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The map label for this gene is speE [H]

Identifier: 114332415

GI number: 114332415

Start: 2583258

End: 2584217

Strand: Reverse

Name: speE [H]

Synonym: Neut_2464

Alternate gene names: 114332415

Gene position: 2584217-2583258 (Counterclockwise)

Preceding gene: 114332416

Following gene: 114332410

Centisome position: 97.11

GC content: 51.56

Gene sequence:

>960_bases
ATGGAAGAATCCGGCAATGCTTATAAAGCGCGTTATGTAGCAGAGCGTCTGAGCCCGGATTTTGGTTTTTATCTGCGTGC
AAGCCGACAACTGGCAAAGGTAAACTCAGCCTGGCAAAAAATTGAGATTTTTGAGAATCCACTGTTTGGCCGGGTGATGC
GCATCGATGGCTGTTTCATGACCTCGGAGCGTGATGAATTTTTTTATCATGAACCCATGGTGCATTTGCCTGCCATCAGC
CATGGTGCACCGCGCCACGCCCTGATTGTTGGTGGTGGCGATGGCGGCGCGGCAGAAGAGTTACTCAAATATCCCGGGGT
GCAGCGCCTGGTACTGGCTGAACTGGATGGTGAGGTGATCCGGATGGCGCGAGAATGGTTGCCAACTATTCATCGCGGGG
CATTTGATGATCCGCGCCTGGAGCTGCTCCTGGGCGATGCGCTGCCTTTTATTGAAAGTAGCGTTGAACGCTTCGATCAG
ATCATTCTCGACTTGACCGACCCGTTCGGGCCAGCAACCGATCTTTACACAGTTGAATTTTATCGTGCCTGCCAAAAGCT
GTTGAATCCCGGTGGGGTAATTTCGCTGCATATCGGATCGCCCATTCATTTGCAGATACCCATGGCAAGAATTGCCGCTT
CGTTACGGGAGGTGTTTGCCATCGTTCGGCCCTATTTGCAGTATGTCCCGCTCTACGGCACGTTATGGTGTATGGCCATG
GCTTCCGATACAACTGATCCTCTTCTTCTGAGCTCAGAAGAAGTTAATGCACGTCTAGATAAACAGGGATTGCAGGATTT
GCAGCTCTACAACGGAGCGACTCACCATGCCCTGCTCGCACAACCTAATTTTGTACGCGAGATCCTGCAGCGACCGGTTA
AATTAATTCACCGGGGTGAAATACTTGAAGGAATCAACGACCCCGAATCATTGCCTGGCGTTACCGTCATCCCGGACTGA

Upstream 100 bases:

>100_bases
ATTCATCCGACAATCGCCCCAAAGCACAGCAACTGTTCGACAGCATTCTGGAAGCCTTCAATCCGGCTGATCCGCATCTG
CACCGGGTTGAGCGTGCGTG

Downstream 100 bases:

>100_bases
ACATACTGGACGTGCTGTATATCGTTACCAGCGGCAGCAGCAAAATCATTTTTTGCTGTTTTTCGCAGGTGGAGTGTGCT
TCACCCTCTGCATAGCCGTC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MEESGNAYKARYVAERLSPDFGFYLRASRQLAKVNSAWQKIEIFENPLFGRVMRIDGCFMTSERDEFFYHEPMVHLPAIS
HGAPRHALIVGGGDGGAAEELLKYPGVQRLVLAELDGEVIRMAREWLPTIHRGAFDDPRLELLLGDALPFIESSVERFDQ
IILDLTDPFGPATDLYTVEFYRACQKLLNPGGVISLHIGSPIHLQIPMARIAASLREVFAIVRPYLQYVPLYGTLWCMAM
ASDTTDPLLLSSEEVNARLDKQGLQDLQLYNGATHHALLAQPNFVREILQRPVKLIHRGEILEGINDPESLPGVTVIPD

Sequences:

>Translated_319_residues
MEESGNAYKARYVAERLSPDFGFYLRASRQLAKVNSAWQKIEIFENPLFGRVMRIDGCFMTSERDEFFYHEPMVHLPAIS
HGAPRHALIVGGGDGGAAEELLKYPGVQRLVLAELDGEVIRMAREWLPTIHRGAFDDPRLELLLGDALPFIESSVERFDQ
IILDLTDPFGPATDLYTVEFYRACQKLLNPGGVISLHIGSPIHLQIPMARIAASLREVFAIVRPYLQYVPLYGTLWCMAM
ASDTTDPLLLSSEEVNARLDKQGLQDLQLYNGATHHALLAQPNFVREILQRPVKLIHRGEILEGINDPESLPGVTVIPD
>Mature_319_residues
MEESGNAYKARYVAERLSPDFGFYLRASRQLAKVNSAWQKIEIFENPLFGRVMRIDGCFMTSERDEFFYHEPMVHLPAIS
HGAPRHALIVGGGDGGAAEELLKYPGVQRLVLAELDGEVIRMAREWLPTIHRGAFDDPRLELLLGDALPFIESSVERFDQ
IILDLTDPFGPATDLYTVEFYRACQKLLNPGGVISLHIGSPIHLQIPMARIAASLREVFAIVRPYLQYVPLYGTLWCMAM
ASDTTDPLLLSSEEVNARLDKQGLQDLQLYNGATHHALLAQPNFVREILQRPVKLIHRGEILEGINDPESLPGVTVIPD

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=280, Percent_Identity=31.0714285714286, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1786312, Length=276, Percent_Identity=37.3188405797101, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI25154106, Length=280, Percent_Identity=33.9285714285714, Blast_Score=140, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI212645616, Length=237, Percent_Identity=32.4894514767932, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6323175, Length=289, Percent_Identity=30.1038062283737, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6325326, Length=287, Percent_Identity=30.6620209059233, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24645443, Length=293, Percent_Identity=30.3754266211604, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI21355941, Length=218, Percent_Identity=30.2752293577982, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 35759; Mature: 35759

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEESGNAYKARYVAERLSPDFGFYLRASRQLAKVNSAWQKIEIFENPLFGRVMRIDGCFM
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCEEE
TSERDEFFYHEPMVHLPAISHGAPRHALIVGGGDGGAAEELLKYPGVQRLVLAELDGEVI
ECCCCCCEECCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHEEHHHHCCHHHH
RMAREWLPTIHRGAFDDPRLELLLGDALPFIESSVERFDQIILDLTDPFGPATDLYTVEF
HHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
YRACQKLLNPGGVISLHIGSPIHLQIPMARIAASLREVFAIVRPYLQYVPLYGTLWCMAM
HHHHHHHCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASDTTDPLLLSSEEVNARLDKQGLQDLQLYNGATHHALLAQPNFVREILQRPVKLIHRGE
HCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
ILEGINDPESLPGVTVIPD
HHHCCCCHHHCCCCEECCC
>Mature Secondary Structure
MEESGNAYKARYVAERLSPDFGFYLRASRQLAKVNSAWQKIEIFENPLFGRVMRIDGCFM
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCEEE
TSERDEFFYHEPMVHLPAISHGAPRHALIVGGGDGGAAEELLKYPGVQRLVLAELDGEVI
ECCCCCCEECCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHEEHHHHCCHHHH
RMAREWLPTIHRGAFDDPRLELLLGDALPFIESSVERFDQIILDLTDPFGPATDLYTVEF
HHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
YRACQKLLNPGGVISLHIGSPIHLQIPMARIAASLREVFAIVRPYLQYVPLYGTLWCMAM
HHHHHHHCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASDTTDPLLLSSEEVNARLDKQGLQDLQLYNGATHHALLAQPNFVREILQRPVKLIHRGE
HCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
ILEGINDPESLPGVTVIPD
HHHCCCCHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]