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The map label for this gene is holB [H]

Identifier: 114330561

GI number: 114330561

Start: 568731

End: 569795

Strand: Direct

Name: holB [H]

Synonym: Neut_0543

Alternate gene names: 114330561

Gene position: 568731-569795 (Clockwise)

Preceding gene: 114330554

Following gene: 114330563

Centisome position: 21.37

GC content: 50.61

Gene sequence:

>1065_bases
ATGACTGTGGCTGAAATTTTTCCATGGCAGCAGACAATCTGGCAGCAGATCCGGCAGCCGGACAGTTTGAGGCGGCATCA
TGCCCTATTGCTAAAAGGGCGCAAAGGAACTGGTAAGCTGGGTTTTGCTCTTGCTCTGGTGCGATCCATGCTGTGTGAAC
AGGTAGATGCAGCAGGGAGGGTATGCGGAAAATGTCAGGATTGTTACTGGTTTGAACAGGGGCTGCATCCGAATTTCCGT
TTGCTGGAGCCGGAAGCGTTGTCAGTTCAGGATGGGACGGCAGACAGGAATAGCGAAGAAAAGCAGGAGGAGGCTGGTGG
TACAAAGTCTGGCAAAAAGCCGAGTCAGCAGATCACGATTGCGCAGATCCGTACACTGGATGACTTTATCTATCTGAGCG
CACACCAGAATCGCTACAAGGTGGTGTTGATTCATCCGGCCGAGGCAATGAATGCAGCTGCTGCCAATGCGTTGCTGAAA
AAGCTGGAAGAACCGCCGCCAGAAGTGTTGTTTATTCTGATAGCGCACGGAACGTCAATGATTCCGGCAACTATTCTGAG
CCGCTGTCGGCAGATTGCCATGCCGACGCCGGATCGTAAACTTGCAAAAGACTGGTTGATTCAACAGGGAATTGCACAAC
CTGATTTCCGTTTGGCCATGTCGAGTTTCTCACCATTGCTGGCATTGCACTATGACGAGCAACAATCTTCGCCTCATGCT
GATTTTATTCAGAGTTTATGTGTGCCGGAGAAATTTGATCCAATCGGATTGGCAGAAAAACTGTACAAGCAGGATTTATC
CAGTGTGACCAGCTGGATGCAGAAATGGTGCTATGACCTGATGAGCTGCCACCTTAGTGGAAGGGTGCGTTACCACCTGC
AGCAGATGGATGCCATCAAGCGGCAGGCGGCAGTCATAGATCCGGTTGCGGTTGGATTTCTATGGCGTGATCTGGCTGCT
TCACAGCAACTGGCACACCACCCGCTCAACCCCAGGCTATTTCTGGAAGAGATGTTTCTGATCTGTGCTGAAATAATACA
TCCGGCAAGCTCAGAAACAAGGTAA

Upstream 100 bases:

>100_bases
TCATGCTTGTTGGAAATCGCGCTAAACTGCAATGCCTTTATTAAAGGCAGTTTCTTTATTTAATGAAAAACTATTTTTTT
AAGATAGGGTAACTTCAAAA

Downstream 100 bases:

>100_bases
TAACTACTGAATGCAGGATTCGCTGGTTGGGTCGATGGTGCAAGCCCAGAGCGTTCTGTGAATACCTGCTGCACGATTAA
ACAGGTGGTCGGCCTGTTGG

Product: DNA polymerase III, delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTVAEIFPWQQTIWQQIRQPDSLRRHHALLLKGRKGTGKLGFALALVRSMLCEQVDAAGRVCGKCQDCYWFEQGLHPNFR
LLEPEALSVQDGTADRNSEEKQEEAGGTKSGKKPSQQITIAQIRTLDDFIYLSAHQNRYKVVLIHPAEAMNAAAANALLK
KLEEPPPEVLFILIAHGTSMIPATILSRCRQIAMPTPDRKLAKDWLIQQGIAQPDFRLAMSSFSPLLALHYDEQQSSPHA
DFIQSLCVPEKFDPIGLAEKLYKQDLSSVTSWMQKWCYDLMSCHLSGRVRYHLQQMDAIKRQAAVIDPVAVGFLWRDLAA
SQQLAHHPLNPRLFLEEMFLICAEIIHPASSETR

Sequences:

>Translated_354_residues
MTVAEIFPWQQTIWQQIRQPDSLRRHHALLLKGRKGTGKLGFALALVRSMLCEQVDAAGRVCGKCQDCYWFEQGLHPNFR
LLEPEALSVQDGTADRNSEEKQEEAGGTKSGKKPSQQITIAQIRTLDDFIYLSAHQNRYKVVLIHPAEAMNAAAANALLK
KLEEPPPEVLFILIAHGTSMIPATILSRCRQIAMPTPDRKLAKDWLIQQGIAQPDFRLAMSSFSPLLALHYDEQQSSPHA
DFIQSLCVPEKFDPIGLAEKLYKQDLSSVTSWMQKWCYDLMSCHLSGRVRYHLQQMDAIKRQAAVIDPVAVGFLWRDLAA
SQQLAHHPLNPRLFLEEMFLICAEIIHPASSETR
>Mature_353_residues
TVAEIFPWQQTIWQQIRQPDSLRRHHALLLKGRKGTGKLGFALALVRSMLCEQVDAAGRVCGKCQDCYWFEQGLHPNFRL
LEPEALSVQDGTADRNSEEKQEEAGGTKSGKKPSQQITIAQIRTLDDFIYLSAHQNRYKVVLIHPAEAMNAAAANALLKK
LEEPPPEVLFILIAHGTSMIPATILSRCRQIAMPTPDRKLAKDWLIQQGIAQPDFRLAMSSFSPLLALHYDEQQSSPHAD
FIQSLCVPEKFDPIGLAEKLYKQDLSSVTSWMQKWCYDLMSCHLSGRVRYHLQQMDAIKRQAAVIDPVAVGFLWRDLAAS
QQLAHHPLNPRLFLEEMFLICAEIIHPASSETR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=175, Percent_Identity=30.8571428571429, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787341, Length=204, Percent_Identity=28.921568627451, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40002; Mature: 39870

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAEIFPWQQTIWQQIRQPDSLRRHHALLLKGRKGTGKLGFALALVRSMLCEQVDAAGR
CCCHHHCCHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
VCGKCQDCYWFEQGLHPNFRLLEPEALSVQDGTADRNSEEKQEEAGGTKSGKKPSQQITI
HHHCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHH
AQIRTLDDFIYLSAHQNRYKVVLIHPAEAMNAAAANALLKKLEEPPPEVLFILIAHGTSM
HHHHHHHHHEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCC
IPATILSRCRQIAMPTPDRKLAKDWLIQQGIAQPDFRLAMSSFSPLLALHYDEQQSSPHA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEECHHCCCCHH
DFIQSLCVPEKFDPIGLAEKLYKQDLSSVTSWMQKWCYDLMSCHLSGRVRYHLQQMDAIK
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RQAAVIDPVAVGFLWRDLAASQQLAHHPLNPRLFLEEMFLICAEIIHPASSETR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVAEIFPWQQTIWQQIRQPDSLRRHHALLLKGRKGTGKLGFALALVRSMLCEQVDAAGR
CCHHHCCHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
VCGKCQDCYWFEQGLHPNFRLLEPEALSVQDGTADRNSEEKQEEAGGTKSGKKPSQQITI
HHHCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHH
AQIRTLDDFIYLSAHQNRYKVVLIHPAEAMNAAAANALLKKLEEPPPEVLFILIAHGTSM
HHHHHHHHHEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCC
IPATILSRCRQIAMPTPDRKLAKDWLIQQGIAQPDFRLAMSSFSPLLALHYDEQQSSPHA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEECHHCCCCHH
DFIQSLCVPEKFDPIGLAEKLYKQDLSSVTSWMQKWCYDLMSCHLSGRVRYHLQQMDAIK
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RQAAVIDPVAVGFLWRDLAASQQLAHHPLNPRLFLEEMFLICAEIIHPASSETR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]