Click here to switch to the map view.

The map label for this gene is amiC [H]

Identifier: 114331871

GI number: 114331871

Start: 2011506

End: 2012813

Strand: Direct

Name: amiC [H]

Synonym: Neut_1896

Alternate gene names: 114331871

Gene position: 2011506-2012813 (Clockwise)

Preceding gene: 114331870

Following gene: 114331877

Centisome position: 75.59

GC content: 46.87

Gene sequence:

>1308_bases
ATGAATCAACCCGGAACCAGGCAGTGCTCTCCATTTCTACTTTTCGTTATAGCTATCCTCCTGCAACTGGCTTTGTATAG
CAACGATACATCAGCTGGCACTCAAATCACGGCAGCACGCTACTGGGCGGGGCCCGAGTATACCCGGTTGACACTGGAAT
CGAGTAAGCCGGTTAAATACTCGGTCAGCACACTCCAATCACCCAAAAGAATCGTGATGGACATGGAGAATATTTCATTG
TCTGATGTACTGAAGAGCTTGCCAGCAAAGACCGAATCACGGGATCCCCTGGTTGGTGCATTAAGAGTCGGCAATTTCAA
TTCGCGGACTGTCAGGCTGGTCGTGGAATTAAAAACAGATGCTGTACCCAAAGCATTCGCGCTTGATCCTGTAGACCAAT
TTGGGCATCGGCTGGTGCTGGATCTTTACCCATCGAAAAAACTGATAACAGAACCGTATGAACAGGACCCACTCATAGCA
TTAATACAAAAAAACAAGAAACCTGCGCCAGTCGCTCATTCACACAATGTAACCCCCGCTGCGACTTCGAAGACTGTCCT
GACAGCAACCAGCCGCAAACCTGAAAAGAACCGGATTATTACTATCGCAATTGACGCCGGACATGGCGGCAAGGATCCGG
GAGCAATTGGTCCGCAAGGCTCCATGGAAAAAAATATAACCCTTTCCATCGCCAGAAAACTCAAGGCCAGAATTGACAAG
GAACCGGGTATGCGTTCCGTACTAATTCGTGACGGAGATTACTTCATATCGCTGGCTGAACGGCGCATCAAGGCAAGACA
AGCCAATGCCGATTTATTTGTATCTATTCATGCTGATGCTGCTCCACGGAGAGAAGCACATGGTGCATCAATTTACGCAT
TATCAGAAAATGGTGCCACTTCCACCACAGCGAGCTGGCTGGCCAAAAAGGAAAATGCGGTTGATCTGATCGGCGGAGTC
AAACTGGATAACAAAGATCGATACCTAAAGCAAACACTGATTGATCTGTCCATGAATGCCACGATTAATGACAGTATCCG
GCTGGCCAATCACGTATTGAATGAAATCGGGACAATCAGCCATCTGCACAAAAAAAATGTGGAACAGGCTGGTTTTGCAG
TACTCAAATCACCTGATATTCCATCTGTTCTGGTTGAAACCGCATTCATCAGCAACCAGACTGAAGAAGCCAAACTTAAT
TCAGAGATTCACCAGAATAAATTGGTTGATGCGATATCAGTCGGACTTAAACGCTATTTCAACAGCGGATCCTGGCAGAC
ACGGATGGATGTTGCTGATACCAGATAA

Upstream 100 bases:

>100_bases
TATGGATCAGCTATTCTGGAACAGGAAGAATCGCCGAACTCAAGGCGGAAACGGAGGCAGGAGAACAATGCTTATCACGC
TGGCAAAAACAGGTATCCGA

Downstream 100 bases:

>100_bases
GAGATATCAGCCTCTACCGGTTCTCAGACTACACTGTTTTATAGAGATGTCTGCTTATGGGTAAAACAGCTCCAGTTTGT
AGCCCATAGCATTGAGCTGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNQPGTRQCSPFLLFVIAILLQLALYSNDTSAGTQITAARYWAGPEYTRLTLESSKPVKYSVSTLQSPKRIVMDMENISL
SDVLKSLPAKTESRDPLVGALRVGNFNSRTVRLVVELKTDAVPKAFALDPVDQFGHRLVLDLYPSKKLITEPYEQDPLIA
LIQKNKKPAPVAHSHNVTPAATSKTVLTATSRKPEKNRIITIAIDAGHGGKDPGAIGPQGSMEKNITLSIARKLKARIDK
EPGMRSVLIRDGDYFISLAERRIKARQANADLFVSIHADAAPRREAHGASIYALSENGATSTTASWLAKKENAVDLIGGV
KLDNKDRYLKQTLIDLSMNATINDSIRLANHVLNEIGTISHLHKKNVEQAGFAVLKSPDIPSVLVETAFISNQTEEAKLN
SEIHQNKLVDAISVGLKRYFNSGSWQTRMDVADTR

Sequences:

>Translated_435_residues
MNQPGTRQCSPFLLFVIAILLQLALYSNDTSAGTQITAARYWAGPEYTRLTLESSKPVKYSVSTLQSPKRIVMDMENISL
SDVLKSLPAKTESRDPLVGALRVGNFNSRTVRLVVELKTDAVPKAFALDPVDQFGHRLVLDLYPSKKLITEPYEQDPLIA
LIQKNKKPAPVAHSHNVTPAATSKTVLTATSRKPEKNRIITIAIDAGHGGKDPGAIGPQGSMEKNITLSIARKLKARIDK
EPGMRSVLIRDGDYFISLAERRIKARQANADLFVSIHADAAPRREAHGASIYALSENGATSTTASWLAKKENAVDLIGGV
KLDNKDRYLKQTLIDLSMNATINDSIRLANHVLNEIGTISHLHKKNVEQAGFAVLKSPDIPSVLVETAFISNQTEEAKLN
SEIHQNKLVDAISVGLKRYFNSGSWQTRMDVADTR
>Mature_435_residues
MNQPGTRQCSPFLLFVIAILLQLALYSNDTSAGTQITAARYWAGPEYTRLTLESSKPVKYSVSTLQSPKRIVMDMENISL
SDVLKSLPAKTESRDPLVGALRVGNFNSRTVRLVVELKTDAVPKAFALDPVDQFGHRLVLDLYPSKKLITEPYEQDPLIA
LIQKNKKPAPVAHSHNVTPAATSKTVLTATSRKPEKNRIITIAIDAGHGGKDPGAIGPQGSMEKNITLSIARKLKARIDK
EPGMRSVLIRDGDYFISLAERRIKARQANADLFVSIHADAAPRREAHGASIYALSENGATSTTASWLAKKENAVDLIGGV
KLDNKDRYLKQTLIDLSMNATINDSIRLANHVLNEIGTISHLHKKNVEQAGFAVLKSPDIPSVLVETAFISNQTEEAKLN
SEIHQNKLVDAISVGLKRYFNSGSWQTRMDVADTR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=398, Percent_Identity=44.9748743718593, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1790611, Length=393, Percent_Identity=38.4223918575064, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1788776, Length=220, Percent_Identity=45, Blast_Score=187, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 47719; Mature: 47719

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPGTRQCSPFLLFVIAILLQLALYSNDTSAGTQITAARYWAGPEYTRLTLESSKPVKY
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCEE
SVSTLQSPKRIVMDMENISLSDVLKSLPAKTESRDPLVGALRVGNFNSRTVRLVVELKTD
EHHHHCCCHHEEEECCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCC
AVPKAFALDPVDQFGHRLVLDLYPSKKLITEPYEQDPLIALIQKNKKPAPVAHSHNVTPA
CCCCEEECCCHHHCCCEEEEEECCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
ATSKTVLTATSRKPEKNRIITIAIDAGHGGKDPGAIGPQGSMEKNITLSIARKLKARIDK
CCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
EPGMRSVLIRDGDYFISLAERRIKARQANADLFVSIHADAAPRREAHGASIYALSENGAT
CCCCEEEEEECCCCEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCC
STTASWLAKKENAVDLIGGVKLDNKDRYLKQTLIDLSMNATINDSIRLANHVLNEIGTIS
CHHHHHHHHCCCCEEEEECEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
HLHKKNVEQAGFAVLKSPDIPSVLVETAFISNQTEEAKLNSEIHQNKLVDAISVGLKRYF
HHHHCCHHHHCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NSGSWQTRMDVADTR
CCCCCEEECCCCCCC
>Mature Secondary Structure
MNQPGTRQCSPFLLFVIAILLQLALYSNDTSAGTQITAARYWAGPEYTRLTLESSKPVKY
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCEE
SVSTLQSPKRIVMDMENISLSDVLKSLPAKTESRDPLVGALRVGNFNSRTVRLVVELKTD
EHHHHCCCHHEEEECCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCC
AVPKAFALDPVDQFGHRLVLDLYPSKKLITEPYEQDPLIALIQKNKKPAPVAHSHNVTPA
CCCCEEECCCHHHCCCEEEEEECCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
ATSKTVLTATSRKPEKNRIITIAIDAGHGGKDPGAIGPQGSMEKNITLSIARKLKARIDK
CCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
EPGMRSVLIRDGDYFISLAERRIKARQANADLFVSIHADAAPRREAHGASIYALSENGAT
CCCCEEEEEECCCCEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCC
STTASWLAKKENAVDLIGGVKLDNKDRYLKQTLIDLSMNATINDSIRLANHVLNEIGTIS
CHHHHHHHHCCCCEEEEECEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
HLHKKNVEQAGFAVLKSPDIPSVLVETAFISNQTEEAKLNSEIHQNKLVDAISVGLKRYF
HHHHCCHHHHCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NSGSWQTRMDVADTR
CCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]