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The map label for this gene is purD [H]

Identifier: 114331209

GI number: 114331209

Start: 1265945

End: 1267243

Strand: Direct

Name: purD [H]

Synonym: Neut_1211

Alternate gene names: 114331209

Gene position: 1265945-1267243 (Clockwise)

Preceding gene: 114331208

Following gene: 114331210

Centisome position: 47.57

GC content: 48.34

Gene sequence:

>1299_bases
ATGAAATTACTTGTGGTCGGCAGTGGCGGCAGAGAGCATGCTCTGGCATGGAAACTGGCTCAATCTCCATTGGTCAAGAA
GGTTTTTGTCGCACCTGGCAATGCCGGAACAGCACTGGAAGCCAGATTGGAAAATGTTCCGGTAACTTCTATTTCTGATT
TGCTGGCATTTGCCCGGCAGGAAAAAATTGATTTGACTATAGTAGGTCCAGAAGCGCCATTGACGGATGGTATCGTTGAT
ATCTTTCAGGCTGCGGGGCTGAAAATATTCGGCCCAACCCAAAATGCCGCACAGCTGGAAGGCTCCAAAAGCTTTGCCAA
AGCATTCATGCTTGGCCATGGTATTCCAACCGCTGCCGGTGAAACATTTAGCGATGCTGCTGCTGCCCATCACTATCTCG
ATCAGCATATTTTGCCTGTTGTCATCAAGGCTGACGGGTTGGCAGCCGGCAAGGGTGTGATTGTCGCGCAGACATGGGAT
GAAGCACATGCAGCTGTCGATTCCATGCTGGTAGATAATAAATTAGGGGCTGCAGGGGCACATATCTTGATCGAAGAATA
TCTGGAAGGGGAGGAAGCCAGCTTTATTGTCTTGTCGGATGGACAGAATGTTGTGCCTCTGGCTACCAGTCAGGACCACA
AGCGTTTGTTGGATAATGATAAAGGGCCAAACACGGGTGGCATGGGTGCCTATTCTCCGGCATCGGTTATTACTCCTGGC
CTGCATGAAAAAATAATGCGGGAAGTCATCATGCCCGTGATCGAAGGGATGGCACAACAATCACAACCTTATACCGGATT
TCTTTATGCGGGATTGATGATTTCATCTCAGGGTGATATCAGAGTGCTAGAATTTAACTGTCGCTTAGGTGATCCGGAAA
CCCAAGCCATCATGCTGCGACTGAAAACTGATTTATTTTCTTTGCTGGATCATGCGATCAATGGCACACTGGATAAAGTC
AATGTTGAGTGGGATCCTAGAGTTGCATTATGTGTCGTGATGGCGGCACAAGGCTATCCTGATAGCCCACGGAAAAATGA
TGTTATTCACGGCTTGGATGTAATGATGGCGAATCAGGATGCGGATGATAAATTTCATATTTTTCATGCCGGAACTACTG
CAAGTAGCGGAAATCCAGAGAAAATCCTGACTGCCGGGGGACGGGTGCTGGGAGTGACTGCTTTAGGTGAAGACATCCAG
CAGGCACGGGCGCGCGCTTATGCCATTGCAAAGAGCATTCATTTCGATGGCTGTCAGATGCGCCGGGATATTGGGTATAG
GGGATTAAGAAATGAATGA

Upstream 100 bases:

>100_bases
CATGATCTTTACTGGTGTCAGACATTTCAGACATTAGCAGATTACGTTTCTTTTTTGCTTCCCGTCAACTTGTACCCTTA
AATTCTATTCAGATAGTCAG

Downstream 100 bases:

>100_bases
TACAACAACAAAATTGTTGCTGGCCGCATCGTTTGCGTTGCTGCTATCTGTTTAGTCCCACGGTGTGCCGGTGTAACATA
TAACCAGCTACGGAGGGATG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKLLVVGSGGREHALAWKLAQSPLVKKVFVAPGNAGTALEARLENVPVTSISDLLAFARQEKIDLTIVGPEAPLTDGIVD
IFQAAGLKIFGPTQNAAQLEGSKSFAKAFMLGHGIPTAAGETFSDAAAAHHYLDQHILPVVIKADGLAAGKGVIVAQTWD
EAHAAVDSMLVDNKLGAAGAHILIEEYLEGEEASFIVLSDGQNVVPLATSQDHKRLLDNDKGPNTGGMGAYSPASVITPG
LHEKIMREVIMPVIEGMAQQSQPYTGFLYAGLMISSQGDIRVLEFNCRLGDPETQAIMLRLKTDLFSLLDHAINGTLDKV
NVEWDPRVALCVVMAAQGYPDSPRKNDVIHGLDVMMANQDADDKFHIFHAGTTASSGNPEKILTAGGRVLGVTALGEDIQ
QARARAYAIAKSIHFDGCQMRRDIGYRGLRNE

Sequences:

>Translated_432_residues
MKLLVVGSGGREHALAWKLAQSPLVKKVFVAPGNAGTALEARLENVPVTSISDLLAFARQEKIDLTIVGPEAPLTDGIVD
IFQAAGLKIFGPTQNAAQLEGSKSFAKAFMLGHGIPTAAGETFSDAAAAHHYLDQHILPVVIKADGLAAGKGVIVAQTWD
EAHAAVDSMLVDNKLGAAGAHILIEEYLEGEEASFIVLSDGQNVVPLATSQDHKRLLDNDKGPNTGGMGAYSPASVITPG
LHEKIMREVIMPVIEGMAQQSQPYTGFLYAGLMISSQGDIRVLEFNCRLGDPETQAIMLRLKTDLFSLLDHAINGTLDKV
NVEWDPRVALCVVMAAQGYPDSPRKNDVIHGLDVMMANQDADDKFHIFHAGTTASSGNPEKILTAGGRVLGVTALGEDIQ
QARARAYAIAKSIHFDGCQMRRDIGYRGLRNE
>Mature_432_residues
MKLLVVGSGGREHALAWKLAQSPLVKKVFVAPGNAGTALEARLENVPVTSISDLLAFARQEKIDLTIVGPEAPLTDGIVD
IFQAAGLKIFGPTQNAAQLEGSKSFAKAFMLGHGIPTAAGETFSDAAAAHHYLDQHILPVVIKADGLAAGKGVIVAQTWD
EAHAAVDSMLVDNKLGAAGAHILIEEYLEGEEASFIVLSDGQNVVPLATSQDHKRLLDNDKGPNTGGMGAYSPASVITPG
LHEKIMREVIMPVIEGMAQQSQPYTGFLYAGLMISSQGDIRVLEFNCRLGDPETQAIMLRLKTDLFSLLDHAINGTLDKV
NVEWDPRVALCVVMAAQGYPDSPRKNDVIHGLDVMMANQDADDKFHIFHAGTTASSGNPEKILTAGGRVLGVTALGEDIQ
QARARAYAIAKSIHFDGCQMRRDIGYRGLRNE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=433, Percent_Identity=46.8822170900693, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI4503915, Length=433, Percent_Identity=46.8822170900693, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI209869995, Length=433, Percent_Identity=46.8822170900693, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI209869993, Length=433, Percent_Identity=46.8822170900693, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1790438, Length=434, Percent_Identity=59.9078341013825, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17567511, Length=430, Percent_Identity=41.6279069767442, Blast_Score=303, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6321203, Length=449, Percent_Identity=42.5389755011136, Blast_Score=333, Evalue=3e-92,
Organism=Drosophila melanogaster, GI62471619, Length=438, Percent_Identity=43.37899543379, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24582400, Length=438, Percent_Identity=43.37899543379, Blast_Score=317, Evalue=7e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46130; Mature: 46130

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVVGSGGREHALAWKLAQSPLVKKVFVAPGNAGTALEARLENVPVTSISDLLAFARQ
CEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHH
EKIDLTIVGPEAPLTDGIVDIFQAAGLKIFGPTQNAAQLEGSKSFAKAFMLGHGIPTAAG
CCEEEEEECCCCCCHHHHHHHHHHCCEEEECCCCCHHHCCCCHHHHHHHHHCCCCCCCCC
ETFSDAAAAHHYLDQHILPVVIKADGLAAGKGVIVAQTWDEAHAAVDSMLVDNKLGAAGA
CCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCHH
HILIEEYLEGEEASFIVLSDGQNVVPLATSQDHKRLLDNDKGPNTGGMGAYSPASVITPG
HHHHHHHHCCCCCEEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCC
LHEKIMREVIMPVIEGMAQQSQPYTGFLYAGLMISSQGDIRVLEFNCRLGDPETQAIMLR
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCEEEEEEEECCCCCCCCEEEEE
LKTDLFSLLDHAINGTLDKVNVEWDPRVALCVVMAAQGYPDSPRKNDVIHGLDVMMANQD
HHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHCCCCCCCCCCCCCCCEEEEEECCC
ADDKFHIFHAGTTASSGNPEKILTAGGRVLGVTALGEDIQQARARAYAIAKSIHFDGCQM
CCCCEEEEECCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHH
RRDIGYRGLRNE
HHHCCCCCCCCC
>Mature Secondary Structure
MKLLVVGSGGREHALAWKLAQSPLVKKVFVAPGNAGTALEARLENVPVTSISDLLAFARQ
CEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHH
EKIDLTIVGPEAPLTDGIVDIFQAAGLKIFGPTQNAAQLEGSKSFAKAFMLGHGIPTAAG
CCEEEEEECCCCCCHHHHHHHHHHCCEEEECCCCCHHHCCCCHHHHHHHHHCCCCCCCCC
ETFSDAAAAHHYLDQHILPVVIKADGLAAGKGVIVAQTWDEAHAAVDSMLVDNKLGAAGA
CCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCHH
HILIEEYLEGEEASFIVLSDGQNVVPLATSQDHKRLLDNDKGPNTGGMGAYSPASVITPG
HHHHHHHHCCCCCEEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCC
LHEKIMREVIMPVIEGMAQQSQPYTGFLYAGLMISSQGDIRVLEFNCRLGDPETQAIMLR
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCEEEEEEEECCCCCCCCEEEEE
LKTDLFSLLDHAINGTLDKVNVEWDPRVALCVVMAAQGYPDSPRKNDVIHGLDVMMANQD
HHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHCCCCCCCCCCCCCCCEEEEEECCC
ADDKFHIFHAGTTASSGNPEKILTAGGRVLGVTALGEDIQQARARAYAIAKSIHFDGCQM
CCCCEEEEECCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHH
RRDIGYRGLRNE
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]