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The map label for this gene is ybiS [H]

Identifier: 114330477

GI number: 114330477

Start: 470249

End: 471220

Strand: Reverse

Name: ybiS [H]

Synonym: Neut_0457

Alternate gene names: 114330477

Gene position: 471220-470249 (Counterclockwise)

Preceding gene: 114330484

Following gene: 114330465

Centisome position: 17.71

GC content: 46.19

Gene sequence:

>972_bases
ATGAAACAACAAAATATACAGACACTTAGAATCAGCTTATTGCTACTGTTCATGATGTTGTCACTCATGCCTTGTGTTTA
TGCTGGCAGCTGGGTACGCCCACCCGATGACATAGACATTCTTGGGCAAATTCAGACAGTCACAGCCAGTCGCTCTGAAA
CTCTACTCGATGTGGCGCGCCGTTATGACATTGGCCAAGATGAAATGATGCTGGCCAACCCCAATGTAAATCGCTGGCTG
CCTGAAGAAGGGGCAAAAGTTATTTTGCCACTGCGCTTTATCATTCCTCAGGCTGAACGTACCGGACTTGTAATAAATCT
ACCAGAAATGCGGCTTTATTATTTCCCCAAACCAATAAAAGGGCAGAAACCAGAGATTATTACTCATCCTGTCAGTATTG
GCAGGATGGATTGGAACACACCGCTGGGCAAAACCACTATCGTCAGAAAACAAAAAGACCCTACATGGACCCCCCCCCAA
TCACTCCGGAAAGAAGCAATAGAGGAAGGCAGACCTCCGTTATTGGATGTCGTTCCAGCCGGACCGGACAACCCTCTCGG
GAAATATGCACTCTATCTGGGCTTGCCTGGATACCTCATCCACGGCACCAACAAGCCGCTTGGTGTAGGGATGCGTGTCA
CACATGGCTGCATGCGTCTATATCCGGAAGATATCGAGGAACTGTTTAATCTTATTCCTACAGGCACGCCGGTACAAATA
GTCAATCAGCCTATCAAACTGGGATGGCAGGGTGATCTCTTATACATCGAGCTTCACCCACCACTGGAGGAAGAAAATAT
TACACCTCTCGACTTTGAACAGGAAATCCATCGCACAATTTTGGAATTCTTTGAAAGAACATCGAAGGATACTGACGGTA
GAATGACACGGAATATCAAAATCGACCAACAAGCTCTGGAGTCAGCAATTCAAGCCAGAGATGGTATACCCACTTTAATT
TCTGAAGATTAA

Upstream 100 bases:

>100_bases
TCTAAATATTCGGCCAGTATAGGTAAAATAACCAACCTGCTATTCAAACGGAAATAAACTTAATGATGCTATCACCCTCA
GGCAAACAACACGATATAAA

Downstream 100 bases:

>100_bases
CAAGAGACTGATACTCAAATATATTTAACATCCAATATTTCATATTCCCGGATACCACCAGGAGCTTGAACTTCAGCCAC
ATCTCCCGCATATTTACCAA

Product: ErfK/YbiS/YcfS/YnhG family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKQQNIQTLRISLLLLFMMLSLMPCVYAGSWVRPPDDIDILGQIQTVTASRSETLLDVARRYDIGQDEMMLANPNVNRWL
PEEGAKVILPLRFIIPQAERTGLVINLPEMRLYYFPKPIKGQKPEIITHPVSIGRMDWNTPLGKTTIVRKQKDPTWTPPQ
SLRKEAIEEGRPPLLDVVPAGPDNPLGKYALYLGLPGYLIHGTNKPLGVGMRVTHGCMRLYPEDIEELFNLIPTGTPVQI
VNQPIKLGWQGDLLYIELHPPLEEENITPLDFEQEIHRTILEFFERTSKDTDGRMTRNIKIDQQALESAIQARDGIPTLI
SED

Sequences:

>Translated_323_residues
MKQQNIQTLRISLLLLFMMLSLMPCVYAGSWVRPPDDIDILGQIQTVTASRSETLLDVARRYDIGQDEMMLANPNVNRWL
PEEGAKVILPLRFIIPQAERTGLVINLPEMRLYYFPKPIKGQKPEIITHPVSIGRMDWNTPLGKTTIVRKQKDPTWTPPQ
SLRKEAIEEGRPPLLDVVPAGPDNPLGKYALYLGLPGYLIHGTNKPLGVGMRVTHGCMRLYPEDIEELFNLIPTGTPVQI
VNQPIKLGWQGDLLYIELHPPLEEENITPLDFEQEIHRTILEFFERTSKDTDGRMTRNIKIDQQALESAIQARDGIPTLI
SED
>Mature_323_residues
MKQQNIQTLRISLLLLFMMLSLMPCVYAGSWVRPPDDIDILGQIQTVTASRSETLLDVARRYDIGQDEMMLANPNVNRWL
PEEGAKVILPLRFIIPQAERTGLVINLPEMRLYYFPKPIKGQKPEIITHPVSIGRMDWNTPLGKTTIVRKQKDPTWTPPQ
SLRKEAIEEGRPPLLDVVPAGPDNPLGKYALYLGLPGYLIHGTNKPLGVGMRVTHGCMRLYPEDIEELFNLIPTGTPVQI
VNQPIKLGWQGDLLYIELHPPLEEENITPLDFEQEIHRTILEFFERTSKDTDGRMTRNIKIDQQALESAIQARDGIPTLI
SED

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=313, Percent_Identity=38.6581469648562, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1788299, Length=318, Percent_Identity=36.1635220125786, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1787968, Length=324, Percent_Identity=35.8024691358025, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787356, Length=294, Percent_Identity=34.0136054421769, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 36698; Mature: 36698

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQNIQTLRISLLLLFMMLSLMPCVYAGSWVRPPDDIDILGQIQTVTASRSETLLDVAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCHHHHHHHHH
RYDIGQDEMMLANPNVNRWLPEEGAKVILPLRFIIPQAERTGLVINLPEMRLYYFPKPIK
HCCCCCCCEEEECCCCCCCCCCCCCEEEEEHHEECCCCCCCCEEEECCCCEEEECCCCCC
GQKPEIITHPVSIGRMDWNTPLGKTTIVRKQKDPTWTPPQSLRKEAIEEGRPPLLDVVPA
CCCCCEEECCCEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECC
GPDNPLGKYALYLGLPGYLIHGTNKPLGVGMRVTHGCMRLYPEDIEELFNLIPTGTPVQI
CCCCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHH
VNQPIKLGWQGDLLYIELHPPLEEENITPLDFEQEIHRTILEFFERTSKDTDGRMTRNIK
HCCCEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
IDQQALESAIQARDGIPTLISED
ECHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKQQNIQTLRISLLLLFMMLSLMPCVYAGSWVRPPDDIDILGQIQTVTASRSETLLDVAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCHHHHHHHHH
RYDIGQDEMMLANPNVNRWLPEEGAKVILPLRFIIPQAERTGLVINLPEMRLYYFPKPIK
HCCCCCCCEEEECCCCCCCCCCCCCEEEEEHHEECCCCCCCCEEEECCCCEEEECCCCCC
GQKPEIITHPVSIGRMDWNTPLGKTTIVRKQKDPTWTPPQSLRKEAIEEGRPPLLDVVPA
CCCCCEEECCCEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECC
GPDNPLGKYALYLGLPGYLIHGTNKPLGVGMRVTHGCMRLYPEDIEELFNLIPTGTPVQI
CCCCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHH
VNQPIKLGWQGDLLYIELHPPLEEENITPLDFEQEIHRTILEFFERTSKDTDGRMTRNIK
HCCCEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
IDQQALESAIQARDGIPTLISED
ECHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]