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The map label for this gene is dapF

Identifier: 114330524

GI number: 114330524

Start: 525002

End: 525838

Strand: Reverse

Name: dapF

Synonym: Neut_0505

Alternate gene names: 114330524

Gene position: 525838-525002 (Counterclockwise)

Preceding gene: 114330537

Following gene: 114330523

Centisome position: 19.76

GC content: 51.61

Gene sequence:

>837_bases
ATGAAATTACAATTTACGAAAATGCACGGGCTGGGAAATGATTTCATTGTCATCAATGCAATCAACCAGCCAGCCAGCCT
CACTTTCCTGGATCCTGCCACGATTCGCCGGCTGGCAGATCGTCATTTCGGCATTGGGTTCGATCAACTACTGATTGTGG
AACAGGCCAGGGAAGGTGGAGATTTTCGCTATCGTATCTTCAACGCCGATGGTGGAGAAGTTGAACAATGCGGCAATGGC
GCCCGCTGCTTTGCCCGCTTTGTGCGTGACTACAATCTGACTGACAAAAATACGATCCGCGTGGAAACCGCTCGCGGCAT
CATCACGCCGACGATCGAAAACAATGGTGAAGTTTCCGTCAATATGGGAGTTCCCCAGTTTGAACCGGCTGAGATTCCTT
TTCAGGCTGCACAGCGTATGCCTGTTTACCCATTGCAAATCGGCGACAAAACCATTGAAATCTCTGCTGTCTCGATAGGG
AACCCTCATGCCGTGCAAATCATTCCAGATATTGATCTGGCACCGGTCACAACTGAAGGCCCCAAAATTGAAGCCCACCC
ACTTTTTCCTGAAAGAGTTAACGCCGGCTTTATGCAGATTATTGACCGCGCGCATATCCGGCTACGTGTTTTTGAACGCG
GAACCGGCGAAACATTGGCCTGCGGTACCGGCGCCTGTGCTGCCGTTGTCTGCGGCATTCTGCGCGGCCTTCTGGATACG
ACAGTACAGGTAGCCATGCACGGCGGCAATCTCCAGATTCGCTGGGACGGAAAAGACAAGCCTGTCTGGATGACCGGGCC
GGCTATTACCGTATTTGAAGGCACAATTGATCTGTAA

Upstream 100 bases:

>100_bases
CAAGCAGGAAAATATGACATTTGTTCCCGGGATTGAATTACATCCATCAGCCAACTCAGAACATCTACACACCTTCTGAT
AAACCTGAAGGACTCTAAAA

Downstream 100 bases:

>100_bases
ACACCCACTCATCCTATCATGCGCACGCTGATTACTTACATCATGGTCTTCCCGAAGCGCAGCGCATTCGTACTGATTGC
TCTGCTGCTGGCAGGCGTCG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKLQFTKMHGLGNDFIVINAINQPASLTFLDPATIRRLADRHFGIGFDQLLIVEQAREGGDFRYRIFNADGGEVEQCGNG
ARCFARFVRDYNLTDKNTIRVETARGIITPTIENNGEVSVNMGVPQFEPAEIPFQAAQRMPVYPLQIGDKTIEISAVSIG
NPHAVQIIPDIDLAPVTTEGPKIEAHPLFPERVNAGFMQIIDRAHIRLRVFERGTGETLACGTGACAAVVCGILRGLLDT
TVQVAMHGGNLQIRWDGKDKPVWMTGPAITVFEGTIDL

Sequences:

>Translated_278_residues
MKLQFTKMHGLGNDFIVINAINQPASLTFLDPATIRRLADRHFGIGFDQLLIVEQAREGGDFRYRIFNADGGEVEQCGNG
ARCFARFVRDYNLTDKNTIRVETARGIITPTIENNGEVSVNMGVPQFEPAEIPFQAAQRMPVYPLQIGDKTIEISAVSIG
NPHAVQIIPDIDLAPVTTEGPKIEAHPLFPERVNAGFMQIIDRAHIRLRVFERGTGETLACGTGACAAVVCGILRGLLDT
TVQVAMHGGNLQIRWDGKDKPVWMTGPAITVFEGTIDL
>Mature_278_residues
MKLQFTKMHGLGNDFIVINAINQPASLTFLDPATIRRLADRHFGIGFDQLLIVEQAREGGDFRYRIFNADGGEVEQCGNG
ARCFARFVRDYNLTDKNTIRVETARGIITPTIENNGEVSVNMGVPQFEPAEIPFQAAQRMPVYPLQIGDKTIEISAVSIG
NPHAVQIIPDIDLAPVTTEGPKIEAHPLFPERVNAGFMQIIDRAHIRLRVFERGTGETLACGTGACAAVVCGILRGLLDT
TVQVAMHGGNLQIRWDGKDKPVWMTGPAITVFEGTIDL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=277, Percent_Identity=55.5956678700361, Blast_Score=311, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_NITEC (Q0AIP0)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746746.1
- ProteinModelPortal:   Q0AIP0
- SMR:   Q0AIP0
- STRING:   Q0AIP0
- GeneID:   4272006
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0505
- NMPDR:   fig|335283.3.peg.1382
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   Q0AIP0
- ProtClustDB:   PRK00450
- BioCyc:   NEUT335283:NEUT_0505-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30467; Mature: 30467

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 77-77 ACT_SITE 221-221

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQFTKMHGLGNDFIVINAINQPASLTFLDPATIRRLADRHFGIGFDQLLIVEQAREGG
CCEEEEECCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHCCCCHHHEEEEEECCCCC
DFRYRIFNADGGEVEQCGNGARCFARFVRDYNLTDKNTIRVETARGIITPTIENNGEVSV
CEEEEEEECCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEE
NMGVPQFEPAEIPFQAAQRMPVYPLQIGDKTIEISAVSIGNPHAVQIIPDIDLAPVTTEG
EECCCCCCCCCCCHHHHCCCCEEEEEECCEEEEEEEEECCCCEEEEEECCCCCCCCCCCC
PKIEAHPLFPERVNAGFMQIIDRAHIRLRVFERGTGETLACGTGACAAVVCGILRGLLDT
CCEEECCCCHHHHCCHHHHHHHCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHH
TVQVAMHGGNLQIRWDGKDKPVWMTGPAITVFEGTIDL
EEEEEEECCEEEEEECCCCCCEEEECCEEEEEEEEECC
>Mature Secondary Structure
MKLQFTKMHGLGNDFIVINAINQPASLTFLDPATIRRLADRHFGIGFDQLLIVEQAREGG
CCEEEEECCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHCCCCHHHEEEEEECCCCC
DFRYRIFNADGGEVEQCGNGARCFARFVRDYNLTDKNTIRVETARGIITPTIENNGEVSV
CEEEEEEECCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEE
NMGVPQFEPAEIPFQAAQRMPVYPLQIGDKTIEISAVSIGNPHAVQIIPDIDLAPVTTEG
EECCCCCCCCCCCHHHHCCCCEEEEEECCEEEEEEEEECCCCEEEEEECCCCCCCCCCCC
PKIEAHPLFPERVNAGFMQIIDRAHIRLRVFERGTGETLACGTGACAAVVCGILRGLLDT
CCEEECCCCHHHHCCHHHHHHHCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHH
TVQVAMHGGNLQIRWDGKDKPVWMTGPAITVFEGTIDL
EEEEEEECCEEEEEECCCCCCEEEECCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA