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The map label for this gene is nucI [H]

Identifier: 114330856

GI number: 114330856

Start: 868492

End: 869367

Strand: Reverse

Name: nucI [H]

Synonym: Neut_0850

Alternate gene names: 114330856

Gene position: 869367-868492 (Counterclockwise)

Preceding gene: 114330865

Following gene: 114330853

Centisome position: 32.67

GC content: 47.72

Gene sequence:

>876_bases
ATGCATTTTACCAGAGCCCCCCGGATACCACTCAACCCGTCACTCTCTTTCCTGAAATTTTGTCTCTTTACAATCTTTCT
GATTTTTCTGCATGCCCAACCGGGGCTGGCTGACACCATCTATCGCTCTATCAATCCCCATGGTCGAATCCTCTATTCTG
ATATCCCCACCCCAACTGCAAAACCTCTGCAACTTGCTGCTCAGCCAGTACGGAGCAAATATCGGGTCACTAGAGTTATC
GACGGCGATACCGTCGTGCTTGAGAATAACAAACGGGTAAGATTGCTGGGTGTGAATGCACCCGAGATCGAAAACCGGTT
TCATCCGGCAGAGCCAGGCGGGATTGAAGCAAAAAAATGGCTGCAAGGAAAACTACAAGGACGCAGTGTTTATCTGGAAC
ATGATCGGCAAACGCAAGATCACTATAAAAGAATGCTTGCTCATCTATATCTTGCAGATGGAGAACATATCAACCTGTCT
CTGGTGGAAAAAGGGCTAGCTGTTGTCACACTGATATCTCCAAACCTGCTGCATGCCAATACCCTGATCAAGGCACAACA
ACGGGCAGAAACAAAGAAACTGGGGATCTGGTCAATGAAGCAATACCAGCCACGTCAGCTGGTCAAACTAACCGAAAAAC
CATTTGGTTGGCAGCGCTACCGGGTAAAGGTCACGGCACTCAAACGCAATCGCCGATTCAGCCGCCTTATCATCGGCAAA
AATATCGACATCTCTATTGCCAACCAGGATCTGGCCTTATTCCCACGTCTGGAAACTTATCTGAACAAGCCGTTGGAGGT
ACGTGGCTGGGTATCACGCAAGAAAAACCATTTTTCAATACGTATTCAGCACCCGAGTGCATTGATCCTGCATTAA

Upstream 100 bases:

>100_bases
ATTTCAGTGACATGCGTAACATCTCCAAGGTTGCATCCTAATTCCAATACTGGCCGCATAGACATTCCAACCCGAACATT
CCCCTAACATAATCCAATTC

Downstream 100 bases:

>100_bases
GATCACTTCTGAAGATTGGCTTTCAGACGAGGTACCCTTAAGAAAAATCACTGTAGCGTGACCGTTTGCGCCATTGAATA
CGTGGAATAACTAGCCCCAA

Product: nuclease

Products: NA

Alternate protein names: TNase; Micrococcal nuclease; Staphylococcal nuclease [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MHFTRAPRIPLNPSLSFLKFCLFTIFLIFLHAQPGLADTIYRSINPHGRILYSDIPTPTAKPLQLAAQPVRSKYRVTRVI
DGDTVVLENNKRVRLLGVNAPEIENRFHPAEPGGIEAKKWLQGKLQGRSVYLEHDRQTQDHYKRMLAHLYLADGEHINLS
LVEKGLAVVTLISPNLLHANTLIKAQQRAETKKLGIWSMKQYQPRQLVKLTEKPFGWQRYRVKVTALKRNRRFSRLIIGK
NIDISIANQDLALFPRLETYLNKPLEVRGWVSRKKNHFSIRIQHPSALILH

Sequences:

>Translated_291_residues
MHFTRAPRIPLNPSLSFLKFCLFTIFLIFLHAQPGLADTIYRSINPHGRILYSDIPTPTAKPLQLAAQPVRSKYRVTRVI
DGDTVVLENNKRVRLLGVNAPEIENRFHPAEPGGIEAKKWLQGKLQGRSVYLEHDRQTQDHYKRMLAHLYLADGEHINLS
LVEKGLAVVTLISPNLLHANTLIKAQQRAETKKLGIWSMKQYQPRQLVKLTEKPFGWQRYRVKVTALKRNRRFSRLIIGK
NIDISIANQDLALFPRLETYLNKPLEVRGWVSRKKNHFSIRIQHPSALILH
>Mature_291_residues
MHFTRAPRIPLNPSLSFLKFCLFTIFLIFLHAQPGLADTIYRSINPHGRILYSDIPTPTAKPLQLAAQPVRSKYRVTRVI
DGDTVVLENNKRVRLLGVNAPEIENRFHPAEPGGIEAKKWLQGKLQGRSVYLEHDRQTQDHYKRMLAHLYLADGEHINLS
LVEKGLAVVTLISPNLLHANTLIKAQQRAETKKLGIWSMKQYQPRQLVKLTEKPFGWQRYRVKVTALKRNRRFSRLIIGK
NIDISIANQDLALFPRLETYLNKPLEVRGWVSRKKNHFSIRIQHPSALILH

Specific function: Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5'-position of the phosphodiester bond [H]

COG id: COG1525

COG function: function code L; Micrococcal nuclease (thermonuclease) homologs

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thermonuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016071
- InterPro:   IPR006021
- InterPro:   IPR002071 [H]

Pfam domain/function: PF00565 SNase [H]

EC number: =3.1.31.1 [H]

Molecular weight: Translated: 33602; Mature: 33602

Theoretical pI: Translated: 11.09; Mature: 11.09

Prosite motif: PS50830 TNASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFTRAPRIPLNPSLSFLKFCLFTIFLIFLHAQPGLADTIYRSINPHGRILYSDIPTPTA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCC
KPLQLAAQPVRSKYRVTRVIDGDTVVLENNKRVRLLGVNAPEIENRFHPAEPGGIEAKKW
CHHHHHHHHHHCCEEEEEEECCCEEEEECCCEEEEEECCCCHHHCCCCCCCCCCCCHHHH
LQGKLQGRSVYLEHDRQTQDHYKRMLAHLYLADGEHINLSLVEKGLAVVTLISPNLLHAN
HHHCCCCCEEEEECCCCHHHHHHHHHHHHEECCCCEEEEEEECCCEEEEEEECCCEEEHH
TLIKAQQRAETKKLGIWSMKQYQPRQLVKLTEKPFGWQRYRVKVTALKRNRRFSRLIIGK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEEECC
NIDISIANQDLALFPRLETYLNKPLEVRGWVSRKKNHFSIRIQHPSALILH
CCEEEEECCCEEECCCHHHHHCCCEEEEEHHCCCCCEEEEEEECCCEEEEC
>Mature Secondary Structure
MHFTRAPRIPLNPSLSFLKFCLFTIFLIFLHAQPGLADTIYRSINPHGRILYSDIPTPTA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCC
KPLQLAAQPVRSKYRVTRVIDGDTVVLENNKRVRLLGVNAPEIENRFHPAEPGGIEAKKW
CHHHHHHHHHHCCEEEEEEECCCEEEEECCCEEEEEECCCCHHHCCCCCCCCCCCCHHHH
LQGKLQGRSVYLEHDRQTQDHYKRMLAHLYLADGEHINLSLVEKGLAVVTLISPNLLHAN
HHHCCCCCEEEEECCCCHHHHHHHHHHHHEECCCCEEEEEEECCCEEEEEEECCCEEEHH
TLIKAQQRAETKKLGIWSMKQYQPRQLVKLTEKPFGWQRYRVKVTALKRNRRFSRLIIGK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEEECC
NIDISIANQDLALFPRLETYLNKPLEVRGWVSRKKNHFSIRIQHPSALILH
CCEEEEECCCEEECCCHHHHHCCCEEEEEHHCCCCCEEEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1408843 [H]