCHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCC
RYGIKLKPIPNRPYFEQITITQQIDVQLAARLAGISENEFNALNPAYHRPVIKAEGSPRA
CCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEECCCCCE
LLLPITKVKTFVDNLENYDKALVSWRVYQAQPADTLQDLSNRYRIPVAKLAETNGISERA
EEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCHHHHHHCCCCCHHH
VLKKGQTLLVPRDGNSIRERTYLAHNLLKTSAQSPRPEHFVYTVRKGDTLQAIARRYGID
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCC
INTVRSWNKGSDRLSIGQKLTLKLTAPFNSPYSS
CCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]