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The map label for this gene is smpB

Identifier: 114330602

GI number: 114330602

Start: 595146

End: 595595

Strand: Direct

Name: smpB

Synonym: Neut_0587

Alternate gene names: 114330602

Gene position: 595146-595595 (Clockwise)

Preceding gene: 114330599

Following gene: 114330603

Centisome position: 22.37

GC content: 43.33

Gene sequence:

>450_bases
ATGAGCATTGCACAAAACAAAAAGGCTTTCCACGATTATTTCATTGAAGAAAAATATGAAGCCGGCATTGTGCTGGAAGG
ATGGGAGGTTAAGGCAATTCGTGCCGGTCGGGTTCAGTTAAAAGAAGCTTACATAGTTATCCGCAGGGGTGAGTTATTTC
TTATAGGATGTCATATTAGTCCGCTGCCGACAGCATCGACACATGTTAATCCTGACCCCGTAAAAACACGCAAACTGTTG
TTGCATGCTTTCCAGATCAAAGAACTCATCGGCAAAGTAGAGCGTGCGGGCTATACTCTGGTTCCCTTAGATATGCATTA
TAAAGCAGGTAAAATCAAACTGGAAATTGGCCTTGCCAAGGGAAAAAAGCAATACGATAAACGTGAAAGTGAGAAACGTA
AAGAATGGGAACGTGATAAGCAGCGCTTGTTGCGTATGCGCCATTCCTGA

Upstream 100 bases:

>100_bases
TTGTATTCTCATAGTCTGGGTGTAAAGATACAGAAAACTGATACACTAATGCGCTAATCAAATATTTTTATATGCATATA
TTTTCTTCCAGCTGTTTTGA

Downstream 100 bases:

>100_bases
TTAATATTTATGCAAACAAATTCATCTAACTCCGTTCCTTCGGCAATTTCTGATAAATATACTCAAAATCATATGCCTAT
TGCTATCTGGTTGCTGATTT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MSIAQNKKAFHDYFIEEKYEAGIVLEGWEVKAIRAGRVQLKEAYIVIRRGELFLIGCHISPLPTASTHVNPDPVKTRKLL
LHAFQIKELIGKVERAGYTLVPLDMHYKAGKIKLEIGLAKGKKQYDKRESEKRKEWERDKQRLLRMRHS

Sequences:

>Translated_149_residues
MSIAQNKKAFHDYFIEEKYEAGIVLEGWEVKAIRAGRVQLKEAYIVIRRGELFLIGCHISPLPTASTHVNPDPVKTRKLL
LHAFQIKELIGKVERAGYTLVPLDMHYKAGKIKLEIGLAKGKKQYDKRESEKRKEWERDKQRLLRMRHS
>Mature_148_residues
SIAQNKKAFHDYFIEEKYEAGIVLEGWEVKAIRAGRVQLKEAYIVIRRGELFLIGCHISPLPTASTHVNPDPVKTRKLLL
HAFQIKELIGKVERAGYTLVPLDMHYKAGKIKLEIGLAKGKKQYDKRESEKRKEWERDKQRLLRMRHS

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=147, Percent_Identity=50.3401360544218, Blast_Score=167, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_NITEC (Q0AIG7)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746824.1
- ProteinModelPortal:   Q0AIG7
- SMR:   Q0AIG7
- STRING:   Q0AIG7
- GeneID:   4272702
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0587
- NMPDR:   fig|335283.3.peg.1461
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q0AIG7
- ProtClustDB:   PRK05422
- BioCyc:   NEUT335283:NEUT_0587-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17366; Mature: 17234

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAQNKKAFHDYFIEEKYEAGIVLEGWEVKAIRAGRVQLKEAYIVIRRGELFLIGCHIS
CCCCCCHHHHHHHHHHCCCCCCEEEECCEEEEEECCCEEEEEEEEEEECCCEEEEEECCC
PLPTASTHVNPDPVKTRKLLLHAFQIKELIGKVERAGYTLVPLDMHYKAGKIKLEIGLAK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECC
GKKQYDKRESEKRKEWERDKQRLLRMRHS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIAQNKKAFHDYFIEEKYEAGIVLEGWEVKAIRAGRVQLKEAYIVIRRGELFLIGCHIS
CCCCCHHHHHHHHHHCCCCCCEEEECCEEEEEECCCEEEEEEEEEEECCCEEEEEECCC
PLPTASTHVNPDPVKTRKLLLHAFQIKELIGKVERAGYTLVPLDMHYKAGKIKLEIGLAK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECC
GKKQYDKRESEKRKEWERDKQRLLRMRHS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA