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The map label for this gene is topB [H]

Identifier: 114330147

GI number: 114330147

Start: 112738

End: 114753

Strand: Reverse

Name: topB [H]

Synonym: Neut_0116

Alternate gene names: 114330147

Gene position: 114753-112738 (Counterclockwise)

Preceding gene: 114330148

Following gene: 114330146

Centisome position: 4.31

GC content: 60.62

Gene sequence:

>2016_bases
ATGAGGTTATTTCTGTGCGAGAAGCCTTCCCAGGGCAAAGACATCGGCAAAGTGCTTGGCGCCACGCAGCGCGACAACGG
TTGCCTGAAAGGCCCGGGCGTCATCGTGACCTGGTGCATTGGCCATCTGGTCGAGGCGGCGCCGCCAGAAGCTTATGACG
CAAATCTCAAACACTGGTCCATCGAACAGTTGCCCATTATTCCCGAGCGCTGGCGTATCGAAGTCAAACCCAAGACCGCC
GCGCAATTCAAAGTCGTCAAGCAGTTGCTGGCACAAGCTACCGAGTTGGTGATCGCCACCGACGCGGATCGTGAAGGCGA
AATGATCGCCCGTGAAATTGTTGAGCTGTGTGGCTACCGAGGGCCTATTCAACGCCTGTGGCTATCGGCCCTGAATGATG
CATCGATCCGCAAGGCATTGGCTGCCCTGAAACCGTCATCTGAGACGCTGCCCATGTACCATTCGGCCTTGGCGCGCTCC
CGCGCCGACTGGCTGGTGGGCATGAACCTGAGTCGGCTTTTCACTCTATTGGGTCGGCAGGCTGGCTACGATGGCGTGTT
GTCGGTAGGTCGGGTGCAAACGCCCACGTTAAAGCTCGTGGTGGATCGCGACCGTGAAATCAATCAGTTTGTCTCCATCC
CATTCTGGGCTATCGACGTAGCGCTCTCAGCAACAGGACAATCTTTCATCGCGCAGTGGCTGGCCCCCGAAGCCTGCACC
GACGAGGCGGGAAGGTGCCTGCAGGAGTCCGTGGCACAACAGGCGGCGCAACAATTGCGTGCAAACGGCACCGCTCAGGT
CGCGTCCGTCGAAACCGAACGGGTGCGCGAGGGCCCGCCATTGCCCTTCGATCTTGGGACGTTGCAGGAAGTCTGTTCCA
AGCAGCTTGGCCTGGATGTGCAGGAAACGCTGGACATTGCCCAGGCGTTGTACGAAACGCATAAGGCCACCACCTATCCA
CGCTCGGATTCGGGCTACCTGCCCGAGAGTATGCAGGCCGATGTGCCAGCCGTGCTCGACAGCCTGCTCAGAACCGATCC
CGCCTTGCGGCCCGTGATGGAGCAGCTTGATCGCAGACAGCGTTCACGGGCCTGGAACGACAGCAAGGTCACCGCTCACC
ATGGCATCATCCCCACGCTGGAACCCGCGAACCTCTCGGCCATGAGCGAGAAGGAGCTGGCCGTTTATCGACTGATCCGT
GCCCATTACCTAGCACAGTTTCTACCGCATCATGAATTTGACCGCACCATTGCGCGGCTTTCGTGCGCGGGTCAACATCT
TCAGGCCGTAGGCAAACAGATTGTCATCCCAGGATGGCGGCTGGTGCTGGTCGAGCAGCAAGCGGAGGATGCCGACGATG
AGGCCGCCCCGCGCAGTCAAGTGTTGCCCGCACTGAACCAAGGCCTGTCCTGCCAGATGAACACCGTTGATCTTAAGGCG
CTCAAGACGCTGCCTCCCCGGCCCTACACCCAAGGAGAGCTGGTCAAGGCCATGAAAAGTGTCGCCAAGCTGGTGTCCGA
CCCACGCCTGAAGCAGAAGCTCAAGGACACGGTTGGCATCGGCACCGAGGCCACTCGCGCCAATATCATCAACGGGCTGC
TGACACGTGGCTATCTGATCAAGAAGGGCCGGGCGATCCGTGCGTCGGAAGCGGCTGGCACGCTCATTGATACGGTTCCC
GCGGCCATTGCTGACCCCGGCACGACAGCAGTCTGGGAGCAGGCCCTGGACATGATCGAGGCGGGCGAGCTTACCCATGA
CGTCTTCACCGGCAAGCAGTCGGCCTGGATTTCACAGATCATCGCCCAGTACCAGGGCACAGCCATGACGATCAAGGTGC
CCCAAGGTCCAGCTTGCCCGCAGTGCGGCTCGGCCACACGCCAACGCACTGGCAAAAATGGTCCGTTCTGGTCATGCTCG
CGTTATCCCGAGTGCAAAGGCACCGTGCCGGTTGACTCCGCAACGCCCAAGCGCAGCGCGCCGCGCAAGCGCCGCAGCGC
ATCCAAGACATCCTGA

Upstream 100 bases:

>100_bases
AAAGGCGTTTGCCCCGAGTACGATTTGTTTGCTGTCCGAATCTGACACTGCCGCCATCCTGATCCCATTCGCATTAACAA
CGAGATTCGGATGGAACAGT

Downstream 100 bases:

>100_bases
AATGCTCCAAACCCCTCAGTGCCGTGCAGACCGACTCGGTTGCATGGCATTCGTCCCGTAGCCCTCTCGGGACTCCCAGC
GCGCAAATTCTTCTGCAACC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MRLFLCEKPSQGKDIGKVLGATQRDNGCLKGPGVIVTWCIGHLVEAAPPEAYDANLKHWSIEQLPIIPERWRIEVKPKTA
AQFKVVKQLLAQATELVIATDADREGEMIAREIVELCGYRGPIQRLWLSALNDASIRKALAALKPSSETLPMYHSALARS
RADWLVGMNLSRLFTLLGRQAGYDGVLSVGRVQTPTLKLVVDRDREINQFVSIPFWAIDVALSATGQSFIAQWLAPEACT
DEAGRCLQESVAQQAAQQLRANGTAQVASVETERVREGPPLPFDLGTLQEVCSKQLGLDVQETLDIAQALYETHKATTYP
RSDSGYLPESMQADVPAVLDSLLRTDPALRPVMEQLDRRQRSRAWNDSKVTAHHGIIPTLEPANLSAMSEKELAVYRLIR
AHYLAQFLPHHEFDRTIARLSCAGQHLQAVGKQIVIPGWRLVLVEQQAEDADDEAAPRSQVLPALNQGLSCQMNTVDLKA
LKTLPPRPYTQGELVKAMKSVAKLVSDPRLKQKLKDTVGIGTEATRANIINGLLTRGYLIKKGRAIRASEAAGTLIDTVP
AAIADPGTTAVWEQALDMIEAGELTHDVFTGKQSAWISQIIAQYQGTAMTIKVPQGPACPQCGSATRQRTGKNGPFWSCS
RYPECKGTVPVDSATPKRSAPRKRRSASKTS

Sequences:

>Translated_671_residues
MRLFLCEKPSQGKDIGKVLGATQRDNGCLKGPGVIVTWCIGHLVEAAPPEAYDANLKHWSIEQLPIIPERWRIEVKPKTA
AQFKVVKQLLAQATELVIATDADREGEMIAREIVELCGYRGPIQRLWLSALNDASIRKALAALKPSSETLPMYHSALARS
RADWLVGMNLSRLFTLLGRQAGYDGVLSVGRVQTPTLKLVVDRDREINQFVSIPFWAIDVALSATGQSFIAQWLAPEACT
DEAGRCLQESVAQQAAQQLRANGTAQVASVETERVREGPPLPFDLGTLQEVCSKQLGLDVQETLDIAQALYETHKATTYP
RSDSGYLPESMQADVPAVLDSLLRTDPALRPVMEQLDRRQRSRAWNDSKVTAHHGIIPTLEPANLSAMSEKELAVYRLIR
AHYLAQFLPHHEFDRTIARLSCAGQHLQAVGKQIVIPGWRLVLVEQQAEDADDEAAPRSQVLPALNQGLSCQMNTVDLKA
LKTLPPRPYTQGELVKAMKSVAKLVSDPRLKQKLKDTVGIGTEATRANIINGLLTRGYLIKKGRAIRASEAAGTLIDTVP
AAIADPGTTAVWEQALDMIEAGELTHDVFTGKQSAWISQIIAQYQGTAMTIKVPQGPACPQCGSATRQRTGKNGPFWSCS
RYPECKGTVPVDSATPKRSAPRKRRSASKTS
>Mature_671_residues
MRLFLCEKPSQGKDIGKVLGATQRDNGCLKGPGVIVTWCIGHLVEAAPPEAYDANLKHWSIEQLPIIPERWRIEVKPKTA
AQFKVVKQLLAQATELVIATDADREGEMIAREIVELCGYRGPIQRLWLSALNDASIRKALAALKPSSETLPMYHSALARS
RADWLVGMNLSRLFTLLGRQAGYDGVLSVGRVQTPTLKLVVDRDREINQFVSIPFWAIDVALSATGQSFIAQWLAPEACT
DEAGRCLQESVAQQAAQQLRANGTAQVASVETERVREGPPLPFDLGTLQEVCSKQLGLDVQETLDIAQALYETHKATTYP
RSDSGYLPESMQADVPAVLDSLLRTDPALRPVMEQLDRRQRSRAWNDSKVTAHHGIIPTLEPANLSAMSEKELAVYRLIR
AHYLAQFLPHHEFDRTIARLSCAGQHLQAVGKQIVIPGWRLVLVEQQAEDADDEAAPRSQVLPALNQGLSCQMNTVDLKA
LKTLPPRPYTQGELVKAMKSVAKLVSDPRLKQKLKDTVGIGTEATRANIINGLLTRGYLIKKGRAIRASEAAGTLIDTVP
AAIADPGTTAVWEQALDMIEAGELTHDVFTGKQSAWISQIIAQYQGTAMTIKVPQGPACPQCGSATRQRTGKNGPFWSCS
RYPECKGTVPVDSATPKRSAPRKRRSASKTS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=656, Percent_Identity=23.9329268292683, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI4507635, Length=540, Percent_Identity=22.4074074074074, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI1788061, Length=654, Percent_Identity=38.9908256880734, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1787529, Length=687, Percent_Identity=25.764192139738, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=567, Percent_Identity=24.6913580246914, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=571, Percent_Identity=23.8178633975482, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=498, Percent_Identity=27.3092369477912, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585251, Length=687, Percent_Identity=25.0363901018923, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24640096, Length=463, Percent_Identity=25.0539956803456, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 73493; Mature: 73493

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFLCEKPSQGKDIGKVLGATQRDNGCLKGPGVIVTWCIGHLVEAAPPEAYDANLKHWS
CEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
IEQLPIIPERWRIEVKPKTAAQFKVVKQLLAQATELVIATDADREGEMIAREIVELCGYR
CCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCC
GPIQRLWLSALNDASIRKALAALKPSSETLPMYHSALARSRADWLVGMNLSRLFTLLGRQ
CHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
AGYDGVLSVGRVQTPTLKLVVDRDREINQFVSIPFWAIDVALSATGQSFIAQWLAPEACT
CCCCHHHCCCCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCHHH
DEAGRCLQESVAQQAAQQLRANGTAQVASVETERVREGPPLPFDLGTLQEVCSKQLGLDV
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCH
QETLDIAQALYETHKATTYPRSDSGYLPESMQADVPAVLDSLLRTDPALRPVMEQLDRRQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
RSRAWNDSKVTAHHGIIPTLEPANLSAMSEKELAVYRLIRAHYLAQFLPHHEFDRTIARL
HHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SCAGQHLQAVGKQIVIPGWRLVLVEQQAEDADDEAAPRSQVLPALNQGLSCQMNTVDLKA
HHHHHHHHHHCCEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEECCHHHHH
LKTLPPRPYTQGELVKAMKSVAKLVSDPRLKQKLKDTVGIGTEATRANIINGLLTRGYLI
HHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCE
KKGRAIRASEAAGTLIDTVPAAIADPGTTAVWEQALDMIEAGELTHDVFTGKQSAWISQI
ECCCCEECHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHH
IAQYQGTAMTIKVPQGPACPQCGSATRQRTGKNGPFWSCSRYPECKGTVPVDSATPKRSA
HHHHCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PRKRRSASKTS
CHHHHCCCCCC
>Mature Secondary Structure
MRLFLCEKPSQGKDIGKVLGATQRDNGCLKGPGVIVTWCIGHLVEAAPPEAYDANLKHWS
CEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
IEQLPIIPERWRIEVKPKTAAQFKVVKQLLAQATELVIATDADREGEMIAREIVELCGYR
CCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCC
GPIQRLWLSALNDASIRKALAALKPSSETLPMYHSALARSRADWLVGMNLSRLFTLLGRQ
CHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
AGYDGVLSVGRVQTPTLKLVVDRDREINQFVSIPFWAIDVALSATGQSFIAQWLAPEACT
CCCCHHHCCCCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCHHH
DEAGRCLQESVAQQAAQQLRANGTAQVASVETERVREGPPLPFDLGTLQEVCSKQLGLDV
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCH
QETLDIAQALYETHKATTYPRSDSGYLPESMQADVPAVLDSLLRTDPALRPVMEQLDRRQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
RSRAWNDSKVTAHHGIIPTLEPANLSAMSEKELAVYRLIRAHYLAQFLPHHEFDRTIARL
HHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SCAGQHLQAVGKQIVIPGWRLVLVEQQAEDADDEAAPRSQVLPALNQGLSCQMNTVDLKA
HHHHHHHHHHCCEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEECCHHHHH
LKTLPPRPYTQGELVKAMKSVAKLVSDPRLKQKLKDTVGIGTEATRANIINGLLTRGYLI
HHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCE
KKGRAIRASEAAGTLIDTVPAAIADPGTTAVWEQALDMIEAGELTHDVFTGKQSAWISQI
ECCCCEECHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHH
IAQYQGTAMTIKVPQGPACPQCGSATRQRTGKNGPFWSCSRYPECKGTVPVDSATPKRSA
HHHHCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PRKRRSASKTS
CHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]