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The map label for this gene is rph

Identifier: 114330335

GI number: 114330335

Start: 315270

End: 315998

Strand: Direct

Name: rph

Synonym: Neut_0308

Alternate gene names: 114330335

Gene position: 315270-315998 (Clockwise)

Preceding gene: 114330334

Following gene: 114330336

Centisome position: 11.85

GC content: 48.29

Gene sequence:

>729_bases
ATGCCACGATACAATCATCGTACGCTTGCACAAATGCGCCCGATCAGAATAACTCGCCACTATATCCGGCATGCTGAGGG
TTCGGTGTTGATTGAATACGGTGAAACCAAGGTCATTTGTACCGCTAGTGTTATTGAAAAAGTCCCGCCTTTTCTCAAGG
GGGCCGGGCAAGGCTGGTTGACGGCAGAATATGGCATGTTGCCGCGTTCCACAGGTGAAAGAATGCAGCGTGAAGCGGCC
AAAGGTAAACAATCCGGACGTACTATGGAAATTCAGCGCTTGATCGGGAGAGCATTGCGTTCCGTACTGGATCTGAAAAA
ACTTGGTGAACGCACAATTCAGATTGACTGTGATGTGATCCAGGCCGATGGGGGAACTCGTACCGCTAGCATTACCGGCG
CATTTGTTGCCTTGTGTGATGCAGTTGGATATTTGCGTGCAGAAAAAATAATTTCAGAAAATCCGATCAAGGATCACGTT
GCTGCTGTTTCGGTTGGCATACTGAAAGGCCAACCCCTACTGGATCTGGATTATGCGGAAGATTCCAGTTGCGATACTGA
TCTGAATGTCGTCATGACTGGTAATCTGGGATTAGTGGAAGTTCAAGGTACTGCAGAAAAAGTCGTTTTCAGCCGTCAGG
AACTGGATGTCATGCTTGATATGGCTCAACAGGGGCTGCAAGAGCTGTTTGACCTGCAACGTAAAACACTTGAAACCGCT
GCTTCCTGA

Upstream 100 bases:

>100_bases
CGGGATCTCCCGCCAAACCTGACTACCATTTCTCAGTCTGATGACATTATCCGATAGCACGCTGGAATTACATTATCAGT
GCAAAAAATAAGTGATTGAT

Downstream 100 bases:

>100_bases
CAATAAAAGTAACTACACATGCAGCACTCCCTGAGCAGGATTGTTATCGCCAGCAATAATGCCGGAAAGCTTGCTGAAAT
AGGAAAACTGCTGGCTCCCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MPRYNHRTLAQMRPIRITRHYIRHAEGSVLIEYGETKVICTASVIEKVPPFLKGAGQGWLTAEYGMLPRSTGERMQREAA
KGKQSGRTMEIQRLIGRALRSVLDLKKLGERTIQIDCDVIQADGGTRTASITGAFVALCDAVGYLRAEKIISENPIKDHV
AAVSVGILKGQPLLDLDYAEDSSCDTDLNVVMTGNLGLVEVQGTAEKVVFSRQELDVMLDMAQQGLQELFDLQRKTLETA
AS

Sequences:

>Translated_242_residues
MPRYNHRTLAQMRPIRITRHYIRHAEGSVLIEYGETKVICTASVIEKVPPFLKGAGQGWLTAEYGMLPRSTGERMQREAA
KGKQSGRTMEIQRLIGRALRSVLDLKKLGERTIQIDCDVIQADGGTRTASITGAFVALCDAVGYLRAEKIISENPIKDHV
AAVSVGILKGQPLLDLDYAEDSSCDTDLNVVMTGNLGLVEVQGTAEKVVFSRQELDVMLDMAQQGLQELFDLQRKTLETA
AS
>Mature_241_residues
PRYNHRTLAQMRPIRITRHYIRHAEGSVLIEYGETKVICTASVIEKVPPFLKGAGQGWLTAEYGMLPRSTGERMQREAAK
GKQSGRTMEIQRLIGRALRSVLDLKKLGERTIQIDCDVIQADGGTRTASITGAFVALCDAVGYLRAEKIISENPIKDHVA
AVSVGILKGQPLLDLDYAEDSSCDTDLNVVMTGNLGLVEVQGTAEKVVFSRQELDVMLDMAQQGLQELFDLQRKTLETAA
S

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=205, Percent_Identity=64.8780487804878, Blast_Score=270, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI71981632, Length=180, Percent_Identity=28.8888888888889, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_NITEC (Q0AJ79)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746557.1
- ProteinModelPortal:   Q0AJ79
- SMR:   Q0AJ79
- STRING:   Q0AJ79
- GeneID:   4273384
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0308
- NMPDR:   fig|335283.3.peg.2320
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q0AJ79
- ProtClustDB:   PRK00173
- BioCyc:   NEUT335283:NEUT_0308-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26646; Mature: 26515

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYNHRTLAQMRPIRITRHYIRHAEGSVLIEYGETKVICTASVIEKVPPFLKGAGQGWL
CCCCCCCHHHHHCCHHHHHHHHHCCCCCEEEEECCEEEEEEHHHHHHCCHHHHCCCCCCE
TAEYGMLPRSTGERMQREAAKGKQSGRTMEIQRLIGRALRSVLDLKKLGERTIQIDCDVI
EECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEE
QADGGTRTASITGAFVALCDAVGYLRAEKIISENPIKDHVAAVSVGILKGQPLLDLDYAE
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEECCCC
DSSCDTDLNVVMTGNLGLVEVQGTAEKVVFSRQELDVMLDMAQQGLQELFDLQRKTLETA
CCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AS
CC
>Mature Secondary Structure 
PRYNHRTLAQMRPIRITRHYIRHAEGSVLIEYGETKVICTASVIEKVPPFLKGAGQGWL
CCCCCCHHHHHCCHHHHHHHHHCCCCCEEEEECCEEEEEEHHHHHHCCHHHHCCCCCCE
TAEYGMLPRSTGERMQREAAKGKQSGRTMEIQRLIGRALRSVLDLKKLGERTIQIDCDVI
EECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEE
QADGGTRTASITGAFVALCDAVGYLRAEKIISENPIKDHVAAVSVGILKGQPLLDLDYAE
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEECCCC
DSSCDTDLNVVMTGNLGLVEVQGTAEKVVFSRQELDVMLDMAQQGLQELFDLQRKTLETA
CCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA