Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 114330520

GI number: 114330520

Start: 518591

End: 519934

Strand: Direct

Name: gdhA [H]

Synonym: Neut_0501

Alternate gene names: 114330520

Gene position: 518591-519934 (Clockwise)

Preceding gene: 114330519

Following gene: 114330521

Centisome position: 19.49

GC content: 50.3

Gene sequence:

>1344_bases
ATGAAATACAGCAGTATCGAGGAATTCAAGAATTATGTTTCTGAAAGGAATCCGGGGCAACCGGAATTCCTGCAGGCTGT
TTCAGAAGTCATTGAAAGTTTGTGGCCTTTTATCGTTGATCATTCCCGTTACGCTGAGCAGGGGTTGCTGGACCGGCTGA
TTGAACCGGAACGCATGATTGTATTTCGTGTGGCGTGGGTGGATGATCGTAGCGAAGTCAGGGTTAACCGAGGATACCGC
ATTCAATATAACTCCGCGATCGGGCCATACAAGGGAGGGACGCGGTTCCACCCCTCCGTCAATCTTTCCATTCTCAAGTT
TCTTGCTTTTGAGCAAACCTTTAAAAACGCATTGACAACTTTACCTATGGGTGGAGGTAAAGGTGGATCAGATTTTGATC
CCAAGGGTAAAAGTCCCGGGGAAGTCATGCGCTTCTGTCAGGCGTATGCGACTGAGTTGTTCCGGCATGTCGGGGTGGAT
ACAGATGTACCCGCCGGGGATATTGGCGTGGGTGGCAGGGAAGTCGGTTATATGGCTGGCATGGTGAAGAAACTGACTAA
CCGGTCGGATTGCGTGTTTACCGGCAAAGGATTGAGTTTTGGCGGGTCATTGCTGCGGCCGGAGGCGACCGGGTATGGGC
TGGTCTATTTTGCCGAAGAGATGCTGAATCATGTTGGCCGATCGTTGAAGGACATGCGTGTATCTGTGTCCGGCTCGGGA
AATGTGGCACAGTTTGCCATTGACAAAGCCATGTCACTGGGAGCCAGAGTGATCACCGTTTCCGATTCGAGTGGAACAGT
GGTGGATGAAGCCGGATTTACACCAGAGAAATTGGCGATTCTGGCTGAAGTCAAGAATCATTCTTACGGACGTGTAAACG
AGTATGCTGAAAAGGTAGGCGCACAATTCCTTTCATGTGAAAAACCATGGAATGTACCGGTGGATGTCGCTTTGCCTTGT
GCGACGCAGAACGAGTTGAATGATAATGATGCCGCAATACTGGTCAAGAATGGGGTGAGTTGTGTGGCTGAGGGTGCCAA
TATGCCTTGTACTGCGGGGGCAATCGAACGGTTCCAGTATGCAAAAATACTGTTTGCGCCTGGTAAGGCGAGCAATGCCG
GAGGGGTAGCTACCTCAGGCTTGGAGATGAGCCAACAGGCCATGCGGCTTTCCTGGACAAGTGGCGAGGTGGATATGCGC
TTGCATGAAATTATGCGTGCTATTCACCGTTCCTGTATCGGGTACGGCCAGAAAAATAATGGCATAGTCAATTATGTGGA
TGGCGCGAATATAGCCGGTTTTGTGAAAGTAGCGGAAGCAATGCTGGCACAAGGCGTTATTTGA

Upstream 100 bases:

>100_bases
TCTATGAAATTTTGCGATGATTATCGGCGTAATGTAGGGATGCGGTGGCCGATTTTTTTGTGCGTTTGTCTGGAATATTA
TTTACTGAGGGGAATGGAAA

Downstream 100 bases:

>100_bases
TCACGAAAAGCGCTATACATCTTTGTGAATTGATGGCTCTGGCAGCAATAGATGAACGATGCTCCCTCTTATTGCAACAT
GAATGTGTTGTTTCCCCTGT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKYSSIEEFKNYVSERNPGQPEFLQAVSEVIESLWPFIVDHSRYAEQGLLDRLIEPERMIVFRVAWVDDRSEVRVNRGYR
IQYNSAIGPYKGGTRFHPSVNLSILKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSPGEVMRFCQAYATELFRHVGVD
TDVPAGDIGVGGREVGYMAGMVKKLTNRSDCVFTGKGLSFGGSLLRPEATGYGLVYFAEEMLNHVGRSLKDMRVSVSGSG
NVAQFAIDKAMSLGARVITVSDSSGTVVDEAGFTPEKLAILAEVKNHSYGRVNEYAEKVGAQFLSCEKPWNVPVDVALPC
ATQNELNDNDAAILVKNGVSCVAEGANMPCTAGAIERFQYAKILFAPGKASNAGGVATSGLEMSQQAMRLSWTSGEVDMR
LHEIMRAIHRSCIGYGQKNNGIVNYVDGANIAGFVKVAEAMLAQGVI

Sequences:

>Translated_447_residues
MKYSSIEEFKNYVSERNPGQPEFLQAVSEVIESLWPFIVDHSRYAEQGLLDRLIEPERMIVFRVAWVDDRSEVRVNRGYR
IQYNSAIGPYKGGTRFHPSVNLSILKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSPGEVMRFCQAYATELFRHVGVD
TDVPAGDIGVGGREVGYMAGMVKKLTNRSDCVFTGKGLSFGGSLLRPEATGYGLVYFAEEMLNHVGRSLKDMRVSVSGSG
NVAQFAIDKAMSLGARVITVSDSSGTVVDEAGFTPEKLAILAEVKNHSYGRVNEYAEKVGAQFLSCEKPWNVPVDVALPC
ATQNELNDNDAAILVKNGVSCVAEGANMPCTAGAIERFQYAKILFAPGKASNAGGVATSGLEMSQQAMRLSWTSGEVDMR
LHEIMRAIHRSCIGYGQKNNGIVNYVDGANIAGFVKVAEAMLAQGVI
>Mature_447_residues
MKYSSIEEFKNYVSERNPGQPEFLQAVSEVIESLWPFIVDHSRYAEQGLLDRLIEPERMIVFRVAWVDDRSEVRVNRGYR
IQYNSAIGPYKGGTRFHPSVNLSILKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSPGEVMRFCQAYATELFRHVGVD
TDVPAGDIGVGGREVGYMAGMVKKLTNRSDCVFTGKGLSFGGSLLRPEATGYGLVYFAEEMLNHVGRSLKDMRVSVSGSG
NVAQFAIDKAMSLGARVITVSDSSGTVVDEAGFTPEKLAILAEVKNHSYGRVNEYAEKVGAQFLSCEKPWNVPVDVALPC
ATQNELNDNDAAILVKNGVSCVAEGANMPCTAGAIERFQYAKILFAPGKASNAGGVATSGLEMSQQAMRLSWTSGEVDMR
LHEIMRAIHRSCIGYGQKNNGIVNYVDGANIAGFVKVAEAMLAQGVI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=374, Percent_Identity=28.3422459893048, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI4885281, Length=374, Percent_Identity=27.5401069518717, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1788059, Length=443, Percent_Identity=69.0744920993228, Blast_Score=638, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=341, Percent_Identity=26.9794721407625, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324951, Length=449, Percent_Identity=48.9977728285078, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6319256, Length=451, Percent_Identity=48.1152993348115, Blast_Score=383, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24649283, Length=353, Percent_Identity=30.028328611898, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45553475, Length=371, Percent_Identity=28.8409703504043, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45549226, Length=371, Percent_Identity=28.8409703504043, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24649460, Length=297, Percent_Identity=29.2929292929293, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48479; Mature: 48479

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYSSIEEFKNYVSERNPGQPEFLQAVSEVIESLWPFIVDHSRYAEQGLLDRLIEPERMI
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEE
VFRVAWVDDRSEVRVNRGYRIQYNSAIGPYKGGTRFHPSVNLSILKFLAFEQTFKNALTT
EEEEEEECCCCCEEECCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHC
LPMGGGKGGSDFDPKGKSPGEVMRFCQAYATELFRHVGVDTDVPAGDIGVGGREVGYMAG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
MVKKLTNRSDCVFTGKGLSFGGSLLRPEATGYGLVYFAEEMLNHVGRSLKDMRVSVSGSG
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCEEEECCCC
NVAQFAIDKAMSLGARVITVSDSSGTVVDEAGFTPEKLAILAEVKNHSYGRVNEYAEKVG
CCHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
AQFLSCEKPWNVPVDVALPCATQNELNDNDAAILVKNGVSCVAEGANMPCTAGAIERFQY
HHHHCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHE
AKILFAPGKASNAGGVATSGLEMSQQAMRLSWTSGEVDMRLHEIMRAIHRSCIGYGQKNN
EEEEECCCCCCCCCCCEECCHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GIVNYVDGANIAGFVKVAEAMLAQGVI
CEEEECCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKYSSIEEFKNYVSERNPGQPEFLQAVSEVIESLWPFIVDHSRYAEQGLLDRLIEPERMI
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEE
VFRVAWVDDRSEVRVNRGYRIQYNSAIGPYKGGTRFHPSVNLSILKFLAFEQTFKNALTT
EEEEEEECCCCCEEECCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHC
LPMGGGKGGSDFDPKGKSPGEVMRFCQAYATELFRHVGVDTDVPAGDIGVGGREVGYMAG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
MVKKLTNRSDCVFTGKGLSFGGSLLRPEATGYGLVYFAEEMLNHVGRSLKDMRVSVSGSG
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCEEEECCCC
NVAQFAIDKAMSLGARVITVSDSSGTVVDEAGFTPEKLAILAEVKNHSYGRVNEYAEKVG
CCHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
AQFLSCEKPWNVPVDVALPCATQNELNDNDAAILVKNGVSCVAEGANMPCTAGAIERFQY
HHHHCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHE
AKILFAPGKASNAGGVATSGLEMSQQAMRLSWTSGEVDMRLHEIMRAIHRSCIGYGQKNN
EEEEECCCCCCCCCCCEECCHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GIVNYVDGANIAGFVKVAEAMLAQGVI
CEEEECCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]