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The map label for this gene is carA [H]

Identifier: 114330474

GI number: 114330474

Start: 465199

End: 466350

Strand: Direct

Name: carA [H]

Synonym: Neut_0454

Alternate gene names: 114330474

Gene position: 465199-466350 (Clockwise)

Preceding gene: 114330473

Following gene: 114330475

Centisome position: 17.48

GC content: 48.18

Gene sequence:

>1152_bases
GTGTCTAACCGTCCGCGCGCTGTACTCGTTTTGGCTGATGGTACTGCTTTTCATGGGGACTCAATTGGTGCCGAAGGTGT
GGCTACCGGGGAAGTGGCCTTCAATACGGCTATGACCGGCTACCAGGAAATTCTTACCGATCCGTCTTACTGCCGTCAGA
TTGTTACACTGACTTATCCGCATATTGGCAATACCGGTGTTAATGACGAAGATATTGAATCTACGATAGATTCCAAAGTC
ATTCATGTGGCAGGGTTGGTGATTCGAGATTTGCCGCTCATTGCCAGCAGTTGGCGCAGTCGCCAATTATTGCCAGATTT
TCTCAAGCAGCATAATGTTCCAGCGATTGCCGAGATCGATACTCGTAAGCTCACACGTATTTTAAGAATCAAAGGTGCTC
AGGCAGGTTGCATCATGGCTGGTCATATCGATGAAATCGAGGCTTTACAACAAGCACGGACTTTTCCCGGTCTGACTGGA
ATGGATCTGGCGCGCGTGGTTAGCTGTAACCAATCATGGGAATTCAGTGAAGGGGAATGGGATCCGAAAACAGGCTATCA
GACTGTAAGTGAGCGTCAATACCGGGTTGTTGTGTTGGATTTTGGTGTCAAGCGTACGATTTTGCGTAAACTAGTTGAGC
GAGGTTGTCACGTTATTGTCTATCCTGCTCAAACGCGGATTGAGGAGATTCTTGCTGCCAAACCTGATGGAGTAGTGCTT
TCCAATGGCCCGGGTGATCCGGAAGCCTGTGGTTATGCGATTGCTACTACACGTGCATTGCTGGAAAGTGGAGTTCCTGT
ATTTGGAATTTGTCTGGGCCATCAGCTGGTTGCTTTAGCGATTGGTGCCAAAACGATCAAGATGCGATTTGGTCATCATG
GTGCTAATCACCCGGTGTTGGATGTTGATAGCAATCGGGTGATGATTACCAGCCAGAATCACGGGTTTGCAGTCGCTACC
GACACCTTACCTGCTAATGCACGCGTTACTCATCATTCATTGTTCGACGGGAGTCTTCAGGGTTTCGAACTGACAGATCA
TCCTGTTTTCTGTTTCCAAGGACATCCTGAAGCAAGTCCAGGTCCTCAGGATGTTGATTATTTATTCAGTCGTTTCGTCA
GTCGGATGGCGGAAAACAAGCAACCAGCCTAA

Upstream 100 bases:

>100_bases
CTCCCGTACTCATGTATAATTAACTTTAGCGGTGCGGAATCAATTCGAACCGCTTTTTTTCTGTCTGTCTGCGTTTTGCT
ACAAATCATTGGGGAGTCCT

Downstream 100 bases:

>100_bases
GTCATCAGAACAATCTTCTACTTAAATGCCTAAGCGTACCGATCTGAAATCCATCCTCATTATTGGTGCCGGGCCGATTG
TTATCGGTCAGGCATGTGAA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSNRPRAVLVLADGTAFHGDSIGAEGVATGEVAFNTAMTGYQEILTDPSYCRQIVTLTYPHIGNTGVNDEDIESTIDSKV
IHVAGLVIRDLPLIASSWRSRQLLPDFLKQHNVPAIAEIDTRKLTRILRIKGAQAGCIMAGHIDEIEALQQARTFPGLTG
MDLARVVSCNQSWEFSEGEWDPKTGYQTVSERQYRVVVLDFGVKRTILRKLVERGCHVIVYPAQTRIEEILAAKPDGVVL
SNGPGDPEACGYAIATTRALLESGVPVFGICLGHQLVALAIGAKTIKMRFGHHGANHPVLDVDSNRVMITSQNHGFAVAT
DTLPANARVTHHSLFDGSLQGFELTDHPVFCFQGHPEASPGPQDVDYLFSRFVSRMAENKQPA

Sequences:

>Translated_383_residues
MSNRPRAVLVLADGTAFHGDSIGAEGVATGEVAFNTAMTGYQEILTDPSYCRQIVTLTYPHIGNTGVNDEDIESTIDSKV
IHVAGLVIRDLPLIASSWRSRQLLPDFLKQHNVPAIAEIDTRKLTRILRIKGAQAGCIMAGHIDEIEALQQARTFPGLTG
MDLARVVSCNQSWEFSEGEWDPKTGYQTVSERQYRVVVLDFGVKRTILRKLVERGCHVIVYPAQTRIEEILAAKPDGVVL
SNGPGDPEACGYAIATTRALLESGVPVFGICLGHQLVALAIGAKTIKMRFGHHGANHPVLDVDSNRVMITSQNHGFAVAT
DTLPANARVTHHSLFDGSLQGFELTDHPVFCFQGHPEASPGPQDVDYLFSRFVSRMAENKQPA
>Mature_382_residues
SNRPRAVLVLADGTAFHGDSIGAEGVATGEVAFNTAMTGYQEILTDPSYCRQIVTLTYPHIGNTGVNDEDIESTIDSKVI
HVAGLVIRDLPLIASSWRSRQLLPDFLKQHNVPAIAEIDTRKLTRILRIKGAQAGCIMAGHIDEIEALQQARTFPGLTGM
DLARVVSCNQSWEFSEGEWDPKTGYQTVSERQYRVVVLDFGVKRTILRKLVERGCHVIVYPAQTRIEEILAAKPDGVVLS
NGPGDPEACGYAIATTRALLESGVPVFGICLGHQLVALAIGAKTIKMRFGHHGANHPVLDVDSNRVMITSQNHGFAVATD
TLPANARVTHHSLFDGSLQGFELTDHPVFCFQGHPEASPGPQDVDYLFSRFVSRMAENKQPA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=381, Percent_Identity=39.3700787401575, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI21361331, Length=382, Percent_Identity=34.8167539267016, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI169790915, Length=382, Percent_Identity=34.8167539267016, Blast_Score=208, Evalue=6e-54,
Organism=Escherichia coli, GI1786215, Length=381, Percent_Identity=60.6299212598425, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI193204318, Length=384, Percent_Identity=35.15625, Blast_Score=217, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6324878, Length=384, Percent_Identity=35.9375, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6322331, Length=409, Percent_Identity=35.2078239608802, Blast_Score=219, Evalue=4e-58,
Organism=Drosophila melanogaster, GI45555749, Length=386, Percent_Identity=38.0829015544041, Blast_Score=234, Evalue=7e-62,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=38.0829015544041, Blast_Score=234, Evalue=9e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41729; Mature: 41598

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRPRAVLVLADGTAFHGDSIGAEGVATGEVAFNTAMTGYQEILTDPSYCRQIVTLTYP
CCCCCCEEEEEECCCEECCCCCCCCCEEECCEEHHHHHHHHHHHHCCHHHHHHHHHEECC
HIGNTGVNDEDIESTIDSKVIHVAGLVIRDLPLIASSWRSRQLLPDFLKQHNVPAIAEID
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECC
TRKLTRILRIKGAQAGCIMAGHIDEIEALQQARTFPGLTGMDLARVVSCNQSWEFSEGEW
HHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCC
DPKTGYQTVSERQYRVVVLDFGVKRTILRKLVERGCHVIVYPAQTRIEEILAAKPDGVVL
CCCCCCHHHCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEE
SNGPGDPEACGYAIATTRALLESGVPVFGICLGHQLVALAIGAKTIKMRFGHHGANHPVL
ECCCCCHHHCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCEEEEEEECCCCCCCCEE
DVDSNRVMITSQNHGFAVATDTLPANARVTHHSLFDGSLQGFELTDHPVFCFQGHPEASP
EECCCEEEEEECCCCEEEEECCCCCCCEEEEEECCCCCCCCCEECCCCEEEECCCCCCCC
GPQDVDYLFSRFVSRMAENKQPA
CCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNRPRAVLVLADGTAFHGDSIGAEGVATGEVAFNTAMTGYQEILTDPSYCRQIVTLTYP
CCCCCEEEEEECCCEECCCCCCCCCEEECCEEHHHHHHHHHHHHCCHHHHHHHHHEECC
HIGNTGVNDEDIESTIDSKVIHVAGLVIRDLPLIASSWRSRQLLPDFLKQHNVPAIAEID
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECC
TRKLTRILRIKGAQAGCIMAGHIDEIEALQQARTFPGLTGMDLARVVSCNQSWEFSEGEW
HHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCC
DPKTGYQTVSERQYRVVVLDFGVKRTILRKLVERGCHVIVYPAQTRIEEILAAKPDGVVL
CCCCCCHHHCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEE
SNGPGDPEACGYAIATTRALLESGVPVFGICLGHQLVALAIGAKTIKMRFGHHGANHPVL
ECCCCCHHHCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCEEEEEEECCCCCCCCEE
DVDSNRVMITSQNHGFAVATDTLPANARVTHHSLFDGSLQGFELTDHPVFCFQGHPEASP
EECCCEEEEEECCCCEEEEECCCCCCCEEEEEECCCCCCCCCEECCCCEEEECCCCCCCC
GPQDVDYLFSRFVSRMAENKQPA
CCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]