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The map label for this gene is nemA [H]

Identifier: 113969502

GI number: 113969502

Start: 1360600

End: 1361667

Strand: Direct

Name: nemA [H]

Synonym: Shewmr4_1158

Alternate gene names: 113969502

Gene position: 1360600-1361667 (Clockwise)

Preceding gene: 113969500

Following gene: 113969504

Centisome position: 28.91

GC content: 49.72

Gene sequence:

>1068_bases
ATGACGATTGAACATGCAGCGAATTCCGTGGGTAATTTATTCGATACCTACACACTTAATGACACTATTACCTTAAAAAA
CCGTATTTTAATGGCGCCATTAACGCGCTGTATGGCCGATGCGGATTTAGTACCGACCGATGACATGGTGGCTTACTATG
CTCGCCGCGCCGAAGCGGGATTGATTATCTCCGAAGCGACGATCATTCGCCCTGATGGTCAAGGTTACCCAAATACGCCC
GGCATTTTTAATCAAGCGCAGATCGCCGGTTGGCGTAAAGTGACGGATGCTGTGCATGCCAACGGCGGCAAGATTTTTGT
GCAGTTATGGCATACTGGCCGTGTCGCGCACCCACATTTCTTCGGTGGCGGCGATGTACTGGCGCCTTCGGCTGAAAAAG
TAGAGGGCAGTGTGCCCAGAATGCGTGAGCTGACCTATGTCACTCCAAAAGCGGCAACTCTTGAAGATATTCAGGGTTTA
GTCCGCGATTATGCGAAAGCGGCCGAAAACGCGATTGAAGCCGGATTCGATGGGGTTGAGATCCACGGTGCAAACGGATA
TTTAATCGATGCATTCTTACACCATGACAGCAACCGTCGTACCGATGAATACGGCGGCACACCGGCCAATATGTCACGCT
TTGCCTTAGAAGTGGTTGATGCGATTGCGGCGCGTATTGGTAAAGACAGAACCGGTCTTCGAATTTCTCCCGGCGCCTAT
TTCAATATGGCTGTCGATAACCGTGACCGCGCCGTGTTTGATTACCTGTTACCTGAGCTCGAAAAACGTGAACTGGCCTT
TGTGCATATCGGTATCTTCGACGACAGCATGGAGTTTGATTACTTAGATGGCCGTGTTTCTAGCTATGTGCGTGCTAACT
ATGGCAAAACCTTAGTGGGTGTAGGTGGTTACAGCGCCCAATCGGCAAGTGAAGCGATTGCCGCCGATAAGTTTGATTTA
ATTGCGATTGGTCGTCCCTTTATTGCCAACCCAGATTATGTGGCAAGAGTGCGCCAAGGTCATGAATTGGTGACCTATAG
CGATGAAATGCTGGCAAGCTTGGTGTAA

Upstream 100 bases:

>100_bases
GCTGATTCTAACTAATTTTGAACAAACATTCAAAAATGTTCTTGAATGATCGTTCAAATAAGAATATCTTGAATGCACAT
TCAAGAATGGGGCAACAGCA

Downstream 100 bases:

>100_bases
CTCAGAGCAGCAATTACCGATAAGAAAGGGTTAGCTTAGCTAACCCTTTTTGCTTTTGGTCCAACTATCAATTGGCTTTA
GGGAGCGCCCTCAGGGCATT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTIEHAANSVGNLFDTYTLNDTITLKNRILMAPLTRCMADADLVPTDDMVAYYARRAEAGLIISEATIIRPDGQGYPNTP
GIFNQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAPSAEKVEGSVPRMRELTYVTPKAATLEDIQGL
VRDYAKAAENAIEAGFDGVEIHGANGYLIDAFLHHDSNRRTDEYGGTPANMSRFALEVVDAIAARIGKDRTGLRISPGAY
FNMAVDNRDRAVFDYLLPELEKRELAFVHIGIFDDSMEFDYLDGRVSSYVRANYGKTLVGVGGYSAQSASEAIAADKFDL
IAIGRPFIANPDYVARVRQGHELVTYSDEMLASLV

Sequences:

>Translated_355_residues
MTIEHAANSVGNLFDTYTLNDTITLKNRILMAPLTRCMADADLVPTDDMVAYYARRAEAGLIISEATIIRPDGQGYPNTP
GIFNQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAPSAEKVEGSVPRMRELTYVTPKAATLEDIQGL
VRDYAKAAENAIEAGFDGVEIHGANGYLIDAFLHHDSNRRTDEYGGTPANMSRFALEVVDAIAARIGKDRTGLRISPGAY
FNMAVDNRDRAVFDYLLPELEKRELAFVHIGIFDDSMEFDYLDGRVSSYVRANYGKTLVGVGGYSAQSASEAIAADKFDL
IAIGRPFIANPDYVARVRQGHELVTYSDEMLASLV
>Mature_354_residues
TIEHAANSVGNLFDTYTLNDTITLKNRILMAPLTRCMADADLVPTDDMVAYYARRAEAGLIISEATIIRPDGQGYPNTPG
IFNQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAPSAEKVEGSVPRMRELTYVTPKAATLEDIQGLV
RDYAKAAENAIEAGFDGVEIHGANGYLIDAFLHHDSNRRTDEYGGTPANMSRFALEVVDAIAARIGKDRTGLRISPGAYF
NMAVDNRDRAVFDYLLPELEKRELAFVHIGIFDDSMEFDYLDGRVSSYVRANYGKTLVGVGGYSAQSASEAIAADKFDLI
AIGRPFIANPDYVARVRQGHELVTYSDEMLASLV

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=331, Percent_Identity=43.202416918429, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1789463, Length=344, Percent_Identity=29.6511627906977, Blast_Score=121, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17559804, Length=355, Percent_Identity=27.0422535211268, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17565138, Length=348, Percent_Identity=27.8735632183908, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI72001454, Length=350, Percent_Identity=26.5714285714286, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=353, Percent_Identity=27.7620396600567, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=359, Percent_Identity=25.9052924791086, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=363, Percent_Identity=25.8953168044077, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=363, Percent_Identity=37.1900826446281, Blast_Score=196, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6321973, Length=363, Percent_Identity=35.5371900826446, Blast_Score=183, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38855; Mature: 38724

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEHAANSVGNLFDTYTLNDTITLKNRILMAPLTRCMADADLVPTDDMVAYYARRAEAG
CCCHHHHHHHHHHHEEEECCCEEEECCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
LIISEATIIRPDGQGYPNTPGIFNQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHF
EEEECEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
FGGGDVLAPSAEKVEGSVPRMRELTYVTPKAATLEDIQGLVRDYAKAAENAIEAGFDGVE
ECCCCEECCCHHHHCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGANGYLIDAFLHHDSNRRTDEYGGTPANMSRFALEVVDAIAARIGKDRTGLRISPGAY
EECCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
FNMAVDNRDRAVFDYLLPELEKRELAFVHIGIFDDSMEFDYLDGRVSSYVRANYGKTLVG
EEEEECCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEECCCHHHHHHHHCCCCEEEE
VGGYSAQSASEAIAADKFDLIAIGRPFIANPDYVARVRQGHELVTYSDEMLASLV
ECCCCCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCEEEEECHHHHHHCC
>Mature Secondary Structure 
TIEHAANSVGNLFDTYTLNDTITLKNRILMAPLTRCMADADLVPTDDMVAYYARRAEAG
CCHHHHHHHHHHHEEEECCCEEEECCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
LIISEATIIRPDGQGYPNTPGIFNQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHF
EEEECEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
FGGGDVLAPSAEKVEGSVPRMRELTYVTPKAATLEDIQGLVRDYAKAAENAIEAGFDGVE
ECCCCEECCCHHHHCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGANGYLIDAFLHHDSNRRTDEYGGTPANMSRFALEVVDAIAARIGKDRTGLRISPGAY
EECCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
FNMAVDNRDRAVFDYLLPELEKRELAFVHIGIFDDSMEFDYLDGRVSSYVRANYGKTLVG
EEEEECCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEECCCHHHHHHHHCCCCEEEE
VGGYSAQSASEAIAADKFDLIAIGRPFIANPDYVARVRQGHELVTYSDEMLASLV
ECCCCCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCEEEEECHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]