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The map label for this gene is capD [H]

Identifier: 114330215

GI number: 114330215

Start: 185958

End: 187940

Strand: Direct

Name: capD [H]

Synonym: Neut_0186

Alternate gene names: 114330215

Gene position: 185958-187940 (Clockwise)

Preceding gene: 114330214

Following gene: 114330217

Centisome position: 6.99

GC content: 50.03

Gene sequence:

>1983_bases
ATGGTTAAAAATTACTTCAGACAGTTTGCACCCTCAGTGTTGACCTTGTCCCGTACGATGAAGCGGCTAATTGCTCTTTC
TGTAGATTTCAGTTTGTGTGTTTTGACAGTCTGGCTGGCTTATTACCTGCGTTTGGGTGAGTTTGTCAGTTTTACCAGGC
AGGGTCCATGGGCAGAGGGTGCCATTTGGGCAGCGGTAACCTCCGTTGTACTGGCGCTGCCTATTTTTATTGTTACTGGT
CTTTATCGTGCCATTTTCAGATACAGCGGTTGGCCGGCGTTATTGACGGTAGCACGTGCTATCAGTATTTATGGGCTGAT
CTATGCCACCATCTTTATGGTTATCGGGCTGTCAGGTGTTCCACGCACAATCGGTATCATTCAGCCAATTCTGCTACTCC
TGTTTGTCGGTGCATCGCGTGCGCTGGCACGTGTGTGGCTGGGAGATCAGTACCAGAATATTTTGAGACATATTTCCCGG
CCCCGGGCATTTATTTATGGTGTTGGCCGAACAGGCCGTCAGTTGGCAGTAGCCATGGCCAATAGTCCGGAAATACAGAT
AGCGGGGTTTCTGGATGATGATGATCGCTTGCATGGACATGTACTCAATGGTTTGCCTATCTATAACCCGGGTGATCTGA
CGGCCTTGGTTTCCACCTTGAATATCAGCGATGTCTTGTTGGCAATGCCGGGTATTTCACGCCGTCGTCGTAATGAAATT
TTATGTCAGATTCGTGATGCGCGTGTTTCGGTACGGACATTGCCCAGTGTGACTGATCTGATCCAGGGTAAGGTGGGTAT
TACAGATTTGCGTGAACTGGATATTGATGATCTGCTCGGGCGTGAACCGGTAACACCGAATCATATTCTGTTAGCGAAGA
ATATTCTGGGTAAAGTGGTGTTGGTGACGGGAGCGGGTGGATCGATTGGTAGTGAACTTTGCCGCCAGATTCTTGTTGTG
GGGCCGATCAGGTTGCTGTTGGTTGAACAGAATGAATTTGCGCTTTATATGATTCACCAGGAACTGGAGGGGAAGCTTGC
CAGCCGTGATATTGTGTTGGTGCCTTTATTGGCATCAGTACAGGATGAGGGCCGTATGCGGGAGATCATATCTACTTGGC
ACCCGGATACTGTCTATCATACCGCTGCCTATAAGCATGTGCCGCTGGTGGAGCATAATCCGGCGGAAGGCATTAAGAAC
AATGTGTTTGGTACGCTCCATCTGGCACGGGCCGCCGAGGAAAATGGTGTGATGGATTTTGTGCTGATCAGCACAGACAA
GGCAGTGCGACCTACCAATATCATGGGTGCGAGCAAGCGCCTGGCAGAACTGGTATTACAGGCTCTGGCCGATAACAATT
CCACTACCCGATTTTCCATGGTGCGTTTCGGTAATGTGCTGGGTTCATCGGGCTCAGTGGTGCCGAAATTCCGTCAGCAA
ATCCGGGAGGGAGGTCCGATTACGCTGACTCATCCGGAGATTACCCGCTATTTCATGACAATTCCGGAAGCGGCTCAATT
GGTCATCCAGGCAGGGGCAATGGCCAGGGGCGGGGGGGTATTTGTGCTGGATATGGGAGAATCCGTGAAGATTATGGATC
TGGCACGCCACATGATTGAGCTTTCTGGTTTGACTGTCAGAGATGAGCAGAATCTGGAAGGTGACATTGAAATTGAGATT
ACCGGTTTGCGCCCCGGTGAAAAGCTTTATGAGGAGCTGTTGATTGGAAACAACCCGGAACTGACCTCCCATCCGAGGAT
TATGAGAGCGCATGAAGATTCTGTTCCTTGGGCAGAGCTGGAAGGTAAGCTGAGTGCTTTGGAGATGGCTTTAAATGTGA
ATGATGTCGGTGTCATCCGGTTGATGATGGCACAACTGGTTCCTGGTTATATCCCCAATCAGGAGATCGTAGATTGGGTG
TATCTTGCGCAGGAAGACGAAGCACGGGCAATGGAGTTGGGCAGCAAGGGGACTTCCGGATAA

Upstream 100 bases:

>100_bases
GACGTTGTCGGTAGTGAATTATTTTAAGTACATTGTGCTGACAGCGACAGGTCGTGGTGCAGGGGATCGTATAAGAGAAA
AATCCGGTTGATTAAGTTTT

Downstream 100 bases:

>100_bases
AAGTAAGTAACTCGCTGTTCATTTGTGTATTCCGCAGCGTGCGTTATAAATCACGCCGTTGCAGGCAACGTAGTCGCGCA
GGAGTGTGCAGGTGGTATCA

Product: polysaccharide biosynthesis protein CapD

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MVKNYFRQFAPSVLTLSRTMKRLIALSVDFSLCVLTVWLAYYLRLGEFVSFTRQGPWAEGAIWAAVTSVVLALPIFIVTG
LYRAIFRYSGWPALLTVARAISIYGLIYATIFMVIGLSGVPRTIGIIQPILLLLFVGASRALARVWLGDQYQNILRHISR
PRAFIYGVGRTGRQLAVAMANSPEIQIAGFLDDDDRLHGHVLNGLPIYNPGDLTALVSTLNISDVLLAMPGISRRRRNEI
LCQIRDARVSVRTLPSVTDLIQGKVGITDLRELDIDDLLGREPVTPNHILLAKNILGKVVLVTGAGGSIGSELCRQILVV
GPIRLLLVEQNEFALYMIHQELEGKLASRDIVLVPLLASVQDEGRMREIISTWHPDTVYHTAAYKHVPLVEHNPAEGIKN
NVFGTLHLARAAEENGVMDFVLISTDKAVRPTNIMGASKRLAELVLQALADNNSTTRFSMVRFGNVLGSSGSVVPKFRQQ
IREGGPITLTHPEITRYFMTIPEAAQLVIQAGAMARGGGVFVLDMGESVKIMDLARHMIELSGLTVRDEQNLEGDIEIEI
TGLRPGEKLYEELLIGNNPELTSHPRIMRAHEDSVPWAELEGKLSALEMALNVNDVGVIRLMMAQLVPGYIPNQEIVDWV
YLAQEDEARAMELGSKGTSG

Sequences:

>Translated_660_residues
MVKNYFRQFAPSVLTLSRTMKRLIALSVDFSLCVLTVWLAYYLRLGEFVSFTRQGPWAEGAIWAAVTSVVLALPIFIVTG
LYRAIFRYSGWPALLTVARAISIYGLIYATIFMVIGLSGVPRTIGIIQPILLLLFVGASRALARVWLGDQYQNILRHISR
PRAFIYGVGRTGRQLAVAMANSPEIQIAGFLDDDDRLHGHVLNGLPIYNPGDLTALVSTLNISDVLLAMPGISRRRRNEI
LCQIRDARVSVRTLPSVTDLIQGKVGITDLRELDIDDLLGREPVTPNHILLAKNILGKVVLVTGAGGSIGSELCRQILVV
GPIRLLLVEQNEFALYMIHQELEGKLASRDIVLVPLLASVQDEGRMREIISTWHPDTVYHTAAYKHVPLVEHNPAEGIKN
NVFGTLHLARAAEENGVMDFVLISTDKAVRPTNIMGASKRLAELVLQALADNNSTTRFSMVRFGNVLGSSGSVVPKFRQQ
IREGGPITLTHPEITRYFMTIPEAAQLVIQAGAMARGGGVFVLDMGESVKIMDLARHMIELSGLTVRDEQNLEGDIEIEI
TGLRPGEKLYEELLIGNNPELTSHPRIMRAHEDSVPWAELEGKLSALEMALNVNDVGVIRLMMAQLVPGYIPNQEIVDWV
YLAQEDEARAMELGSKGTSG
>Mature_660_residues
MVKNYFRQFAPSVLTLSRTMKRLIALSVDFSLCVLTVWLAYYLRLGEFVSFTRQGPWAEGAIWAAVTSVVLALPIFIVTG
LYRAIFRYSGWPALLTVARAISIYGLIYATIFMVIGLSGVPRTIGIIQPILLLLFVGASRALARVWLGDQYQNILRHISR
PRAFIYGVGRTGRQLAVAMANSPEIQIAGFLDDDDRLHGHVLNGLPIYNPGDLTALVSTLNISDVLLAMPGISRRRRNEI
LCQIRDARVSVRTLPSVTDLIQGKVGITDLRELDIDDLLGREPVTPNHILLAKNILGKVVLVTGAGGSIGSELCRQILVV
GPIRLLLVEQNEFALYMIHQELEGKLASRDIVLVPLLASVQDEGRMREIISTWHPDTVYHTAAYKHVPLVEHNPAEGIKN
NVFGTLHLARAAEENGVMDFVLISTDKAVRPTNIMGASKRLAELVLQALADNNSTTRFSMVRFGNVLGSSGSVVPKFRQQ
IREGGPITLTHPEITRYFMTIPEAAQLVIQAGAMARGGGVFVLDMGESVKIMDLARHMIELSGLTVRDEQNLEGDIEIEI
TGLRPGEKLYEELLIGNNPELTSHPRIMRAHEDSVPWAELEGKLSALEMALNVNDVGVIRLMMAQLVPGYIPNQEIVDWV
YLAQEDEARAMELGSKGTSG

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 72738; Mature: 72738

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKNYFRQFAPSVLTLSRTMKRLIALSVDFSLCVLTVWLAYYLRLGEFVSFTRQGPWAEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AIWAAVTSVVLALPIFIVTGLYRAIFRYSGWPALLTVARAISIYGLIYATIFMVIGLSGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRTIGIIQPILLLLFVGASRALARVWLGDQYQNILRHISRPRAFIYGVGRTGRQLAVAMA
CHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEC
NSPEIQIAGFLDDDDRLHGHVLNGLPIYNPGDLTALVSTLNISDVLLAMPGISRRRRNEI
CCCCEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHH
LCQIRDARVSVRTLPSVTDLIQGKVGITDLRELDIDDLLGREPVTPNHILLAKNILGKVV
HEEECCCEEEEHHCCHHHHHHCCCCCCCHHHHCCHHHHCCCCCCCCCEEEEEHHHHCEEE
LVTGAGGSIGSELCRQILVVGPIRLLLVEQNEFALYMIHQELEGKLASRDIVLVPLLASV
EEECCCCCHHHHHHHHHHHHCCEEEEEEECCCEEEEEEEHHHCCHHCCCCEEEEEHHHHH
QDEGRMREIISTWHPDTVYHTAAYKHVPLVEHNPAEGIKNNVFGTLHLARAAEENGVMDF
CCCHHHHHHHHHCCCCCEEECHHHCCCCCCCCCCCCCHHHCCCEEEHHHHHHCCCCCEEE
VLISTDKAVRPTNIMGASKRLAELVLQALADNNSTTRFSMVRFGNVLGSSGSVVPKFRQQ
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCHHHHHH
IREGGPITLTHPEITRYFMTIPEAAQLVIQAGAMARGGGVFVLDMGESVKIMDLARHMIE
HHCCCCEEEECHHHHHHHHCCCHHHHHHHHHCCEECCCCEEEEECCCCCHHHHHHHHHHH
LSGLTVRDEQNLEGDIEIEITGLRPGEKLYEELLIGNNPELTSHPRIMRAHEDSVPWAEL
HCCCEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHH
EGKLSALEMALNVNDVGVIRLMMAQLVPGYIPNQEIVDWVYLAQEDEARAMELGSKGTSG
CCCHHHHHHEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHEEECCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MVKNYFRQFAPSVLTLSRTMKRLIALSVDFSLCVLTVWLAYYLRLGEFVSFTRQGPWAEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AIWAAVTSVVLALPIFIVTGLYRAIFRYSGWPALLTVARAISIYGLIYATIFMVIGLSGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRTIGIIQPILLLLFVGASRALARVWLGDQYQNILRHISRPRAFIYGVGRTGRQLAVAMA
CHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEC
NSPEIQIAGFLDDDDRLHGHVLNGLPIYNPGDLTALVSTLNISDVLLAMPGISRRRRNEI
CCCCEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHH
LCQIRDARVSVRTLPSVTDLIQGKVGITDLRELDIDDLLGREPVTPNHILLAKNILGKVV
HEEECCCEEEEHHCCHHHHHHCCCCCCCHHHHCCHHHHCCCCCCCCCEEEEEHHHHCEEE
LVTGAGGSIGSELCRQILVVGPIRLLLVEQNEFALYMIHQELEGKLASRDIVLVPLLASV
EEECCCCCHHHHHHHHHHHHCCEEEEEEECCCEEEEEEEHHHCCHHCCCCEEEEEHHHHH
QDEGRMREIISTWHPDTVYHTAAYKHVPLVEHNPAEGIKNNVFGTLHLARAAEENGVMDF
CCCHHHHHHHHHCCCCCEEECHHHCCCCCCCCCCCCCHHHCCCEEEHHHHHHCCCCCEEE
VLISTDKAVRPTNIMGASKRLAELVLQALADNNSTTRFSMVRFGNVLGSSGSVVPKFRQQ
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCHHHHHH
IREGGPITLTHPEITRYFMTIPEAAQLVIQAGAMARGGGVFVLDMGESVKIMDLARHMIE
HHCCCCEEEECHHHHHHHHCCCHHHHHHHHHCCEECCCCEEEEECCCCCHHHHHHHHHHH
LSGLTVRDEQNLEGDIEIEITGLRPGEKLYEELLIGNNPELTSHPRIMRAHEDSVPWAEL
HCCCEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHH
EGKLSALEMALNVNDVGVIRLMMAQLVPGYIPNQEIVDWVYLAQEDEARAMELGSKGTSG
CCCHHHHHHEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHEEECCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]