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The map label for this gene is dadA [C]

Identifier: 114331877

GI number: 114331877

Start: 2017310

End: 2018419

Strand: Direct

Name: dadA [C]

Synonym: Neut_1902

Alternate gene names: 114331877

Gene position: 2017310-2018419 (Clockwise)

Preceding gene: 114331871

Following gene: 114331879

Centisome position: 75.81

GC content: 49.1

Gene sequence:

>1110_bases
ATGAGTGATTTTATAGTGATTGGAGCTGGGATTATCGGTCTTGCCACTGCGCTCCGATTACTTGAAGAAGGGGCATCAGT
CACGCTCCTTGAACGTCGCAAAGCTGGCTGCGAGGCATCATGGGCAGGCGGAGGCATTCTGTCCCCTCTGTGCCCCTGGA
ACTATCCGGACACCATAAACCGACTCGTCGCTTTCAGCACATCACTCTATCCAGAGTGGACTGCTGCACTTAGCGCGGCT
ACAGGAATTAATCCGGAATATCACGTATGCGGACTTCTCGCTCTTCCACCCTATGACTTGGATGAAGCTGTTAGCTGGTG
CGCTGCTCATGCGGCACCGTTGACATACCAGATATTTCCACTTTCAACCCATGCTAGAACTCCGGGAATCTACGATTGCA
CCCAGCAGCAAGCTGATCAGGCACTATTGCTTCCTGACGTCGCCCAAGTTCGAAATCCACGTCTGTCACAAGCGTTATGC
AAACGCGTCAAACAGCTCGGTGGAAATATAATTGAACACTGCGAGGTCCAGAAACTGAATGCAACCAGGCAGGAAGTTAG
ATCCATCCGGGTCCCACATAAAAACCTCTCTGCCGATCGATACATCCTGTCAGCCGGTGCATGGAGCAGGAATATTCTTG
GAGAATATGCGTTGAATATTGAAATAAAACCAATCCGAGGACAGATACTGTTATACAAATTCCCGGAAAAACCTTTGCAT
TCTGTCGTATTGCAACAAGACCTTTATCTCATTCCCCGGCGTGATGGACATTTACTCGTCGGCAGCACAACCGAAGATGT
CGGATTCGATAAGCGCATTACACTGGAAGCGAAAGACAAATTATCCAAATGGGCAGGAAAAATATTACCAAAGCTGAGTA
ATATGTTGCCGCTAAGACACTGGTCAGGCTTGCGCCCCGCCACACCTGGCAATATCCCCGTCATCGGGGCACATCCATTC
CTTAAAAACCTTTATGTCAACAGCGGACATTTTCGCTATGGCGTCACGATGGCCCCTGGCAGCGCAGAAATATTAACAAA
CGAGATCATGCAACGAGCTCAACCTTTTGATACCACACCCTATCAGCAAGGATGGAATCTGTCTGGGTAA

Upstream 100 bases:

>100_bases
GCCGAAGTTAAAGAATATTTAACCTCATTAAATCACAAAACAAGCAATTCAGGGGTCACGTTATTCATCTCTCATAAAAC
AACATCTGACAACAGGAATA

Downstream 100 bases:

>100_bases
TTTCAATCAGATATGCTTGTTGAAACCGACTGGCTCAGCCTTTGGTAATGGGAATGTTACGGATTCAAGGACTCCCGCCA
GCTCTTCCACCACCACACCC

Product: glycine oxidase ThiO

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSDFIVIGAGIIGLATALRLLEEGASVTLLERRKAGCEASWAGGGILSPLCPWNYPDTINRLVAFSTSLYPEWTAALSAA
TGINPEYHVCGLLALPPYDLDEAVSWCAAHAAPLTYQIFPLSTHARTPGIYDCTQQQADQALLLPDVAQVRNPRLSQALC
KRVKQLGGNIIEHCEVQKLNATRQEVRSIRVPHKNLSADRYILSAGAWSRNILGEYALNIEIKPIRGQILLYKFPEKPLH
SVVLQQDLYLIPRRDGHLLVGSTTEDVGFDKRITLEAKDKLSKWAGKILPKLSNMLPLRHWSGLRPATPGNIPVIGAHPF
LKNLYVNSGHFRYGVTMAPGSAEILTNEIMQRAQPFDTTPYQQGWNLSG

Sequences:

>Translated_369_residues
MSDFIVIGAGIIGLATALRLLEEGASVTLLERRKAGCEASWAGGGILSPLCPWNYPDTINRLVAFSTSLYPEWTAALSAA
TGINPEYHVCGLLALPPYDLDEAVSWCAAHAAPLTYQIFPLSTHARTPGIYDCTQQQADQALLLPDVAQVRNPRLSQALC
KRVKQLGGNIIEHCEVQKLNATRQEVRSIRVPHKNLSADRYILSAGAWSRNILGEYALNIEIKPIRGQILLYKFPEKPLH
SVVLQQDLYLIPRRDGHLLVGSTTEDVGFDKRITLEAKDKLSKWAGKILPKLSNMLPLRHWSGLRPATPGNIPVIGAHPF
LKNLYVNSGHFRYGVTMAPGSAEILTNEIMQRAQPFDTTPYQQGWNLSG
>Mature_368_residues
SDFIVIGAGIIGLATALRLLEEGASVTLLERRKAGCEASWAGGGILSPLCPWNYPDTINRLVAFSTSLYPEWTAALSAAT
GINPEYHVCGLLALPPYDLDEAVSWCAAHAAPLTYQIFPLSTHARTPGIYDCTQQQADQALLLPDVAQVRNPRLSQALCK
RVKQLGGNIIEHCEVQKLNATRQEVRSIRVPHKNLSADRYILSAGAWSRNILGEYALNIEIKPIRGQILLYKFPEKPLHS
VVLQQDLYLIPRRDGHLLVGSTTEDVGFDKRITLEAKDKLSKWAGKILPKLSNMLPLRHWSGLRPATPGNIPVIGAHPFL
KNLYVNSGHFRYGVTMAPGSAEILTNEIMQRAQPFDTTPYQQGWNLSG

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=400, Percent_Identity=24, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 40702; Mature: 40571

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFIVIGAGIIGLATALRLLEEGASVTLLERRKAGCEASWAGGGILSPLCPWNYPDTIN
CCCEEEECCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
RLVAFSTSLYPEWTAALSAATGINPEYHVCGLLALPPYDLDEAVSWCAAHAAPLTYQIFP
HHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
LSTHARTPGIYDCTQQQADQALLLPDVAQVRNPRLSQALCKRVKQLGGNIIEHCEVQKLN
CCCCCCCCCCCHHHHHHCCCEEECCCHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
ATRQEVRSIRVPHKNLSADRYILSAGAWSRNILGEYALNIEIKPIRGQILLYKFPEKPLH
HHHHHHHHHCCCCCCCCCCEEEEECCCCCHHCCCCEEEEEEEEECCCEEEEEECCCCHHH
SVVLQQDLYLIPRRDGHLLVGSTTEDVGFDKRITLEAKDKLSKWAGKILPKLSNMLPLRH
HHHHHCCEEEEECCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHH
WSGLRPATPGNIPVIGAHPFLKNLYVNSGHFRYGVTMAPGSAEILTNEIMQRAQPFDTTP
CCCCCCCCCCCCEEEECCHHHHHHEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCC
YQQGWNLSG
CCCCCCCCC
>Mature Secondary Structure 
SDFIVIGAGIIGLATALRLLEEGASVTLLERRKAGCEASWAGGGILSPLCPWNYPDTIN
CCEEEECCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
RLVAFSTSLYPEWTAALSAATGINPEYHVCGLLALPPYDLDEAVSWCAAHAAPLTYQIFP
HHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
LSTHARTPGIYDCTQQQADQALLLPDVAQVRNPRLSQALCKRVKQLGGNIIEHCEVQKLN
CCCCCCCCCCCHHHHHHCCCEEECCCHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
ATRQEVRSIRVPHKNLSADRYILSAGAWSRNILGEYALNIEIKPIRGQILLYKFPEKPLH
HHHHHHHHHCCCCCCCCCCEEEEECCCCCHHCCCCEEEEEEEEECCCEEEEEECCCCHHH
SVVLQQDLYLIPRRDGHLLVGSTTEDVGFDKRITLEAKDKLSKWAGKILPKLSNMLPLRH
HHHHHCCEEEEECCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHH
WSGLRPATPGNIPVIGAHPFLKNLYVNSGHFRYGVTMAPGSAEILTNEIMQRAQPFDTTP
CCCCCCCCCCCCEEEECCHHHHHHEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCC
YQQGWNLSG
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]