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The map label for this gene is nadE [H]

Identifier: 114331974

GI number: 114331974

Start: 2121952

End: 2123559

Strand: Direct

Name: nadE [H]

Synonym: Neut_2008

Alternate gene names: 114331974

Gene position: 2121952-2123559 (Clockwise)

Preceding gene: 114331973

Following gene: 114331975

Centisome position: 79.74

GC content: 51.68

Gene sequence:

>1608_bases
ATGAAGATCGCGCTTGCCCAGATCAACTGCATGGCAGGCGATTTGAACGGTAACCCGCTTAAAATACTGCACGCCTGCCA
GCAAGCCAGGGAAGCCGGGGCCAGGCTGGTTATCACTCCGGAAATGGCGCTGTGTGGCTCCCTTCCGGAAGACTGGTTGC
TGCGCGCAGAGTTTGTACAAGTCTGTCATCAGGCTTTGATGGATCTAGCCACGCAGATACATGACGTAACATTGGTAATC
GGGCATCCGCACCGCACGAACGGTAATCTGCTCAATGCTGTTTCCGTTATTCGAGATGGTCGCCTGCTTATCACCTATGG
TAAAAATCATTTGCTCACCGATCCGTTGCTGGATGAACGCCGTTATTTCACTCCCGGCAACCAGCCTTGTACGTTTGAGT
GTGACGGTACACTTTTCGGATTGACCACTTTCTCTGATTATCAGCATCCGGCACACTTGCAGGCTTTGCGTTCTGCTGAC
GTGCAGGTTTTACTGACGGTGGGTGCATCACCCTACGCTATTGATGGTCAGGCTGACCGACTCCGAATTGTACGGGCGGG
AATTACGCAAACCGGTCTGCCAACTGTTTACACCAACCTAGTCGGCGGACAGGACGAACTGGTTTTCGATGGTGCTTCAT
TTGCGATGGATCACAGCGGTAAACTCGTTTGTCAGCTAGCGGCTTTCCAGGAAACACTTGGCTTGATTGAGCTTCATAAT
AATCAACTGATCCCCGGACAATGTTCTCCGCTGCCAGATCGGATCGAAAGTGTGTACTCGGCACTACGTCTGGGCTTACG
CGATTTTATTGATAAAAACAGAATCTCCGGAGTATTGATTGGTTTGTCAGGCGGGGTTGATTCAGCGCTGGTACTGGCGA
TTGCGGTGGATGCGCTTGGAGCAGATCGGGTCAGCAACGTGATGATGCCCTCCCCTTATACCGCCGACATCAGCCTGGAA
GATGCGCAAATCATGGCAGGCAATCTGGGTGTACACTACACTGAAATACCCATTACCGAGCTATTCGAGCAATTCAAACA
AACATTACACCCCGCACTGCAAACCTATCCAGCTACCGGTCCCCAAACTACCATGGAGAACCTGCAGGCCCGTATCCGTG
GAACCTTGTTGATGGCATTAGCCAATCAATCCGGAAGACTGGTACTGACAACAAGTAACAAATCCGAAACTGCAGTGGGC
TACAGCACGCTATACGGCGATATGGCTGGTGGGTTTTCGATACTCAAGGATGTCAGTAAAACGCTGGTTTATCAGCTTTG
CCACTACCGTAACAGAATTTCACCCATCATCCCGGAGCGCATCCTGCAGCGCCCGCCTTCGGCTGAATTACGTCCCGATC
AGACTGATCAGGATAATCTCCCACCGTATGCTGTACTGGATGCAATCATTGCAGCCTACGTGGAAAACAACCTGTCACCT
GCAGAAATTATTGCCATGAATTATCCGGAGGAAACCGTCCGGCAGGTGCTGCACATGATCCATTCCTGCGAGTACAAACG
CAGACAGGCAGCTCCCGGCATCCGCATTACCCGACGTGATTTCGGTCCATCATGGCGATTCCCGATCACGTCTGGTTTCC
GGCAATGA

Upstream 100 bases:

>100_bases
TATCCAGCAAGATTTTCGCCAAATCATTCTGCAAGCCAGGCCGGATGCAAATCTGCCTTTCTAACCGGGTTCCAGTTACG
GACCCGAATACCCTTCCACA

Downstream 100 bases:

>100_bases
TCAGCCCCAATAATATTTTGGCATTCCTCAATTCAACAGTTGTTACGCAATTTCGTATACAACAGGTGTTATCGTACTTG
CGGCTGAAATCACAATCTGC

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MKIALAQINCMAGDLNGNPLKILHACQQAREAGARLVITPEMALCGSLPEDWLLRAEFVQVCHQALMDLATQIHDVTLVI
GHPHRTNGNLLNAVSVIRDGRLLITYGKNHLLTDPLLDERRYFTPGNQPCTFECDGTLFGLTTFSDYQHPAHLQALRSAD
VQVLLTVGASPYAIDGQADRLRIVRAGITQTGLPTVYTNLVGGQDELVFDGASFAMDHSGKLVCQLAAFQETLGLIELHN
NQLIPGQCSPLPDRIESVYSALRLGLRDFIDKNRISGVLIGLSGGVDSALVLAIAVDALGADRVSNVMMPSPYTADISLE
DAQIMAGNLGVHYTEIPITELFEQFKQTLHPALQTYPATGPQTTMENLQARIRGTLLMALANQSGRLVLTTSNKSETAVG
YSTLYGDMAGGFSILKDVSKTLVYQLCHYRNRISPIIPERILQRPPSAELRPDQTDQDNLPPYAVLDAIIAAYVENNLSP
AEIIAMNYPEETVRQVLHMIHSCEYKRRQAAPGIRITRRDFGPSWRFPITSGFRQ

Sequences:

>Translated_535_residues
MKIALAQINCMAGDLNGNPLKILHACQQAREAGARLVITPEMALCGSLPEDWLLRAEFVQVCHQALMDLATQIHDVTLVI
GHPHRTNGNLLNAVSVIRDGRLLITYGKNHLLTDPLLDERRYFTPGNQPCTFECDGTLFGLTTFSDYQHPAHLQALRSAD
VQVLLTVGASPYAIDGQADRLRIVRAGITQTGLPTVYTNLVGGQDELVFDGASFAMDHSGKLVCQLAAFQETLGLIELHN
NQLIPGQCSPLPDRIESVYSALRLGLRDFIDKNRISGVLIGLSGGVDSALVLAIAVDALGADRVSNVMMPSPYTADISLE
DAQIMAGNLGVHYTEIPITELFEQFKQTLHPALQTYPATGPQTTMENLQARIRGTLLMALANQSGRLVLTTSNKSETAVG
YSTLYGDMAGGFSILKDVSKTLVYQLCHYRNRISPIIPERILQRPPSAELRPDQTDQDNLPPYAVLDAIIAAYVENNLSP
AEIIAMNYPEETVRQVLHMIHSCEYKRRQAAPGIRITRRDFGPSWRFPITSGFRQ
>Mature_535_residues
MKIALAQINCMAGDLNGNPLKILHACQQAREAGARLVITPEMALCGSLPEDWLLRAEFVQVCHQALMDLATQIHDVTLVI
GHPHRTNGNLLNAVSVIRDGRLLITYGKNHLLTDPLLDERRYFTPGNQPCTFECDGTLFGLTTFSDYQHPAHLQALRSAD
VQVLLTVGASPYAIDGQADRLRIVRAGITQTGLPTVYTNLVGGQDELVFDGASFAMDHSGKLVCQLAAFQETLGLIELHN
NQLIPGQCSPLPDRIESVYSALRLGLRDFIDKNRISGVLIGLSGGVDSALVLAIAVDALGADRVSNVMMPSPYTADISLE
DAQIMAGNLGVHYTEIPITELFEQFKQTLHPALQTYPATGPQTTMENLQARIRGTLLMALANQSGRLVLTTSNKSETAVG
YSTLYGDMAGGFSILKDVSKTLVYQLCHYRNRISPIIPERILQRPPSAELRPDQTDQDNLPPYAVLDAIIAAYVENNLSP
AEIIAMNYPEETVRQVLHMIHSCEYKRRQAAPGIRITRRDFGPSWRFPITSGFRQ

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 58747; Mature: 58747

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALAQINCMAGDLNGNPLKILHACQQAREAGARLVITPEMALCGSLPEDWLLRAEFVQ
CEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCHHHHHHHHHHH
VCHQALMDLATQIHDVTLVIGHPHRTNGNLLNAVSVIRDGRLLITYGKNHLLTDPLLDER
HHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
RYFTPGNQPCTFECDGTLFGLTTFSDYQHPAHLQALRSADVQVLLTVGASPYAIDGQADR
CCCCCCCCCEEEEECCEEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCEEECCCCCE
LRIVRAGITQTGLPTVYTNLVGGQDELVFDGASFAMDHSGKLVCQLAAFQETLGLIELHN
EEEEECCCCCCCCCHHHHHHCCCCCCEEECCCHHEECCCCCEEEEHHHHHHHHCEEEECC
NQLIPGQCSPLPDRIESVYSALRLGLRDFIDKNRISGVLIGLSGGVDSALVLAIAVDALG
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHC
ADRVSNVMMPSPYTADISLEDAQIMAGNLGVHYTEIPITELFEQFKQTLHPALQTYPATG
HHHHHCCCCCCCCEECEEECCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
PQTTMENLQARIRGTLLMALANQSGRLVLTTSNKSETAVGYSTLYGDMAGGFSILKDVSK
CHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHH
TLVYQLCHYRNRISPIIPERILQRPPSAELRPDQTDQDNLPPYAVLDAIIAAYVENNLSP
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
AEIIAMNYPEETVRQVLHMIHSCEYKRRQAAPGIRITRRDFGPSWRFPITSGFRQ
CEEEEECCCHHHHHHHHHHHHHCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIALAQINCMAGDLNGNPLKILHACQQAREAGARLVITPEMALCGSLPEDWLLRAEFVQ
CEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCHHHHHHHHHHH
VCHQALMDLATQIHDVTLVIGHPHRTNGNLLNAVSVIRDGRLLITYGKNHLLTDPLLDER
HHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
RYFTPGNQPCTFECDGTLFGLTTFSDYQHPAHLQALRSADVQVLLTVGASPYAIDGQADR
CCCCCCCCCEEEEECCEEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCEEECCCCCE
LRIVRAGITQTGLPTVYTNLVGGQDELVFDGASFAMDHSGKLVCQLAAFQETLGLIELHN
EEEEECCCCCCCCCHHHHHHCCCCCCEEECCCHHEECCCCCEEEEHHHHHHHHCEEEECC
NQLIPGQCSPLPDRIESVYSALRLGLRDFIDKNRISGVLIGLSGGVDSALVLAIAVDALG
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHC
ADRVSNVMMPSPYTADISLEDAQIMAGNLGVHYTEIPITELFEQFKQTLHPALQTYPATG
HHHHHCCCCCCCCEECEEECCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
PQTTMENLQARIRGTLLMALANQSGRLVLTTSNKSETAVGYSTLYGDMAGGFSILKDVSK
CHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHH
TLVYQLCHYRNRISPIIPERILQRPPSAELRPDQTDQDNLPPYAVLDAIIAAYVENNLSP
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
AEIIAMNYPEETVRQVLHMIHSCEYKRRQAAPGIRITRRDFGPSWRFPITSGFRQ
CEEEEECCCHHHHHHHHHHHHHCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]