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The map label for this gene is leuB [H]

Identifier: 114331145

GI number: 114331145

Start: 1185514

End: 1186584

Strand: Direct

Name: leuB [H]

Synonym: Neut_1147

Alternate gene names: 114331145

Gene position: 1185514-1186584 (Clockwise)

Preceding gene: 114331144

Following gene: 114331146

Centisome position: 44.55

GC content: 46.41

Gene sequence:

>1071_bases
ATGAAAATTGCATTGTTGGCAGGGGATGGTATTGGCCCTGAAATTGTGGCACAAGCCGAGCGCGTGCTGCTTTATTTTAA
ACAAGAAGGATTAAAAATCGAGCTGGAGCCCGGTTTACTGGGTGGTTGTGCAGTAGATGCAACAGGAAAGCCTTTTCCGG
AGGCAACACGTAAACTGTCTGTTGAAGCCGATGCCATATTGCTTGGGGCAGTGGGCGGAATTCAATACGACAATCTGCCT
CGCGAAAAACGGCCAGAGCAAGGGCTGCTGGCTATTCGTAAGACACTTGGCCTGTTCGCCAATCTGCGGCCGATAGTACT
CTATCCCGAGCTGGTAAATGCATCGACTCTGAAACCGGAGATTGTGACCGGGTTAGATATTTTGATTGTACGTGAATTAA
CCGGTGATATTTACTTCGGAAAACCGCGAGGTATCGAATACAGAGATGGGCAGAAAGTGGGATTTAATACAATGATTTAT
TCTGAATCAGAAATCAGGCGTATTGCTCATGTTGCATTTCAGGCAGCACGCAGGCGAAGTGGCAGATTATGCTCGGTAGA
TAAAATGAACGTTCTGGAATGTACGCAATTATGGCGTGATGTGGTAACAGAAACTGCACAATCCTATCCGGATGTTACGC
TCTCGCATATGCTGGTGGATAATGCAGCAATGCAACTGGTACGTAACCCTAAACAATTTGATGTAATCGTTACGGGCAAT
ATGTTTGGCGATATTTTATCAGACGAAGCATCCATGCTAACGGGTTCAATTGGAATGCTTCCGTCAGCCTCTCTGGATGA
CCGAAACAGGGGTCTGTATGAGCCTATTCATGGATCTGCGCCGGATATAGCCGGTAAAAATATTGCCAATCCACTGGCGA
CTATTTTGTCCGCAGCGATGATGCTGCGCTATTCTTTTAATGAGGAGACAGCTGCATCACGAATTGAAAGCGCTGTTCAG
AAAACACTGAAACAAGGTTATCGCACCGAGGACATTTATGAAACAGGCTTAAGGAGAACAGGTACCGTGGAAATGGGGGA
TATGGTCCTGGCAAATTTGCAATCCCAGTGA

Upstream 100 bases:

>100_bases
ACTCTGCAGCATGCCGACAGAATCAGAAAATTTGAGATAAATCGAAGAAATGAGCAGCCTTGGCTATTTTTGTAACAAGA
AATGAAGGCAGGTTGAGTGT

Downstream 100 bases:

>100_bases
TCTTTCATTTCTGTTTATGAATCTGCCTGTTTTACTTTTTTCTTCTGATTTTTGGATAATACGCCGGGTAGCAAGCTGTC
GAGTACAGTGCAATATTAAA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKIALLAGDGIGPEIVAQAERVLLYFKQEGLKIELEPGLLGGCAVDATGKPFPEATRKLSVEADAILLGAVGGIQYDNLP
REKRPEQGLLAIRKTLGLFANLRPIVLYPELVNASTLKPEIVTGLDILIVRELTGDIYFGKPRGIEYRDGQKVGFNTMIY
SESEIRRIAHVAFQAARRRSGRLCSVDKMNVLECTQLWRDVVTETAQSYPDVTLSHMLVDNAAMQLVRNPKQFDVIVTGN
MFGDILSDEASMLTGSIGMLPSASLDDRNRGLYEPIHGSAPDIAGKNIANPLATILSAAMMLRYSFNEETAASRIESAVQ
KTLKQGYRTEDIYETGLRRTGTVEMGDMVLANLQSQ

Sequences:

>Translated_356_residues
MKIALLAGDGIGPEIVAQAERVLLYFKQEGLKIELEPGLLGGCAVDATGKPFPEATRKLSVEADAILLGAVGGIQYDNLP
REKRPEQGLLAIRKTLGLFANLRPIVLYPELVNASTLKPEIVTGLDILIVRELTGDIYFGKPRGIEYRDGQKVGFNTMIY
SESEIRRIAHVAFQAARRRSGRLCSVDKMNVLECTQLWRDVVTETAQSYPDVTLSHMLVDNAAMQLVRNPKQFDVIVTGN
MFGDILSDEASMLTGSIGMLPSASLDDRNRGLYEPIHGSAPDIAGKNIANPLATILSAAMMLRYSFNEETAASRIESAVQ
KTLKQGYRTEDIYETGLRRTGTVEMGDMVLANLQSQ
>Mature_356_residues
MKIALLAGDGIGPEIVAQAERVLLYFKQEGLKIELEPGLLGGCAVDATGKPFPEATRKLSVEADAILLGAVGGIQYDNLP
REKRPEQGLLAIRKTLGLFANLRPIVLYPELVNASTLKPEIVTGLDILIVRELTGDIYFGKPRGIEYRDGQKVGFNTMIY
SESEIRRIAHVAFQAARRRSGRLCSVDKMNVLECTQLWRDVVTETAQSYPDVTLSHMLVDNAAMQLVRNPKQFDVIVTGN
MFGDILSDEASMLTGSIGMLPSASLDDRNRGLYEPIHGSAPDIAGKNIANPLATILSAAMMLRYSFNEETAASRIESAVQ
KTLKQGYRTEDIYETGLRRTGTVEMGDMVLANLQSQ

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=32.2388059701493, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI28178821, Length=320, Percent_Identity=29.0625, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI28178816, Length=320, Percent_Identity=29.0625, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI4758582, Length=340, Percent_Identity=29.7058823529412, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI28178838, Length=330, Percent_Identity=29.6969696969697, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178819, Length=162, Percent_Identity=35.1851851851852, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87081683, Length=351, Percent_Identity=50.4273504273504, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1788101, Length=342, Percent_Identity=33.3333333333333, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787381, Length=309, Percent_Identity=26.8608414239482, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=349, Percent_Identity=31.8051575931232, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17505779, Length=358, Percent_Identity=28.4916201117318, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=364, Percent_Identity=29.9450549450549, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=330, Percent_Identity=29.0909090909091, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=46.1111111111111, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6322097, Length=348, Percent_Identity=36.4942528735632, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=339, Percent_Identity=30.9734513274336, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324709, Length=344, Percent_Identity=32.2674418604651, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24643268, Length=335, Percent_Identity=34.0298507462687, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24643270, Length=335, Percent_Identity=34.0298507462687, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24661184, Length=349, Percent_Identity=29.7994269340974, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161078635, Length=338, Percent_Identity=26.0355029585799, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI161078637, Length=338, Percent_Identity=26.0355029585799, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI161078633, Length=338, Percent_Identity=26.0355029585799, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24650122, Length=338, Percent_Identity=26.0355029585799, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI161078639, Length=336, Percent_Identity=26.1904761904762, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281362242, Length=338, Percent_Identity=28.1065088757396, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24648872, Length=338, Percent_Identity=28.1065088757396, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI20130355, Length=336, Percent_Identity=23.2142857142857, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39005; Mature: 39005

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALLAGDGIGPEIVAQAERVLLYFKQEGLKIELEPGLLGGCAVDATGKPFPEATRKLS
CEEEEEECCCCCHHHHHHHHHHEEEEECCCCEEEECCCEECCEEECCCCCCCHHHHHEEC
VEADAILLGAVGGIQYDNLPREKRPEQGLLAIRKTLGLFANLRPIVLYPELVNASTLKPE
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCH
IVTGLDILIVRELTGDIYFGKPRGIEYRDGQKVGFNTMIYSESEIRRIAHVAFQAARRRS
HHCCHHHEEEEECCCCEEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCC
GRLCSVDKMNVLECTQLWRDVVTETAQSYPDVTLSHMLVDNAAMQLVRNPKQFDVIVTGN
CCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECC
MFGDILSDEASMLTGSIGMLPSASLDDRNRGLYEPIHGSAPDIAGKNIANPLATILSAAM
HHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
MLRYSFNEETAASRIESAVQKTLKQGYRTEDIYETGLRRTGTVEMGDMVLANLQSQ
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEHHHHHHHHCCCC
>Mature Secondary Structure
MKIALLAGDGIGPEIVAQAERVLLYFKQEGLKIELEPGLLGGCAVDATGKPFPEATRKLS
CEEEEEECCCCCHHHHHHHHHHEEEEECCCCEEEECCCEECCEEECCCCCCCHHHHHEEC
VEADAILLGAVGGIQYDNLPREKRPEQGLLAIRKTLGLFANLRPIVLYPELVNASTLKPE
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCH
IVTGLDILIVRELTGDIYFGKPRGIEYRDGQKVGFNTMIYSESEIRRIAHVAFQAARRRS
HHCCHHHEEEEECCCCEEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCC
GRLCSVDKMNVLECTQLWRDVVTETAQSYPDVTLSHMLVDNAAMQLVRNPKQFDVIVTGN
CCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECC
MFGDILSDEASMLTGSIGMLPSASLDDRNRGLYEPIHGSAPDIAGKNIANPLATILSAAM
HHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
MLRYSFNEETAASRIESAVQKTLKQGYRTEDIYETGLRRTGTVEMGDMVLANLQSQ
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]