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The map label for this gene is ptsN [H]

Identifier: 114332265

GI number: 114332265

Start: 2430281

End: 2430751

Strand: Direct

Name: ptsN [H]

Synonym: Neut_2305

Alternate gene names: 114332265

Gene position: 2430281-2430751 (Clockwise)

Preceding gene: 114332264

Following gene: 114332266

Centisome position: 91.33

GC content: 47.98

Gene sequence:

>471_bases
ATGAATCTCATTACTCAATTATTGCCGGAATCCAACATTATTATTGATCTGGATGTGACCAGCAAGAAACGTGTATTTGA
GCAAGTTGGTCTGTTATTTGAAAATACCCTGCATATTGCGCGCAGCCAGGTTTTTGACAGCTTGTTCGCACGTGAGAAAC
TCGGATCAACCGGGCTTGGTCAGGGAGTCGCCATCCCGCATGGCCGGATCAAGGGACTGCGCGAAGCAGCTGCAGCGCTG
GTCAGAATGAAGGAAGCTATACCTTTCGACGCTCCAGACGGCCAACCTGTCAGTATTATCTGCATCTTGCTGGTGCCGGA
AAAAGCAACAGATCAGCACTTGATGATACTGAGCGAACTAGCACAAATGTTTAGTAATAAAAGCTTCCGCGAAAAACTGC
GCCATGGCCGGAGTGCTAAAGAAATTCATCAACTCATCTCCGAGTGGACGCCTGAACATGTCTCAGGTTAG

Upstream 100 bases:

>100_bases
CAGCAAGTTGGGCACATATATAGTGAGTGATATAAAAAAACAGTCCCGTTTTCAATAAGAAGAAAACACCGCCTAATTTC
CTAAAGTGGTTTGAATCTCC

Downstream 100 bases:

>100_bases
CGTCAAACAACTATTTGAAGAAAACAAGGAAAAATTATGTCTGCAATGGGGTGAACCGCCGCCTGCCATAGACAGGCGGC
TTGAGAATCACCTGATCGCC

Product: putative PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MNLITQLLPESNIIIDLDVTSKKRVFEQVGLLFENTLHIARSQVFDSLFAREKLGSTGLGQGVAIPHGRIKGLREAAAAL
VRMKEAIPFDAPDGQPVSIICILLVPEKATDQHLMILSELAQMFSNKSFREKLRHGRSAKEIHQLISEWTPEHVSG

Sequences:

>Translated_156_residues
MNLITQLLPESNIIIDLDVTSKKRVFEQVGLLFENTLHIARSQVFDSLFAREKLGSTGLGQGVAIPHGRIKGLREAAAAL
VRMKEAIPFDAPDGQPVSIICILLVPEKATDQHLMILSELAQMFSNKSFREKLRHGRSAKEIHQLISEWTPEHVSG
>Mature_156_residues
MNLITQLLPESNIIIDLDVTSKKRVFEQVGLLFENTLHIARSQVFDSLFAREKLGSTGLGQGVAIPHGRIKGLREAAAAL
VRMKEAIPFDAPDGQPVSIICILLVPEKATDQHLMILSELAQMFSNKSFREKLRHGRSAKEIHQLISEWTPEHVSG

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=136, Percent_Identity=40.4411764705882, Blast_Score=99, Evalue=9e-23,
Organism=Escherichia coli, GI2367327, Length=132, Percent_Identity=28.030303030303, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1786951, Length=102, Percent_Identity=28.4313725490196, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI1788726, Length=141, Percent_Identity=27.6595744680851, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17347; Mature: 17347

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLITQLLPESNIIIDLDVTSKKRVFEQVGLLFENTLHIARSQVFDSLFAREKLGSTGLG
CCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QGVAIPHGRIKGLREAAAALVRMKEAIPFDAPDGQPVSIICILLVPEKATDQHLMILSEL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHH
AQMFSNKSFREKLRHGRSAKEIHQLISEWTPEHVSG
HHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNLITQLLPESNIIIDLDVTSKKRVFEQVGLLFENTLHIARSQVFDSLFAREKLGSTGLG
CCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QGVAIPHGRIKGLREAAAALVRMKEAIPFDAPDGQPVSIICILLVPEKATDQHLMILSEL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHH
AQMFSNKSFREKLRHGRSAKEIHQLISEWTPEHVSG
HHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]