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The map label for this gene is yfhA [H]

Identifier: 114330169

GI number: 114330169

Start: 136755

End: 138122

Strand: Direct

Name: yfhA [H]

Synonym: Neut_0138

Alternate gene names: 114330169

Gene position: 136755-138122 (Clockwise)

Preceding gene: 114330168

Following gene: 114330172

Centisome position: 5.14

GC content: 50.07

Gene sequence:

>1368_bases
ATGATGCAAAGTAAAAAAATTCTTCTGGTGGATGATGATCCGGATTTACTTGAGCTGCTTTCCATTCGCCTGACAGCAGC
CGGATATGAAACGGTTCCCGCCGAAAGTGCCGAAGCGGCCATCAATTATCTCGACATATCACGACCGCATCTCGTCATTA
GTGACATACAGATGAGCGGCATGGATGGTATGGCATTGTTCGAGCATATCCATCGTCATATTCCAACGCTTCCGGTCATT
ATCCTGACCGCGTTCGGTACCATTCCGGATGCTGTCGCGGCAACGCAGCGCGGGATTTTTGGTTATCTCACAAAGCCATA
TAATCCTAAAATTCTGCTGAGCCAGGTCGAGCGTGCAATTGATCTGGCGCCTGCTGTAGATACAATCTCCAGCAAAGTGC
CTGCCCCGACATGGCGGAAAGCGATCATTACTCGCAGTGCGCTCATGGAAGATCTGCTTGCCAAAGTGGATCGCGTCGCG
CAAGGCAATGCCAGTGTGCTGCTGTCTGGTGAAAGCGGTGTCGGCAAGGAATTATTTGCTCGTGCCATTCATCAAGCATC
CAAACGCTGTGAGCAGCCGTTCATCACCATTAATTGTGCGGCCATTCCTGAACAACTCCTGGAGTCTGAGCTGTTCGGTC
ATGCCAAAGGTGCATTTACCGGGGCAGTCCGGGAGCATAAAGGGCTGTTCCAGCTGGCAGAAGGAGGTAGTTTGTTTCTG
GACGAGATTGGTGATATGCCTTTGCTGTTGCAGGCAAAATTGCTACACGCACTGCAGGAGAGGGTTATCCGGCCGGTTGG
AACAGCTCAGAGCATCCCGATTGATGTACGTATCATTTCTGCTACGCATAAAGATCTGAAATCGGAGATCCAGGCAGGAA
ATTTCCGTGAAGACCTGTATTACCGGCTTTTTGTGGTGGGTTTGACAATTCCTTCACTTGCCCAGCGTGGTGAAGACATT
CCTCTGCTGGCCAATCATTTTCTGCGTGTATTTGCCGAGAAGCACCAAAAGGATATCAATGGCTTTTCTCCGGAAGCAAT
CAGTTTTCTGCTGGCATCTTCCTGGCCAGGCAATATCCGCCAGCTGATGAATGTTATCGAACAAAGTATCGTCATGAGTG
CGGTTCCTCTCATCTCCAGCGAACTGATCCGCGATGCCATGCACAAGGATGAAGAGCAGATGATCTCGTTTGAGGAAGCC
CGCAAACAGTTTGAAAGAGATTATCTCGTCAAGGTGCTGAAAATAACTGCCGGAAATGTAACCCAGGCGGCCAGGCTGGC
CAAGCGCAACCGCACCGAGTTTTACAAACTGCTGCAACGACACCAGTTGGATTTCACACTCTATAAGTCCTTGCAGGAAA
AGGTCTGA

Upstream 100 bases:

>100_bases
GAGGCAGAAGTAAAAACACTGCAAGATAAGATCAACGCAATTAAAAATATTGAAAAACACTTCATGCGCAAAAGTGCATT
CTGATTAATTAGCTGGCTAA

Downstream 100 bases:

>100_bases
TTTCCCGTTTATTTCACTAAAGGCGGGAATTTCGCAATGATTTCAATGATGACACCTTCCCGCAACACTTTCAGGGGCAA
CCAGGTACCCGGTGCCTGAC

Product: two component, sigma54 specific, transcriptional regulator, Fis family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MMQSKKILLVDDDPDLLELLSIRLTAAGYETVPAESAEAAINYLDISRPHLVISDIQMSGMDGMALFEHIHRHIPTLPVI
ILTAFGTIPDAVAATQRGIFGYLTKPYNPKILLSQVERAIDLAPAVDTISSKVPAPTWRKAIITRSALMEDLLAKVDRVA
QGNASVLLSGESGVGKELFARAIHQASKRCEQPFITINCAAIPEQLLESELFGHAKGAFTGAVREHKGLFQLAEGGSLFL
DEIGDMPLLLQAKLLHALQERVIRPVGTAQSIPIDVRIISATHKDLKSEIQAGNFREDLYYRLFVVGLTIPSLAQRGEDI
PLLANHFLRVFAEKHQKDINGFSPEAISFLLASSWPGNIRQLMNVIEQSIVMSAVPLISSELIRDAMHKDEEQMISFEEA
RKQFERDYLVKVLKITAGNVTQAARLAKRNRTEFYKLLQRHQLDFTLYKSLQEKV

Sequences:

>Translated_455_residues
MMQSKKILLVDDDPDLLELLSIRLTAAGYETVPAESAEAAINYLDISRPHLVISDIQMSGMDGMALFEHIHRHIPTLPVI
ILTAFGTIPDAVAATQRGIFGYLTKPYNPKILLSQVERAIDLAPAVDTISSKVPAPTWRKAIITRSALMEDLLAKVDRVA
QGNASVLLSGESGVGKELFARAIHQASKRCEQPFITINCAAIPEQLLESELFGHAKGAFTGAVREHKGLFQLAEGGSLFL
DEIGDMPLLLQAKLLHALQERVIRPVGTAQSIPIDVRIISATHKDLKSEIQAGNFREDLYYRLFVVGLTIPSLAQRGEDI
PLLANHFLRVFAEKHQKDINGFSPEAISFLLASSWPGNIRQLMNVIEQSIVMSAVPLISSELIRDAMHKDEEQMISFEEA
RKQFERDYLVKVLKITAGNVTQAARLAKRNRTEFYKLLQRHQLDFTLYKSLQEKV
>Mature_455_residues
MMQSKKILLVDDDPDLLELLSIRLTAAGYETVPAESAEAAINYLDISRPHLVISDIQMSGMDGMALFEHIHRHIPTLPVI
ILTAFGTIPDAVAATQRGIFGYLTKPYNPKILLSQVERAIDLAPAVDTISSKVPAPTWRKAIITRSALMEDLLAKVDRVA
QGNASVLLSGESGVGKELFARAIHQASKRCEQPFITINCAAIPEQLLESELFGHAKGAFTGAVREHKGLFQLAEGGSLFL
DEIGDMPLLLQAKLLHALQERVIRPVGTAQSIPIDVRIISATHKDLKSEIQAGNFREDLYYRLFVVGLTIPSLAQRGEDI
PLLANHFLRVFAEKHQKDINGFSPEAISFLLASSWPGNIRQLMNVIEQSIVMSAVPLISSELIRDAMHKDEEQMISFEEA
RKQFERDYLVKVLKITAGNVTQAARLAKRNRTEFYKLLQRHQLDFTLYKSLQEKV

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=57.2072072072072, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1788550, Length=457, Percent_Identity=42.6695842450766, Blast_Score=347, Evalue=9e-97,
Organism=Escherichia coli, GI1790437, Length=443, Percent_Identity=42.2121896162528, Blast_Score=344, Evalue=6e-96,
Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=38.6266094420601, Blast_Score=301, Evalue=8e-83,
Organism=Escherichia coli, GI87082117, Length=316, Percent_Identity=45.253164556962, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1789233, Length=243, Percent_Identity=50.2057613168724, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI1789087, Length=310, Percent_Identity=41.9354838709677, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87082152, Length=313, Percent_Identity=38.0191693290735, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1786524, Length=322, Percent_Identity=37.2670807453416, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI87081872, Length=311, Percent_Identity=36.6559485530547, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1787583, Length=301, Percent_Identity=36.8770764119601, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789828, Length=264, Percent_Identity=33.7121212121212, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87081858, Length=302, Percent_Identity=30.1324503311258, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786599, Length=116, Percent_Identity=31.8965517241379, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789809, Length=145, Percent_Identity=30.3448275862069, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50616; Mature: 50616

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQSKKILLVDDDPDLLELLSIRLTAAGYETVPAESAEAAINYLDISRPHLVISDIQMSG
CCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCHHCC
MDGMALFEHIHRHIPTLPVIILTAFGTIPDAVAATQRGIFGYLTKPYNPKILLSQVERAI
CCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHH
DLAPAVDTISSKVPAPTWRKAIITRSALMEDLLAKVDRVAQGNASVLLSGESGVGKELFA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RAIHQASKRCEQPFITINCAAIPEQLLESELFGHAKGAFTGAVREHKGLFQLAEGGSLFL
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHEECCCCCCHH
DEIGDMPLLLQAKLLHALQERVIRPVGTAQSIPIDVRIISATHKDLKSEIQAGNFREDLY
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHCCCCHHHHH
YRLFVVGLTIPSLAQRGEDIPLLANHFLRVFAEKHQKDINGFSPEAISFLLASSWPGNIR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
QLMNVIEQSIVMSAVPLISSELIRDAMHKDEEQMISFEEARKQFERDYLVKVLKITAGNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TQAARLAKRNRTEFYKLLQRHQLDFTLYKSLQEKV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MMQSKKILLVDDDPDLLELLSIRLTAAGYETVPAESAEAAINYLDISRPHLVISDIQMSG
CCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCHHCC
MDGMALFEHIHRHIPTLPVIILTAFGTIPDAVAATQRGIFGYLTKPYNPKILLSQVERAI
CCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHH
DLAPAVDTISSKVPAPTWRKAIITRSALMEDLLAKVDRVAQGNASVLLSGESGVGKELFA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RAIHQASKRCEQPFITINCAAIPEQLLESELFGHAKGAFTGAVREHKGLFQLAEGGSLFL
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHEECCCCCCHH
DEIGDMPLLLQAKLLHALQERVIRPVGTAQSIPIDVRIISATHKDLKSEIQAGNFREDLY
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHCCCCHHHHH
YRLFVVGLTIPSLAQRGEDIPLLANHFLRVFAEKHQKDINGFSPEAISFLLASSWPGNIR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
QLMNVIEQSIVMSAVPLISSELIRDAMHKDEEQMISFEEARKQFERDYLVKVLKITAGNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TQAARLAKRNRTEFYKLLQRHQLDFTLYKSLQEKV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]