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The map label for this gene is def [H]

Identifier: 114330947

GI number: 114330947

Start: 975404

End: 975934

Strand: Reverse

Name: def [H]

Synonym: Neut_0944

Alternate gene names: 114330947

Gene position: 975934-975404 (Counterclockwise)

Preceding gene: 114330948

Following gene: 114330945

Centisome position: 36.67

GC content: 51.22

Gene sequence:

>531_bases
ATGATTAAACCCGTATTAAAAATGGGGGACCCACGCCTGTTGCAGCCTGCGCAGAGAATTGAGCAATTTGACACACCTGT
ACTCAAAGAATTATTGCAGGACATGCAAGATACGATGGCTGCACTCAATGGCGCCGGGCTCGCTGCCCCGCAAATCGGCA
TAAGCTTGCAAGTAGTCATCTTCGGGATAGAACACTCTCCGCGCTATCCGGATGTTGAAAGTGTGCCATTTACCGTGCTG
ATCAACCCCATACTCACCCCATTGACCGAAGAACAGGAAGAAGACTGGGAGGGTTGTCTGAGTATTCCGGGCATGCGCGG
ACTGGTTCCCCGCTACACTCACTTACGCTATCAGGGGGTGGATGAAACCGCTACTCCCATCGACAGAACAGTCGCCGGGT
TTCATGCGCGCGTGGTACAGCATGAATGTGACCACCTGAATGGGATACTCTATCCTATGCGAATCAATGATCTGCGAAAA
TTCGGTTATACCGATACACTATTCCCAGGCCAGACAATCGCAGAAGACTGA

Upstream 100 bases:

>100_bases
GAAAGCAAACAAGCTATCCCGCTGCAAGCTGCGCACAAGGCACTGGAAGATGCGATGATGAATGTCAGAAATATATTGGA
GCATCTGGTAAGGAATAATG

Downstream 100 bases:

>100_bases
TTCATTCTTTGCACTACCTTGTTAATAAGGTTTGACCACAACCAAGATAACAATCGCAAACAGCGCCAGTACCGGCAATT
CATTGAACCAGCGGTAGTAA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MIKPVLKMGDPRLLQPAQRIEQFDTPVLKELLQDMQDTMAALNGAGLAAPQIGISLQVVIFGIEHSPRYPDVESVPFTVL
INPILTPLTEEQEEDWEGCLSIPGMRGLVPRYTHLRYQGVDETATPIDRTVAGFHARVVQHECDHLNGILYPMRINDLRK
FGYTDTLFPGQTIAED

Sequences:

>Translated_176_residues
MIKPVLKMGDPRLLQPAQRIEQFDTPVLKELLQDMQDTMAALNGAGLAAPQIGISLQVVIFGIEHSPRYPDVESVPFTVL
INPILTPLTEEQEEDWEGCLSIPGMRGLVPRYTHLRYQGVDETATPIDRTVAGFHARVVQHECDHLNGILYPMRINDLRK
FGYTDTLFPGQTIAED
>Mature_176_residues
MIKPVLKMGDPRLLQPAQRIEQFDTPVLKELLQDMQDTMAALNGAGLAAPQIGISLQVVIFGIEHSPRYPDVESVPFTVL
INPILTPLTEEQEEDWEGCLSIPGMRGLVPRYTHLRYQGVDETATPIDRTVAGFHARVVQHECDHLNGILYPMRINDLRK
FGYTDTLFPGQTIAED

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=30.8571428571429, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI1789682, Length=147, Percent_Identity=34.0136054421769, Blast_Score=77, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24645728, Length=167, Percent_Identity=31.1377245508982, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=32.0512820512821, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19735; Mature: 19735

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPVLKMGDPRLLQPAQRIEQFDTPVLKELLQDMQDTMAALNGAGLAAPQIGISLQVVI
CCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
FGIEHSPRYPDVESVPFTVLINPILTPLTEEQEEDWEGCLSIPGMRGLVPRYTHLRYQGV
EEECCCCCCCCCCCCCCEEEHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHEECCC
DETATPIDRTVAGFHARVVQHECDHLNGILYPMRINDLRKFGYTDTLFPGQTIAED
CCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKPVLKMGDPRLLQPAQRIEQFDTPVLKELLQDMQDTMAALNGAGLAAPQIGISLQVVI
CCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
FGIEHSPRYPDVESVPFTVLINPILTPLTEEQEEDWEGCLSIPGMRGLVPRYTHLRYQGV
EEECCCCCCCCCCCCCCEEEHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHEECCC
DETATPIDRTVAGFHARVVQHECDHLNGILYPMRINDLRKFGYTDTLFPGQTIAED
CCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]