Click here to switch to the map view.

The map label for this gene is pdxJ

Identifier: 114331753

GI number: 114331753

Start: 1881511

End: 1882236

Strand: Reverse

Name: pdxJ

Synonym: Neut_1776

Alternate gene names: 114331753

Gene position: 1882236-1881511 (Counterclockwise)

Preceding gene: 114331754

Following gene: 114331752

Centisome position: 70.73

GC content: 50.55

Gene sequence:

>726_bases
ATGATAGCTCTGGGTGTCAATATTGATCATGTCGCAACGCTACGGCAGGCACGAGGTACGGATTATCCCAGTCCGATCAA
GGCAGCTCTGGTTGCCGAGGAATCTGGAGCGGATGCGATTACGCTACATTTGCGTGAAGATCGGCGTCATATCCAGGAAG
AGGATGTGATAATTCTGCGAGAACGACTTAAAACCCGAATGAACCTGGAAAGCGCTGTAACAGAAGAGATGATCTCTTTT
GCCTGCAGAATCAAACCACATGATATCTGCTTGGTGCCGGAGCGTCGTGAAGAGTTGACCACTGAAGGGGGACTGGATGT
GATCCGGCACTTCGATCAGGTAAGTGCCGCGTGCAAGCGCCTCACAGAAGCTGGAATTCGTGTTTCTCTGTTTGTTGATG
CACGAGCTGATCAGATTGATGCAGCAATCAGGGTGGGCGCGCCAGTGATTGAGCTACATACCGGCCATTATGCAGATGCT
GCAACATCTGAGGCACAACAGGCTGAGCTGGAAACAATCCGCAGTATGGCGGCCTATGCATTTAGCCGGGGATTACAGGT
GAATGCTGGACACAGCTTGCATTACCAGAACACCACGCCAATTGCATTTATCCCGGAGATAACCGAATTGAATATCGGCC
ATGCTATTGTCGCCCGGGCACTGTTTATTGGTTTTGCTCAAGCTGTACGGGAAATGAAAACGTTAATGCAAGAGGCGCGT
GCATGA

Upstream 100 bases:

>100_bases
GCAGACTATCTGGCACATACCCCCAACCGGGTTTGTAGATCAGCTGATTTGTAAAAGATTAAAAAAAGCAATTTAAAACG
ACTGGGAGAACTGAATAATC

Downstream 100 bases:

>100_bases
TATACGGGATTGGTACCGATCTGGTTGATCCTGCCCGTATTGCGGGGTCGCTGGAGCGTTACGGAGAAAGATTTGCCAGA
CGCGTGCTGGCAGATTCTGA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MIALGVNIDHVATLRQARGTDYPSPIKAALVAEESGADAITLHLREDRRHIQEEDVIILRERLKTRMNLESAVTEEMISF
ACRIKPHDICLVPERREELTTEGGLDVIRHFDQVSAACKRLTEAGIRVSLFVDARADQIDAAIRVGAPVIELHTGHYADA
ATSEAQQAELETIRSMAAYAFSRGLQVNAGHSLHYQNTTPIAFIPEITELNIGHAIVARALFIGFAQAVREMKTLMQEAR
A

Sequences:

>Translated_241_residues
MIALGVNIDHVATLRQARGTDYPSPIKAALVAEESGADAITLHLREDRRHIQEEDVIILRERLKTRMNLESAVTEEMISF
ACRIKPHDICLVPERREELTTEGGLDVIRHFDQVSAACKRLTEAGIRVSLFVDARADQIDAAIRVGAPVIELHTGHYADA
ATSEAQQAELETIRSMAAYAFSRGLQVNAGHSLHYQNTTPIAFIPEITELNIGHAIVARALFIGFAQAVREMKTLMQEAR
A
>Mature_241_residues
MIALGVNIDHVATLRQARGTDYPSPIKAALVAEESGADAITLHLREDRRHIQEEDVIILRERLKTRMNLESAVTEEMISF
ACRIKPHDICLVPERREELTTEGGLDVIRHFDQVSAACKRLTEAGIRVSLFVDARADQIDAAIRVGAPVIELHTGHYADA
ATSEAQQAELETIRSMAAYAFSRGLQVNAGHSLHYQNTTPIAFIPEITELNIGHAIVARALFIGFAQAVREMKTLMQEAR
A

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=238, Percent_Identity=62.6050420168067, Blast_Score=297, Evalue=3e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_NITEC (Q0AF72)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747975.1
- ProteinModelPortal:   Q0AF72
- SMR:   Q0AF72
- STRING:   Q0AF72
- GeneID:   4273125
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1776
- NMPDR:   fig|335283.3.peg.2009
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q0AF72
- ProtClustDB:   PRK05265
- BioCyc:   NEUT335283:NEUT_1776-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26643; Mature: 26643

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: ACT_SITE 43-43 ACT_SITE 70-70 ACT_SITE 191-191 BINDING 7-7 BINDING 18-18 BINDING 45-45 BINDING 50-50 BINDING 100-100 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALGVNIDHVATLRQARGTDYPSPIKAALVAEESGADAITLHLREDRRHIQEEDVIILR
CEEECCCHHHHHHHHHHCCCCCCCCHHHHEEECCCCCCEEEEEEEHHHHCCCCCCHHHHH
ERLKTRMNLESAVTEEMISFACRIKPHDICLVPERREELTTEGGLDVIRHFDQVSAACKR
HHHHHHHCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHH
LTEAGIRVSLFVDARADQIDAAIRVGAPVIELHTGHYADAATSEAQQAELETIRSMAAYA
HHHCCCEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FSRGLQVNAGHSLHYQNTTPIAFIPEITELNIGHAIVARALFIGFAQAVREMKTLMQEAR
HHCCCEECCCCEEEECCCCCEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure
MIALGVNIDHVATLRQARGTDYPSPIKAALVAEESGADAITLHLREDRRHIQEEDVIILR
CEEECCCHHHHHHHHHHCCCCCCCCHHHHEEECCCCCCEEEEEEEHHHHCCCCCCHHHHH
ERLKTRMNLESAVTEEMISFACRIKPHDICLVPERREELTTEGGLDVIRHFDQVSAACKR
HHHHHHHCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHH
LTEAGIRVSLFVDARADQIDAAIRVGAPVIELHTGHYADAATSEAQQAELETIRSMAAYA
HHHCCCEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FSRGLQVNAGHSLHYQNTTPIAFIPEITELNIGHAIVARALFIGFAQAVREMKTLMQEAR
HHCCCEECCCCEEEECCCCCEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA