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The map label for this gene is ispB [H]

Identifier: 114330832

GI number: 114330832

Start: 841237

End: 842205

Strand: Reverse

Name: ispB [H]

Synonym: Neut_0825

Alternate gene names: 114330832

Gene position: 842205-841237 (Counterclockwise)

Preceding gene: 114330837

Following gene: 114330830

Centisome position: 31.65

GC content: 49.23

Gene sequence:

>969_bases
GTGTCAATCGAGTCAATCAGAAACGTTATCGCCAAAGATATGGGCGAGGTGGATAACGTTATCCAGCAAAAGCTTCATTC
TCAAGTTGTTCTTGTTCGACAGGTCAGCGAATACATCATCCATAGCGGCGGAAAAAGGTTACGGCCAGCATTAGTCATCC
TTGCCGCAGGGTTATACGATTACAAGGGTACGCATCACTATAATCTCGCTGCTGTTGTCGAATTTATCCATACCGCAACA
CTATTACACGATGACGTAGTGGATGAATCCCAACTGCGCCGCAATCAGGCCACAGCCAATGCTTTATTTGGCAACGCTGC
CAGTGTGTTGGTGGGTGATTTTCTGTACTCGCGTGCTTTCCAGATGATGGTGGAAGTCGATAACATGCGAGTTATGGAGG
TGCTGGCCGACGCAACCAACACCATTGCCGAGGGTGAGGTACTGCAATTACTTAACTGCCGTGACCCAGACGTTGAAGAG
GAAAATTACCTGCAGGTTATCCGTTTCAAAACCGCCAAGCTTTTTGAAGCTGCTGGCCGGCTGGGAGCGATACTGGGCGG
AGCCACTCCGGCTGAAGAAGAAGCACTGGCTGCTTATGGCATGCATCTGGGCACAGCATTCCAGCTGGTGGATGATTTGC
TGGATTATTCGGGTAATGATCATGACACTGGTAAAAATCTGGGTGACGATCTGGCAGAAGGAAAGCCGACTCTGCCACTG
ATCTTCGCCATGCGGGCTGGCACGACCGACCAAGCAAATACGGTCCGTCATGCTATTGAATCTGGTGGAAAAGACGGGTT
TGGACCAGTACTCGAAATCATCCAGAAAACTGGTGCGCTGGATTACGCAAAGCAATGCGCGCAGAAAGAAGCCAACATGG
CCACTTCCGTAATCTCTACGCTCCGTGATTCTATCTATAAACAATGTTTGCTAGAATTGGCGGAGTTCGCTGTTTCGAGA
AATTACTAA

Upstream 100 bases:

>100_bases
GATACGCCGCACAATATGTATCTCGGCTGTTTTGCCTGCAGGAATCCTTGACACCCGGGCAGGGACATTTCTACCATAGC
GGTTTCTTCAGGGAGTCATT

Downstream 100 bases:

>100_bases
TTTTTACCAGAATTGCCAGCTTCAGCAATTATCACTGGAAATCCTAAATATTTCCTTGCTAGTGCTGTTTTCGATAAACT
ATTTCATTCGGGGTGTAGCT

Product: farnesyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSIESIRNVIAKDMGEVDNVIQQKLHSQVVLVRQVSEYIIHSGGKRLRPALVILAAGLYDYKGTHHYNLAAVVEFIHTAT
LLHDDVVDESQLRRNQATANALFGNAASVLVGDFLYSRAFQMMVEVDNMRVMEVLADATNTIAEGEVLQLLNCRDPDVEE
ENYLQVIRFKTAKLFEAAGRLGAILGGATPAEEEALAAYGMHLGTAFQLVDDLLDYSGNDHDTGKNLGDDLAEGKPTLPL
IFAMRAGTTDQANTVRHAIESGGKDGFGPVLEIIQKTGALDYAKQCAQKEANMATSVISTLRDSIYKQCLLELAEFAVSR
NY

Sequences:

>Translated_322_residues
MSIESIRNVIAKDMGEVDNVIQQKLHSQVVLVRQVSEYIIHSGGKRLRPALVILAAGLYDYKGTHHYNLAAVVEFIHTAT
LLHDDVVDESQLRRNQATANALFGNAASVLVGDFLYSRAFQMMVEVDNMRVMEVLADATNTIAEGEVLQLLNCRDPDVEE
ENYLQVIRFKTAKLFEAAGRLGAILGGATPAEEEALAAYGMHLGTAFQLVDDLLDYSGNDHDTGKNLGDDLAEGKPTLPL
IFAMRAGTTDQANTVRHAIESGGKDGFGPVLEIIQKTGALDYAKQCAQKEANMATSVISTLRDSIYKQCLLELAEFAVSR
NY
>Mature_321_residues
SIESIRNVIAKDMGEVDNVIQQKLHSQVVLVRQVSEYIIHSGGKRLRPALVILAAGLYDYKGTHHYNLAAVVEFIHTATL
LHDDVVDESQLRRNQATANALFGNAASVLVGDFLYSRAFQMMVEVDNMRVMEVLADATNTIAEGEVLQLLNCRDPDVEEE
NYLQVIRFKTAKLFEAAGRLGAILGGATPAEEEALAAYGMHLGTAFQLVDDLLDYSGNDHDTGKNLGDDLAEGKPTLPLI
FAMRAGTTDQANTVRHAIESGGKDGFGPVLEIIQKTGALDYAKQCAQKEANMATSVISTLRDSIYKQCLLELAEFAVSRN
Y

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=320, Percent_Identity=29.0625, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI4758430, Length=217, Percent_Identity=24.8847926267281, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI83700220, Length=217, Percent_Identity=24.8847926267281, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=53.7267080745342, Blast_Score=356, Evalue=1e-100,
Organism=Escherichia coli, GI1786623, Length=219, Percent_Identity=34.2465753424658, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=240, Percent_Identity=31.6666666666667, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319475, Length=221, Percent_Identity=34.841628959276, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=281, Percent_Identity=25.6227758007117, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24651612, Length=294, Percent_Identity=28.9115646258503, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281365769, Length=294, Percent_Identity=24.8299319727891, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24660002, Length=294, Percent_Identity=24.8299319727891, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35190; Mature: 35059

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIESIRNVIAKDMGEVDNVIQQKLHSQVVLVRQVSEYIIHSGGKRLRPALVILAAGLYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YKGTHHYNLAAVVEFIHTATLLHDDVVDESQLRRNQATANALFGNAASVLVGDFLYSRAF
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QMMVEVDNMRVMEVLADATNTIAEGEVLQLLNCRDPDVEEENYLQVIRFKTAKLFEAAGR
HHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGAILGGATPAEEEALAAYGMHLGTAFQLVDDLLDYSGNDHDTGKNLGDDLAEGKPTLPL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHH
IFAMRAGTTDQANTVRHAIESGGKDGFGPVLEIIQKTGALDYAKQCAQKEANMATSVIST
EEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LRDSIYKQCLLELAEFAVSRNY
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIESIRNVIAKDMGEVDNVIQQKLHSQVVLVRQVSEYIIHSGGKRLRPALVILAAGLYD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YKGTHHYNLAAVVEFIHTATLLHDDVVDESQLRRNQATANALFGNAASVLVGDFLYSRAF
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QMMVEVDNMRVMEVLADATNTIAEGEVLQLLNCRDPDVEEENYLQVIRFKTAKLFEAAGR
HHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGAILGGATPAEEEALAAYGMHLGTAFQLVDDLLDYSGNDHDTGKNLGDDLAEGKPTLPL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHH
IFAMRAGTTDQANTVRHAIESGGKDGFGPVLEIIQKTGALDYAKQCAQKEANMATSVIST
EEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LRDSIYKQCLLELAEFAVSRNY
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]