Click here to switch to the map view.

The map label for this gene is pheS [H]

Identifier: 114332287

GI number: 114332287

Start: 2453545

End: 2454582

Strand: Reverse

Name: pheS [H]

Synonym: Neut_2327

Alternate gene names: 114332287

Gene position: 2454582-2453545 (Counterclockwise)

Preceding gene: 114332288

Following gene: 114332286

Centisome position: 92.24

GC content: 42.39

Gene sequence:

>1038_bases
GTGGATCATTTCATTGCCATGAATAATCTGGAAGATTTGGTCAGTACCGCAATAAAATTATTTGATCAGGCGGAAAGTGT
TGTTGACCTAGAGCAAATTAAGGCGCGATATCTGGGTAAAGCAGGAGAAGTTACACTTTTGCTGAAAAAACTCAGGGAAC
TGACCCCGGAAGAAAGACCGGTAATGGGAGAGCGAATTAATCAGGCGAAAAAGTTGTTGGAAGATGCTTTGACTGAACGC
CGAAATGTAATTCATGAGAAAAATATGTCGGCTCGTCTGGCTGAGGAAAGTTTGGATGTGTCTTTGCCTGGGCGTGGATT
CGGGTTAGGGGGGGAGCACCCGGTTACGCTAACACTAACTCGTATTGAGTCGCTGTTTCATTCGATTGGATTTGATATTG
CAACTGGCCCTGAAATCGAAACAGATTTCTATAATTTTACGGCACTAAATATTGCCGAGAATCATCCTGCGCGTGCTATG
CACGATACTTTCTATATTGAAGGCGGGAAGCTGTTGCGAACACATACATCGCCGGTGCAGATTCACTATATGCAAAATCA
CCAGCCACCTATAAAGATTATTGCTCCTGGACGGGTGTATCGCTGTGATTCTGATGTAACTCATACGCCAATGTTCCATC
AGGTTGAGGGGCTGTGGATAGATGAAAATGTCAGTTTCTCTGCATTAAAGGGCGTGCTGGTGGAATTTATGAGAAATTTC
TTTGAAAAGGATAATTTATCAGTAAGATTCCGTCCCTCTTTTTTCCCTTTTACCGAACCTTCTGCTGAAATGGACATTGC
ATGTGTAATGTGTAATGGTAAAGGTTGTCGAGTTTGCGGTGAAACTGGTTGGTTGGAGGTGCTTGGATGCGGCATGGTAC
ATCCTAATGTCATGAATCATGTTGGCTTGAATAGTGAAGAAAATATTGGATTTGCTTTCGGGCTGGGTGTCGAACGTTTA
ACTATGTTGCGTTATGGAGTTAATGATCTGCGTCTATTCTTTGAAAATGACTTACGTTTTCTGAAACAATTTAATTGA

Upstream 100 bases:

>100_bases
CAATCAAGTAAAAACTAGCCTGGCAAACTAAAAAACAGTTTGTATACAAATTTATCAGGGGAGGTCTGCACTATGTTAGC
TCGACCTCCCTTTTTTATGA

Downstream 100 bases:

>100_bases
TGCTCTAGACTTGTAGTTATGAAATTTCCCGGGAACTGGCTACGTAAATTGGTTGACTACCAATGCTCTGATGAAGAGCT
GGCACACAAGTTGACTATGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MDHFIAMNNLEDLVSTAIKLFDQAESVVDLEQIKARYLGKAGEVTLLLKKLRELTPEERPVMGERINQAKKLLEDALTER
RNVIHEKNMSARLAEESLDVSLPGRGFGLGGEHPVTLTLTRIESLFHSIGFDIATGPEIETDFYNFTALNIAENHPARAM
HDTFYIEGGKLLRTHTSPVQIHYMQNHQPPIKIIAPGRVYRCDSDVTHTPMFHQVEGLWIDENVSFSALKGVLVEFMRNF
FEKDNLSVRFRPSFFPFTEPSAEMDIACVMCNGKGCRVCGETGWLEVLGCGMVHPNVMNHVGLNSEENIGFAFGLGVERL
TMLRYGVNDLRLFFENDLRFLKQFN

Sequences:

>Translated_345_residues
MDHFIAMNNLEDLVSTAIKLFDQAESVVDLEQIKARYLGKAGEVTLLLKKLRELTPEERPVMGERINQAKKLLEDALTER
RNVIHEKNMSARLAEESLDVSLPGRGFGLGGEHPVTLTLTRIESLFHSIGFDIATGPEIETDFYNFTALNIAENHPARAM
HDTFYIEGGKLLRTHTSPVQIHYMQNHQPPIKIIAPGRVYRCDSDVTHTPMFHQVEGLWIDENVSFSALKGVLVEFMRNF
FEKDNLSVRFRPSFFPFTEPSAEMDIACVMCNGKGCRVCGETGWLEVLGCGMVHPNVMNHVGLNSEENIGFAFGLGVERL
TMLRYGVNDLRLFFENDLRFLKQFN
>Mature_345_residues
MDHFIAMNNLEDLVSTAIKLFDQAESVVDLEQIKARYLGKAGEVTLLLKKLRELTPEERPVMGERINQAKKLLEDALTER
RNVIHEKNMSARLAEESLDVSLPGRGFGLGGEHPVTLTLTRIESLFHSIGFDIATGPEIETDFYNFTALNIAENHPARAM
HDTFYIEGGKLLRTHTSPVQIHYMQNHQPPIKIIAPGRVYRCDSDVTHTPMFHQVEGLWIDENVSFSALKGVLVEFMRNF
FEKDNLSVRFRPSFFPFTEPSAEMDIACVMCNGKGCRVCGETGWLEVLGCGMVHPNVMNHVGLNSEENIGFAFGLGVERL
TMLRYGVNDLRLFFENDLRFLKQFN

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=286, Percent_Identity=33.2167832167832, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4758340, Length=267, Percent_Identity=31.0861423220974, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=59.1715976331361, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17508957, Length=271, Percent_Identity=30.9963099630996, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=271, Percent_Identity=30.9963099630996, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=250, Percent_Identity=33.2, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321087, Length=274, Percent_Identity=28.8321167883212, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=316, Percent_Identity=27.5316455696203, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18858079, Length=269, Percent_Identity=29.7397769516729, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17137424, Length=304, Percent_Identity=26.9736842105263, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39107; Mature: 39107

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHFIAMNNLEDLVSTAIKLFDQAESVVDLEQIKARYLGKAGEVTLLLKKLRELTPEERP
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
VMGERINQAKKLLEDALTERRNVIHEKNMSARLAEESLDVSLPGRGFGLGGEHPVTLTLT
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEECCCCCCCCCCCCCEEEEHH
RIESLFHSIGFDIATGPEIETDFYNFTALNIAENHPARAMHDTFYIEGGKLLRTHTSPVQ
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHCCEEEEECCEEEEECCCCEE
IHYMQNHQPPIKIIAPGRVYRCDSDVTHTPMFHQVEGLWIDENVSFSALKGVLVEFMRNF
EEEECCCCCCEEEECCCEEEECCCCCCCCCCHHHCCCEEEECCCCHHHHHHHHHHHHHHH
FEKDNLSVRFRPSFFPFTEPSAEMDIACVMCNGKGCRVCGETGWLEVLGCGMVHPNVMNH
HCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCHHHHCCCCCCCHHHHH
VGLNSEENIGFAFGLGVERLTMLRYGVNDLRLFFENDLRFLKQFN
CCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDHFIAMNNLEDLVSTAIKLFDQAESVVDLEQIKARYLGKAGEVTLLLKKLRELTPEERP
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
VMGERINQAKKLLEDALTERRNVIHEKNMSARLAEESLDVSLPGRGFGLGGEHPVTLTLT
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEECCCCCCCCCCCCCEEEEHH
RIESLFHSIGFDIATGPEIETDFYNFTALNIAENHPARAMHDTFYIEGGKLLRTHTSPVQ
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHCCEEEEECCEEEEECCCCEE
IHYMQNHQPPIKIIAPGRVYRCDSDVTHTPMFHQVEGLWIDENVSFSALKGVLVEFMRNF
EEEECCCCCCEEEECCCEEEECCCCCCCCCCHHHCCCEEEECCCCHHHHHHHHHHHHHHH
FEKDNLSVRFRPSFFPFTEPSAEMDIACVMCNGKGCRVCGETGWLEVLGCGMVHPNVMNH
HCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCHHHHCCCCCCCHHHHH
VGLNSEENIGFAFGLGVERLTMLRYGVNDLRLFFENDLRFLKQFN
CCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]