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The map label for this gene is proB

Identifier: 114330961

GI number: 114330961

Start: 988202

End: 989323

Strand: Direct

Name: proB

Synonym: Neut_0958

Alternate gene names: 114330961

Gene position: 988202-989323 (Clockwise)

Preceding gene: 114330960

Following gene: 114330962

Centisome position: 37.14

GC content: 52.32

Gene sequence:

>1122_bases
ATGACACGATCAATTCTGCAATCTGCACGCCGGATTATTGTAAAGGTTGGCAGCAGTCTGGTGACAAACGAAGGAAGCGG
CCTTGATCGTCAGGTGCTTGGAGGCTGGGCTGGACAAATTTCCCGACTCAAAAAAATGGGCAAGGAAGTCGTGCTGGTTT
CCTCCGGAGCGGTTGCTGAAGGTATGCAGCGCCTGGGCTGGAAAACGCGCCCCGCCGCGCTCTACGAGCTGCAGGCGGCA
GCGGCAGTCGGTCAGATGGACCTTGCCCAGGCCTATGCAGATTGTTTCAGTGAATATAAATTGCTGACTGCCCAGATTTT
GCTGACTCATGATGATTTATCGAGTCGTAAACGCTATCTGAATGCCCGTTCGACGATTCTCACATTGTTGAACCTGGATA
TTGTTCCAATTATCAACGAGAACGATACGGTGGTTTCTGATGAAATCCGGTTCGGTGACAATGATAATCTGGCGGCACTG
GTTGCCAATTTGATTGAAGCTGAGGTGCTGGTTATCCTGACTGATCAGGAGGGTCTGTTTACGGGGGATCCACGTAAGCA
TGCCGATGCAACACTGATTTCCGAGATCAGTGTCAGTGATCCGCATATCGAGACCATGGCTGGCGGAACGAGCAGTGGTA
TCGGTCGTGGTGGCATGCAGAGTAAAGTGATGGCGGCCAGACGGGCTGCTCGCAGTGGGGCTCATACAGTGATTGCATCA
GGCAGGGTGGAAAATGTGCTGGTTCGCCTGGCGAATGGAGAGGCAATTGGAACCAGTCTGCTGGCCGATATGCCGGTGAA
GGTTGCGCGTAAGTTATGGTTGGCCGATCATCTTCAGGTGCGGGGTAGCGTAGTATTAGATGATGGCGCCAGCAGAGCAT
TGCTGTCTGGAGGCAAAAGCCTGCTGCCTATCGGTGTGGTGGAGGTGAGAGGAAATTTTGAACGAGGTGAGGCCATATCT
TGCCTGAATACCAGTGGCCGCGAAATTGCCCGTGGCCTGGCCAATTACAGCGCACGTGAATCACACAAGATCATGCGTCG
CCCCTCCAGCCAGATCGAAGCTGTACTGGGTTATGTAGGTGAATATGAACTTGTTCACAGAGATAATCTGGTTATTCTGT
AA

Upstream 100 bases:

>100_bases
CCATTTCTGCTCTAACTGGGAGAGGGTGCCAGGCGCTGACCTATGCCATTATGGGATATCTGGAGCAACTACAGCCTGTT
ACCGAAGAAACTTGATTTTT

Downstream 100 bases:

>100_bases
TGGCTTGATCTGCTGGATTTACCTGTTTAGAAGGTTTAATACTATTTATAATGACTACCCATTTCTCTTTGCTCGGATCC
CGGTTACTTCAGTCTTATCA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTRSILQSARRIIVKVGSSLVTNEGSGLDRQVLGGWAGQISRLKKMGKEVVLVSSGAVAEGMQRLGWKTRPAALYELQAA
AAVGQMDLAQAYADCFSEYKLLTAQILLTHDDLSSRKRYLNARSTILTLLNLDIVPIINENDTVVSDEIRFGDNDNLAAL
VANLIEAEVLVILTDQEGLFTGDPRKHADATLISEISVSDPHIETMAGGTSSGIGRGGMQSKVMAARRAARSGAHTVIAS
GRVENVLVRLANGEAIGTSLLADMPVKVARKLWLADHLQVRGSVVLDDGASRALLSGGKSLLPIGVVEVRGNFERGEAIS
CLNTSGREIARGLANYSARESHKIMRRPSSQIEAVLGYVGEYELVHRDNLVIL

Sequences:

>Translated_373_residues
MTRSILQSARRIIVKVGSSLVTNEGSGLDRQVLGGWAGQISRLKKMGKEVVLVSSGAVAEGMQRLGWKTRPAALYELQAA
AAVGQMDLAQAYADCFSEYKLLTAQILLTHDDLSSRKRYLNARSTILTLLNLDIVPIINENDTVVSDEIRFGDNDNLAAL
VANLIEAEVLVILTDQEGLFTGDPRKHADATLISEISVSDPHIETMAGGTSSGIGRGGMQSKVMAARRAARSGAHTVIAS
GRVENVLVRLANGEAIGTSLLADMPVKVARKLWLADHLQVRGSVVLDDGASRALLSGGKSLLPIGVVEVRGNFERGEAIS
CLNTSGREIARGLANYSARESHKIMRRPSSQIEAVLGYVGEYELVHRDNLVIL
>Mature_372_residues
TRSILQSARRIIVKVGSSLVTNEGSGLDRQVLGGWAGQISRLKKMGKEVVLVSSGAVAEGMQRLGWKTRPAALYELQAAA
AVGQMDLAQAYADCFSEYKLLTAQILLTHDDLSSRKRYLNARSTILTLLNLDIVPIINENDTVVSDEIRFGDNDNLAALV
ANLIEAEVLVILTDQEGLFTGDPRKHADATLISEISVSDPHIETMAGGTSSGIGRGGMQSKVMAARRAARSGAHTVIASG
RVENVLVRLANGEAIGTSLLADMPVKVARKLWLADHLQVRGSVVLDDGASRALLSGGKSLLPIGVVEVRGNFERGEAISC
LNTSGREIARGLANYSARESHKIMRRPSSQIEAVLGYVGEYELVHRDNLVIL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=289, Percent_Identity=35.6401384083045, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI21361368, Length=291, Percent_Identity=35.3951890034364, Blast_Score=138, Evalue=7e-33,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=40.1639344262295, Blast_Score=260, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17569969, Length=273, Percent_Identity=35.1648351648352, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17569967, Length=273, Percent_Identity=35.1648351648352, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=33.3333333333333, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6321822, Length=419, Percent_Identity=34.3675417661098, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI21357643, Length=288, Percent_Identity=33.6805555555556, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_NITEC (Q0AHG4)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747183.1
- ProteinModelPortal:   Q0AHG4
- SMR:   Q0AHG4
- STRING:   Q0AHG4
- GeneID:   4272429
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0958
- NMPDR:   fig|335283.3.peg.1546
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q0AHG4
- ProtClustDB:   PRK05429
- BioCyc:   NEUT335283:NEUT_0958-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40016; Mature: 39885

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSILQSARRIIVKVGSSLVTNEGSGLDRQVLGGWAGQISRLKKMGKEVVLVSSGAVAE
CCHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHH
GMQRLGWKTRPAALYELQAAAAVGQMDLAQAYADCFSEYKLLTAQILLTHDDLSSRKRYL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHH
NARSTILTLLNLDIVPIINENDTVVSDEIRFGDNDNLAALVANLIEAEVLVILTDQEGLF
HHHHHHHHHHCCCEEEEECCCCCEEECCEEECCCCCHHHHHHHHHCCEEEEEEECCCCCC
TGDPRKHADATLISEISVSDPHIETMAGGTSSGIGRGGMQSKVMAARRAARSGAHTVIAS
CCCCCCCCCHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
GRVENVLVRLANGEAIGTSLLADMPVKVARKLWLADHLQVRGSVVLDDGASRALLSGGKS
CCHHEEHEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCC
LLPIGVVEVRGNFERGEAISCLNTSGREIARGLANYSARESHKIMRRPSSQIEAVLGYVG
CCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCC
EYELVHRDNLVIL
CEEEEECCCEEEC
>Mature Secondary Structure 
TRSILQSARRIIVKVGSSLVTNEGSGLDRQVLGGWAGQISRLKKMGKEVVLVSSGAVAE
CHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHH
GMQRLGWKTRPAALYELQAAAAVGQMDLAQAYADCFSEYKLLTAQILLTHDDLSSRKRYL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHH
NARSTILTLLNLDIVPIINENDTVVSDEIRFGDNDNLAALVANLIEAEVLVILTDQEGLF
HHHHHHHHHHCCCEEEEECCCCCEEECCEEECCCCCHHHHHHHHHCCEEEEEEECCCCCC
TGDPRKHADATLISEISVSDPHIETMAGGTSSGIGRGGMQSKVMAARRAARSGAHTVIAS
CCCCCCCCCHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
GRVENVLVRLANGEAIGTSLLADMPVKVARKLWLADHLQVRGSVVLDDGASRALLSGGKS
CCHHEEHEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCC
LLPIGVVEVRGNFERGEAISCLNTSGREIARGLANYSARESHKIMRRPSSQIEAVLGYVG
CCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCC
EYELVHRDNLVIL
CEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA