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The map label for this gene is leuA

Identifier: 114331240

GI number: 114331240

Start: 1299964

End: 1301496

Strand: Direct

Name: leuA

Synonym: Neut_1244

Alternate gene names: 114331240

Gene position: 1299964-1301496 (Clockwise)

Preceding gene: 114331231

Following gene: 114331241

Centisome position: 48.85

GC content: 47.81

Gene sequence:

>1533_bases
ATGAAAGAGCGTTTGGTTATTTTTGATACAACGTTGCGTGATGGTGAACAAAGTCCCGGTGCATCCATGACCATGGAAGA
GAAAGTGCGTATCGCTAGACAGTTGGAGCGCATGGGTGTGGATGTAATCGAAGCAGGTTTTCCAGCAGCATCAAAAGGTG
ACTTTGAGGCTGTCAAGGCAGTTGCGGAGGCCGTGAGCAACAGCACGGTATGCGGTCTTTCCCGTGCGATAGAAGCTGAT
ATCAATCGTGCCGGTGAAGCTCTGCAGGCAAATCAGAATATGCGCATCCACACCTTCATCGCCACCTCACCAATTCACAT
GAAGAACAAACTGCGCATGTCACCTGATCGGGTCATCGAACAAGCGATCAAGGCAGTAAAGTGGGCTCGCCAATATACGG
ATAACGTTGAATTTTCTCCTGAAGATGCCGGACGATCCGAAATAGATTTTCTTTGCCGGATTCTGGAAGCTGTAATTGAT
GCAGGTGCCCGGACGTTGAATATTCCGGATACAGTGGGATACACCATGCCGGATCAGTTTGGCGGTCTAATCCGCACGTT
ACGTGAACGAATTCCGAATTCTGACAAAGCTGTTTTCTCCGTTCATTGTCATAATGATCTCGGCCTGGCGGTAGCCAACT
CGCTATCTGCAGTCATGAATGGCGCGCGTCAGGTTGAATGCACCATCAATGGCTTGGGTGAACGCGCTGGCAATGCTGCT
CTTGAAGAAGTAGTGATGGCCGTGCGTACACGGCAAGATTACTTTCCATGCGATACACGCATTGATACAACCCAGATTGT
ATCTACTAGCAAGCTGGTTTCTGGAATTACCGGATTTCCGGTGCAACCCAACAAAGCTATTGTCGGTGCTAATGCTTTTG
CCCATGAATCAGGTATTCATCAGGATGGTGTACTCAAACATCGTGAGACCTATGAAATCATGCGCGCAGAAGATGTCGGC
TGGGGAGCGAACAAATTGTTGTTGGGCAAGCATTCCGGTCGTAATGCGTTTCGCAGTCGCTTGAAAGAACTGGGAATCGA
ATTGGAATCGGAAGAGAAACTGAATACCATATTTATCCGTTTCAAGGATCTTGCGGACAAAAAACACGAGATCTTTGATG
AAGATCTGCACGCGCTTGTTTCGGATGAGGCACAGATACCCGAAGAGCATTACCGCTTACTTTCACTAGTTGCACACAGC
GAGACCGGAGAAATACCTTTTGCACAGGTCGTTATTGCTACAGGAAATAACGAGCAACAGGCCGAATCAGAAGGAAGTGG
GCCGGTAGATGCGACGTTTCGTGCTATTGAAAAAATATTGAACAGTAAGGCAGAATTGCAATTGTTTTCTGTAAATAACA
TCACCAGCGGAACAGACGCTCTGGGTGAAGTAACTGTTCGATTGCAAAAAGTCGGACGAATCGTTAATGGCCATGGAGCA
GATACAGACATCATTGCAGCTTCTGCCAAAGCCTATCTGAATGCCTGTAATAAACTGCACTCCAGCCTGGAGCGCACACA
CCCACAGGTTTAG

Upstream 100 bases:

>100_bases
GGCGCTAATTATTCCAATCATTTTCCCATCTGTTTGAAGTGTAAAGGCTGGCATACCAGCCAACCGTCCGGTTTGAGCCA
GAATAATATGGAGTAGAACA

Downstream 100 bases:

>100_bases
ATCCGAAAATAATTAAAAATTGTCTACCATAAAGTTGATTTTATAAAAATAACCAGGGGGAAATTCCAATTCCGGTACAG
ATACAATTGCTGTACCGGAA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKERLVIFDTTLRDGEQSPGASMTMEEKVRIARQLERMGVDVIEAGFPAASKGDFEAVKAVAEAVSNSTVCGLSRAIEAD
INRAGEALQANQNMRIHTFIATSPIHMKNKLRMSPDRVIEQAIKAVKWARQYTDNVEFSPEDAGRSEIDFLCRILEAVID
AGARTLNIPDTVGYTMPDQFGGLIRTLRERIPNSDKAVFSVHCHNDLGLAVANSLSAVMNGARQVECTINGLGERAGNAA
LEEVVMAVRTRQDYFPCDTRIDTTQIVSTSKLVSGITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WGANKLLLGKHSGRNAFRSRLKELGIELESEEKLNTIFIRFKDLADKKHEIFDEDLHALVSDEAQIPEEHYRLLSLVAHS
ETGEIPFAQVVIATGNNEQQAESEGSGPVDATFRAIEKILNSKAELQLFSVNNITSGTDALGEVTVRLQKVGRIVNGHGA
DTDIIAASAKAYLNACNKLHSSLERTHPQV

Sequences:

>Translated_510_residues
MKERLVIFDTTLRDGEQSPGASMTMEEKVRIARQLERMGVDVIEAGFPAASKGDFEAVKAVAEAVSNSTVCGLSRAIEAD
INRAGEALQANQNMRIHTFIATSPIHMKNKLRMSPDRVIEQAIKAVKWARQYTDNVEFSPEDAGRSEIDFLCRILEAVID
AGARTLNIPDTVGYTMPDQFGGLIRTLRERIPNSDKAVFSVHCHNDLGLAVANSLSAVMNGARQVECTINGLGERAGNAA
LEEVVMAVRTRQDYFPCDTRIDTTQIVSTSKLVSGITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WGANKLLLGKHSGRNAFRSRLKELGIELESEEKLNTIFIRFKDLADKKHEIFDEDLHALVSDEAQIPEEHYRLLSLVAHS
ETGEIPFAQVVIATGNNEQQAESEGSGPVDATFRAIEKILNSKAELQLFSVNNITSGTDALGEVTVRLQKVGRIVNGHGA
DTDIIAASAKAYLNACNKLHSSLERTHPQV
>Mature_510_residues
MKERLVIFDTTLRDGEQSPGASMTMEEKVRIARQLERMGVDVIEAGFPAASKGDFEAVKAVAEAVSNSTVCGLSRAIEAD
INRAGEALQANQNMRIHTFIATSPIHMKNKLRMSPDRVIEQAIKAVKWARQYTDNVEFSPEDAGRSEIDFLCRILEAVID
AGARTLNIPDTVGYTMPDQFGGLIRTLRERIPNSDKAVFSVHCHNDLGLAVANSLSAVMNGARQVECTINGLGERAGNAA
LEEVVMAVRTRQDYFPCDTRIDTTQIVSTSKLVSGITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WGANKLLLGKHSGRNAFRSRLKELGIELESEEKLNTIFIRFKDLADKKHEIFDEDLHALVSDEAQIPEEHYRLLSLVAHS
ETGEIPFAQVVIATGNNEQQAESEGSGPVDATFRAIEKILNSKAELQLFSVNNITSGTDALGEVTVRLQKVGRIVNGHGA
DTDIIAASAKAYLNACNKLHSSLERTHPQV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=505, Percent_Identity=51.6831683168317, Blast_Score=497, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6324682, Length=539, Percent_Identity=25.7884972170686, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=554, Percent_Identity=25.4512635379061, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6320071, Length=382, Percent_Identity=27.7486910994764, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6320019, Length=382, Percent_Identity=27.4869109947644, Blast_Score=136, Evalue=9e-33,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_NITEC (Q0AGN5)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747462.1
- ProteinModelPortal:   Q0AGN5
- SMR:   Q0AGN5
- STRING:   Q0AGN5
- GeneID:   4272188
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1244
- NMPDR:   fig|335283.3.peg.1962
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   Q0AGN5
- ProtClustDB:   PRK00915
- BioCyc:   NEUT335283:NEUT_1244-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55836; Mature: 55836

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERLVIFDTTLRDGEQSPGASMTMEEKVRIARQLERMGVDVIEAGFPAASKGDFEAVKA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHH
VAEAVSNSTVCGLSRAIEADINRAGEALQANQNMRIHTFIATSPIHMKNKLRMSPDRVIE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHCCCCCHHHHHH
QAIKAVKWARQYTDNVEFSPEDAGRSEIDFLCRILEAVIDAGARTLNIPDTVGYTMPDQF
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHH
GGLIRTLRERIPNSDKAVFSVHCHNDLGLAVANSLSAVMNGARQVECTINGLGERAGNAA
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECHHHHCCHHH
LEEVVMAVRTRQDYFPCDTRIDTTQIVSTSKLVSGITGFPVQPNKAIVGANAFAHESGIH
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHCCCCC
QDGVLKHRETYEIMRAEDVGWGANKLLLGKHSGRNAFRSRLKELGIELESEEKLNTIFIR
CCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEE
FKDLADKKHEIFDEDLHALVSDEAQIPEEHYRLLSLVAHSETGEIPFAQVVIATGNNEQQ
EHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHH
AESEGSGPVDATFRAIEKILNSKAELQLFSVNNITSGTDALGEVTVRLQKVGRIVNGHGA
HHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DTDIIAASAKAYLNACNKLHSSLERTHPQV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKERLVIFDTTLRDGEQSPGASMTMEEKVRIARQLERMGVDVIEAGFPAASKGDFEAVKA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHH
VAEAVSNSTVCGLSRAIEADINRAGEALQANQNMRIHTFIATSPIHMKNKLRMSPDRVIE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHCCCCCHHHHHH
QAIKAVKWARQYTDNVEFSPEDAGRSEIDFLCRILEAVIDAGARTLNIPDTVGYTMPDQF
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHH
GGLIRTLRERIPNSDKAVFSVHCHNDLGLAVANSLSAVMNGARQVECTINGLGERAGNAA
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECHHHHCCHHH
LEEVVMAVRTRQDYFPCDTRIDTTQIVSTSKLVSGITGFPVQPNKAIVGANAFAHESGIH
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHCCCCC
QDGVLKHRETYEIMRAEDVGWGANKLLLGKHSGRNAFRSRLKELGIELESEEKLNTIFIR
CCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEE
FKDLADKKHEIFDEDLHALVSDEAQIPEEHYRLLSLVAHSETGEIPFAQVVIATGNNEQQ
EHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHH
AESEGSGPVDATFRAIEKILNSKAELQLFSVNNITSGTDALGEVTVRLQKVGRIVNGHGA
HHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DTDIIAASAKAYLNACNKLHSSLERTHPQV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA