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The map label for this gene is purH [H]

Identifier: 114331208

GI number: 114331208

Start: 1264319

End: 1265881

Strand: Direct

Name: purH [H]

Synonym: Neut_1210

Alternate gene names: 114331208

Gene position: 1264319-1265881 (Clockwise)

Preceding gene: 114331207

Following gene: 114331209

Centisome position: 47.51

GC content: 47.79

Gene sequence:

>1563_bases
ATGAGTATTAAACAAGCACTCATCAGTGTTTCAGACAAAACAGGCGTGGTTGAGCTGGCCTCGGCTCTGCATCAACTCGA
TATTATGATTCTTTCAACCGGCGGGACTGCAAAATTGCTGCAGGAAGCGAGTATTCCCGTCATAGAAGTGAGCGACTATA
CTGGCTTCCCTGAAATGCTGGATGGACGGGTTAAGACGCTGCATCCCAAGGTACACGCAGGGATCCTTGCCAGGATGGAT
TTGCCTGCACATCAGCAGGCAATGGCACAGGCACAGATTCCAGCGATTGATCTGATAGTGGTTAATCTGTATCCGTTTAC
CAGTACAATCGCCCGGCAAGACTGTACAGTTGAAGAGGCAATCGAGAACATTGATATCGGTGGTCCTACTATGATCCGGG
CAGCTGCCAAGAATTTTCAGCGGGTTACCGTTGTGACCGACCCGCAGGATTATGAACCACTACTGGAACAAATACGATCA
AATAATGGATCAGTTGATCATGATTATCGTTTCAAGTTAGCGCAAAAGGCTTTTTCGCATACTGCACGCTACGATGGGGC
AATCAGCAATTATTTGACGGCACTGGAATCCTCAACCGGCCAGCGCAAACCATTTCCGGATCAATTCAATCTCAGTTTCA
CATTAACGCAATCTTTGCGTTATGGAGAAAATCCTCATCAGCAGGCAGCGTTTTATCGTGATGCAGCAGATATAACAGGC
AGTCTGGCAGACTATAACCAGCTCCAGGGGAAGGCACTGTCCTATAACAATATTGCTGATACCGATGCGGCATTAGCATG
TGTCAGAACTTTCGATAAACCCGCCTGCGTTATCATTAAGCATGCCAATCCTTGTGGCGTCGCCATTGCTGATAATCCGC
TTGCTGCTTACAAGAAAGCATTTACTACCGACCCGGTATCAGCCTTTGGCGGCATTATCGCGATTAATCGCGCAGTCGAA
GCTGATCTGGCTAAAGCAGTGTTGCAACAATTTGTGGAAGTGATTATTGCACCGGAATTCAGCATGGAAGCCAGACAACT
TCTGGCAGGAAAATCCAATATTCGGGTACTGACCGTTTCATTGGGCGCGCATGGCAATCTGTATGATCTGAAGCGTGTGG
ATGGTGGTTTACTGGTTCAAACACCGGACGATCTTAATCTCACGGCTAATCAGTTACGAGTGGTGACACAAGTAAAGCCA
ACGGATGCACAAATTGAGGATTGCCTATTTGCATGGCGAGTGGCAAAGTTTGTCAAATCCAACACCATTGTATTTTGTAG
CAATGGCCAAACACTTGGCATTGGTGCCGGACAGATGAGTCGGGTGGACAGTGCCCGTATTGCTTCAATTAAGGCAGAAC
AGGTTCATCTTGATTTGCATGGATCTGTCGTTGCATCGGATGCATTTTTTCCGTTTCGGGATGGCCTGGATGTGCTCGCC
CAGGCAGGTGCCAAGGCTGTCATCCAACCAGGTGGCAGTATTCGCGATGAAGAGGTCATTGCTGCTGCCAACGAACAAGG
GATAGCCATGATCTTTACTGGTGTCAGACATTTCAGACATTAG

Upstream 100 bases:

>100_bases
TGCACAAGGCAATCAAAGCAAGGCGGCTGAACTACTTGGCATCAATCGTAATACCTTGCGTAACCGGCTAACCAAACATC
AGATCGGATAATTTTTTCAA

Downstream 100 bases:

>100_bases
CAGATTACGTTTCTTTTTTGCTTCCCGTCAACTTGTACCCTTAAATTCTATTCAGATAGTCAGATGAAATTACTTGTGGT
CGGCAGTGGCGGCAGAGAGC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSIKQALISVSDKTGVVELASALHQLDIMILSTGGTAKLLQEASIPVIEVSDYTGFPEMLDGRVKTLHPKVHAGILARMD
LPAHQQAMAQAQIPAIDLIVVNLYPFTSTIARQDCTVEEAIENIDIGGPTMIRAAAKNFQRVTVVTDPQDYEPLLEQIRS
NNGSVDHDYRFKLAQKAFSHTARYDGAISNYLTALESSTGQRKPFPDQFNLSFTLTQSLRYGENPHQQAAFYRDAADITG
SLADYNQLQGKALSYNNIADTDAALACVRTFDKPACVIIKHANPCGVAIADNPLAAYKKAFTTDPVSAFGGIIAINRAVE
ADLAKAVLQQFVEVIIAPEFSMEARQLLAGKSNIRVLTVSLGAHGNLYDLKRVDGGLLVQTPDDLNLTANQLRVVTQVKP
TDAQIEDCLFAWRVAKFVKSNTIVFCSNGQTLGIGAGQMSRVDSARIASIKAEQVHLDLHGSVVASDAFFPFRDGLDVLA
QAGAKAVIQPGGSIRDEEVIAAANEQGIAMIFTGVRHFRH

Sequences:

>Translated_520_residues
MSIKQALISVSDKTGVVELASALHQLDIMILSTGGTAKLLQEASIPVIEVSDYTGFPEMLDGRVKTLHPKVHAGILARMD
LPAHQQAMAQAQIPAIDLIVVNLYPFTSTIARQDCTVEEAIENIDIGGPTMIRAAAKNFQRVTVVTDPQDYEPLLEQIRS
NNGSVDHDYRFKLAQKAFSHTARYDGAISNYLTALESSTGQRKPFPDQFNLSFTLTQSLRYGENPHQQAAFYRDAADITG
SLADYNQLQGKALSYNNIADTDAALACVRTFDKPACVIIKHANPCGVAIADNPLAAYKKAFTTDPVSAFGGIIAINRAVE
ADLAKAVLQQFVEVIIAPEFSMEARQLLAGKSNIRVLTVSLGAHGNLYDLKRVDGGLLVQTPDDLNLTANQLRVVTQVKP
TDAQIEDCLFAWRVAKFVKSNTIVFCSNGQTLGIGAGQMSRVDSARIASIKAEQVHLDLHGSVVASDAFFPFRDGLDVLA
QAGAKAVIQPGGSIRDEEVIAAANEQGIAMIFTGVRHFRH
>Mature_519_residues
SIKQALISVSDKTGVVELASALHQLDIMILSTGGTAKLLQEASIPVIEVSDYTGFPEMLDGRVKTLHPKVHAGILARMDL
PAHQQAMAQAQIPAIDLIVVNLYPFTSTIARQDCTVEEAIENIDIGGPTMIRAAAKNFQRVTVVTDPQDYEPLLEQIRSN
NGSVDHDYRFKLAQKAFSHTARYDGAISNYLTALESSTGQRKPFPDQFNLSFTLTQSLRYGENPHQQAAFYRDAADITGS
LADYNQLQGKALSYNNIADTDAALACVRTFDKPACVIIKHANPCGVAIADNPLAAYKKAFTTDPVSAFGGIIAINRAVEA
DLAKAVLQQFVEVIIAPEFSMEARQLLAGKSNIRVLTVSLGAHGNLYDLKRVDGGLLVQTPDDLNLTANQLRVVTQVKPT
DAQIEDCLFAWRVAKFVKSNTIVFCSNGQTLGIGAGQMSRVDSARIASIKAEQVHLDLHGSVVASDAFFPFRDGLDVLAQ
AGAKAVIQPGGSIRDEEVIAAANEQGIAMIFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=494, Percent_Identity=37.0445344129555, Blast_Score=257, Evalue=2e-68,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=57.3333333333333, Blast_Score=593, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI71985564, Length=518, Percent_Identity=34.1698841698842, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI71985574, Length=366, Percent_Identity=26.2295081967213, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=50, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323768, Length=492, Percent_Identity=35.5691056910569, Blast_Score=266, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6323056, Length=492, Percent_Identity=35.3658536585366, Blast_Score=266, Evalue=9e-72,
Organism=Drosophila melanogaster, GI24649832, Length=491, Percent_Identity=36.4562118126273, Blast_Score=251, Evalue=9e-67,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56245; Mature: 56113

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKQALISVSDKTGVVELASALHQLDIMILSTGGTAKLLQEASIPVIEVSDYTGFPEML
CCHHHHHHHCCCCCCHHHHHHHHHHEEEEEEECCCHHHHHHHCCCCEEEECCCCCCHHHH
DGRVKTLHPKVHAGILARMDLPAHQQAMAQAQIPAIDLIVVNLYPFTSTIARQDCTVEEA
CCCCEEECCHHHHCEEEECCCCHHHHHHHHHCCCEEEEEEEEEECCHHHHHHCCCCHHHH
IENIDIGGPTMIRAAAKNFQRVTVVTDPQDYEPLLEQIRSNNGSVDHDYRFKLAQKAFSH
HHHCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
TARYDGAISNYLTALESSTGQRKPFPDQFNLSFTLTQSLRYGENPHQQAAFYRDAADITG
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHCC
SLADYNQLQGKALSYNNIADTDAALACVRTFDKPACVIIKHANPCGVAIADNPLAAYKKA
CHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHHHHHH
FTTDPVSAFGGIIAINRAVEADLAKAVLQQFVEVIIAPEFSMEARQLLAGKSNIRVLTVS
CCCCCHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEE
LGAHGNLYDLKRVDGGLLVQTPDDLNLTANQLRVVTQVKPTDAQIEDCLFAWRVAKFVKS
ECCCCCEEEHEECCCCEEEECCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHCC
NTIVFCSNGQTLGIGAGQMSRVDSARIASIKAEQVHLDLHGSVVASDAFFPFRDGLDVLA
CEEEEECCCCEEEECCCHHHHCCCHHHHEEEEEEEEEEECCCEEECCCCCCCCCCHHHHH
QAGAKAVIQPGGSIRDEEVIAAANEQGIAMIFTGVRHFRH
HCCCCEEECCCCCCCCCCEEEECCCCCEEEEEEHHHHHCC
>Mature Secondary Structure 
SIKQALISVSDKTGVVELASALHQLDIMILSTGGTAKLLQEASIPVIEVSDYTGFPEML
CHHHHHHHCCCCCCHHHHHHHHHHEEEEEEECCCHHHHHHHCCCCEEEECCCCCCHHHH
DGRVKTLHPKVHAGILARMDLPAHQQAMAQAQIPAIDLIVVNLYPFTSTIARQDCTVEEA
CCCCEEECCHHHHCEEEECCCCHHHHHHHHHCCCEEEEEEEEEECCHHHHHHCCCCHHHH
IENIDIGGPTMIRAAAKNFQRVTVVTDPQDYEPLLEQIRSNNGSVDHDYRFKLAQKAFSH
HHHCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
TARYDGAISNYLTALESSTGQRKPFPDQFNLSFTLTQSLRYGENPHQQAAFYRDAADITG
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHCC
SLADYNQLQGKALSYNNIADTDAALACVRTFDKPACVIIKHANPCGVAIADNPLAAYKKA
CHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHHHHHH
FTTDPVSAFGGIIAINRAVEADLAKAVLQQFVEVIIAPEFSMEARQLLAGKSNIRVLTVS
CCCCCHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEE
LGAHGNLYDLKRVDGGLLVQTPDDLNLTANQLRVVTQVKPTDAQIEDCLFAWRVAKFVKS
ECCCCCEEEHEECCCCEEEECCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHCC
NTIVFCSNGQTLGIGAGQMSRVDSARIASIKAEQVHLDLHGSVVASDAFFPFRDGLDVLA
CEEEEECCCCEEEECCCHHHHCCCHHHHEEEEEEEEEEECCCEEECCCCCCCCCCHHHHH
QAGAKAVIQPGGSIRDEEVIAAANEQGIAMIFTGVRHFRH
HCCCCEEECCCCCCCCCCEEEECCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA