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The map label for this gene is ksgA

Identifier: 114331217

GI number: 114331217

Start: 1277050

End: 1277820

Strand: Direct

Name: ksgA

Synonym: Neut_1219

Alternate gene names: 114331217

Gene position: 1277050-1277820 (Clockwise)

Preceding gene: 114331216

Following gene: 114331220

Centisome position: 47.99

GC content: 47.73

Gene sequence:

>771_bases
ATGCGGCATATTCCCCGCAGGCGTTTTGGCCAGCATTTTCTGGTTGATCATCACATCATCGCCGAGATCATTCACATCAT
TTGTCCACTACCAGGCGACCGAATGATTGAAATCGGGCCCGGATTGGGTGCATTAACCCAGCCTTTGCTGAATAATCTGG
ACACGCTGCAAGCCATTGAGCTCGACCGGGATATCGTTGATTATCTGTCCCGAAATTATGCTGAGAAGCTGGTCATTCAT
AATGTCGATGCTCTGAAATTTGATTTTTCTGCGCTGGGAGAGGGGCTGCGAATTGTTGGGAATCTGCCTTACAATATTTC
CACGCCACTATTGTTTCATCTGTCCCGATTCAGCAATCTGATTATCGATATGCACTTCATGTTGCAGCTTGAAGTGGTGG
AGCGCATGGTGGCACAGCCTTCCACGCCGGATTACGGGCGTTTGTCGCTGATGCTGCAAAACAGGTTCGAAATGGAACAA
ATGCTAATCGTCCCCGCAGAAGCGTTTAATCCACCTCCGCGAGTCCAGTCTGCCATTGTATGTATGCGCCCAAGAGTGGT
ACCAGTTATTCCATTTGGGCTGGAAAAACTGTTTGGTGAGATGGTCTTGGCTGCATTTTCTCAACGGCGTAAAACACTGC
GTAATACACTGCGTCATTATCTGACTATCAAGGATTTTGACCAGCTGAGGATTGATCCGGGGCTGCGGGCAGAAAATTTA
TCGCTTGAGCAATATTCAGCAATTACCCGCCACATCCATAAAATCAGATAA

Upstream 100 bases:

>100_bases
GATCCTGGCAGCATACATGCTGCCATTGAAATGGCGTTGACGCTCGTCAGGAACACCAGAAACAACTCTCTGCAGGCAAA
CCAACAATCTGAGAATTAAC

Downstream 100 bases:

>100_bases
GAGTAGTTTGCTTATCTTTATGTTCATGTTCAAGCAGCCACTCCTTGCGGTGGATACCGCCTCCGTACCCCGTTAGTTTG
CCGTTGGCGCCAATGACGCG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRHIPRRRFGQHFLVDHHIIAEIIHIICPLPGDRMIEIGPGLGALTQPLLNNLDTLQAIELDRDIVDYLSRNYAEKLVIH
NVDALKFDFSALGEGLRIVGNLPYNISTPLLFHLSRFSNLIIDMHFMLQLEVVERMVAQPSTPDYGRLSLMLQNRFEMEQ
MLIVPAEAFNPPPRVQSAIVCMRPRVVPVIPFGLEKLFGEMVLAAFSQRRKTLRNTLRHYLTIKDFDQLRIDPGLRAENL
SLEQYSAITRHIHKIR

Sequences:

>Translated_256_residues
MRHIPRRRFGQHFLVDHHIIAEIIHIICPLPGDRMIEIGPGLGALTQPLLNNLDTLQAIELDRDIVDYLSRNYAEKLVIH
NVDALKFDFSALGEGLRIVGNLPYNISTPLLFHLSRFSNLIIDMHFMLQLEVVERMVAQPSTPDYGRLSLMLQNRFEMEQ
MLIVPAEAFNPPPRVQSAIVCMRPRVVPVIPFGLEKLFGEMVLAAFSQRRKTLRNTLRHYLTIKDFDQLRIDPGLRAENL
SLEQYSAITRHIHKIR
>Mature_256_residues
MRHIPRRRFGQHFLVDHHIIAEIIHIICPLPGDRMIEIGPGLGALTQPLLNNLDTLQAIELDRDIVDYLSRNYAEKLVIH
NVDALKFDFSALGEGLRIVGNLPYNISTPLLFHLSRFSNLIIDMHFMLQLEVVERMVAQPSTPDYGRLSLMLQNRFEMEQ
MLIVPAEAFNPPPRVQSAIVCMRPRVVPVIPFGLEKLFGEMVLAAFSQRRKTLRNTLRHYLTIKDFDQLRIDPGLRAENL
SLEQYSAITRHIHKIR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=214, Percent_Identity=32.7102803738318, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI156415992, Length=277, Percent_Identity=28.5198555956679, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI1786236, Length=256, Percent_Identity=45.3125, Blast_Score=223, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI25146882, Length=220, Percent_Identity=31.3636363636364, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25141369, Length=278, Percent_Identity=23.3812949640288, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324989, Length=180, Percent_Identity=30, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358017, Length=214, Percent_Identity=29.9065420560748, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357273, Length=269, Percent_Identity=28.6245353159851, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_NITEC (Q0AGQ8)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747439.1
- ProteinModelPortal:   Q0AGQ8
- SMR:   Q0AGQ8
- STRING:   Q0AGQ8
- GeneID:   4271816
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1219
- NMPDR:   fig|335283.3.peg.1121
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   Q0AGQ8
- ProtClustDB:   PRK00274
- BioCyc:   NEUT335283:NEUT_1219-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 29528; Mature: 29528

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 12-12 BINDING 14-14 BINDING 39-39 BINDING 60-60 BINDING 83-83 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHIPRRRFGQHFLVDHHIIAEIIHIICPLPGDRMIEIGPGLGALTQPLLNNLDTLQAIE
CCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHH
LDRDIVDYLSRNYAEKLVIHNVDALKFDFSALGEGLRIVGNLPYNISTPLLFHLSRFSNL
HHHHHHHHHHHHHHHHHHHCCCHHHEECHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHH
IIDMHFMLQLEVVERMVAQPSTPDYGRLSLMLQNRFEMEQMLIVPAEAFNPPPRVQSAIV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEEHHHCCCCHHHHHHHH
CMRPRVVPVIPFGLEKLFGEMVLAAFSQRRKTLRNTLRHYLTIKDFDQLRIDPGLRAENL
HCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCC
SLEQYSAITRHIHKIR
CHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRHIPRRRFGQHFLVDHHIIAEIIHIICPLPGDRMIEIGPGLGALTQPLLNNLDTLQAIE
CCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHH
LDRDIVDYLSRNYAEKLVIHNVDALKFDFSALGEGLRIVGNLPYNISTPLLFHLSRFSNL
HHHHHHHHHHHHHHHHHHHCCCHHHEECHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHH
IIDMHFMLQLEVVERMVAQPSTPDYGRLSLMLQNRFEMEQMLIVPAEAFNPPPRVQSAIV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEEHHHCCCCHHHHHHHH
CMRPRVVPVIPFGLEKLFGEMVLAAFSQRRKTLRNTLRHYLTIKDFDQLRIDPGLRAENL
HCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCC
SLEQYSAITRHIHKIR
CHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA