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The map label for this gene is bioD [H]

Identifier: 114332099

GI number: 114332099

Start: 2263602

End: 2264285

Strand: Reverse

Name: bioD [H]

Synonym: Neut_2134

Alternate gene names: 114332099

Gene position: 2264285-2263602 (Counterclockwise)

Preceding gene: 114332100

Following gene: 114332097

Centisome position: 85.09

GC content: 49.85

Gene sequence:

>684_bases
ATGGCAACAGGATTCTTTGTAACGGGTACGGATACCGGAATTGGTAAAACTACAGTGAGTTGTGCGCTGTTGCATGCGTT
TTCGACGAATGGCTATACCGCTGCCGGAATGAAGCCAGTGGTGTCAGGTCGTAAAGATGGATTATGGCAGGATGTGGAAA
ATCTGCAGGCAGCCAGCACGGTTAGCATGCCACGTAAACTGATTAATCCTTATGCCTTTGTCGAGTCAATAGCACCGCAT
ATCGCTGCACAGCAGACGAATATCCGTATCGATCTGGAAGTGATTCGGCAGGCTTGCGAGAAGCTGCAAACAGCAGCAGA
GATAACGATTGTCGAAGGTGTCGGTGGTTTCATGGTGCCGCTGTCGCTATCAGGTTTGTCAGGTGATCGATACGACACGC
GTGATCTTGCCTGTGTGTTAGGGCTGCCAGTAATCCTGGTTGTGGGAATGCGGCTAGGTTGTCTTAACCATGCATTACTG
ACAGCTCGCGCAGTTGAATCAGCCGGTTTGAAATTGGCAGGCTGGGTGGCAAACAGAATTGATCCGGATATGTTGCAGCC
GGAAGCAAACCTGCAAACACTGGTCGAATGGCTGGATTGCCCATTATTGGGTATCTTGCCGTATCAAGCGCAACCTGACG
TGCAGCAACTTGCGCAATATCTTGATCTTTCCAGATTACGGTAA

Upstream 100 bases:

>100_bases
CAACCTACGAAGTAGTTTATGGTCACGCCTGGAAACCGGAGATACGTAAAGTTCAGCTAAAGCCGGAGACGCGGCGCAAG
CTGGGGCTGGATCCGTAATA

Downstream 100 bases:

>100_bases
CCGGGTGATTCAGGCCTGATTACAGTATCTGCCTGAATTTCTCGGTAGCCTTGATGAGTTCAGCCTGAATCCCAAGTTCC
CAGGCGGAATGGCCGGCATC

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MATGFFVTGTDTGIGKTTVSCALLHAFSTNGYTAAGMKPVVSGRKDGLWQDVENLQAASTVSMPRKLINPYAFVESIAPH
IAAQQTNIRIDLEVIRQACEKLQTAAEITIVEGVGGFMVPLSLSGLSGDRYDTRDLACVLGLPVILVVGMRLGCLNHALL
TARAVESAGLKLAGWVANRIDPDMLQPEANLQTLVEWLDCPLLGILPYQAQPDVQQLAQYLDLSRLR

Sequences:

>Translated_227_residues
MATGFFVTGTDTGIGKTTVSCALLHAFSTNGYTAAGMKPVVSGRKDGLWQDVENLQAASTVSMPRKLINPYAFVESIAPH
IAAQQTNIRIDLEVIRQACEKLQTAAEITIVEGVGGFMVPLSLSGLSGDRYDTRDLACVLGLPVILVVGMRLGCLNHALL
TARAVESAGLKLAGWVANRIDPDMLQPEANLQTLVEWLDCPLLGILPYQAQPDVQQLAQYLDLSRLR
>Mature_226_residues
ATGFFVTGTDTGIGKTTVSCALLHAFSTNGYTAAGMKPVVSGRKDGLWQDVENLQAASTVSMPRKLINPYAFVESIAPHI
AAQQTNIRIDLEVIRQACEKLQTAAEITIVEGVGGFMVPLSLSGLSGDRYDTRDLACVLGLPVILVVGMRLGCLNHALLT
ARAVESAGLKLAGWVANRIDPDMLQPEANLQTLVEWLDCPLLGILPYQAQPDVQQLAQYLDLSRLR

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=231, Percent_Identity=41.1255411255411, Blast_Score=164, Evalue=3e-42,
Organism=Escherichia coli, GI87081944, Length=231, Percent_Identity=38.5281385281385, Blast_Score=144, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324385, Length=195, Percent_Identity=32.3076923076923, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 24420; Mature: 24288

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGFFVTGTDTGIGKTTVSCALLHAFSTNGYTAAGMKPVVSGRKDGLWQDVENLQAAST
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEECCCCCHHHCCCCCCHHHHHHHHHHHHH
VSMPRKLINPYAFVESIAPHIAAQQTNIRIDLEVIRQACEKLQTAAEITIVEGVGGFMVP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHEEEEECCCCEEEE
LSLSGLSGDRYDTRDLACVLGLPVILVVGMRLGCLNHALLTARAVESAGLKLAGWVANRI
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCC
DPDMLQPEANLQTLVEWLDCPLLGILPYQAQPDVQQLAQYLDLSRLR
CHHHCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATGFFVTGTDTGIGKTTVSCALLHAFSTNGYTAAGMKPVVSGRKDGLWQDVENLQAAST
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCEECCCCCHHHCCCCCCHHHHHHHHHHHHH
VSMPRKLINPYAFVESIAPHIAAQQTNIRIDLEVIRQACEKLQTAAEITIVEGVGGFMVP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHEEEEECCCCEEEE
LSLSGLSGDRYDTRDLACVLGLPVILVVGMRLGCLNHALLTARAVESAGLKLAGWVANRI
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCC
DPDMLQPEANLQTLVEWLDCPLLGILPYQAQPDVQQLAQYLDLSRLR
CHHHCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA