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The map label for this gene is topB [H]

Identifier: 114330417

GI number: 114330417

Start: 401630

End: 404128

Strand: Direct

Name: topB [H]

Synonym: Neut_0396

Alternate gene names: 114330417

Gene position: 401630-404128 (Clockwise)

Preceding gene: 114330416

Following gene: 114330419

Centisome position: 15.09

GC content: 46.98

Gene sequence:

>2499_bases
ATGAGTAAAAAACTAATCATCGCCGAGAAACCTTCTGTTGCTAATGATATAGCGCGTGTCCTGGGCGGATTTACCAAGCA
GGGTGACTATTTCGAGAGTGATGAATATGTCTTGTCCTCCGCAATCGGGCATTTGCTGGAATTAATTGTCCCGGAGGAAT
ATGAGGTTAAGCGAGGGAAATGGAGTTTTGATCATTTACCCGTTATTCCTCCACGCTTTGATCTTGCTCCGATCGAGAAA
ACCATGGATCGCCTCAAGCTGTTGAGCAAGCTGATCAAGCGCAAAGATGTTGATGTGTTAATTAATGCATGCGATGCAGG
ACGTGAAGGTGAACTGATTTTTCGTTATATCGTGCGTCATACTGGCAGCAAAAAACCGATTAAACGTTTGTGGTTGCAAT
CTATGACACCCGTGGCCATACGGGAAGCTTTTACAAATCTGCTGGAAGATAGCGAAGTGCAGCCTTTGGCAGATGCGGCC
GTTAGCCGTTCTGAAGCGGATTGGCTAGTAGGTATTAATGGCACGCGTGTCATGACTGCATTCAACTCGCAGGAAGGCGG
ATTTCATAAAACGACAGTCGGGCGTGTGCAAACGCCTACACTGGCCATACTGGTTGAACGAGAAGAGGCGATCAAGAAGT
TTGTGGCCAGGGACTATTGGGAGATTCATGCTGTTTTTCAGGCAGAAAATGGTTCCTATAAAGCAAAATGGTTTGATGAA
AGCTTTAGCAAGCGTAAGGATGAATCTGAATCCAGGGCGGATCGGATTTGGGATCATGCCAGAGCTGAAGCGATTCGGGA
TAAATGCGATGGGCGAACCGGGATTGTTTCCGAAGAAAGCAAACCGACCAGGGAGAACTGTCCTTTACTATACGATCTGA
CCAGTCTGCAACGGGAGGCCAATAGCCGTTTTGGTTTTTCTGCCAAAACGACGTTAGGACTTGCTCAGGCTTTGTATGAG
AAACATAAGGTGCTAACTTATCCTCGTACTGATTCGCGTGCACTTCCTGAAGATTATTCGGAAACAGTCAAGGATACGCT
GCAGGCGCTGAAAGAATCACATTATGATCAGTTTGCCAGTCAGATTTTAAAATCTGACTGGGTAAAACCGAACAAACGGA
TTTTCAATAATGCAAAGGTTTCAGACCACTTCGCAATTATCCCTACAGCGCTGGTTCCAAAAAAACTCAATGAGGCGGAA
GAGAAGCTCTACGACCTGGTGACCAAACGGTTTCTGGCAATTTTCTACCCGGCAGCCGAGTTTTTGGTTACAACCCGCAT
TACTAGGGTGGAAGATGAGCCTTTTAAAACGGAAGGTAAGGTTTTGGTGCATGCTGGCTGGCAGACTGTTTATGGCAAGG
TGGAATCTGTCCGGGGGCAGGAAGAAGAGTCAGTGCTGGTGGCAGTGACACCGGGCGAAACGGTTCTAGCCCAGGAAGTA
ACTGCAGTTGCCAATCAAACGCATCCCCCTGCCAGATATAACGAATCCACGTTGTTATCCGCGATGGAGGGTGCCGGAAA
ACTGGTGGAAGATGAAGAGTTGCGTGCAGCCATGAGTACTAAAGGGTTAGGTACACCCGCTACACGTGCTGCCATCATAG
AAGGCCTGATTCATGAAAATTATGTTGAGCGTTCAGGGAGGGAGTTGCAACCGACAGCCAAAGCATTCTCGTTAGTGACT
TTGTTGCGGGGAATCAAGGTTTCCGAGCTGATTTCACCCGAGTTGACCGGAGACTGGGAATTCAAGTTGCGTCAAATTGA
GCAGGGCCAGCTTAAGCGTGATGTCTTCATGGAGAAGATTGCTACGATGACTCGTCATATCGTCGAGCAAGCTAAAAATC
ATCGAGGTAAGACTATCAGCGGAGATTTTGCAACCCTCCAGGTACCTTGTCCTAAATGCAGTGGCGTAATAAAGGAAACC
TATAAAAAATTTCAATGTCAGCAATGTGATTTTGCATTGTGGAAGATTCTGGCAGGGCGGCAGTTCGAGGTGAATGAAAT
GGAAACGCTGATTTCCACCCGCGAGGTTGGTCCTTTGTCAGGCTTTCGCAGCAAGATGGGACGTGCATTCAACGCCCTTG
TCAGGTTGACAGATGATCATGAAATGAAGTTTGATTTTGGCAACGAAGCCACCCAAACAGAAGAGGAAGTTGATTTTTCG
GCACAGCAAGTGCTTGGAAAATGTCCTCAGTGTGGACATTCTGTATATGAACACAAATTATTTTATGTTTGTGAAAAGGC
GGTAGGGCCAAGCCCTTCCTGCAGTTTCAGAACTGGAAAAATTATTCTGAATCGCGCTATCGATACGGAACAGGTGATCA
AACTGCTTCAGACGGGCAGAACCGATTTGCTGACAGGATTTATTTCCAAGAAAGGGCGGCCTTTTTCTGCTTACCTAGTT
GTAGGGCCGGAAGGCAAGATCGGATTTGAGTTTGAGCAGAAAAAAGTGAAGAGCAAGCGTGCTGACGCTGTTCCGGAAAC
CGGTAAAGCAGCTTCTTGA

Upstream 100 bases:

>100_bases
CCCGTTCATGACCGGCAGCCAGGAGACCGGATGATTTGATCGGCTGACGTAGCCCGTGTGTCAGTTGTTCATTGTGTCTA
TAGCAGTTTATAAGTACCTT

Downstream 100 bases:

>100_bases
TCTGACCGGTAGATACCTTTCGTTTTTTTGTGTTCCCGGAACAGACGTCAGATCAGCCATATATATAAATAATATAGCGA
ACGATGCCTGAAACAGCGGT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 832; Mature: 831

Protein sequence:

>832_residues
MSKKLIIAEKPSVANDIARVLGGFTKQGDYFESDEYVLSSAIGHLLELIVPEEYEVKRGKWSFDHLPVIPPRFDLAPIEK
TMDRLKLLSKLIKRKDVDVLINACDAGREGELIFRYIVRHTGSKKPIKRLWLQSMTPVAIREAFTNLLEDSEVQPLADAA
VSRSEADWLVGINGTRVMTAFNSQEGGFHKTTVGRVQTPTLAILVEREEAIKKFVARDYWEIHAVFQAENGSYKAKWFDE
SFSKRKDESESRADRIWDHARAEAIRDKCDGRTGIVSEESKPTRENCPLLYDLTSLQREANSRFGFSAKTTLGLAQALYE
KHKVLTYPRTDSRALPEDYSETVKDTLQALKESHYDQFASQILKSDWVKPNKRIFNNAKVSDHFAIIPTALVPKKLNEAE
EKLYDLVTKRFLAIFYPAAEFLVTTRITRVEDEPFKTEGKVLVHAGWQTVYGKVESVRGQEEESVLVAVTPGETVLAQEV
TAVANQTHPPARYNESTLLSAMEGAGKLVEDEELRAAMSTKGLGTPATRAAIIEGLIHENYVERSGRELQPTAKAFSLVT
LLRGIKVSELISPELTGDWEFKLRQIEQGQLKRDVFMEKIATMTRHIVEQAKNHRGKTISGDFATLQVPCPKCSGVIKET
YKKFQCQQCDFALWKILAGRQFEVNEMETLISTREVGPLSGFRSKMGRAFNALVRLTDDHEMKFDFGNEATQTEEEVDFS
AQQVLGKCPQCGHSVYEHKLFYVCEKAVGPSPSCSFRTGKIILNRAIDTEQVIKLLQTGRTDLLTGFISKKGRPFSAYLV
VGPEGKIGFEFEQKKVKSKRADAVPETGKAAS

Sequences:

>Translated_832_residues
MSKKLIIAEKPSVANDIARVLGGFTKQGDYFESDEYVLSSAIGHLLELIVPEEYEVKRGKWSFDHLPVIPPRFDLAPIEK
TMDRLKLLSKLIKRKDVDVLINACDAGREGELIFRYIVRHTGSKKPIKRLWLQSMTPVAIREAFTNLLEDSEVQPLADAA
VSRSEADWLVGINGTRVMTAFNSQEGGFHKTTVGRVQTPTLAILVEREEAIKKFVARDYWEIHAVFQAENGSYKAKWFDE
SFSKRKDESESRADRIWDHARAEAIRDKCDGRTGIVSEESKPTRENCPLLYDLTSLQREANSRFGFSAKTTLGLAQALYE
KHKVLTYPRTDSRALPEDYSETVKDTLQALKESHYDQFASQILKSDWVKPNKRIFNNAKVSDHFAIIPTALVPKKLNEAE
EKLYDLVTKRFLAIFYPAAEFLVTTRITRVEDEPFKTEGKVLVHAGWQTVYGKVESVRGQEEESVLVAVTPGETVLAQEV
TAVANQTHPPARYNESTLLSAMEGAGKLVEDEELRAAMSTKGLGTPATRAAIIEGLIHENYVERSGRELQPTAKAFSLVT
LLRGIKVSELISPELTGDWEFKLRQIEQGQLKRDVFMEKIATMTRHIVEQAKNHRGKTISGDFATLQVPCPKCSGVIKET
YKKFQCQQCDFALWKILAGRQFEVNEMETLISTREVGPLSGFRSKMGRAFNALVRLTDDHEMKFDFGNEATQTEEEVDFS
AQQVLGKCPQCGHSVYEHKLFYVCEKAVGPSPSCSFRTGKIILNRAIDTEQVIKLLQTGRTDLLTGFISKKGRPFSAYLV
VGPEGKIGFEFEQKKVKSKRADAVPETGKAAS
>Mature_831_residues
SKKLIIAEKPSVANDIARVLGGFTKQGDYFESDEYVLSSAIGHLLELIVPEEYEVKRGKWSFDHLPVIPPRFDLAPIEKT
MDRLKLLSKLIKRKDVDVLINACDAGREGELIFRYIVRHTGSKKPIKRLWLQSMTPVAIREAFTNLLEDSEVQPLADAAV
SRSEADWLVGINGTRVMTAFNSQEGGFHKTTVGRVQTPTLAILVEREEAIKKFVARDYWEIHAVFQAENGSYKAKWFDES
FSKRKDESESRADRIWDHARAEAIRDKCDGRTGIVSEESKPTRENCPLLYDLTSLQREANSRFGFSAKTTLGLAQALYEK
HKVLTYPRTDSRALPEDYSETVKDTLQALKESHYDQFASQILKSDWVKPNKRIFNNAKVSDHFAIIPTALVPKKLNEAEE
KLYDLVTKRFLAIFYPAAEFLVTTRITRVEDEPFKTEGKVLVHAGWQTVYGKVESVRGQEEESVLVAVTPGETVLAQEVT
AVANQTHPPARYNESTLLSAMEGAGKLVEDEELRAAMSTKGLGTPATRAAIIEGLIHENYVERSGRELQPTAKAFSLVTL
LRGIKVSELISPELTGDWEFKLRQIEQGQLKRDVFMEKIATMTRHIVEQAKNHRGKTISGDFATLQVPCPKCSGVIKETY
KKFQCQQCDFALWKILAGRQFEVNEMETLISTREVGPLSGFRSKMGRAFNALVRLTDDHEMKFDFGNEATQTEEEVDFSA
QQVLGKCPQCGHSVYEHKLFYVCEKAVGPSPSCSFRTGKIILNRAIDTEQVIKLLQTGRTDLLTGFISKKGRPFSAYLVV
GPEGKIGFEFEQKKVKSKRADAVPETGKAAS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=715, Percent_Identity=25.034965034965, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI10835218, Length=624, Percent_Identity=25.8012820512821, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1788061, Length=621, Percent_Identity=32.8502415458937, Blast_Score=319, Evalue=4e-88,
Organism=Escherichia coli, GI1787529, Length=678, Percent_Identity=25.0737463126844, Blast_Score=140, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=694, Percent_Identity=25.5043227665706, Blast_Score=126, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=649, Percent_Identity=24.653312788906, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=703, Percent_Identity=24.8933143669986, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24640096, Length=650, Percent_Identity=24.1538461538462, Blast_Score=117, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24585251, Length=635, Percent_Identity=24.4094488188976, Blast_Score=104, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 93581; Mature: 93450

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLIIAEKPSVANDIARVLGGFTKQGDYFESDEYVLSSAIGHLLELIVPEEYEVKRGK
CCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCC
WSFDHLPVIPPRFDLAPIEKTMDRLKLLSKLIKRKDVDVLINACDAGREGELIFRYIVRH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCHHHHHHHHHH
TGSKKPIKRLWLQSMTPVAIREAFTNLLEDSEVQPLADAAVSRSEADWLVGINGTRVMTA
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECCCCEEEEE
FNSQEGGFHKTTVGRVQTPTLAILVEREEAIKKFVARDYWEIHAVFQAENGSYKAKWFDE
ECCCCCCCEECCCCCCCCCEEEEEEEHHHHHHHHHHHHHHEEEEEEEECCCCEEEEECHH
SFSKRKDESESRADRIWDHARAEAIRDKCDGRTGIVSEESKPTRENCPLLYDLTSLQREA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHH
NSRFGFSAKTTLGLAQALYEKHKVLTYPRTDSRALPEDYSETVKDTLQALKESHYDQFAS
CCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QILKSDWVKPNKRIFNNAKVSDHFAIIPTALVPKKLNEAEEKLYDLVTKRFLAIFYPAAE
HHHHHHCCCCCHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLVTTRITRVEDEPFKTEGKVLVHAGWQTVYGKVESVRGQEEESVLVAVTPGETVLAQEV
HHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHH
TAVANQTHPPARYNESTLLSAMEGAGKLVEDEELRAAMSTKGLGTPATRAAIIEGLIHEN
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YVERSGRELQPTAKAFSLVTLLRGIKVSELISPELTGDWEFKLRQIEQGQLKRDVFMEKI
HHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ATMTRHIVEQAKNHRGKTISGDFATLQVPCPKCSGVIKETYKKFQCQQCDFALWKILAGR
HHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
QFEVNEMETLISTREVGPLSGFRSKMGRAFNALVRLTDDHEMKFDFGNEATQTEEEVDFS
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCHHHHHHH
AQQVLGKCPQCGHSVYEHKLFYVCEKAVGPSPSCSFRTGKIILNRAIDTEQVIKLLQTGR
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCC
TDLLTGFISKKGRPFSAYLVVGPEGKIGFEFEQKKVKSKRADAVPETGKAAS
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SKKLIIAEKPSVANDIARVLGGFTKQGDYFESDEYVLSSAIGHLLELIVPEEYEVKRGK
CCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCC
WSFDHLPVIPPRFDLAPIEKTMDRLKLLSKLIKRKDVDVLINACDAGREGELIFRYIVRH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCHHHHHHHHHH
TGSKKPIKRLWLQSMTPVAIREAFTNLLEDSEVQPLADAAVSRSEADWLVGINGTRVMTA
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECCCCEEEEE
FNSQEGGFHKTTVGRVQTPTLAILVEREEAIKKFVARDYWEIHAVFQAENGSYKAKWFDE
ECCCCCCCEECCCCCCCCCEEEEEEEHHHHHHHHHHHHHHEEEEEEEECCCCEEEEECHH
SFSKRKDESESRADRIWDHARAEAIRDKCDGRTGIVSEESKPTRENCPLLYDLTSLQREA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHH
NSRFGFSAKTTLGLAQALYEKHKVLTYPRTDSRALPEDYSETVKDTLQALKESHYDQFAS
CCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QILKSDWVKPNKRIFNNAKVSDHFAIIPTALVPKKLNEAEEKLYDLVTKRFLAIFYPAAE
HHHHHHCCCCCHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLVTTRITRVEDEPFKTEGKVLVHAGWQTVYGKVESVRGQEEESVLVAVTPGETVLAQEV
HHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHH
TAVANQTHPPARYNESTLLSAMEGAGKLVEDEELRAAMSTKGLGTPATRAAIIEGLIHEN
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YVERSGRELQPTAKAFSLVTLLRGIKVSELISPELTGDWEFKLRQIEQGQLKRDVFMEKI
HHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ATMTRHIVEQAKNHRGKTISGDFATLQVPCPKCSGVIKETYKKFQCQQCDFALWKILAGR
HHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
QFEVNEMETLISTREVGPLSGFRSKMGRAFNALVRLTDDHEMKFDFGNEATQTEEEVDFS
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCHHHHHHH
AQQVLGKCPQCGHSVYEHKLFYVCEKAVGPSPSCSFRTGKIILNRAIDTEQVIKLLQTGR
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCC
TDLLTGFISKKGRPFSAYLVVGPEGKIGFEFEQKKVKSKRADAVPETGKAAS
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA