BacMap

Definition	Nitrosomonas eutropha C91, complete genome.
Accession	NC_008344
Length	2,661,057

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The map label for this gene is linC [H]

Identifier: 114330885

GI number: 114330885

Start: 901591

End: 902337

Strand: Reverse

Name: linC [H]

Synonym: Neut_0881

Alternate gene names: 114330885

Gene position: 902337-901591 (Counterclockwise)

Preceding gene: 114330886

Following gene: 114330884

Centisome position: 33.91

GC content: 49.53

Gene sequence:

>747_bases
ATGAATAATCTAGAAAATCAAGTCATTATCGTAACTGGCGGTGCCGCTGGGATAGGTGCTGCAATCACCACTGTATTAAC
GCAGCGAGGCGCATCTGTTGTCGTCGTTGATATCAATGAAGCAGCAGGCACAGCAATCGCGCAGAGCAATCCAGGTCAGA
TTGTTTTTTTAAAGGGTGATGTCTCCAGTGAGTCCGTTGCCAAGCAAGCCGTTGAACTGGCAATAGCCAGATTTGGCAAG
CTGACAGGGCTGGTCAATAATGCCCATGCGTCCACCCAAAAGCCACTAATAGAGCTGAGCAAAGAAGATTGGGCTTTATC
CTTTGGCACCGGCTTTAATGCAACGTTATACTTTATGAAAGCCGCTTATCCGGAGCTGACAAAATCTGGCGGCTCCATCG
TCAACTTTGGCTCAGGAGCAGCGCTCCTTGGACTCGAGCAGCAAGCCAGTTATACAGCAGCCAAAGAAGCTATCCGCGGA
TTAAGCAGGGTCGCCGCCAACGAATGGGCTAAAGACAACATTCGTGTCAATGTAGTCTCACCAAAGGCCTTGACCGAAGG
GGTCGCTCACTGGAAAGCTGAGTACCCGGAGCAATACGCAGCATCTGCCAGCAATATTCCGTTGCAGCGTTTTGGCGATC
CGCAAAAAGATGTTGCTCCAGTTGTTGCCTTTTTACTCAGCGAAGATAGTCGCTATATGACTGGACAAACACTAATGGCG
GACGGAGGCTCAATTCAGCTTAGGTAA

Upstream 100 bases:

>100_bases
ATAGGTCTATCAGGATAAATCTGTTCCCAGTCCCAGGCGACAGTAATTACTTGCTCAAACGACAGTGCAAAGTACAGCGA
CCTATAAAAGGGAAACAGAA

Downstream 100 bases:

>100_bases
ATGTAAATGTTCTGTTTGTTTTCAGCTCGACCGACCAAACCTGACTCCGCTATCAATAATTATTATATGGAGATATTGTT
ATGCACACTAATGGAAAAGC

Product: short-chain dehydrogenase/reductase SDR

Products: @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate!; NADH. [C]

Alternate protein names: 2,5-DDOL dehydrogenase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MNNLENQVIIVTGGAAGIGAAITTVLTQRGASVVVVDINEAAGTAIAQSNPGQIVFLKGDVSSESVAKQAVELAIARFGK
LTGLVNNAHASTQKPLIELSKEDWALSFGTGFNATLYFMKAAYPELTKSGGSIVNFGSGAALLGLEQQASYTAAKEAIRG
LSRVAANEWAKDNIRVNVVSPKALTEGVAHWKAEYPEQYAASASNIPLQRFGDPQKDVAPVVAFLLSEDSRYMTGQTLMA
DGGSIQLR

Sequences:

>Translated_248_residues
MNNLENQVIIVTGGAAGIGAAITTVLTQRGASVVVVDINEAAGTAIAQSNPGQIVFLKGDVSSESVAKQAVELAIARFGK
LTGLVNNAHASTQKPLIELSKEDWALSFGTGFNATLYFMKAAYPELTKSGGSIVNFGSGAALLGLEQQASYTAAKEAIRG
LSRVAANEWAKDNIRVNVVSPKALTEGVAHWKAEYPEQYAASASNIPLQRFGDPQKDVAPVVAFLLSEDSRYMTGQTLMA
DGGSIQLR
>Mature_248_residues
MNNLENQVIIVTGGAAGIGAAITTVLTQRGASVVVVDINEAAGTAIAQSNPGQIVFLKGDVSSESVAKQAVELAIARFGK
LTGLVNNAHASTQKPLIELSKEDWALSFGTGFNATLYFMKAAYPELTKSGGSIVNFGSGAALLGLEQQASYTAAKEAIRG
LSRVAANEWAKDNIRVNVVSPKALTEGVAHWKAEYPEQYAASASNIPLQRFGDPQKDVAPVVAFLLSEDSRYMTGQTLMA
DGGSIQLR

Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=254, Percent_Identity=31.8897637795276, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI40254992, Length=239, Percent_Identity=33.4728033472803, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI15277342, Length=256, Percent_Identity=31.640625, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI32483357, Length=252, Percent_Identity=30.5555555555556, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI59889578, Length=221, Percent_Identity=33.0316742081448, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI126723750, Length=250, Percent_Identity=29.6, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI7705925, Length=244, Percent_Identity=29.0983606557377, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI10190704, Length=251, Percent_Identity=28.6852589641434, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4758504, Length=248, Percent_Identity=28.2258064516129, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI31542939, Length=190, Percent_Identity=28.4210526315789, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4504505, Length=190, Percent_Identity=28.9473684210526, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI224922801, Length=173, Percent_Identity=29.4797687861272, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI66933014, Length=252, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI83715985, Length=250, Percent_Identity=27.6, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI5031737, Length=259, Percent_Identity=28.1853281853282, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789208, Length=246, Percent_Identity=32.1138211382114, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787335, Length=246, Percent_Identity=32.9268292682927, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788459, Length=245, Percent_Identity=31.0204081632653, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87082160, Length=246, Percent_Identity=26.0162601626016, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787905, Length=248, Percent_Identity=32.258064516129, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=28.740157480315, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790717, Length=241, Percent_Identity=28.6307053941909, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789378, Length=247, Percent_Identity=28.7449392712551, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787526, Length=254, Percent_Identity=27.5590551181102, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367175, Length=253, Percent_Identity=33.596837944664, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786812, Length=249, Percent_Identity=27.3092369477912, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788891, Length=259, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=263, Percent_Identity=30.4182509505703, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17561402, Length=254, Percent_Identity=33.0708661417323, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI115534694, Length=246, Percent_Identity=28.8617886178862, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17555706, Length=249, Percent_Identity=32.5301204819277, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17544670, Length=263, Percent_Identity=28.1368821292776, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI72000259, Length=261, Percent_Identity=28.3524904214559, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25147288, Length=250, Percent_Identity=30.8, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17565030, Length=263, Percent_Identity=30.4182509505703, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17560676, Length=247, Percent_Identity=28.7449392712551, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17538486, Length=267, Percent_Identity=28.4644194756554, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562910, Length=271, Percent_Identity=28.4132841328413, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=246, Percent_Identity=26.8292682926829, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17559104, Length=269, Percent_Identity=29.7397769516729, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17562906, Length=265, Percent_Identity=28.6792452830189, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI193204405, Length=263, Percent_Identity=27.3764258555133, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17563726, Length=263, Percent_Identity=27.3764258555133, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=266, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560150, Length=269, Percent_Identity=28.2527881040892, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=261, Percent_Identity=27.2030651340996, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=247, Percent_Identity=24.6963562753036, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17538182, Length=249, Percent_Identity=27.710843373494, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17562990, Length=268, Percent_Identity=27.9850746268657, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17568967, Length=198, Percent_Identity=32.8282828282828, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=264, Percent_Identity=26.1363636363636, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=274, Percent_Identity=27.007299270073, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=263, Percent_Identity=26.9961977186312, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=261, Percent_Identity=27.9693486590038, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324126, Length=254, Percent_Identity=27.5590551181102, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322226, Length=167, Percent_Identity=28.7425149700599, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI23397609, Length=250, Percent_Identity=32.8, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24643142, Length=243, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=32.258064516129, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28571526, Length=251, Percent_Identity=29.8804780876494, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24644339, Length=253, Percent_Identity=29.2490118577075, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24639444, Length=245, Percent_Identity=30.2040816326531, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI18859837, Length=238, Percent_Identity=30.2521008403361, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357041, Length=251, Percent_Identity=28.2868525896414, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17737361, Length=253, Percent_Identity=29.2490118577075, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.125 [C]

Molecular weight: Translated: 26029; Mature: 26029

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLENQVIIVTGGAAGIGAAITTVLTQRGASVVVVDINEAAGTAIAQSNPGQIVFLKGD
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCEEEEEECC
VSSESVAKQAVELAIARFGKLTGLVNNAHASTQKPLIELSKEDWALSFGTGFNATLYFMK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCCEEEEECCCCCCEEEEEH
AAYPELTKSGGSIVNFGSGAALLGLEQQASYTAAKEAIRGLSRVAANEWAKDNIRVNVVS
HHCHHHHHCCCCEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PKALTEGVAHWKAEYPEQYAASASNIPLQRFGDPQKDVAPVVAFLLSEDSRYMTGQTLMA
CHHHHHHHHHHHHCCCHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCEECCCEEEE
DGGSIQLR
CCCEEEEC
>Mature Secondary Structure
MNNLENQVIIVTGGAAGIGAAITTVLTQRGASVVVVDINEAAGTAIAQSNPGQIVFLKGD
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCEEEEEECC
VSSESVAKQAVELAIARFGKLTGLVNNAHASTQKPLIELSKEDWALSFGTGFNATLYFMK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCCEEEEECCCCCCEEEEEH
AAYPELTKSGGSIVNFGSGAALLGLEQQASYTAAKEAIRGLSRVAANEWAKDNIRVNVVS
HHCHHHHHCCCCEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PKALTEGVAHWKAEYPEQYAASASNIPLQRFGDPQKDVAPVVAFLLSEDSRYMTGQTLMA
CHHHHHHHHHHHHCCCHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCEECCCEEEE
DGGSIQLR
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 15.4 {2-deoxygluconate}} [C]

Substrates: @DEOX03.txt*2-Deoxy-D-Gluconate!; NAD(+) [C]

Specific reaction: @DEOX03.txt*2-Deoxy-D-Gluconate! + NAD(+) = @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate! + NADH. [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7515041; 12450824 [H]