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The map label for this gene is nrfA [H]

Identifier: 113969003

GI number: 113969003

Start: 767809

End: 769230

Strand: Direct

Name: nrfA [H]

Synonym: Shewmr4_0659

Alternate gene names: 113969003

Gene position: 767809-769230 (Clockwise)

Preceding gene: 113969000

Following gene: 113969005

Centisome position: 16.31

GC content: 48.17

Gene sequence:

>1422_bases
ATGCAACACGGAGATGAGATGATGAAGAAGATGACAGGTAAGTCCTTTGCACTCAGTGCATTGGTAGCCGCGAGCTTCAT
GGCTGCGGGTGCGATGGCGAGCGATAAGACCGAGCCTCGCAACGAAGTATATAAAGACAAGTTTGCTAATCAATATAATA
GCTGGCATGACACAGCGAAAAGCGAAGAGATCACCGATGCATTAGCGGGCGATCCAAGCTTAGTGATTCTGTGGGCTGGT
TATGGTTTTGCTAAAGACTACAACGCACCACGCGGCCATATGTATGCTGTGACCGACGTGCGTAACACCCTACGTACTGG
CGCACCTACCAACGCCGAAGATGGTCCAATGCCAATGGCATGTTGGAGCTGTAAGAGCCCTGACGTACCTCGCCTGATTG
AAGAACAAGGTGAAGATGGTTACTTCAAAGGCAAGTGGGCTAAAGGTGGCCCAGAAGTGGTGAACACAATCGGTTGTAGC
GACTGCCACGAAAAAGGCACGCCAAAACTGCGTATTTCACGCCCATTCGCTGAACGTGGTATGGAAGCATTAGGCACTCC
ATTCGACAAAGCCTCGAAGAAAGACAAGCAATCTATGGTTTGCGGTCAGTGTCACGTTGAATACTACTTCGAGAAGAAAG
ATGATCGTAAAGGCTTCGTAAAATTCCCTTGGGATTCAGGTACTACTGTTGAACAAATGGAAGCTTACTACGACGCTATC
GAGTTCTCTGACTGGACTCATTCACTGTCTAAAACCCCAATGTTGAAGGCGCAGCACCCTGGTTATGAAACCTGGAAAAT
GGGCGTACACGGTAAGAACGATGTGAGCTGTGTTGACTGTCACATGCCTAAAGTGACTAACGACAAAGGTCGTAAGTACA
CAGATCATAAAGTGGGTAACCCATTCGATCGCTTCGATGAAACCTGTGCAACTTGCCATAGCCAAAGCAAAGAGTTCTTA
GAAGGCATCACTAAAGAACGTTATGCCAAAGTGAAAGAGCTGAAAGCACGTGCCGAAGGTCAATTAGTGAAAGCACACTT
CGAAGCGGCTAAAGCGTGGGAAGTAGGTGCAACTGAAGCCGAAATGAAGCCAATCCTGACTGATATCCGTCATGCTCAAT
GGCGCTGGGACTTCGCGATTGCTTCTCACGGTGTAGCGGCTCACGCTCCAGAAGAAGCACTGCGTATCCTAGGTACTGCG
GTTGATAAAGCGGCCGATGCCCGTGTGAAATTAGCCCAATTACTGGCGAAGAAAGGCGTGACTGATGCTGTTGCTATCCC
TGATATCTCCACTAAAGCCAAAGCTCAGGCAGCTCTGGGTATGGACATGGACAAGATGAATGCAGAGAAAGAAGCATTCA
AGAAAGACATGTTACCTAAGTGGGATGCAGAAGCGAAAAAACGCGAAGCAACTTATAAGTAA

Upstream 100 bases:

>100_bases
TCTAGCGCAAACTTCGCTCTAGATACCCCCTAAGAGGTATGTCTTTGGTGGGTTTAAGCCACTAACTTTGGCCTTGGAGA
TGTCGTGATTTATTAAAATA

Downstream 100 bases:

>100_bases
TTCCTGTTGAATATGAGCTAACCCCTCATAGCAAAGCCCCCGCAACTTAGGTTACGGGGGCTTTTTTTATTGCCTATGCG
TCTTATAAAATCAGTTGCTT

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MQHGDEMMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYNSWHDTAKSEEITDALAGDPSLVILWAG
YGFAKDYNAPRGHMYAVTDVRNTLRTGAPTNAEDGPMPMACWSCKSPDVPRLIEEQGEDGYFKGKWAKGGPEVVNTIGCS
DCHEKGTPKLRISRPFAERGMEALGTPFDKASKKDKQSMVCGQCHVEYYFEKKDDRKGFVKFPWDSGTTVEQMEAYYDAI
EFSDWTHSLSKTPMLKAQHPGYETWKMGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGNPFDRFDETCATCHSQSKEFL
EGITKERYAKVKELKARAEGQLVKAHFEAAKAWEVGATEAEMKPILTDIRHAQWRWDFAIASHGVAAHAPEEALRILGTA
VDKAADARVKLAQLLAKKGVTDAVAIPDISTKAKAQAALGMDMDKMNAEKEAFKKDMLPKWDAEAKKREATYK

Sequences:

>Translated_473_residues
MQHGDEMMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYNSWHDTAKSEEITDALAGDPSLVILWAG
YGFAKDYNAPRGHMYAVTDVRNTLRTGAPTNAEDGPMPMACWSCKSPDVPRLIEEQGEDGYFKGKWAKGGPEVVNTIGCS
DCHEKGTPKLRISRPFAERGMEALGTPFDKASKKDKQSMVCGQCHVEYYFEKKDDRKGFVKFPWDSGTTVEQMEAYYDAI
EFSDWTHSLSKTPMLKAQHPGYETWKMGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGNPFDRFDETCATCHSQSKEFL
EGITKERYAKVKELKARAEGQLVKAHFEAAKAWEVGATEAEMKPILTDIRHAQWRWDFAIASHGVAAHAPEEALRILGTA
VDKAADARVKLAQLLAKKGVTDAVAIPDISTKAKAQAALGMDMDKMNAEKEAFKKDMLPKWDAEAKKREATYK
>Mature_473_residues
MQHGDEMMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYNSWHDTAKSEEITDALAGDPSLVILWAG
YGFAKDYNAPRGHMYAVTDVRNTLRTGAPTNAEDGPMPMACWSCKSPDVPRLIEEQGEDGYFKGKWAKGGPEVVNTIGCS
DCHEKGTPKLRISRPFAERGMEALGTPFDKASKKDKQSMVCGQCHVEYYFEKKDDRKGFVKFPWDSGTTVEQMEAYYDAI
EFSDWTHSLSKTPMLKAQHPGYETWKMGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGNPFDRFDETCATCHSQSKEFL
EGITKERYAKVKELKARAEGQLVKAHFEAAKAWEVGATEAEMKPILTDIRHAQWRWDFAIASHGVAAHAPEEALRILGTA
VDKAADARVKLAQLLAKKGVTDAVAIPDISTKAKAQAALGMDMDKMNAEKEAFKKDMLPKWDAEAKKREATYK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=438, Percent_Identity=63.9269406392694, Blast_Score=613, Evalue=1e-176,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 52653; Mature: 52653

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHGDEMMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYNSWHDTAK
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
SEEITDALAGDPSLVILWAGYGFAKDYNAPRGHMYAVTDVRNTLRTGAPTNAEDGPMPMA
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEE
CWSCKSPDVPRLIEEQGEDGYFKGKWAKGGPEVVNTIGCSDCHEKGTPKLRISRPFAERG
EECCCCCCCCHHHHHCCCCCEEECCCCCCCHHHHHHCCCCHHHHCCCCCEEEECCHHHHC
MEALGTPFDKASKKDKQSMVCGQCHVEYYFEKKDDRKGFVKFPWDSGTTVEQMEAYYDAI
HHHHCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHH
EFSDWTHSLSKTPMLKAQHPGYETWKMGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGN
HHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCEECCCCCCC
PFDRFDETCATCHSQSKEFLEGITKERYAKVKELKARAEGQLVKAHFEAAKAWEVGATEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHH
EMKPILTDIRHAQWRWDFAIASHGVAAHAPEEALRILGTAVDKAADARVKLAQLLAKKGV
HHHHHHHHHHHHHEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TDAVAIPDISTKAKAQAALGMDMDKMNAEKEAFKKDMLPKWDAEAKKREATYK
CCEEECCCCCHHHHHHHHHCCCHHHHCHHHHHHHHHCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MQHGDEMMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYNSWHDTAK
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
SEEITDALAGDPSLVILWAGYGFAKDYNAPRGHMYAVTDVRNTLRTGAPTNAEDGPMPMA
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEE
CWSCKSPDVPRLIEEQGEDGYFKGKWAKGGPEVVNTIGCSDCHEKGTPKLRISRPFAERG
EECCCCCCCCHHHHHCCCCCEEECCCCCCCHHHHHHCCCCHHHHCCCCCEEEECCHHHHC
MEALGTPFDKASKKDKQSMVCGQCHVEYYFEKKDDRKGFVKFPWDSGTTVEQMEAYYDAI
HHHHCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHH
EFSDWTHSLSKTPMLKAQHPGYETWKMGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGN
HHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCEECCCCCCC
PFDRFDETCATCHSQSKEFLEGITKERYAKVKELKARAEGQLVKAHFEAAKAWEVGATEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHH
EMKPILTDIRHAQWRWDFAIASHGVAAHAPEEALRILGTAVDKAADARVKLAQLLAKKGV
HHHHHHHHHHHHHEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TDAVAIPDISTKAKAQAALGMDMDKMNAEKEAFKKDMLPKWDAEAKKREATYK
CCEEECCCCCHHHHHHHHHCCCHHHHCHHHHHHHHHCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA