Click here to switch to the map view.

The map label for this gene is ngoBVM [H]

Identifier: 114330146

GI number: 114330146

Start: 110695

End: 112188

Strand: Reverse

Name: ngoBVM [H]

Synonym: Neut_0115

Alternate gene names: 114330146

Gene position: 112188-110695 (Counterclockwise)

Preceding gene: 114330147

Following gene: 114330145

Centisome position: 4.22

GC content: 62.25

Gene sequence:

>1494_bases
ATGCACCCTCAGCCTACCCACACTACGCTGCAATACGGCAGCGTCTGCAGTGGCATTGAAGCCGTGAGTCTCGCGTGGCA
ACCACAAGGCCCGCAAGCCGCGTGGTTTTCCGAGATTGAACCGTTCCCGTGCGCCGTGCTCGCCCATCGCTACCCCGACG
TGCCCAACCTGGGCGATATGACACAGATCGCCGAGCAGGTGCGAGCGGGCCTAGTTGTCGCCCCGGACATTCTCGTGGGC
GGTACCCCTTGCCAGACGTTTAGCATCGCCGGTGCACGTCAGGGACTCTCTGACCCGCGTGGCGCCTTAACCCTTAAGTA
TGTGGAGCTTGCCAATGCCATCGACCAAACTCGCCAAAAAAATCAACGTCCGCCCGCCACGATCATCTGGGAAAACGTCC
CAGGTGTCCTTAGCGACCGCTCCAACGCCTTTGGCTGCCTGCTGGGCGCACTGGCCGGAGAAAGCCGCGCGCTCGAGCCG
CCAGGGGCAAAATGGACGCACGCAGGTTACGTGTCTGGCCCCCAGCGCCGCCTCGCCTGGCGCGTGCTCGACGCTCAACA
TTTCGGTGTCGCCCAGCGTCGCAAACGCGTGTTTCTTGTGGCAAGTGGTCGAAATGACGTCGATCCCGCCGAAGTACTTT
TTGAGCGCGACGGCTTGCACGGGGATTCTTCGCAGGGCTTTGCGCCGTGGCAAGACGCTGCCTGTGCTGCTGGAGCAAGC
ACTGCATCACCAGGCGACTATGTCGGACTGAATCAGCATTATGACAAGGTCAGGGTGACGTTCGGCTTTGGTGGTGGCAA
CACCTCGGGGCCGATTGATGTCGCCGCTTGCCTAACAGCTGCCGCAGGCCCTAAGAATGACTTTGAGGTTGAAACTTTTG
TGGTGCAGTCTGTGGCGGGTGACATCAGCCACACGCTGGGCACGTCCACAGGCAGCAGCGAAGACGGCACGGGCAGGGGC
GTACCCATCATCACCACCTATGCGCGGCAAGCCATGGCGTTTGCACAGAACTCTCGCGGCGAGTTGCGCTTGGAGTCCGG
CCACGGGCAGATCGCTGGTGCGCTCTCGACGGGCGGTGGCAAGCCCGGGCAGGGCCTGCCCATGGTGCTTAGCGTGGCAT
TGCGTGGTCGCGACCACGGCATCGCCGCCGAGCTGGGAGGCACCGTCGCACCGGCTCTGCGTGCCAGCAGTGGCGGCGGT
GACAAAAACCACGTGCTGATACCCGACTATGACACCTACTTCCAGTACACCGGAAATAGGCCAAGTGCCAGCGACTGGTC
GCAGTGGCGGGTGCGTCGGCTGATGCCAGTGGAATGCGAGCGCTTGCAGGGCATGCCGGACGACTACACCCAAGTGCCAT
ATCGCGGCAAGCCCGCAACTGATACCCAGCGTTACAAGGCGATCGGAAACTCCATGGCCGTGCCGTGTGTGGCTTGGCTG
GGGCACCGTCTGATACAACATCTCGCAAAAGAGGAAGCACCTGGCGCGTATTGA

Upstream 100 bases:

>100_bases
GCCGAAGGCCTAAGGGTCTTCGGCTTCTTTCTCCTGCCTTACCACTACCTCCCGGGCAAGCCGATGTCAGTGTTGGCCCT
TACGAATAGGAGAAACCCAA

Downstream 100 bases:

>100_bases
TGCGCGATTTATTGGACTCGCAAGGCCACCCTATAGCCCTGACTTGCTGAAACTTCAGCAACCGCCAGCAGCCAGGGTTT
GCAGGCTGCCGTGGGACTTC

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.NgoBV; Cytosine-specific methyltransferase NgoBV; M.NgoV [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MHPQPTHTTLQYGSVCSGIEAVSLAWQPQGPQAAWFSEIEPFPCAVLAHRYPDVPNLGDMTQIAEQVRAGLVVAPDILVG
GTPCQTFSIAGARQGLSDPRGALTLKYVELANAIDQTRQKNQRPPATIIWENVPGVLSDRSNAFGCLLGALAGESRALEP
PGAKWTHAGYVSGPQRRLAWRVLDAQHFGVAQRRKRVFLVASGRNDVDPAEVLFERDGLHGDSSQGFAPWQDAACAAGAS
TASPGDYVGLNQHYDKVRVTFGFGGGNTSGPIDVAACLTAAAGPKNDFEVETFVVQSVAGDISHTLGTSTGSSEDGTGRG
VPIITTYARQAMAFAQNSRGELRLESGHGQIAGALSTGGGKPGQGLPMVLSVALRGRDHGIAAELGGTVAPALRASSGGG
DKNHVLIPDYDTYFQYTGNRPSASDWSQWRVRRLMPVECERLQGMPDDYTQVPYRGKPATDTQRYKAIGNSMAVPCVAWL
GHRLIQHLAKEEAPGAY

Sequences:

>Translated_497_residues
MHPQPTHTTLQYGSVCSGIEAVSLAWQPQGPQAAWFSEIEPFPCAVLAHRYPDVPNLGDMTQIAEQVRAGLVVAPDILVG
GTPCQTFSIAGARQGLSDPRGALTLKYVELANAIDQTRQKNQRPPATIIWENVPGVLSDRSNAFGCLLGALAGESRALEP
PGAKWTHAGYVSGPQRRLAWRVLDAQHFGVAQRRKRVFLVASGRNDVDPAEVLFERDGLHGDSSQGFAPWQDAACAAGAS
TASPGDYVGLNQHYDKVRVTFGFGGGNTSGPIDVAACLTAAAGPKNDFEVETFVVQSVAGDISHTLGTSTGSSEDGTGRG
VPIITTYARQAMAFAQNSRGELRLESGHGQIAGALSTGGGKPGQGLPMVLSVALRGRDHGIAAELGGTVAPALRASSGGG
DKNHVLIPDYDTYFQYTGNRPSASDWSQWRVRRLMPVECERLQGMPDDYTQVPYRGKPATDTQRYKAIGNSMAVPCVAWL
GHRLIQHLAKEEAPGAY
>Mature_497_residues
MHPQPTHTTLQYGSVCSGIEAVSLAWQPQGPQAAWFSEIEPFPCAVLAHRYPDVPNLGDMTQIAEQVRAGLVVAPDILVG
GTPCQTFSIAGARQGLSDPRGALTLKYVELANAIDQTRQKNQRPPATIIWENVPGVLSDRSNAFGCLLGALAGESRALEP
PGAKWTHAGYVSGPQRRLAWRVLDAQHFGVAQRRKRVFLVASGRNDVDPAEVLFERDGLHGDSSQGFAPWQDAACAAGAS
TASPGDYVGLNQHYDKVRVTFGFGGGNTSGPIDVAACLTAAAGPKNDFEVETFVVQSVAGDISHTLGTSTGSSEDGTGRG
VPIITTYARQAMAFAQNSRGELRLESGHGQIAGALSTGGGKPGQGLPMVLSVALRGRDHGIAAELGGTVAPALRASSGGG
DKNHVLIPDYDTYFQYTGNRPSASDWSQWRVRRLMPVECERLQGMPDDYTQVPYRGKPATDTQRYKAIGNSMAVPCVAWL
GHRLIQHLAKEEAPGAY

Specific function: This methylase recognizes the double-stranded sequence GGNNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBV endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 52819; Mature: 52819

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPQPTHTTLQYGSVCSGIEAVSLAWQPQGPQAAWFSEIEPFPCAVLAHRYPDVPNLGDM
CCCCCCCCCEECCHHHCCCCEEEEEECCCCCCCHHHHCCCCCCHHHHHHCCCCCCCCCCH
TQIAEQVRAGLVVAPDILVGGTPCQTFSIAGARQGLSDPRGALTLKYVELANAIDQTRQK
HHHHHHHHCCEEECCCEEECCCCCCEEEECHHHCCCCCCCCCEEEHHHHHHHHHHHHHHH
NQRPPATIIWENVPGVLSDRSNAFGCLLGALAGESRALEPPGAKWTHAGYVSGPQRRLAW
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RVLDAQHFGVAQRRKRVFLVASGRNDVDPAEVLFERDGLHGDSSQGFAPWQDAACAAGAS
HHHCHHHCCHHHHCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCC
TASPGDYVGLNQHYDKVRVTFGFGGGNTSGPIDVAACLTAAAGPKNDFEVETFVVQSVAG
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
DISHTLGTSTGSSEDGTGRGVPIITTYARQAMAFAQNSRGELRLESGHGQIAGALSTGGG
HHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCC
KPGQGLPMVLSVALRGRDHGIAAELGGTVAPALRASSGGGDKNHVLIPDYDTYFQYTGNR
CCCCCCHHHHHHHHCCCCCCCHHHHCCCHHHHHHCCCCCCCCCEEECCCCHHHEEECCCC
PSASDWSQWRVRRLMPVECERLQGMPDDYTQVPYRGKPATDTQRYKAIGNSMAVPCVAWL
CCCCCHHHHHHHHCCCCCHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHH
GHRLIQHLAKEEAPGAY
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHPQPTHTTLQYGSVCSGIEAVSLAWQPQGPQAAWFSEIEPFPCAVLAHRYPDVPNLGDM
CCCCCCCCCEECCHHHCCCCEEEEEECCCCCCCHHHHCCCCCCHHHHHHCCCCCCCCCCH
TQIAEQVRAGLVVAPDILVGGTPCQTFSIAGARQGLSDPRGALTLKYVELANAIDQTRQK
HHHHHHHHCCEEECCCEEECCCCCCEEEECHHHCCCCCCCCCEEEHHHHHHHHHHHHHHH
NQRPPATIIWENVPGVLSDRSNAFGCLLGALAGESRALEPPGAKWTHAGYVSGPQRRLAW
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RVLDAQHFGVAQRRKRVFLVASGRNDVDPAEVLFERDGLHGDSSQGFAPWQDAACAAGAS
HHHCHHHCCHHHHCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCC
TASPGDYVGLNQHYDKVRVTFGFGGGNTSGPIDVAACLTAAAGPKNDFEVETFVVQSVAG
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
DISHTLGTSTGSSEDGTGRGVPIITTYARQAMAFAQNSRGELRLESGHGQIAGALSTGGG
HHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCC
KPGQGLPMVLSVALRGRDHGIAAELGGTVAPALRASSGGGDKNHVLIPDYDTYFQYTGNR
CCCCCCHHHHHHHHCCCCCCCHHHHCCCHHHHHHCCCCCCCCCEEECCCCHHHEEECCCC
PSASDWSQWRVRRLMPVECERLQGMPDDYTQVPYRGKPATDTQRYKAIGNSMAVPCVAWL
CCCCCHHHHHHHHCCCCCHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHH
GHRLIQHLAKEEAPGAY
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]