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The map label for this gene is fabI [H]

Identifier: 114330678

GI number: 114330678

Start: 672032

End: 672826

Strand: Reverse

Name: fabI [H]

Synonym: Neut_0663

Alternate gene names: 114330678

Gene position: 672826-672032 (Counterclockwise)

Preceding gene: 114330680

Following gene: 114330670

Centisome position: 25.28

GC content: 50.69

Gene sequence:

>795_bases
ATGGGATTTCTAGCTGACCGGCAAATTCTGATTACAGGCCTGCTTAGCAACCGATCCATTGCTTACGGCATTGCCAAGGC
GATGCATCGCGAAGGGGCCAAACTGGCATTTACTTATCAATCAGAAGATTTACGCGAGCGCGTTGGCAAGCTAGCTGCCG
AATTTAACAGCGACCTGCTGTTTCGTTGTGATGTCAGCCAAGATGAAGAAATTACCCGCTGTTTCAGTGAATTGGCGAGC
CATTGGGATGGACTGGATGGAATCGTGCACTCCATCGCCTTCGCTCCACGCGCCGCTCTGACTGGTGATTATCTGGAAAA
CGTCGATCGGCAAGCCTTTCATATTGCACATGATATCAGTTCATACAGCTTTGCTGCACTGGCAAAAGCTGCCCTGCCGC
TGATGCAGGGAAGATCGGCCGCTTTGCTGACACTCAGCTATCTCGGCGCTGTGCGTGTGATGCCGAACTATAATGTGATG
GGGTTGGCCAAGGCGAGCCTGGAAGCTAACGTTCGCTTCATGGCGTCAAGTCTTGGTAAACAAGGGATCAGGGTGAATGC
CATCTCAGCCGGTCCAATTAAAACGCTGGCGGCAGCAGGAATTGGTAATTTTGGCAGGTTACTAGGTTATGCCGAGAAAG
TTGCACCCTTGAAAAGAAATGTGACCACCGAGGAGGTTGGTAACGTAGCTGCTTTTCTCTGCAGCAATCTGGCCAGTGGT
ATTACCGGCGAAATTACCTATGTTGATGCCGGGTTCAATACCGTCGCATTTAATCTGGACACTGATGAGCAGTAG

Upstream 100 bases:

>100_bases
GTTATACAAGGACAGCAATCCAGCAAGCTAAACCGTAAAAAAACCTCATACGGCCAAGGCACGTTTTAAGACAAACAAAT
ACTAATTGGAGTTTTCGATT

Downstream 100 bases:

>100_bases
TAGCCTGCAGGCTACTACTGCTCTTCTTATCAGTCATTCTCCTCAACTGGCTTGATTTTTACTTCGTAGCGTTGCCTGAG
GCCAGCAAGATAGGCGTTTA

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MGFLADRQILITGLLSNRSIAYGIAKAMHREGAKLAFTYQSEDLRERVGKLAAEFNSDLLFRCDVSQDEEITRCFSELAS
HWDGLDGIVHSIAFAPRAALTGDYLENVDRQAFHIAHDISSYSFAALAKAALPLMQGRSAALLTLSYLGAVRVMPNYNVM
GLAKASLEANVRFMASSLGKQGIRVNAISAGPIKTLAAAGIGNFGRLLGYAEKVAPLKRNVTTEEVGNVAAFLCSNLASG
ITGEITYVDAGFNTVAFNLDTDEQ

Sequences:

>Translated_264_residues
MGFLADRQILITGLLSNRSIAYGIAKAMHREGAKLAFTYQSEDLRERVGKLAAEFNSDLLFRCDVSQDEEITRCFSELAS
HWDGLDGIVHSIAFAPRAALTGDYLENVDRQAFHIAHDISSYSFAALAKAALPLMQGRSAALLTLSYLGAVRVMPNYNVM
GLAKASLEANVRFMASSLGKQGIRVNAISAGPIKTLAAAGIGNFGRLLGYAEKVAPLKRNVTTEEVGNVAAFLCSNLASG
ITGEITYVDAGFNTVAFNLDTDEQ
>Mature_263_residues
GFLADRQILITGLLSNRSIAYGIAKAMHREGAKLAFTYQSEDLRERVGKLAAEFNSDLLFRCDVSQDEEITRCFSELASH
WDGLDGIVHSIAFAPRAALTGDYLENVDRQAFHIAHDISSYSFAALAKAALPLMQGRSAALLTLSYLGAVRVMPNYNVMG
LAKASLEANVRFMASSLGKQGIRVNAISAGPIKTLAAAGIGNFGRLLGYAEKVAPLKRNVTTEEVGNVAAFLCSNLASGI
TGEITYVDAGFNTVAFNLDTDEQ

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031737, Length=255, Percent_Identity=28.6274509803922, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI32483357, Length=257, Percent_Identity=26.4591439688716, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=59.6899224806201, Blast_Score=321, Evalue=3e-89,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=27.3076923076923, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=27.34375, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=252, Percent_Identity=26.1904761904762, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21355319, Length=257, Percent_Identity=27.6264591439689, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28346; Mature: 28214

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLADRQILITGLLSNRSIAYGIAKAMHREGAKLAFTYQSEDLRERVGKLAAEFNSDLL
CCCCCCCCEEEEEHHCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCEE
FRCDVSQDEEITRCFSELASHWDGLDGIVHSIAFAPRAALTGDYLENVDRQAFHIAHDIS
EEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHC
SYSFAALAKAALPLMQGRSAALLTLSYLGAVRVMPNYNVMGLAKASLEANVRFMASSLGK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHHCC
QGIRVNAISAGPIKTLAAAGIGNFGRLLGYAEKVAPLKRNVTTEEVGNVAAFLCSNLASG
CCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
ITGEITYVDAGFNTVAFNLDTDEQ
CCCEEEEEECCCCEEEEECCCCCC
>Mature Secondary Structure 
GFLADRQILITGLLSNRSIAYGIAKAMHREGAKLAFTYQSEDLRERVGKLAAEFNSDLL
CCCCCCCEEEEEHHCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCEE
FRCDVSQDEEITRCFSELASHWDGLDGIVHSIAFAPRAALTGDYLENVDRQAFHIAHDIS
EEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHC
SYSFAALAKAALPLMQGRSAALLTLSYLGAVRVMPNYNVMGLAKASLEANVRFMASSLGK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHHCC
QGIRVNAISAGPIKTLAAAGIGNFGRLLGYAEKVAPLKRNVTTEEVGNVAAFLCSNLASG
CCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
ITGEITYVDAGFNTVAFNLDTDEQ
CCCEEEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]