Click here to switch to the map view.

The map label for this gene is merA [H]

Identifier: 114330063

GI number: 114330063

Start: 32984

End: 34687

Strand: Reverse

Name: merA [H]

Synonym: Neut_0030

Alternate gene names: 114330063

Gene position: 34687-32984 (Counterclockwise)

Preceding gene: 114330064

Following gene: 114330062

Centisome position: 1.3

GC content: 61.8

Gene sequence:

>1704_bases
GTGAAAACACCAAGTCGATGCCTGTTGCACCCCTCAATCTACTGGAAAAAAATGACTGAAATCACCGTGAATGGTATGAC
CTGTATATCCTGTGCCACCGATGTCAAAGTTGCTTTGGAAAAAATTCCCGGCGTGAATGGCGCTGTGGTGTCCTATCCCG
AAAGTCGCGCGCGGGTCTCGGCAGACGCTGCCGTGGCCGTGAGCCATGACCAAATTCTGGCCGCTATCGCGGCATTGGGT
TATCAAGGTTCGATCCGCGTTGGCGATTTCAAAAGTGATACGAAAAGTCGTGATGCTCTTGAGGGGGCCGGTTTACACAT
TGCCGTCATTGGCAGCGGTGGAGCCGCGATGGCGGCAGCACTGAAGGCCGTCGAGCAAGGCGCGCACGTCACGCTGATCG
AGCGCGGCACCATCGGCGGCACCTGCGTCAATATCGGCTGTGTGCCGTCCAAAATCATGATCCGCGCTGCCCAGATTGCC
CATCTGCGCTGCGAAAGCCCGTTCGATGGCGGTATCGCGGCTACTGTGCCTGCGATTGATCGTAGCAAACTACTGGCCCA
GCAGCAGGCCCGCGTCGATGAGCTGCGCCACGCCAAGTATGAAGACATCCTGGACGGCAATCCGGCCATCACCGTTCTGC
ACGGTGAAGCGCGTTTCAAGGACGACCAGCGCCTAGTCGTCCGTTTGAACGGTGGTGGCGAGCGTGTAGTGGCGTTCGAC
CGCTGCCTGGTCGCCGCGGGTGCCAGCCCGGCAGTGCCGCCGATTCCGGTCTTGAAAGAGTCACCCTACTGGACTTCGAC
CGAAGCCCTGATCAGCGACACCGTTCCCGAACGTCTGGCCGTAATCGGATCCTCGGTGGTGGCGCTGGAATTGGCGCAGG
CTTTCGCCCGGCTGGGTAGCCAGGTCACGATCTTGGCACGCAACACGCTGTTCTTCCGCGAAGACCCTGCCATTGGCGAG
GCGGTCACAGCCGCCTTCCGTACCGAAGGAGTTCAGGTGCTGGAACACACGCAAGCCAGCCGGGTCGCCCATGTTGACGG
CGAATTCGTGTTGACCACGGGGTTCGGTGAAATACTCGCCGACAAGCTGCTGGTCGCTACCGGACGGACGCCGAACACGC
GCAGCCTGGAATTGGAAGCGGCGGGTGTCGCTGTCAATGCCCAAGGGGCCATCGTCGTCGACCAGGGCATGCGCACGAGC
AACCCGAACATCTACGCGGCTGGCGACTGCACCGACCAGCCCCAATTCGTCTATGTGGCGGCAGCGGCCGGCACCCGTGC
CGCGATTAACATGACTGGCGGCGACGCGGCCCTCGATCTGACCGCGATGCCGGCCGTGGTGTTCACCGATCCGCAAGTGG
CGACCGTGGGCTACAGCGAAGCGGAAGCGCATCACGACGGGATCGAGACCGACAGCCGGTTGCTCACGCTGGACAACGTG
CCGCGTGCGCTCGCCAACTTCGACACGCTGGGCTTCATCAAGCTGGTGGCCGATGCCGGCAGCGGGCGCTTGATCGGCGT
GCAGGTAGTGGCACCGGAAGCGGGCGAGCTGATCCAGACGGCGGCCTTGGCAATTCACAACCGCATGACAGTGCAGGAAC
TGGCCGACCAGTTGTTCCCCTATCTGACGATGGTCGAGGGGCTGAAGCTCGCGGCACAGACCTTTAGCAAGGACGTGAAG
CAACTGTCCTGCTGCGCCGGATGA

Upstream 100 bases:

>100_bases
TGCTCGGGCTGGTCGGTTTGTTCGCTCTCACCGACTATCTGAACAGTGTGCTGCTGCCTGCGCTGACGATTTTCATTGGT
TTAACCATCTACGATATCCA

Downstream 100 bases:

>100_bases
GGAAAAGGAGGTGCTCAATGAGCGCCTACACAGTGTCCCGGCTGACCCTTGATGCGGGGGTGAGCGTGTATATCGTGCGC
GACTATCTGCTGCGCGGATT

Product: putative mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MKTPSRCLLHPSIYWKKMTEITVNGMTCISCATDVKVALEKIPGVNGAVVSYPESRARVSADAAVAVSHDQILAAIAALG
YQGSIRVGDFKSDTKSRDALEGAGLHIAVIGSGGAAMAAALKAVEQGAHVTLIERGTIGGTCVNIGCVPSKIMIRAAQIA
HLRCESPFDGGIAATVPAIDRSKLLAQQQARVDELRHAKYEDILDGNPAITVLHGEARFKDDQRLVVRLNGGGERVVAFD
RCLVAAGASPAVPPIPVLKESPYWTSTEALISDTVPERLAVIGSSVVALELAQAFARLGSQVTILARNTLFFREDPAIGE
AVTAAFRTEGVQVLEHTQASRVAHVDGEFVLTTGFGEILADKLLVATGRTPNTRSLELEAAGVAVNAQGAIVVDQGMRTS
NPNIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLTAMPAVVFTDPQVATVGYSEAEAHHDGIETDSRLLTLDNV
PRALANFDTLGFIKLVADAGSGRLIGVQVVAPEAGELIQTAALAIHNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVK
QLSCCAG

Sequences:

>Translated_567_residues
MKTPSRCLLHPSIYWKKMTEITVNGMTCISCATDVKVALEKIPGVNGAVVSYPESRARVSADAAVAVSHDQILAAIAALG
YQGSIRVGDFKSDTKSRDALEGAGLHIAVIGSGGAAMAAALKAVEQGAHVTLIERGTIGGTCVNIGCVPSKIMIRAAQIA
HLRCESPFDGGIAATVPAIDRSKLLAQQQARVDELRHAKYEDILDGNPAITVLHGEARFKDDQRLVVRLNGGGERVVAFD
RCLVAAGASPAVPPIPVLKESPYWTSTEALISDTVPERLAVIGSSVVALELAQAFARLGSQVTILARNTLFFREDPAIGE
AVTAAFRTEGVQVLEHTQASRVAHVDGEFVLTTGFGEILADKLLVATGRTPNTRSLELEAAGVAVNAQGAIVVDQGMRTS
NPNIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLTAMPAVVFTDPQVATVGYSEAEAHHDGIETDSRLLTLDNV
PRALANFDTLGFIKLVADAGSGRLIGVQVVAPEAGELIQTAALAIHNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVK
QLSCCAG
>Mature_567_residues
MKTPSRCLLHPSIYWKKMTEITVNGMTCISCATDVKVALEKIPGVNGAVVSYPESRARVSADAAVAVSHDQILAAIAALG
YQGSIRVGDFKSDTKSRDALEGAGLHIAVIGSGGAAMAAALKAVEQGAHVTLIERGTIGGTCVNIGCVPSKIMIRAAQIA
HLRCESPFDGGIAATVPAIDRSKLLAQQQARVDELRHAKYEDILDGNPAITVLHGEARFKDDQRLVVRLNGGGERVVAFD
RCLVAAGASPAVPPIPVLKESPYWTSTEALISDTVPERLAVIGSSVVALELAQAFARLGSQVTILARNTLFFREDPAIGE
AVTAAFRTEGVQVLEHTQASRVAHVDGEFVLTTGFGEILADKLLVATGRTPNTRSLELEAAGVAVNAQGAIVVDQGMRTS
NPNIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLTAMPAVVFTDPQVATVGYSEAEAHHDGIETDSRLLTLDNV
PRALANFDTLGFIKLVADAGSGRLIGVQVVAPEAGELIQTAALAIHNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVK
QLSCCAG

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=472, Percent_Identity=29.2372881355932, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI50301238, Length=435, Percent_Identity=28.0459770114943, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI22035672, Length=406, Percent_Identity=30.2955665024631, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI33519430, Length=420, Percent_Identity=27.1428571428571, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI33519428, Length=420, Percent_Identity=27.1428571428571, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI33519426, Length=420, Percent_Identity=27.1428571428571, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI148277065, Length=420, Percent_Identity=27.1428571428571, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI148277071, Length=420, Percent_Identity=27.1428571428571, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI291045266, Length=420, Percent_Identity=27.1428571428571, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI291045268, Length=414, Percent_Identity=26.3285024154589, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI87081717, Length=447, Percent_Identity=30.2013422818792, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=30.5555555555556, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1789915, Length=415, Percent_Identity=32.7710843373494, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87082354, Length=456, Percent_Identity=24.5614035087719, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789065, Length=218, Percent_Identity=27.5229357798165, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=460, Percent_Identity=27.3913043478261, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI71983419, Length=350, Percent_Identity=32.2857142857143, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=350, Percent_Identity=32.2857142857143, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17557007, Length=505, Percent_Identity=26.9306930693069, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=469, Percent_Identity=28.1449893390192, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6325166, Length=470, Percent_Identity=25.7446808510638, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=24.7881355932203, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21358499, Length=483, Percent_Identity=30.8488612836439, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24640553, Length=489, Percent_Identity=29.2433537832311, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24640549, Length=489, Percent_Identity=29.2433537832311, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24640551, Length=489, Percent_Identity=29.2433537832311, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI17737741, Length=494, Percent_Identity=27.9352226720648, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 59479; Mature: 59479

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS01047 HMA_1 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPSRCLLHPSIYWKKMTEITVNGMTCISCATDVKVALEKIPGVNGAVVSYPESRARVS
CCCCCCEEECCHHHHEEEEEEEECCEEEEEHHHHHHHHHHHCCCCCCEEEECCCHHHEEC
ADAAVAVSHDQILAAIAALGYQGSIRVGDFKSDTKSRDALEGAGLHIAVIGSGGAAMAAA
CCEEEEECHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHCCCCEEEEEEECCCHHHHHH
LKAVEQGAHVTLIERGTIGGTCVNIGCVPSKIMIRAAQIAHLRCESPFDGGIAATVPAID
HHHHHCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHEEEECCCCCCCEEEEECCCC
RSKLLAQQQARVDELRHAKYEDILDGNPAITVLHGEARFKDDQRLVVRLNGGGERVVAFD
HHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEE
RCLVAAGASPAVPPIPVLKESPYWTSTEALISDTVPERLAVIGSSVVALELAQAFARLGS
EEHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHCCC
QVTILARNTLFFREDPAIGEAVTAAFRTEGVQVLEHTQASRVAHVDGEFVLTTGFGEILA
EEEEEEECEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCEEECCCCEEEECCCHHHHH
DKLLVATGRTPNTRSLELEAAGVAVNAQGAIVVDQGMRTSNPNIYAAGDCTDQPQFVYVA
HHEEEEECCCCCCCEEEEEECCEEEECCCEEEEECCCCCCCCCEEECCCCCCCCCEEEEE
AAAGTRAAINMTGGDAALDLTAMPAVVFTDPQVATVGYSEAEAHHDGIETDSRLLTLDNV
ECCCCEEEEEECCCCCEEEEEECCEEEEECCCEEEECCCHHHHHCCCCCCCCEEEEECCC
PRALANFDTLGFIKLVADAGSGRLIGVQVVAPEAGELIQTAALAIHNRMTVQELADQLFP
CHHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
YLTMVEGLKLAAQTFSKDVKQLSCCAG
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTPSRCLLHPSIYWKKMTEITVNGMTCISCATDVKVALEKIPGVNGAVVSYPESRARVS
CCCCCCEEECCHHHHEEEEEEEECCEEEEEHHHHHHHHHHHCCCCCCEEEECCCHHHEEC
ADAAVAVSHDQILAAIAALGYQGSIRVGDFKSDTKSRDALEGAGLHIAVIGSGGAAMAAA
CCEEEEECHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHCCCCEEEEEEECCCHHHHHH
LKAVEQGAHVTLIERGTIGGTCVNIGCVPSKIMIRAAQIAHLRCESPFDGGIAATVPAID
HHHHHCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHEEEECCCCCCCEEEEECCCC
RSKLLAQQQARVDELRHAKYEDILDGNPAITVLHGEARFKDDQRLVVRLNGGGERVVAFD
HHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEE
RCLVAAGASPAVPPIPVLKESPYWTSTEALISDTVPERLAVIGSSVVALELAQAFARLGS
EEHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHCCC
QVTILARNTLFFREDPAIGEAVTAAFRTEGVQVLEHTQASRVAHVDGEFVLTTGFGEILA
EEEEEEECEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCEEECCCCEEEECCCHHHHH
DKLLVATGRTPNTRSLELEAAGVAVNAQGAIVVDQGMRTSNPNIYAAGDCTDQPQFVYVA
HHEEEEECCCCCCCEEEEEECCEEEECCCEEEEECCCCCCCCCEEECCCCCCCCCEEEEE
AAAGTRAAINMTGGDAALDLTAMPAVVFTDPQVATVGYSEAEAHHDGIETDSRLLTLDNV
ECCCCEEEEEECCCCCEEEEEECCEEEEECCCEEEECCCHHHHHCCCCCCCCEEEEECCC
PRALANFDTLGFIKLVADAGSGRLIGVQVVAPEAGELIQTAALAIHNRMTVQELADQLFP
CHHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
YLTMVEGLKLAAQTFSKDVKQLSCCAG
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063107 [H]