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The map label for this gene is rumA [H]

Identifier: 113969454

GI number: 113969454

Start: 1302286

End: 1303677

Strand: Direct

Name: rumA [H]

Synonym: Shewmr4_1110

Alternate gene names: 113969454

Gene position: 1302286-1303677 (Clockwise)

Preceding gene: 113969450

Following gene: 113969455

Centisome position: 27.67

GC content: 49.71

Gene sequence:

>1392_bases
ATGGGCGGTCTTTACCAGTTTTTTATTGATTTAGAGGCCGAAATGGCACAATTTTTTAAAGCAAAACCAAATAGTTCCAA
GCAATTGTCCGCGAAGCAGTCCTTTAGCGTGCACCAGCTCGATCATCTAGGGGCAGGTATAGCACAACATCAGGGAAAAG
TCGTATTTATCCCCGGCGCCTTGCCCAGCGAAACCGTGCAGGCGCAGCTGACCGAACAAAAGAAAAACTATGCCCGAGCC
AAGCTAATTAAAGTCGAGACGCCGAGCGCTGAGCGAGTCACGCCGCTATGCCCACATTACCAAAGCTGTGGCGGCTGCGA
TTTACAGCATATGTCATTAGCTGGGCAACGCGAGCATAAGTCTGCGGCCTTGGTTGATATTATGGCTAAATTTGCTGGCG
CCGAAGGCAACTCAGTGCCCGCCTTGACGGGTGAGGGGTGGCACTATCGTCGCCGTGCCCGCCTTGCGACCCTATTTGAT
AAAAATACCAAGCAGTTGAGCCTAGGGTTTCGTGCCTCGAGCAGCAACCAAGTCGTGCCTATTGATAGCTGTTTAGTCTT
GGCGAAACCCTTATCGGATTTAATTGCCCCGTTTGCTAAGTTGCTTAATCAATTGGCGGCCAAATCGAGTCTTGGGCATT
TAGAGTTGATTGATGCCGACAACGGCCATTTTGCGGTAATTCGGATCACTAAATCCTTAAATGATAAGGACATGGCTAAG
CTTGCGCAGTTTGCCGAACATCATCAAATCCATATTTGTTTGCAAGACAACAACGGCGAGTTCCATGGGGTGAATGGCAC
GCTGTTATTGCCCGTTTACCAGTTGCTCGATGATAAAGCTGGTGCAACGCCTGTGAGCCTCACCTTCACCCCGGGTAACT
TTGTGCAAGTGAATGCGCAAATTAACAAGGCCATGGTGGCGCAGGCGTTAGACTGGTTAGCGCCGCAGCCGGGTGAGCGA
ATTCTCGATCTCTTCTGTGGTATGGGGAATTTCAGTTTGCCATTAGCTAAACTGGGCGCCGAAGTGATTGGGGTTGAAGG
GGTGCCAGATATGGTTAGCCAAGCCCGTGAAAACGCGGCGGCAAATGGCTTAAGCAATTTAACCTTTTACCATGGCGATT
TAAGTGCCGATTTATCCTGCGAGCCTTGGATGGGCAAGATTGATAAGTTGCTACTCGATCCTGCCCGCGCGGGGGCGTTT
GAGAGTTTGCAATGGCTTAAGAAGATGAAACCTCGCCAAGTAGTGTATGTGTCTTGTAATCCGGCGAGCTTGGCCCGCGA
CAGTGCGGTGTTACTTGAACGTGGCTACAAGTTACAAAAGTTAGGTTTAATCGATATGTTCCCGCAGACACATCATATCG
AAGCCATGGCGTTGTTTGAGCTTGCAAAATAA

Upstream 100 bases:

>100_bases
GAACGTAAGCTGTATTTGGTCATGTTGTTGACAGGGTTCATAGCCGCAGCGCAGTTTCCTAAATTCTAGGGACGATATGA
TTTTATAAGACTGCCAGAAA

Downstream 100 bases:

>100_bases
AGCTTATCGGTTGGGCAAGGTTTCTTCACTTGCCCGCGTTATTCGAGTGTAAAGTAAGCAAAGCGTTTGAATAACGTAAA
AAATGTGAAGTTTCGTCTCG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MGGLYQFFIDLEAEMAQFFKAKPNSSKQLSAKQSFSVHQLDHLGAGIAQHQGKVVFIPGALPSETVQAQLTEQKKNYARA
KLIKVETPSAERVTPLCPHYQSCGGCDLQHMSLAGQREHKSAALVDIMAKFAGAEGNSVPALTGEGWHYRRRARLATLFD
KNTKQLSLGFRASSSNQVVPIDSCLVLAKPLSDLIAPFAKLLNQLAAKSSLGHLELIDADNGHFAVIRITKSLNDKDMAK
LAQFAEHHQIHICLQDNNGEFHGVNGTLLLPVYQLLDDKAGATPVSLTFTPGNFVQVNAQINKAMVAQALDWLAPQPGER
ILDLFCGMGNFSLPLAKLGAEVIGVEGVPDMVSQARENAAANGLSNLTFYHGDLSADLSCEPWMGKIDKLLLDPARAGAF
ESLQWLKKMKPRQVVYVSCNPASLARDSAVLLERGYKLQKLGLIDMFPQTHHIEAMALFELAK

Sequences:

>Translated_463_residues
MGGLYQFFIDLEAEMAQFFKAKPNSSKQLSAKQSFSVHQLDHLGAGIAQHQGKVVFIPGALPSETVQAQLTEQKKNYARA
KLIKVETPSAERVTPLCPHYQSCGGCDLQHMSLAGQREHKSAALVDIMAKFAGAEGNSVPALTGEGWHYRRRARLATLFD
KNTKQLSLGFRASSSNQVVPIDSCLVLAKPLSDLIAPFAKLLNQLAAKSSLGHLELIDADNGHFAVIRITKSLNDKDMAK
LAQFAEHHQIHICLQDNNGEFHGVNGTLLLPVYQLLDDKAGATPVSLTFTPGNFVQVNAQINKAMVAQALDWLAPQPGER
ILDLFCGMGNFSLPLAKLGAEVIGVEGVPDMVSQARENAAANGLSNLTFYHGDLSADLSCEPWMGKIDKLLLDPARAGAF
ESLQWLKKMKPRQVVYVSCNPASLARDSAVLLERGYKLQKLGLIDMFPQTHHIEAMALFELAK
>Mature_462_residues
GGLYQFFIDLEAEMAQFFKAKPNSSKQLSAKQSFSVHQLDHLGAGIAQHQGKVVFIPGALPSETVQAQLTEQKKNYARAK
LIKVETPSAERVTPLCPHYQSCGGCDLQHMSLAGQREHKSAALVDIMAKFAGAEGNSVPALTGEGWHYRRRARLATLFDK
NTKQLSLGFRASSSNQVVPIDSCLVLAKPLSDLIAPFAKLLNQLAAKSSLGHLELIDADNGHFAVIRITKSLNDKDMAKL
AQFAEHHQIHICLQDNNGEFHGVNGTLLLPVYQLLDDKAGATPVSLTFTPGNFVQVNAQINKAMVAQALDWLAPQPGERI
LDLFCGMGNFSLPLAKLGAEVIGVEGVPDMVSQARENAAANGLSNLTFYHGDLSADLSCEPWMGKIDKLLLDPARAGAFE
SLQWLKKMKPRQVVYVSCNPASLARDSAVLLERGYKLQKLGLIDMFPQTHHIEAMALFELAK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=199, Percent_Identity=34.6733668341709, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI51173878, Length=199, Percent_Identity=34.6733668341709, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789148, Length=452, Percent_Identity=44.6902654867257, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1787083, Length=379, Percent_Identity=26.1213720316623, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790403, Length=183, Percent_Identity=30.6010928961749, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24666579, Length=228, Percent_Identity=32.4561403508772, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 50473; Mature: 50342

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGLYQFFIDLEAEMAQFFKAKPNSSKQLSAKQSFSVHQLDHLGAGIAQHQGKVVFIPGA
CCCCEEEEEEHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHCCCHHHHCCCEEEECCC
LPSETVQAQLTEQKKNYARAKLIKVETPSAERVTPLCPHYQSCGGCDLQHMSLAGQREHK
CCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHH
SAALVDIMAKFAGAEGNSVPALTGEGWHYRRRARLATLFDKNTKQLSLGFRASSSNQVVP
HHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEE
IDSCLVLAKPLSDLIAPFAKLLNQLAAKSSLGHLELIDADNGHFAVIRITKSLNDKDMAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCHHHHHH
LAQFAEHHQIHICLQDNNGEFHGVNGTLLLPVYQLLDDKAGATPVSLTFTPGNFVQVNAQ
HHHHHCCCEEEEEEECCCCCEECCCCEEHHHHHHHHCCCCCCCEEEEEECCCCEEEEECH
INKAMVAQALDWLAPQPGERILDLFCGMGNFSLPLAKLGAEVIGVEGVPDMVSQARENAA
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHCCCEEECCCCHHHHHHHHHHHH
ANGLSNLTFYHGDLSADLSCEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRQVVYVSCN
HCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECC
PASLARDSAVLLERGYKLQKLGLIDMFPQTHHIEAMALFELAK
CHHHHCCHHHHHHCCCCEEECCHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
GGLYQFFIDLEAEMAQFFKAKPNSSKQLSAKQSFSVHQLDHLGAGIAQHQGKVVFIPGA
CCCEEEEEEHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHCCCHHHHCCCEEEECCC
LPSETVQAQLTEQKKNYARAKLIKVETPSAERVTPLCPHYQSCGGCDLQHMSLAGQREHK
CCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHH
SAALVDIMAKFAGAEGNSVPALTGEGWHYRRRARLATLFDKNTKQLSLGFRASSSNQVVP
HHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEE
IDSCLVLAKPLSDLIAPFAKLLNQLAAKSSLGHLELIDADNGHFAVIRITKSLNDKDMAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCHHHHHH
LAQFAEHHQIHICLQDNNGEFHGVNGTLLLPVYQLLDDKAGATPVSLTFTPGNFVQVNAQ
HHHHHCCCEEEEEEECCCCCEECCCCEEHHHHHHHHCCCCCCCEEEEEECCCCEEEEECH
INKAMVAQALDWLAPQPGERILDLFCGMGNFSLPLAKLGAEVIGVEGVPDMVSQARENAA
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHCCCEEECCCCHHHHHHHHHHHH
ANGLSNLTFYHGDLSADLSCEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRQVVYVSCN
HCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECC
PASLARDSAVLLERGYKLQKLGLIDMFPQTHHIEAMALFELAK
CHHHHCCHHHHHHCCCCEEECCHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]