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The map label for this gene is pmbA [H]

Identifier: 114330703

GI number: 114330703

Start: 693065

End: 694429

Strand: Reverse

Name: pmbA [H]

Synonym: Neut_0688

Alternate gene names: 114330703

Gene position: 694429-693065 (Counterclockwise)

Preceding gene: 114330708

Following gene: 114330702

Centisome position: 26.1

GC content: 51.87

Gene sequence:

>1365_bases
ATGAATAATTCTAATTATCCGGAACCGCTGCAGCCATTCACCTACCCCTACGAGACCCTGCAGCAAATTGCAAATGATGT
GCTAACACTTGCCAAAAAAGGAGGTGCCGACGCATGCGAGATGCATGTTTCCGATGGCTCTGGCCAGAACGTCACCGTGC
GCCAGGGGGAAGTGGAGACTATTGAATATACACGCGACAAAGGGCTATCTGTCACCGTTCATATTGGACATAAGCGGGGC
AATGCTAGCAGCTCGGATTTTTCTCCACAGGCCATTCGCGAAACCATCTCTGCCGCTCTGTCGATTGCCCGATACACGGC
TGACGATATTTATGCGGGACTCGCTGACAAGGATCTGCTGGCGACTTCTTTCCCTGACCTTGATCTCTATCACCCGTGGT
CACTGTCTGTTGAAACAGCCATTGAGCTGGCACGCCGGTGTGAAGCCGCAGCACTGGCAACAGATAAACGTATCACCAAT
TCGGAAGGCGCAAGTGTATCGGCCGGTGCTTCCCGGTTTGTATATGCCAACAGTCATGGCTTCAATGCGGGTTATCCACT
ATCTCGCCATAGCATCAGCTGCGCGGTCATTGCCGGGGAACAAAATAACATGCAGCGCGATTACTGGTACAGCGTTGCGC
GTGCGGCCAGCGATCTCGAAGCCATTGAGGAAATAGGCCAAAAAGCCGGTATGCGCACCCTGGCACGCCTCGGTGCCAGA
AAAATTGCTACCTGCGAAGTGCCGGTGCTGTTTGAATCAACTATTTCTTCCGGTCTGCTGGGGTATTTTGTCAAGGCAAT
CAGTGGCAGCAGCCTTTATCGCAAATCCTCCTTTCTGCTGGACAGCATCGGCAAACAGGTATTTCCTGCAACCATTCAGA
TCAGTGAGTTGCCGCACCTGCCAAAAGGTCTGGCAAGCTGTGCGTTTGACGATGAAGGGGTGGCGACTCATCCGCGCAAG
GTGGTGGAAAACGGTATCGTGCAAGGGTATTTTCTGGGCAGCTACACAGCGCGTAAACTTGGTATGCGTTCCACTGGTAA
TGCCGGTGGCAATCATAATCTGATCCTGGAAAACGATGTATCACTGCCGTTCGATGCTCTACTGAAAAAAATGAATAAAG
GGTTACTGGTTACTGAATTGCTTGGCCATGGTGTCAACCTCGTCACTGGAGATTATTCCCAGGGCGCCGCCGGATTTTGG
GTGGAAAACGGTGAGATCAGCTACCCCGTTGAGGAGATCACTATTGCCGGAAATCTGAAGGAAATGTTGTCCGGTATTGT
TGCCGTTGGTGACGATATCATCGTGCGCGGCTCACGCCACTGTGGTTCGCTGCTGATTGAACGCATGATCATCGCAGGAC
ATTAA

Upstream 100 bases:

>100_bases
AACGATCATTCTTCTTTTGTTCCGTTTTCGGCCGGATCAGCCCTGATCTGAAAACGGATCTGTTTTCAATGCTCTGATAA
ACCGTCTCCAACCCAATTAC

Downstream 100 bases:

>100_bases
GCTATCAGCACATAAAATATCGCCAGAACCACTGGAAAATCAACGACTGTCCCATCCCCTTGAAAGGATTCAAAGTGGAA
ATCTTATGCAAACTGGAACG

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MNNSNYPEPLQPFTYPYETLQQIANDVLTLAKKGGADACEMHVSDGSGQNVTVRQGEVETIEYTRDKGLSVTVHIGHKRG
NASSSDFSPQAIRETISAALSIARYTADDIYAGLADKDLLATSFPDLDLYHPWSLSVETAIELARRCEAAALATDKRITN
SEGASVSAGASRFVYANSHGFNAGYPLSRHSISCAVIAGEQNNMQRDYWYSVARAASDLEAIEEIGQKAGMRTLARLGAR
KIATCEVPVLFESTISSGLLGYFVKAISGSSLYRKSSFLLDSIGKQVFPATIQISELPHLPKGLASCAFDDEGVATHPRK
VVENGIVQGYFLGSYTARKLGMRSTGNAGGNHNLILENDVSLPFDALLKKMNKGLLVTELLGHGVNLVTGDYSQGAAGFW
VENGEISYPVEEITIAGNLKEMLSGIVAVGDDIIVRGSRHCGSLLIERMIIAGH

Sequences:

>Translated_454_residues
MNNSNYPEPLQPFTYPYETLQQIANDVLTLAKKGGADACEMHVSDGSGQNVTVRQGEVETIEYTRDKGLSVTVHIGHKRG
NASSSDFSPQAIRETISAALSIARYTADDIYAGLADKDLLATSFPDLDLYHPWSLSVETAIELARRCEAAALATDKRITN
SEGASVSAGASRFVYANSHGFNAGYPLSRHSISCAVIAGEQNNMQRDYWYSVARAASDLEAIEEIGQKAGMRTLARLGAR
KIATCEVPVLFESTISSGLLGYFVKAISGSSLYRKSSFLLDSIGKQVFPATIQISELPHLPKGLASCAFDDEGVATHPRK
VVENGIVQGYFLGSYTARKLGMRSTGNAGGNHNLILENDVSLPFDALLKKMNKGLLVTELLGHGVNLVTGDYSQGAAGFW
VENGEISYPVEEITIAGNLKEMLSGIVAVGDDIIVRGSRHCGSLLIERMIIAGH
>Mature_454_residues
MNNSNYPEPLQPFTYPYETLQQIANDVLTLAKKGGADACEMHVSDGSGQNVTVRQGEVETIEYTRDKGLSVTVHIGHKRG
NASSSDFSPQAIRETISAALSIARYTADDIYAGLADKDLLATSFPDLDLYHPWSLSVETAIELARRCEAAALATDKRITN
SEGASVSAGASRFVYANSHGFNAGYPLSRHSISCAVIAGEQNNMQRDYWYSVARAASDLEAIEEIGQKAGMRTLARLGAR
KIATCEVPVLFESTISSGLLGYFVKAISGSSLYRKSSFLLDSIGKQVFPATIQISELPHLPKGLASCAFDDEGVATHPRK
VVENGIVQGYFLGSYTARKLGMRSTGNAGGNHNLILENDVSLPFDALLKKMNKGLLVTELLGHGVNLVTGDYSQGAAGFW
VENGEISYPVEEITIAGNLKEMLSGIVAVGDDIIVRGSRHCGSLLIERMIIAGH

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=435, Percent_Identity=52.6436781609195, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1789640, Length=402, Percent_Identity=25.3731343283582, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48786; Mature: 48786

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSNYPEPLQPFTYPYETLQQIANDVLTLAKKGGADACEMHVSDGSGQNVTVRQGEVET
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCEE
IEYTRDKGLSVTVHIGHKRGNASSSDFSPQAIRETISAALSIARYTADDIYAGLADKDLL
EEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ATSFPDLDLYHPWSLSVETAIELARRCEAAALATDKRITNSEGASVSAGASRFVYANSHG
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECCCC
FNAGYPLSRHSISCAVIAGEQNNMQRDYWYSVARAASDLEAIEEIGQKAGMRTLARLGAR
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KIATCEVPVLFESTISSGLLGYFVKAISGSSLYRKSSFLLDSIGKQVFPATIQISELPHL
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCCEEEECCCCCC
PKGLASCAFDDEGVATHPRKVVENGIVQGYFLGSYTARKLGMRSTGNAGGNHNLILENDV
CHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEECCHHHHHHCCCCCCCCCCCCCEEEECCC
SLPFDALLKKMNKGLLVTELLGHGVNLVTGDYSQGAAGFWVENGEISYPVEEITIAGNLK
CCCHHHHHHHHCCCEEHHHHHHCCCEEEECCCCCCCCEEEEECCEEECCHHHEEECCCHH
EMLSGIVAVGDDIIVRGSRHCGSLLIERMIIAGH
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNSNYPEPLQPFTYPYETLQQIANDVLTLAKKGGADACEMHVSDGSGQNVTVRQGEVET
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCEE
IEYTRDKGLSVTVHIGHKRGNASSSDFSPQAIRETISAALSIARYTADDIYAGLADKDLL
EEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ATSFPDLDLYHPWSLSVETAIELARRCEAAALATDKRITNSEGASVSAGASRFVYANSHG
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECCCC
FNAGYPLSRHSISCAVIAGEQNNMQRDYWYSVARAASDLEAIEEIGQKAGMRTLARLGAR
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KIATCEVPVLFESTISSGLLGYFVKAISGSSLYRKSSFLLDSIGKQVFPATIQISELPHL
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCCEEEECCCCCC
PKGLASCAFDDEGVATHPRKVVENGIVQGYFLGSYTARKLGMRSTGNAGGNHNLILENDV
CHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEECCHHHHHHCCCCCCCCCCCCCEEEECCC
SLPFDALLKKMNKGLLVTELLGHGVNLVTGDYSQGAAGFWVENGEISYPVEEITIAGNLK
CCCHHHHHHHHCCCEEHHHHHHCCCEEEECCCCCCCCEEEEECCEEECCHHHEEECCCHH
EMLSGIVAVGDDIIVRGSRHCGSLLIERMIIAGH
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]