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The map label for this gene is trpE [H]

Identifier: 114332063

GI number: 114332063

Start: 2221654

End: 2223141

Strand: Direct

Name: trpE [H]

Synonym: Neut_2098

Alternate gene names: 114332063

Gene position: 2221654-2223141 (Clockwise)

Preceding gene: 114332062

Following gene: 114332066

Centisome position: 83.49

GC content: 46.91

Gene sequence:

>1488_bases
ATGGATCATTGCATATCTGAAAGTGAATTCAACGATCTGAAAGCACAAGGGTATAACCGCATACCTTTGGTGCTGGAGAC
ATTCGCCGATCTCGACACTCCCCTTTCCATTTATCTCAAACTGGCCAATCAGCCCTATTCTTACCTGCTGGAATCGATAC
TGGGAGGAGAACGGTTCGGACGCTATTCGATTATTGGATTACCCGCAACAACCAGGCTAGAAGCTTGCTCCAACCATATT
CGTATAATCAGTAACGATACTATAGAAGAAAAAATCGACACAACCGATGTACTTGGTTTTATTGACTCTTTCCTAAATCG
GTTCAAAGTTGCTCCTTACGCCAAGTTACCTCGTTTTGGCGGAGGTCTGGCTGGCTATTTTTCCTATGACACCGTTCGTT
ACATTGAACCCAGGCTGTCTGGTCGTGCCCGTCCAGATACGTTACAGACACCGGATATTTTGCTACTGCTCTCGGAAGAA
CTGATCGTTATGGACAATCTTTCCGGCAAACTTTATCTGATTATCTACATCAATCCAACATTGGAAAATGCTTATCAGAC
CGGGAAAGATCATCTCAAGAAATTATTACATAAACTGCGGGAGCCTCTGCCAGTACCAGTCGAAAAACCAACCTCTTCCG
GAATAGCTGTTAATGAGTTTTCCGAAGCTGACTTTATTGCCGTTGTGGAAAAGGCTAAACGCTATATTGTCGAAGGCGAC
ATCATGCAGGTTGTCCTCTCACAACGCACTAGTAAACCCTATTCCGCTTCACCACTCGCATTGTATCGTGCCTTGCGTAG
CCTTAATCCATCACCCTACATGTTCAATTATCATTTAGGTGACTTTCACGTCGTGGGGGCCTCACCAGAAATTCTGGTCA
GACTGGAAAAGGATACGGTTACCGTCCGCCCGATTGCCGGCACTCGGCCACGTGGATTGAATATTCAGGCAGATCAGGCG
CTGGCAGTAGATTTACTGGCAGACCCCAAAGAACGAGCCGAACACATCATGCTGATGGATCTGGGGCGCAACGATATCGG
CCGGGTTGCACGGACAGGCAGTGTCAAGGTCACTGAAAACATGCAAATAGAATATTATTCGCACGTTATGCATATTGTTT
CCAATGTCGAAGGGAAACTCAAACCGGATCTGAACGCCATTGATGTATTACGCGCCACTTTCCCGGCAGGCACTGTTAGT
GGGGCACCTAAGGTCAGAGCAATGGAAATCATCGACGAGCTGGAAATCTCAAAACGTGGAATCTATGCCGGTGCAGTTGG
TTATCTCGGATTCAACGGTGATATGGATCTGGCTATCACTATCCGTACCGGAATCATCAAGGATGGTATGTTACATGTGC
AGGCAGGTGCAGGCATTGTCGCCGATTCCATTCCGCAGAATGAGTGGATTGAAACATGCAACAAGGCCAAAGCATTATTA
CGCGCAGCAGAGATTGCCGAAAACGGACTGGACAGTGCAATTACCTGA

Upstream 100 bases:

>100_bases
AAACACATAAAGCTATACCGGCTTGTCACCGTGATAAACATGCAACAGCCGGTATAACCTCATTCCATCACTTTACCATT
TTAGTAACACTCTGCCTTCC

Downstream 100 bases:

>100_bases
ATATTCTCTTACCTGCTGGCAAAGAAATTTTACTTCAAGACGGATTGGAACGAGTACCTACCCCGTATTCGTAAACAAGC
TTGCCTCCCAGCCAACCAGC

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MDHCISESEFNDLKAQGYNRIPLVLETFADLDTPLSIYLKLANQPYSYLLESILGGERFGRYSIIGLPATTRLEACSNHI
RIISNDTIEEKIDTTDVLGFIDSFLNRFKVAPYAKLPRFGGGLAGYFSYDTVRYIEPRLSGRARPDTLQTPDILLLLSEE
LIVMDNLSGKLYLIIYINPTLENAYQTGKDHLKKLLHKLREPLPVPVEKPTSSGIAVNEFSEADFIAVVEKAKRYIVEGD
IMQVVLSQRTSKPYSASPLALYRALRSLNPSPYMFNYHLGDFHVVGASPEILVRLEKDTVTVRPIAGTRPRGLNIQADQA
LAVDLLADPKERAEHIMLMDLGRNDIGRVARTGSVKVTENMQIEYYSHVMHIVSNVEGKLKPDLNAIDVLRATFPAGTVS
GAPKVRAMEIIDELEISKRGIYAGAVGYLGFNGDMDLAITIRTGIIKDGMLHVQAGAGIVADSIPQNEWIETCNKAKALL
RAAEIAENGLDSAIT

Sequences:

>Translated_495_residues
MDHCISESEFNDLKAQGYNRIPLVLETFADLDTPLSIYLKLANQPYSYLLESILGGERFGRYSIIGLPATTRLEACSNHI
RIISNDTIEEKIDTTDVLGFIDSFLNRFKVAPYAKLPRFGGGLAGYFSYDTVRYIEPRLSGRARPDTLQTPDILLLLSEE
LIVMDNLSGKLYLIIYINPTLENAYQTGKDHLKKLLHKLREPLPVPVEKPTSSGIAVNEFSEADFIAVVEKAKRYIVEGD
IMQVVLSQRTSKPYSASPLALYRALRSLNPSPYMFNYHLGDFHVVGASPEILVRLEKDTVTVRPIAGTRPRGLNIQADQA
LAVDLLADPKERAEHIMLMDLGRNDIGRVARTGSVKVTENMQIEYYSHVMHIVSNVEGKLKPDLNAIDVLRATFPAGTVS
GAPKVRAMEIIDELEISKRGIYAGAVGYLGFNGDMDLAITIRTGIIKDGMLHVQAGAGIVADSIPQNEWIETCNKAKALL
RAAEIAENGLDSAIT
>Mature_495_residues
MDHCISESEFNDLKAQGYNRIPLVLETFADLDTPLSIYLKLANQPYSYLLESILGGERFGRYSIIGLPATTRLEACSNHI
RIISNDTIEEKIDTTDVLGFIDSFLNRFKVAPYAKLPRFGGGLAGYFSYDTVRYIEPRLSGRARPDTLQTPDILLLLSEE
LIVMDNLSGKLYLIIYINPTLENAYQTGKDHLKKLLHKLREPLPVPVEKPTSSGIAVNEFSEADFIAVVEKAKRYIVEGD
IMQVVLSQRTSKPYSASPLALYRALRSLNPSPYMFNYHLGDFHVVGASPEILVRLEKDTVTVRPIAGTRPRGLNIQADQA
LAVDLLADPKERAEHIMLMDLGRNDIGRVARTGSVKVTENMQIEYYSHVMHIVSNVEGKLKPDLNAIDVLRATFPAGTVS
GAPKVRAMEIIDELEISKRGIYAGAVGYLGFNGDMDLAITIRTGIIKDGMLHVQAGAGIVADSIPQNEWIETCNKAKALL
RAAEIAENGLDSAIT

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=413, Percent_Identity=39.7094430992736, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1788114, Length=465, Percent_Identity=31.8279569892473, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1786809, Length=221, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082077, Length=261, Percent_Identity=29.1187739463602, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=485, Percent_Identity=41.0309278350515, Blast_Score=318, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6324361, Length=374, Percent_Identity=28.0748663101604, Blast_Score=134, Evalue=3e-32,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 54735; Mature: 54735

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHCISESEFNDLKAQGYNRIPLVLETFADLDTPLSIYLKLANQPYSYLLESILGGERFG
CCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCC
RYSIIGLPATTRLEACSNHIRIISNDTIEEKIDTTDVLGFIDSFLNRFKVAPYAKLPRFG
CEEEEECCCHHHHHHHCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHCCCCCHHCCCCC
GGLAGYFSYDTVRYIEPRLSGRARPDTLQTPDILLLLSEELIVMDNLSGKLYLIIYINPT
CCEEEEECCCEEEEECHHHCCCCCCCCCCCCCEEEEECCCEEEEECCCCCEEEEEEECCC
LENAYQTGKDHLKKLLHKLREPLPVPVEKPTSSGIAVNEFSEADFIAVVEKAKRYIVEGD
HHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCHH
IMQVVLSQRTSKPYSASPLALYRALRSLNPSPYMFNYHLGDFHVVGASPEILVRLEKDTV
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEEEEECCEE
TVRPIAGTRPRGLNIQADQALAVDLLADPKERAEHIMLMDLGRNDIGRVARTGSVKVTEN
EEEECCCCCCCCCCEECCCEEEEEECCCCHHHHCEEEEEECCCCCHHHHHCCCCEEEECC
MQIEYYSHVMHIVSNVEGKLKPDLNAIDVLRATFPAGTVSGAPKVRAMEIIDELEISKRG
CCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
IYAGAVGYLGFNGDMDLAITIRTGIIKDGMLHVQAGAGIVADSIPQNEWIETCNKAKALL
EEEEEEEEEEECCCCEEEEEEEECCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHH
RAAEIAENGLDSAIT
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MDHCISESEFNDLKAQGYNRIPLVLETFADLDTPLSIYLKLANQPYSYLLESILGGERFG
CCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCC
RYSIIGLPATTRLEACSNHIRIISNDTIEEKIDTTDVLGFIDSFLNRFKVAPYAKLPRFG
CEEEEECCCHHHHHHHCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHCCCCCHHCCCCC
GGLAGYFSYDTVRYIEPRLSGRARPDTLQTPDILLLLSEELIVMDNLSGKLYLIIYINPT
CCEEEEECCCEEEEECHHHCCCCCCCCCCCCCEEEEECCCEEEEECCCCCEEEEEEECCC
LENAYQTGKDHLKKLLHKLREPLPVPVEKPTSSGIAVNEFSEADFIAVVEKAKRYIVEGD
HHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCHH
IMQVVLSQRTSKPYSASPLALYRALRSLNPSPYMFNYHLGDFHVVGASPEILVRLEKDTV
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEEEEECCEE
TVRPIAGTRPRGLNIQADQALAVDLLADPKERAEHIMLMDLGRNDIGRVARTGSVKVTEN
EEEECCCCCCCCCCEECCCEEEEEECCCCHHHHCEEEEEECCCCCHHHHHCCCCEEEECC
MQIEYYSHVMHIVSNVEGKLKPDLNAIDVLRATFPAGTVSGAPKVRAMEIIDELEISKRG
CCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
IYAGAVGYLGFNGDMDLAITIRTGIIKDGMLHVQAGAGIVADSIPQNEWIETCNKAKALL
EEEEEEEEEEECCCCEEEEEEEECCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHH
RAAEIAENGLDSAIT
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10417653 [H]