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The map label for this gene is panB

Identifier: 114332253

GI number: 114332253

Start: 2419203

End: 2420012

Strand: Reverse

Name: panB

Synonym: Neut_2293

Alternate gene names: 114332253

Gene position: 2420012-2419203 (Counterclockwise)

Preceding gene: 114332254

Following gene: 114332252

Centisome position: 90.94

GC content: 49.88

Gene sequence:

>810_bases
ATGGATCGGCCGGTTACAAAACGTATAACGGTTTTGACACTGCAGAATATTTACCGGGAGAAGGGGAAAATAGCGGCCCT
GACCTGTTATGACGCAACGTTTGCTGCGGTACTGGAAAACGCAGGAGTTGATATTCTGCTGGTGGGGGATTCGCTTGGAA
ACGTGATACAAGGTCAGGCCAGTACTTTGCCGGTTACACTGGATGAAATGATCTACCACGTTTGCTGTGTAGAGCGCGGT
ACTCACAGCGTATTTATCCTGGCTGACATGCCGTTTGGCACGTTCCAGGTGTCTCCACAGGAAGCATTCAGGAATGCAGT
TCGGTTAATGGCTGCCGGTGCGCAGATGGTTAAGGTTGAAGGGGGAAGGCATATGGCAGAAACCATAGAATTTTTGACCT
GTCGTGGCATTCCGGTATGTGCCCATATCGGGTTAATACCTCAGTCTGTCCACCAGTTAGGCGGCTACAAAATTCAAGGG
AAAACACCTGATGGTGCCAGACAATTGCTGGAGGATGCACTGCTATTGCAAAAGGCAGGGGCGGCCATGCTCGTGATGGA
GTTGATCCCGGCTGCACTGGGAGAGGAAATTACCCGGTCACTATCCATCCCGACTATCGGCATTGGTGCTGGTGCAGTAT
GTTCGGGACAGGTCTTGGTGTTGCACGATATGTTAGGCATCAGTAGTGGTACTTTGCCGCGTTTTGTCAAAAATTTCATG
GCAGGCGCAGATAGTATTCAGGTGGCAGTCAGGAATTATGTGAAAGCAGTCAAAACCGGAGAATTTCCTGCGCATGAACA
TATGTTCTGA

Upstream 100 bases:

>100_bases
TGATCTGGCGAACAGTATTGCCGATTTTGATCTGTTACTGACGCGCATTGACCAGATGCGCAGTGCACGTGAATATTTTA
ATGTTGGAGCCAGTTAACAG

Downstream 100 bases:

>100_bases
CAGGTACGATACGAATTATTCACGTGGAAATAATCACTGATATCGCATCGTTGCGTGTGCGGCTTCAAGATGAAGCTTCA
ATAGCCCTGGTGCCAACTAT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MDRPVTKRITVLTLQNIYREKGKIAALTCYDATFAAVLENAGVDILLVGDSLGNVIQGQASTLPVTLDEMIYHVCCVERG
THSVFILADMPFGTFQVSPQEAFRNAVRLMAAGAQMVKVEGGRHMAETIEFLTCRGIPVCAHIGLIPQSVHQLGGYKIQG
KTPDGARQLLEDALLLQKAGAAMLVMELIPAALGEEITRSLSIPTIGIGAGAVCSGQVLVLHDMLGISSGTLPRFVKNFM
AGADSIQVAVRNYVKAVKTGEFPAHEHMF

Sequences:

>Translated_269_residues
MDRPVTKRITVLTLQNIYREKGKIAALTCYDATFAAVLENAGVDILLVGDSLGNVIQGQASTLPVTLDEMIYHVCCVERG
THSVFILADMPFGTFQVSPQEAFRNAVRLMAAGAQMVKVEGGRHMAETIEFLTCRGIPVCAHIGLIPQSVHQLGGYKIQG
KTPDGARQLLEDALLLQKAGAAMLVMELIPAALGEEITRSLSIPTIGIGAGAVCSGQVLVLHDMLGISSGTLPRFVKNFM
AGADSIQVAVRNYVKAVKTGEFPAHEHMF
>Mature_269_residues
MDRPVTKRITVLTLQNIYREKGKIAALTCYDATFAAVLENAGVDILLVGDSLGNVIQGQASTLPVTLDEMIYHVCCVERG
THSVFILADMPFGTFQVSPQEAFRNAVRLMAAGAQMVKVEGGRHMAETIEFLTCRGIPVCAHIGLIPQSVHQLGGYKIQG
KTPDGARQLLEDALLLQKAGAAMLVMELIPAALGEEITRSLSIPTIGIGAGAVCSGQVLVLHDMLGISSGTLPRFVKNFM
AGADSIQVAVRNYVKAVKTGEFPAHEHMF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=263, Percent_Identity=48.2889733840304, Blast_Score=271, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6319653, Length=276, Percent_Identity=39.8550724637681, Blast_Score=192, Evalue=3e-50,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_NITEC (Q0ADS2)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748475.1
- ProteinModelPortal:   Q0ADS2
- SMR:   Q0ADS2
- STRING:   Q0ADS2
- GeneID:   4274316
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2293
- NMPDR:   fig|335283.3.peg.30
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   Q0ADS2
- ProtClustDB:   PRK00311
- BioCyc:   NEUT335283:NEUT_2293-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28802; Mature: 28802

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: ACT_SITE 187-187 BINDING 89-89 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRPVTKRITVLTLQNIYREKGKIAALTCYDATFAAVLENAGVDILLVGDSLGNVIQGQA
CCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCEEEEECCCCCHHHCCCC
STLPVTLDEMIYHVCCVERGTHSVFILADMPFGTFQVSPQEAFRNAVRLMAAGAQMVKVE
CCCCEEHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCEEEEEC
GGRHMAETIEFLTCRGIPVCAHIGLIPQSVHQLGGYKIQGKTPDGARQLLEDALLLQKAG
CCCHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCC
AAMLVMELIPAALGEEITRSLSIPTIGIGAGAVCSGQVLVLHDMLGISSGTLPRFVKNFM
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHH
AGADSIQVAVRNYVKAVKTGEFPAHEHMF
CCCHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDRPVTKRITVLTLQNIYREKGKIAALTCYDATFAAVLENAGVDILLVGDSLGNVIQGQA
CCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCEEEEECCCCCHHHCCCC
STLPVTLDEMIYHVCCVERGTHSVFILADMPFGTFQVSPQEAFRNAVRLMAAGAQMVKVE
CCCCEEHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCEEEEEC
GGRHMAETIEFLTCRGIPVCAHIGLIPQSVHQLGGYKIQGKTPDGARQLLEDALLLQKAG
CCCHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCC
AAMLVMELIPAALGEEITRSLSIPTIGIGAGAVCSGQVLVLHDMLGISSGTLPRFVKNFM
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHH
AGADSIQVAVRNYVKAVKTGEFPAHEHMF
CCCHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA