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The map label for this gene is odhL [H]

Identifier: 114331750

GI number: 114331750

Start: 1878554

End: 1880014

Strand: Reverse

Name: odhL [H]

Synonym: Neut_1773

Alternate gene names: 114331750

Gene position: 1880014-1878554 (Counterclockwise)

Preceding gene: 114331751

Following gene: 114331749

Centisome position: 70.65

GC content: 48.94

Gene sequence:

>1461_bases
ATGAATAATATATTCGATGTAGCAGTTATCGGCGCAGGGCCTGGCGGCTATGTAGCAGCTATCCGCTGTGCGCAGCTCGG
GCTAAACACAGTATGTATTGATGACTGGAAGAATGAGCAGGGCAGACCAAGCTTGGGGGGTACCTGTCTTAACGTGGGCT
GTATTCCTTCCAAAGCACTGCTTGAGTCTTCCGAGAATTTTGCGCGAGCGGGACATAAATTTGCGGAACACGGCATTAAG
CTGGATGGATTATCGATAGATGTTCCAGCCATGATTGCCCGCAAGGACAAGATCGTCAAAGCTTTTACTGGCGGGATTGG
CATGCTGTTCAAGAAAAATAAAGTAACTGTCTTGCATGGTCGTGGCGTACTGCAAAAGCGCGATAACGACGATGATAGCT
GGGAAATAAGAGTAAAAACTGACGAGAAGGAGCAGTCAGTACGCACCAGGCATGTCATCATTGCGACAGGCTCTGTTCCT
CGTTCTCTGACAATAGCGCCAGTTGACGGGGTTAATGTACTTGATAATGCAGGTGCTCTGGCTTTGCAACAAACTCCTGG
AAAACTTGCCATTATCGGTGCGGGAGTGATTGGCTTGGAACTTGGAAGTGTCTGGCGCAGGCTGGGAGCAGAAGTAACAA
TACTGGAAGCCCAAGCTGATTTTCTGTCAGCAGCAGATGAGCAAGTCGCCAAGGAAGCATACAAGGCATTGACGCGTGAA
ACCGGACTTGTCATTCATACCGGGGTTGAGATTAAATCAACTCAAGCAGGCAAGGATAATGTAAAAATCGAGTATACCGA
TCGTGACAAGAACGTACAGAGCCTGGAGGTGGACAAACTCATCGTGGCTGTGGGGCGTGTTCCCAATACCGCTAGTTTGG
GAGCTAAAGAAACAGGATTACAGCTGGATGAGCGCGGATATATTGGTGTGGATAAATTTTGCCAAACCAGTTTGCAAAAT
GTATATGCAATTGGAGATGTTGTAAGAGGGCCAATGCTGGCGCATAAGGCTTCTGAAGAAGGAGTTGCTGTTGCGGAGCG
GATAGCGTCGAACCAGCAAGGAGCATCTGATTCAACCGCGCATATTGATCTCGGAATGATGCCATGGGTGATTTATACCG
CACCGGAGATTGCTTGGGTGGGAAAAACCGAACAGGCGCTGAAGGCAGAAGGTGTTATCTACAAAGTAGGGCAGTTTCCG
TTCATGGCTAACGGACGTGCTCGCGCACTGGGGGAAACAACAGGGTTTGTTAAAGTTCTGGCGGATGCAGAAAGCGATCG
TATCCTCGGCGTACATATGGTGGGTCCTTATGTATCTGAAATAATTGCCGAAGCTGTGGTTGCCATGGGGTTTTCTGCCA
GCAGCGAGGATCTGGCACGTATCGTACATGCTCATCCATCACTTTCCGAATCACTGCATGAAGCTGCATTAGGCGTGGCT
AAACGGACCATTCATATCTGA

Upstream 100 bases:

>100_bases
CTGGCTAATATCAGTGTATCCGTGTGAGAATTGCATTTTTAGCGTTCCGGGGTAATTCAGACCGGATTTCTCAACGAGCA
ATTTTTGTCACTTAATTGAT

Downstream 100 bases:

>100_bases
TTTGCGAAATATTGATCTGGATTTTCTGGGAGCAAATAGCATGAAACTGCTGTTTTTTGGTTTGCTGCTGATCTCCATGT
CTGTATTTGCAGCAGCGGAC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MNNIFDVAVIGAGPGGYVAAIRCAQLGLNTVCIDDWKNEQGRPSLGGTCLNVGCIPSKALLESSENFARAGHKFAEHGIK
LDGLSIDVPAMIARKDKIVKAFTGGIGMLFKKNKVTVLHGRGVLQKRDNDDDSWEIRVKTDEKEQSVRTRHVIIATGSVP
RSLTIAPVDGVNVLDNAGALALQQTPGKLAIIGAGVIGLELGSVWRRLGAEVTILEAQADFLSAADEQVAKEAYKALTRE
TGLVIHTGVEIKSTQAGKDNVKIEYTDRDKNVQSLEVDKLIVAVGRVPNTASLGAKETGLQLDERGYIGVDKFCQTSLQN
VYAIGDVVRGPMLAHKASEEGVAVAERIASNQQGASDSTAHIDLGMMPWVIYTAPEIAWVGKTEQALKAEGVIYKVGQFP
FMANGRARALGETTGFVKVLADAESDRILGVHMVGPYVSEIIAEAVVAMGFSASSEDLARIVHAHPSLSESLHEAALGVA
KRTIHI

Sequences:

>Translated_486_residues
MNNIFDVAVIGAGPGGYVAAIRCAQLGLNTVCIDDWKNEQGRPSLGGTCLNVGCIPSKALLESSENFARAGHKFAEHGIK
LDGLSIDVPAMIARKDKIVKAFTGGIGMLFKKNKVTVLHGRGVLQKRDNDDDSWEIRVKTDEKEQSVRTRHVIIATGSVP
RSLTIAPVDGVNVLDNAGALALQQTPGKLAIIGAGVIGLELGSVWRRLGAEVTILEAQADFLSAADEQVAKEAYKALTRE
TGLVIHTGVEIKSTQAGKDNVKIEYTDRDKNVQSLEVDKLIVAVGRVPNTASLGAKETGLQLDERGYIGVDKFCQTSLQN
VYAIGDVVRGPMLAHKASEEGVAVAERIASNQQGASDSTAHIDLGMMPWVIYTAPEIAWVGKTEQALKAEGVIYKVGQFP
FMANGRARALGETTGFVKVLADAESDRILGVHMVGPYVSEIIAEAVVAMGFSASSEDLARIVHAHPSLSESLHEAALGVA
KRTIHI
>Mature_486_residues
MNNIFDVAVIGAGPGGYVAAIRCAQLGLNTVCIDDWKNEQGRPSLGGTCLNVGCIPSKALLESSENFARAGHKFAEHGIK
LDGLSIDVPAMIARKDKIVKAFTGGIGMLFKKNKVTVLHGRGVLQKRDNDDDSWEIRVKTDEKEQSVRTRHVIIATGSVP
RSLTIAPVDGVNVLDNAGALALQQTPGKLAIIGAGVIGLELGSVWRRLGAEVTILEAQADFLSAADEQVAKEAYKALTRE
TGLVIHTGVEIKSTQAGKDNVKIEYTDRDKNVQSLEVDKLIVAVGRVPNTASLGAKETGLQLDERGYIGVDKFCQTSLQN
VYAIGDVVRGPMLAHKASEEGVAVAERIASNQQGASDSTAHIDLGMMPWVIYTAPEIAWVGKTEQALKAEGVIYKVGQFP
FMANGRARALGETTGFVKVLADAESDRILGVHMVGPYVSEIIAEAVVAMGFSASSEDLARIVHAHPSLSESLHEAALGVA
KRTIHI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=480, Percent_Identity=45.2083333333333, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI50301238, Length=475, Percent_Identity=25.8947368421053, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519430, Length=459, Percent_Identity=24.1830065359477, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI33519428, Length=459, Percent_Identity=24.1830065359477, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI33519426, Length=459, Percent_Identity=24.1830065359477, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI148277065, Length=457, Percent_Identity=24.0700218818381, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI148277071, Length=457, Percent_Identity=24.0700218818381, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI22035672, Length=482, Percent_Identity=25.7261410788382, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI291045266, Length=455, Percent_Identity=23.2967032967033, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786307, Length=470, Percent_Identity=35.9574468085106, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI87082354, Length=483, Percent_Identity=29.399585921325, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081717, Length=487, Percent_Identity=28.1314168377823, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=27.5462962962963, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32565766, Length=478, Percent_Identity=45.81589958159, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17557007, Length=497, Percent_Identity=28.3702213279678, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=441, Percent_Identity=24.9433106575964, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71983429, Length=441, Percent_Identity=24.9433106575964, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982272, Length=495, Percent_Identity=25.2525252525253, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=496, Percent_Identity=43.1451612903226, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6325240, Length=486, Percent_Identity=31.2757201646091, Blast_Score=209, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6325166, Length=479, Percent_Identity=27.7661795407098, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21358499, Length=479, Percent_Identity=45.5114822546973, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24640549, Length=499, Percent_Identity=26.0521042084168, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640551, Length=499, Percent_Identity=26.0521042084168, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640553, Length=499, Percent_Identity=26.0521042084168, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17737741, Length=496, Percent_Identity=23.991935483871, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51756; Mature: 51756

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIFDVAVIGAGPGGYVAAIRCAQLGLNTVCIDDWKNEQGRPSLGGTCLNVGCIPSKAL
CCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCHHH
LESSENFARAGHKFAEHGIKLDGLSIDVPAMIARKDKIVKAFTGGIGMLFKKNKVTVLHG
HHCCHHHHHHHHHHHHCCEEECCEEECCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEC
RGVLQKRDNDDDSWEIRVKTDEKEQSVRTRHVIIATGSVPRSLTIAPVDGVNVLDNAGAL
CCCCCCCCCCCCCEEEEEECCCHHHCCCEEEEEEEECCCCCEEEEEECCCCCEECCCCCE
ALQQTPGKLAIIGAGVIGLELGSVWRRLGAEVTILEAQADFLSAADEQVAKEAYKALTRE
EEECCCCCEEEEECCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHC
TGLVIHTGVEIKSTQAGKDNVKIEYTDRDKNVQSLEVDKLIVAVGRVPNTASLGAKETGL
CCEEEEECCEEECCCCCCCCEEEEEECCCCCCCCEEHHEEEEEECCCCCCCCCCCCCCCC
QLDERGYIGVDKFCQTSLQNVYAIGDVVRGPMLAHKASEEGVAVAERIASNQQGASDSTA
EECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCE
HIDLGMMPWVIYTAPEIAWVGKTEQALKAEGVIYKVGQFPFMANGRARALGETTGFVKVL
EEECCCCCEEEEECCCEEEECCCHHHHHHCCEEEEECCCCEEECCCCCCCCCCCCEEEEE
ADAESDRILGVHMVGPYVSEIIAEAVVAMGFSASSEDLARIVHAHPSLSESLHEAALGVA
ECCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
KRTIHI
HHEECC
>Mature Secondary Structure
MNNIFDVAVIGAGPGGYVAAIRCAQLGLNTVCIDDWKNEQGRPSLGGTCLNVGCIPSKAL
CCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCHHH
LESSENFARAGHKFAEHGIKLDGLSIDVPAMIARKDKIVKAFTGGIGMLFKKNKVTVLHG
HHCCHHHHHHHHHHHHCCEEECCEEECCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEC
RGVLQKRDNDDDSWEIRVKTDEKEQSVRTRHVIIATGSVPRSLTIAPVDGVNVLDNAGAL
CCCCCCCCCCCCCEEEEEECCCHHHCCCEEEEEEEECCCCCEEEEEECCCCCEECCCCCE
ALQQTPGKLAIIGAGVIGLELGSVWRRLGAEVTILEAQADFLSAADEQVAKEAYKALTRE
EEECCCCCEEEEECCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHC
TGLVIHTGVEIKSTQAGKDNVKIEYTDRDKNVQSLEVDKLIVAVGRVPNTASLGAKETGL
CCEEEEECCEEECCCCCCCCEEEEEECCCCCCCCEEHHEEEEEECCCCCCCCCCCCCCCC
QLDERGYIGVDKFCQTSLQNVYAIGDVVRGPMLAHKASEEGVAVAERIASNQQGASDSTA
EECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCE
HIDLGMMPWVIYTAPEIAWVGKTEQALKAEGVIYKVGQFPFMANGRARALGETTGFVKVL
EEECCCCCEEEEECCCEEEECCCHHHHHHCCEEEEECCCCEEECCCCCCCCCCCCEEEEE
ADAESDRILGVHMVGPYVSEIIAEAVVAMGFSASSEDLARIVHAHPSLSESLHEAALGVA
ECCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
KRTIHI
HHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]