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The map label for this gene is sucB [H]

Identifier: 114330865

GI number: 114330865

Start: 874659

End: 875951

Strand: Reverse

Name: sucB [H]

Synonym: Neut_0859

Alternate gene names: 114330865

Gene position: 875951-874659 (Counterclockwise)

Preceding gene: 114330866

Following gene: 114330856

Centisome position: 32.92

GC content: 49.19

Gene sequence:

>1293_bases
ATGCTGATCGAAGTCAAAGTCCCCGTATTTTCAGAATCAGTGACAGAAGGTACGCTGATCAATTGGCTTAAAAAACAGGG
AGAACATATTGAACGCGGTGAAAATCTGATTGATATTGAAACAGATAAGGTGGTTCTGGAGCTCCCTGCCCCGCAATCCG
GTGTTCTGGTTGAAATCGTCAAAAATGATGGGGCTATCGTCACCAGCGGGGAAATCATCGCTCGTATCGACACTGCAGCA
AAAGGCTTGAAAACAGATCCACAGCAACCTGCACAAACCGCTCAGGCTGAATCTGTTGACGATCCCAAACAGCCAGCAGC
TGATAAGACCGACGACGTATCACCACAACCAGGCCGCCCGCTTATGCCGGCTGCAAAAAGAGTCGCAGACGAGAATGGTC
TGACTGCCAAGGAAATAACAGCCATACATGGCACCGGCCGTGGTGAACGAATTACCAAGGAAGATGTATTAAACCACATC
AAGAATCGAGAAAGCTCTGCCACTAGCCAACAACCAGATTCCTCATCGAATCAGCCTGTCAGAAAAATTCAGCCTGACAC
AGCCTCATCAGCCTCTCAAACAGAACAATCCGCAAGACAAGAAAAACGTGTGCCCATGACCCGTCTACGCATGCGTATTG
CAGAACGGCTGGTACAGTCCCAATCCACCGCAGCTATTCTGACGACATTCAATGAAGTCAACATGCAGGCCATTCTGGAT
TTACGTGCCCGTTACAGGGTCAGTTTTGAGAAAGAACACGGCATCAAACTCGGGTTTACCTCATTCTTCATTAAGGCAGC
TATCGCAGCACTTAAAAAATTCCCCATCATCAACGCATCGGTGGATGGAAACGACATCATCTATCACGAATATTACGACA
TCGGCATAGCCGTAGCCAGCCCGCGCGGCCTGGTTGTGCCCATTATTCGCGATGCAGACAAACTTACCTTTGCTGAAATT
GAAAGACAGATTGCTGATCTGGCTAAACGAGCGCAGGATGGAAAACTGACCATAGAAGAGCTAACGGGAGGCACCTTTTC
TATTACCAACGGCGGTGTCTTCGGATCAATGCTCTCCACGCCGATCATCAATCCGCCACAAAGTGCTATTCTTGGTATCC
ATGCCACTAAAGAGCGCCCGGTAGTTGAAAATGGTCAGATCGTCATTCGGCCCATGAACTACCTCGCGCTTTCCTATGAT
CACCGCATTATCGATGGGCGTGAAGCCGTGCTATCACTGGTAGCCATGAAGGAAGCACTGGAATACCCGGCCAGCCCGCT
GTTCGATGATTGA

Upstream 100 bases:

>100_bases
CCCCTCGGTCGGGTATTTCGCAATGGATAGATTCAGGCAAAGAGAATTAATTGAAGCGGCATTTCGCGACACAATCTGAC
AAGGTTATGGAGAAATCATC

Downstream 100 bases:

>100_bases
GGAAACAATGTTATTCGTCTTGGGCGGACTGACGTAATCAGGAACAGCCATACAATCAATCAGCTGATTTTTTTGCTGTT
TCCCACTGTTCGGGCGTGTA

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MLIEVKVPVFSESVTEGTLINWLKKQGEHIERGENLIDIETDKVVLELPAPQSGVLVEIVKNDGAIVTSGEIIARIDTAA
KGLKTDPQQPAQTAQAESVDDPKQPAADKTDDVSPQPGRPLMPAAKRVADENGLTAKEITAIHGTGRGERITKEDVLNHI
KNRESSATSQQPDSSSNQPVRKIQPDTASSASQTEQSARQEKRVPMTRLRMRIAERLVQSQSTAAILTTFNEVNMQAILD
LRARYRVSFEKEHGIKLGFTSFFIKAAIAALKKFPIINASVDGNDIIYHEYYDIGIAVASPRGLVVPIIRDADKLTFAEI
ERQIADLAKRAQDGKLTIEELTGGTFSITNGGVFGSMLSTPIINPPQSAILGIHATKERPVVENGQIVIRPMNYLALSYD
HRIIDGREAVLSLVAMKEALEYPASPLFDD

Sequences:

>Translated_430_residues
MLIEVKVPVFSESVTEGTLINWLKKQGEHIERGENLIDIETDKVVLELPAPQSGVLVEIVKNDGAIVTSGEIIARIDTAA
KGLKTDPQQPAQTAQAESVDDPKQPAADKTDDVSPQPGRPLMPAAKRVADENGLTAKEITAIHGTGRGERITKEDVLNHI
KNRESSATSQQPDSSSNQPVRKIQPDTASSASQTEQSARQEKRVPMTRLRMRIAERLVQSQSTAAILTTFNEVNMQAILD
LRARYRVSFEKEHGIKLGFTSFFIKAAIAALKKFPIINASVDGNDIIYHEYYDIGIAVASPRGLVVPIIRDADKLTFAEI
ERQIADLAKRAQDGKLTIEELTGGTFSITNGGVFGSMLSTPIINPPQSAILGIHATKERPVVENGQIVIRPMNYLALSYD
HRIIDGREAVLSLVAMKEALEYPASPLFDD
>Mature_430_residues
MLIEVKVPVFSESVTEGTLINWLKKQGEHIERGENLIDIETDKVVLELPAPQSGVLVEIVKNDGAIVTSGEIIARIDTAA
KGLKTDPQQPAQTAQAESVDDPKQPAADKTDDVSPQPGRPLMPAAKRVADENGLTAKEITAIHGTGRGERITKEDVLNHI
KNRESSATSQQPDSSSNQPVRKIQPDTASSASQTEQSARQEKRVPMTRLRMRIAERLVQSQSTAAILTTFNEVNMQAILD
LRARYRVSFEKEHGIKLGFTSFFIKAAIAALKKFPIINASVDGNDIIYHEYYDIGIAVASPRGLVVPIIRDADKLTFAEI
ERQIADLAKRAQDGKLTIEELTGGTFSITNGGVFGSMLSTPIINPPQSAILGIHATKERPVVENGQIVIRPMNYLALSYD
HRIIDGREAVLSLVAMKEALEYPASPLFDD

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=233, Percent_Identity=52.7896995708155, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=29.9559471365639, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=30.0220750551876, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI110671329, Length=448, Percent_Identity=26.7857142857143, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI31711992, Length=460, Percent_Identity=29.1304347826087, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI260898739, Length=163, Percent_Identity=37.4233128834356, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786946, Length=427, Percent_Identity=48.0093676814988, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1786305, Length=413, Percent_Identity=29.0556900726392, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=424, Percent_Identity=39.622641509434, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17560088, Length=462, Percent_Identity=30.7359307359307, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=438, Percent_Identity=27.3972602739726, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=304, Percent_Identity=29.6052631578947, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=39.5784543325527, Blast_Score=309, Evalue=6e-85,
Organism=Saccharomyces cerevisiae, GI6324258, Length=461, Percent_Identity=28.4164859002169, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=55.3097345132743, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI18859875, Length=430, Percent_Identity=26.7441860465116, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24582497, Length=225, Percent_Identity=32.4444444444444, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20129315, Length=225, Percent_Identity=32.4444444444444, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 46984; Mature: 46984

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIEVKVPVFSESVTEGTLINWLKKQGEHIERGENLIDIETDKVVLELPAPQSGVLVEIV
CEEEEECCHHCCCCCCHHHHHHHHHCCCHHHCCCCEEEEECCEEEEECCCCCCCEEEEEE
KNDGAIVTSGEIIARIDTAAKGLKTDPQQPAQTAQAESVDDPKQPAADKTDDVSPQPGRP
ECCCEEEECCCEEEEEHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
LMPAAKRVADENGLTAKEITAIHGTGRGERITKEDVLNHIKNRESSATSQQPDSSSNQPV
CCHHHHHHHCCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCH
RKIQPDTASSASQTEQSARQEKRVPMTRLRMRIAERLVQSQSTAAILTTFNEVNMQAILD
HHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
LRARYRVSFEKEHGIKLGFTSFFIKAAIAALKKFPIINASVDGNDIIYHEYYDIGIAVAS
HHHHHEEEECCCCCEEECHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEEEEEEEEEEC
PRGLVVPIIRDADKLTFAEIERQIADLAKRAQDGKLTIEELTGGTFSITNGGVFGSMLST
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHHHHCC
PIINPPQSAILGIHATKERPVVENGQIVIRPMNYLALSYDHRIIDGREAVLSLVAMKEAL
CCCCCCHHHEEEEEECCCCCCCCCCCEEEEEHHHEEEECCCEEECCHHHHHHHHHHHHHH
EYPASPLFDD
HCCCCCCCCC
>Mature Secondary Structure
MLIEVKVPVFSESVTEGTLINWLKKQGEHIERGENLIDIETDKVVLELPAPQSGVLVEIV
CEEEEECCHHCCCCCCHHHHHHHHHCCCHHHCCCCEEEEECCEEEEECCCCCCCEEEEEE
KNDGAIVTSGEIIARIDTAAKGLKTDPQQPAQTAQAESVDDPKQPAADKTDDVSPQPGRP
ECCCEEEECCCEEEEEHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
LMPAAKRVADENGLTAKEITAIHGTGRGERITKEDVLNHIKNRESSATSQQPDSSSNQPV
CCHHHHHHHCCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCH
RKIQPDTASSASQTEQSARQEKRVPMTRLRMRIAERLVQSQSTAAILTTFNEVNMQAILD
HHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
LRARYRVSFEKEHGIKLGFTSFFIKAAIAALKKFPIINASVDGNDIIYHEYYDIGIAVAS
HHHHHEEEECCCCCEEECHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEEEEEEEEEEC
PRGLVVPIIRDADKLTFAEIERQIADLAKRAQDGKLTIEELTGGTFSITNGGVFGSMLST
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHHHHCC
PIINPPQSAILGIHATKERPVVENGQIVIRPMNYLALSYDHRIIDGREAVLSLVAMKEAL
CCCCCCHHHEEEEEECCCCCCCCCCCEEEEEHHHEEEECCCEEECCHHHHHHHHHHHHHH
EYPASPLFDD
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]