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The map label for this gene is leuD

Identifier: 114331144

GI number: 114331144

Start: 1184850

End: 1185488

Strand: Direct

Name: leuD

Synonym: Neut_1146

Alternate gene names: 114331144

Gene position: 1184850-1185488 (Clockwise)

Preceding gene: 114331143

Following gene: 114331145

Centisome position: 44.53

GC content: 42.72

Gene sequence:

>639_bases
ATGAAAAAATTTGAACAGCTTCGGGGGATAGTTGCACCGCTGGATCGTGCAAATATTGATACAGACGCGATCATTCCCAA
GCAATTTCTGAAATCAATCAAGCGTTCAGGTTTTGGCCAGAACCTGTTCGATGAATGGCGTTATCTTGATTATGGTGAAC
CGGGAAAAGATGCTGCATCGCGTCAACTTAATCCGGATTTCATACTTAACCAATCACGCTATCAAGGGGCTAAAATTCTT
GTTACACGCGATAACTTTGGCTGCGGATCCAGCAGAGAACACGCTCCCTGGGCTTTGCAGGATTACGGCTTCGCTGTAAT
TATTGCGCCAAGTTTTGCTGATATTTTTTACAATAACTGTTTCAAGATTGGTCTGTTGCCAATTGTACTGGAGTCACCTG
TTGTTGACAGATTGATCAGGGATGCTCTGACAGTGGATGGTTATCAACTGGAAATTAATCTGGAAGCACAAACCGTGATG
GCTCTATCCGGTGAGACTTACTGGTTTGAGGTTGATCATTTCCGTAAACATTGCTTACTTAACGGGTTGGATGAGATTGG
TTTAACTCTGCAGCATGCCGACAGAATCAGAAAATTTGAGATAAATCGAAGAAATGAGCAGCCTTGGCTATTTTTGTAA

Upstream 100 bases:

>100_bases
TAATTACTGCGCAGCTGACTGCTTGTAATACGATGCAAGGATTTGGTAAGGATGTTCAGCGTGGTGGCGAAGCCATTGAA
AGAACTGCTAAATAAATGGA

Downstream 100 bases:

>100_bases
CAAGAAATGAAGGCAGGTTGAGTGTATGAAAATTGCATTGTTGGCAGGGGATGGTATTGGCCCTGAAATTGTGGCACAAG
CCGAGCGCGTGCTGCTTTAT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKKFEQLRGIVAPLDRANIDTDAIIPKQFLKSIKRSGFGQNLFDEWRYLDYGEPGKDAASRQLNPDFILNQSRYQGAKIL
VTRDNFGCGSSREHAPWALQDYGFAVIIAPSFADIFYNNCFKIGLLPIVLESPVVDRLIRDALTVDGYQLEINLEAQTVM
ALSGETYWFEVDHFRKHCLLNGLDEIGLTLQHADRIRKFEINRRNEQPWLFL

Sequences:

>Translated_212_residues
MKKFEQLRGIVAPLDRANIDTDAIIPKQFLKSIKRSGFGQNLFDEWRYLDYGEPGKDAASRQLNPDFILNQSRYQGAKIL
VTRDNFGCGSSREHAPWALQDYGFAVIIAPSFADIFYNNCFKIGLLPIVLESPVVDRLIRDALTVDGYQLEINLEAQTVM
ALSGETYWFEVDHFRKHCLLNGLDEIGLTLQHADRIRKFEINRRNEQPWLFL
>Mature_212_residues
MKKFEQLRGIVAPLDRANIDTDAIIPKQFLKSIKRSGFGQNLFDEWRYLDYGEPGKDAASRQLNPDFILNQSRYQGAKIL
VTRDNFGCGSSREHAPWALQDYGFAVIIAPSFADIFYNNCFKIGLLPIVLESPVVDRLIRDALTVDGYQLEINLEAQTVM
ALSGETYWFEVDHFRKHCLLNGLDEIGLTLQHADRIRKFEINRRNEQPWLFL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=202, Percent_Identity=56.4356435643564, Blast_Score=229, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6321429, Length=214, Percent_Identity=51.8691588785047, Blast_Score=207, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_NITEC (Q0AGY1)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747366.1
- ProteinModelPortal:   Q0AGY1
- SMR:   Q0AGY1
- STRING:   Q0AGY1
- GeneID:   4274112
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1146
- NMPDR:   fig|335283.3.peg.971
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q0AGY1
- ProtClustDB:   PRK01641
- BioCyc:   NEUT335283:NEUT_1146-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 24427; Mature: 24427

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFEQLRGIVAPLDRANIDTDAIIPKQFLKSIKRSGFGQNLFDEWRYLDYGEPGKDAAS
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHH
RQLNPDFILNQSRYQGAKILVTRDNFGCGSSREHAPWALQDYGFAVIIAPSFADIFYNNC
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEEHHCCEEEEECCCHHHHHHHCC
FKIGLLPIVLESPVVDRLIRDALTVDGYQLEINLEAQTVMALSGETYWFEVDHFRKHCLL
CEECHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCEEEEEHHHHHHHHHH
NGLDEIGLTLQHADRIRKFEINRRNEQPWLFL
CCHHHHCEEHHHHHHHHEEECCCCCCCCEEEC
>Mature Secondary Structure
MKKFEQLRGIVAPLDRANIDTDAIIPKQFLKSIKRSGFGQNLFDEWRYLDYGEPGKDAAS
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHH
RQLNPDFILNQSRYQGAKILVTRDNFGCGSSREHAPWALQDYGFAVIIAPSFADIFYNNC
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEEHHCCEEEEECCCHHHHHHHCC
FKIGLLPIVLESPVVDRLIRDALTVDGYQLEINLEAQTVMALSGETYWFEVDHFRKHCLL
CEECHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCEEEEEHHHHHHHHHH
NGLDEIGLTLQHADRIRKFEINRRNEQPWLFL
CCHHHHCEEHHHHHHHHEEECCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA