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The map label for this gene is fadA [H]

Identifier: 114330740

GI number: 114330740

Start: 739000

End: 740202

Strand: Direct

Name: fadA [H]

Synonym: Neut_0725

Alternate gene names: 114330740

Gene position: 739000-740202 (Clockwise)

Preceding gene: 114330739

Following gene: 114330743

Centisome position: 27.77

GC content: 55.53

Gene sequence:

>1203_bases
ATGACCAGGCAAGTGCAGGAGGCTTATATCGTAGCCGCTACACGCACACCGGTAGGTAAAGCGCCACGGGGCATGTTCAA
GGATGCTCGGCCGGACGACATGTTAGTGCATGTGCTGCAGGTAGTGCTGAAACAGTGTGCAGGGCTGGATCCGGCAGCGG
TTGAAGATGTTATCGTCGGTTGTGCCATGCCGGAAGCGGAACAGGGGATTAATGTGGCGCGCGTAGCACTGCTGCTGGCT
GGTCTGCCGGTTAGCGTGCCGGGTGTAACAATTAATCGGTTTTGCGCTTCCGGCCTGCAGGCGGTTGCCATGGCAGCCGA
CCGGATTCGGTTAGGTGAAGCGGATGTCATCATTGCTGGCGGCACCGAAAGCATGAGTATGGTGCCGATGATGGGTAACA
AAGTGGCGATGAACCCGGCTTTATTTAAGCCAGAGAGTGAACAGGTAGCCATTGCTTATGGCATGGGGATTACTGCCGAA
AAGGTGGCGGAACAATGGAAGGTCAGCCGTGAGGCGCAGGATGAATTTGCACTGGAAAGCCATCACCGTGCGATTCGCGC
CATCGAACAAGGGGAATTCAGGGATGAAATTTCTCCTTATCCCGTTCATGAAGACCGGCCGGACCTGAATACGCACGAAA
TCCACGATTTCTCCGTTATCAGGGATACTGACGAGGGACCCCGGGCGAATAGCAGCCCGGAGGCACTCGCCCGGTTACGT
CCGGTGTTTGCAGCACGAGGATCTGTCACGGCGGGTAACAGCTCGCAAATGTCGGATGGAGCAGGTGCGGTGGTGGTGGC
GAGCGAGGCAGCTTTGCAGCGCTTTAATCTGACACCGATCGGGCGATTTATTGGTTATGCGGTGGCTGGTGTGCCGCCGG
AAATCATGGGGATAGGACCCGTCGAGGCGATTCCTAAAGTGCTGAAACAAACCGGCATCAAACAGGATGAACTTGACTGG
ATTGAGTTGAACGAGGCATTCGCTGCGCAAAGTCTGGCTGTTATCCATGATCTCGGATTGGATCGGGCAAAAGTTAATCC
ACTGGGTGGGGCCATTGCACTGGGTCATCCACTGGGTGCAACCGGTGCGATTCGCGTGGCAACATTACTGAGCGGATTAC
GTCGTCACAAACTCAAATATGGCATGGTCACCATGTGCATTGGCGGCGGAATGGGAGCGGCAGGCGTATTTGAAGCGCTA
TAA

Upstream 100 bases:

>100_bases
CATACGCTGAAAACCGGTAAACCGCTGCGCAACTGAGTGATTTGGAGTTGCGGAAGATGGGCGTGATGAAATAAAAATTC
AAGTATTGAAGGAGAAACTG

Downstream 100 bases:

>100_bases
ACAAAGCATCAGTCCGATAGAGCAAATCGGACTGATTTTGGCGGGCGGCTGATTTATTGGGCAGGTATCTGTAATCTTCC
TGTAAAAATTGTTTGATTAC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTRQVQEAYIVAATRTPVGKAPRGMFKDARPDDMLVHVLQVVLKQCAGLDPAAVEDVIVGCAMPEAEQGINVARVALLLA
GLPVSVPGVTINRFCASGLQAVAMAADRIRLGEADVIIAGGTESMSMVPMMGNKVAMNPALFKPESEQVAIAYGMGITAE
KVAEQWKVSREAQDEFALESHHRAIRAIEQGEFRDEISPYPVHEDRPDLNTHEIHDFSVIRDTDEGPRANSSPEALARLR
PVFAARGSVTAGNSSQMSDGAGAVVVASEAALQRFNLTPIGRFIGYAVAGVPPEIMGIGPVEAIPKVLKQTGIKQDELDW
IELNEAFAAQSLAVIHDLGLDRAKVNPLGGAIALGHPLGATGAIRVATLLSGLRRHKLKYGMVTMCIGGGMGAAGVFEAL

Sequences:

>Translated_400_residues
MTRQVQEAYIVAATRTPVGKAPRGMFKDARPDDMLVHVLQVVLKQCAGLDPAAVEDVIVGCAMPEAEQGINVARVALLLA
GLPVSVPGVTINRFCASGLQAVAMAADRIRLGEADVIIAGGTESMSMVPMMGNKVAMNPALFKPESEQVAIAYGMGITAE
KVAEQWKVSREAQDEFALESHHRAIRAIEQGEFRDEISPYPVHEDRPDLNTHEIHDFSVIRDTDEGPRANSSPEALARLR
PVFAARGSVTAGNSSQMSDGAGAVVVASEAALQRFNLTPIGRFIGYAVAGVPPEIMGIGPVEAIPKVLKQTGIKQDELDW
IELNEAFAAQSLAVIHDLGLDRAKVNPLGGAIALGHPLGATGAIRVATLLSGLRRHKLKYGMVTMCIGGGMGAAGVFEAL
>Mature_399_residues
TRQVQEAYIVAATRTPVGKAPRGMFKDARPDDMLVHVLQVVLKQCAGLDPAAVEDVIVGCAMPEAEQGINVARVALLLAG
LPVSVPGVTINRFCASGLQAVAMAADRIRLGEADVIIAGGTESMSMVPMMGNKVAMNPALFKPESEQVAIAYGMGITAEK
VAEQWKVSREAQDEFALESHHRAIRAIEQGEFRDEISPYPVHEDRPDLNTHEIHDFSVIRDTDEGPRANSSPEALARLRP
VFAARGSVTAGNSSQMSDGAGAVVVASEAALQRFNLTPIGRFIGYAVAGVPPEIMGIGPVEAIPKVLKQTGIKQDELDWI
ELNEAFAAQSLAVIHDLGLDRAKVNPLGGAIALGHPLGATGAIRVATLLSGLRRHKLKYGMVTMCIGGGMGAAGVFEAL

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=45.5696202531646, Blast_Score=298, Evalue=5e-81,
Organism=Homo sapiens, GI167614485, Length=401, Percent_Identity=39.4014962593516, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI148539872, Length=395, Percent_Identity=39.2405063291139, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI4557237, Length=406, Percent_Identity=35.2216748768473, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI194353979, Length=393, Percent_Identity=34.0966921119593, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI4504327, Length=442, Percent_Identity=30.5429864253394, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=43.3583959899749, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1787663, Length=412, Percent_Identity=42.2330097087379, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1788554, Length=406, Percent_Identity=42.1182266009852, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI87082165, Length=407, Percent_Identity=39.5577395577396, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1788683, Length=437, Percent_Identity=31.5789473684211, Blast_Score=163, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=42.643391521197, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI25147385, Length=402, Percent_Identity=32.3383084577114, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17535921, Length=404, Percent_Identity=32.6732673267327, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=435, Percent_Identity=31.0344827586207, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=400, Percent_Identity=27.75, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322031, Length=402, Percent_Identity=42.5373134328358, Blast_Score=297, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6325229, Length=408, Percent_Identity=35.5392156862745, Blast_Score=213, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24655093, Length=404, Percent_Identity=42.0792079207921, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI17648125, Length=410, Percent_Identity=40.4878048780488, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24640423, Length=408, Percent_Identity=34.0686274509804, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI17137578, Length=440, Percent_Identity=31.3636363636364, Blast_Score=168, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 42319; Mature: 42188

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQVQEAYIVAATRTPVGKAPRGMFKDARPDDMLVHVLQVVLKQCAGLDPAAVEDVIVG
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
CAMPEAEQGINVARVALLLAGLPVSVPGVTINRFCASGLQAVAMAADRIRLGEADVIIAG
CCCCCHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEC
GTESMSMVPMMGNKVAMNPALFKPESEQVAIAYGMGITAEKVAEQWKVSREAQDEFALES
CCCCCEEEECCCCEEECCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
HHRAIRAIEQGEFRDEISPYPVHEDRPDLNTHEIHDFSVIRDTDEGPRANSSPEALARLR
HHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHH
PVFAARGSVTAGNSSQMSDGAGAVVVASEAALQRFNLTPIGRFIGYAVAGVPPEIMGIGP
HHHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHHCCCCHHHHHHHHHHCCCCHHHCCCCC
VEAIPKVLKQTGIKQDELDWIELNEAFAAQSLAVIHDLGLDRAKVNPLGGAIALGHPLGA
HHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEECCCCCC
TGAIRVATLLSGLRRHKLKYGMVTMCIGGGMGAAGVFEAL
CHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
TRQVQEAYIVAATRTPVGKAPRGMFKDARPDDMLVHVLQVVLKQCAGLDPAAVEDVIVG
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
CAMPEAEQGINVARVALLLAGLPVSVPGVTINRFCASGLQAVAMAADRIRLGEADVIIAG
CCCCCHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEC
GTESMSMVPMMGNKVAMNPALFKPESEQVAIAYGMGITAEKVAEQWKVSREAQDEFALES
CCCCCEEEECCCCEEECCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
HHRAIRAIEQGEFRDEISPYPVHEDRPDLNTHEIHDFSVIRDTDEGPRANSSPEALARLR
HHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHH
PVFAARGSVTAGNSSQMSDGAGAVVVASEAALQRFNLTPIGRFIGYAVAGVPPEIMGIGP
HHHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHHCCCCHHHHHHHHHHCCCCHHHCCCCC
VEAIPKVLKQTGIKQDELDWIELNEAFAAQSLAVIHDLGLDRAKVNPLGGAIALGHPLGA
HHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEECCCCCC
TGAIRVATLLSGLRRHKLKYGMVTMCIGGGMGAAGVFEAL
CHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]