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The map label for this gene is metF [H]

Identifier: 114331866

GI number: 114331866

Start: 2006125

End: 2006979

Strand: Reverse

Name: metF [H]

Synonym: Neut_1891

Alternate gene names: 114331866

Gene position: 2006979-2006125 (Counterclockwise)

Preceding gene: 114331867

Following gene: 114331864

Centisome position: 75.42

GC content: 53.1

Gene sequence:

>855_bases
ATGTTATCCCAGAAAAATTTTACGCCCACATTCAGTTTTGAGTTTTTCCCGCCGCAGACACCGGAAGGCATGGAAAAACT
GCGGGCAACGCGTATACAACTTGCCCGCTTCAACCCGAAGTTCTTTTCGGTAACATTCGGTGCAGGTGGCTCCACTCGGG
AGCGGACGCTGGAAACCGTGCTGGAAATCCAGGCAGAAGGTTCCCCGGTAGCGCCGCATCTATCCTGTATCGGTTCCACC
CGCGACAATATCCGCTCCATTCTTGAAAAATATCACAGCCACAATATCCGCCACATTATCGCGTTGCGTGGTGATCTGCC
TTCGGGCATGGCGCAGGCGGGTGAATTCCGCTATGCCAATGAGCTGGTGGCATTTATCCGCAAGGAGTTCGGCGATACTT
TCTGGATCGAAGTGGCAGCTTATCCGGAATATCATCCACAGGCACGTTCCGCACTGGAGGATTTCACCAACTTCAGACGA
AAAGTTGAGGCAGGTGCCAATGCAGCGATTACCCAGTTTTTCTATAACGTCGATGCCTATCTGCATTTCGTAGAGATGTG
CGAAGCTGCGAATATCAGTGTCCCAATCGTTCCCGGCATCATGCCGATCAGCAAATTTTCCCAACTGGCAAGATTTTCCG
ATGGCTGTGGAGCGGAGATACCCCGCTGGATTCGCAAAAAACTGGAGAGCTTTGGTGATGACATCCCTTCCATCCAGGCA
TTCGGGCTGGATGTGGTCACCGCACTTTGTGCGCGCCTGCTGGAAGCCGGTGCACCCGGCCTGCATTTCTACACGCTTAA
CTCGGCCGTACTACCCACAAAAATCTGGCAGCGGTTGGGGCTAACGGGGCATTGA

Upstream 100 bases:

>100_bases
TTTCGCTGCTGCAAGGATCTGATGGAAAATTTGGGTTTTATTTCATAAATTCTCACTACTGCTTCCTTTGAAGGGAAGTG
GCCAATGGAAGTAAGTCCAG

Downstream 100 bases:

>100_bases
AAAAAATGTAACGGCTTTCTGCGGATTATTATTTGGTAGCCAAAGAGAAAGGTTAGATCATTTTGAATTTACTTGTTTAT
ATATCCACATGAAGCAAGGT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MLSQKNFTPTFSFEFFPPQTPEGMEKLRATRIQLARFNPKFFSVTFGAGGSTRERTLETVLEIQAEGSPVAPHLSCIGST
RDNIRSILEKYHSHNIRHIIALRGDLPSGMAQAGEFRYANELVAFIRKEFGDTFWIEVAAYPEYHPQARSALEDFTNFRR
KVEAGANAAITQFFYNVDAYLHFVEMCEAANISVPIVPGIMPISKFSQLARFSDGCGAEIPRWIRKKLESFGDDIPSIQA
FGLDVVTALCARLLEAGAPGLHFYTLNSAVLPTKIWQRLGLTGH

Sequences:

>Translated_284_residues
MLSQKNFTPTFSFEFFPPQTPEGMEKLRATRIQLARFNPKFFSVTFGAGGSTRERTLETVLEIQAEGSPVAPHLSCIGST
RDNIRSILEKYHSHNIRHIIALRGDLPSGMAQAGEFRYANELVAFIRKEFGDTFWIEVAAYPEYHPQARSALEDFTNFRR
KVEAGANAAITQFFYNVDAYLHFVEMCEAANISVPIVPGIMPISKFSQLARFSDGCGAEIPRWIRKKLESFGDDIPSIQA
FGLDVVTALCARLLEAGAPGLHFYTLNSAVLPTKIWQRLGLTGH
>Mature_284_residues
MLSQKNFTPTFSFEFFPPQTPEGMEKLRATRIQLARFNPKFFSVTFGAGGSTRERTLETVLEIQAEGSPVAPHLSCIGST
RDNIRSILEKYHSHNIRHIIALRGDLPSGMAQAGEFRYANELVAFIRKEFGDTFWIEVAAYPEYHPQARSALEDFTNFRR
KVEAGANAAITQFFYNVDAYLHFVEMCEAANISVPIVPGIMPISKFSQLARFSDGCGAEIPRWIRKKLESFGDDIPSIQA
FGLDVVTALCARLLEAGAPGLHFYTLNSAVLPTKIWQRLGLTGH

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=280, Percent_Identity=37.1428571428571, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI1790377, Length=271, Percent_Identity=43.5424354243542, Blast_Score=226, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI25147436, Length=281, Percent_Identity=32.7402135231317, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25147441, Length=281, Percent_Identity=32.7402135231317, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6325234, Length=279, Percent_Identity=36.2007168458781, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321313, Length=290, Percent_Identity=33.7931034482759, Blast_Score=159, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24662658, Length=215, Percent_Identity=28.3720930232558, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31753; Mature: 31753

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSQKNFTPTFSFEFFPPQTPEGMEKLRATRIQLARFNPKFFSVTFGAGGSTRERTLETV
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
LEIQAEGSPVAPHLSCIGSTRDNIRSILEKYHSHNIRHIIALRGDLPSGMAQAGEFRYAN
HHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCHHHHH
ELVAFIRKEFGDTFWIEVAAYPEYHPQARSALEDFTNFRRKVEAGANAAITQFFYNVDAY
HHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LHFVEMCEAANISVPIVPGIMPISKFSQLARFSDGCGAEIPRWIRKKLESFGDDIPSIQA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHH
FGLDVVTALCARLLEAGAPGLHFYTLNSAVLPTKIWQRLGLTGH
HHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLSQKNFTPTFSFEFFPPQTPEGMEKLRATRIQLARFNPKFFSVTFGAGGSTRERTLETV
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
LEIQAEGSPVAPHLSCIGSTRDNIRSILEKYHSHNIRHIIALRGDLPSGMAQAGEFRYAN
HHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCHHHHH
ELVAFIRKEFGDTFWIEVAAYPEYHPQARSALEDFTNFRRKVEAGANAAITQFFYNVDAY
HHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LHFVEMCEAANISVPIVPGIMPISKFSQLARFSDGCGAEIPRWIRKKLESFGDDIPSIQA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHH
FGLDVVTALCARLLEAGAPGLHFYTLNSAVLPTKIWQRLGLTGH
HHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]